Acid Phosphatase (ACP1) (NM_004300) Human Tagged ORF Clone

CAT#: RC212653

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ACP1 (Myc-DDK-tagged)-Human acid phosphatase 1, soluble (ACP1), transcript variant 3



  "NM_004300" in other vectors (6)

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USD 98.00

USD 390.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Acid Phosphatase (ACP1) (NM_004300) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ACP1
Synonyms HAAP; LMW-PTP; LMWPTP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC212653 representing NM_004300
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAACAGGCTACCAAGTCCGTGCTGTTTGTGTGTCTGGGTAACATTTGTCGATCACCCATTGCAG
AAGCAGTTTTCAGGAAACTTGTAACCGATCAAAACATCTCAGAGAATTGGAGGGTAGACAGCGCGGCAAC
TTCCGGGTATGAGATAGGGAACCCCCCTGACTACCGAGGGCAGAGCTGCATGAAGAGGCACGGCATTCCC
ATGAGCCACGTTGCCCGGCAGATTACCAAAGAAGATTTTGCCACATTTGATTATATACTATGTATGGATG
AAAGCAATCTGAGAGATTTGAATAGAAAAAGTAATCGAGTTAAAACCTGCAAAGCTAAAATTGAACTACT
TGGGAGCTATGATCCACAAAAACAACTTATTATTGAAGATCCCTATTATGGGAATGACTCTGACTTTGAG
ACGGTGTACCAGCAGTGTGTCAGGTGCTGCAGAGCGTTCTTGGAGAAGGCCCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC212653 representing NM_004300
Red=Cloning site Green=Tags(s)

MAEQATKSVLFVCLGNICRSPIAEAVFRKLVTDQNISENWRVDSAATSGYEIGNPPDYRGQSCMKRHGIP
MSHVARQITKEDFATFDYILCMDESNLRDLNRKSNRVKTCKAKIELLGSYDPQKQLIIEDPYYGNDSDFE
TVYQQCVRCCRAFLEKAH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004300
ORF Size 474 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004300.1, NM_004300.2, NM_004300.3, NP_004291.1
RefSeq Size 1549 bp
RefSeq ORF 477 bp
Locus ID 52
Cytogenetics 2p25.3
Domains LMWPc
Protein Families Druggable Genome, Phosphatase, Transmembrane
Protein Pathways Adherens junction, Riboflavin metabolism
MW 17.9 kDa
Gene Summary The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Aug 2008]

Other Versions

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