Espin (ESPN) (NM_031475) Human Tagged ORF Clone

CAT#: RC212577

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ESPN (Myc-DDK-tagged)-Human espin (ESPN)



  "NM_031475" in other vectors (4)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 782.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ESPN
Synonyms DFNB36; LP2654; USH1M
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC212577 representing NM_031475
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTGGAGCAGGCGCTGCAGGCGGCGCGGCAGGGCGAGCTGGACGTGCTGAGGTCGCTGCACGCCG
CAGGCCTCCTGGGGCCCTCGCTGCGCGACCCGCTGGACGCGCTGCCCGTGCACCACGCGGCCCGCGCTGG
GAAGCTGCACTGTCTGCGCTTCCTGGTGGAGGAAGCCGCCCTCCCCGCCGCGGCCCGCGCCCGCAACGGC
GCCACACCGGCCCACGACGCCTCCGCCACCGGCCACCTCGCCTGCCTGCAGTGGCTGCTGTCGCAGGGCG
GCTGCAGAGTGCAGGACAAAGACAATTCTGGTGCCACAGTCTTGCATCTGGCTGCCCGCTTCGGCCACCC
CGAGGTGGTGAACTGGCTCTTGCATCATGGCGGTGGGGACCCCACCGCGGCCACAGACATGGGCGCCCTG
CCTATCCACTACGCTGCCGCCAAAGGAGACTTCCCCTCCCTGAGGCTTCTCGTCGAGCACTACCCTGAGG
GAGTGAATGCCCAAACCAAGAACGGTGCCACGCCCCTGTACCTGGCGTGCCAGGAGGGCCACCTGGAGGT
GACCCAGTACCTGGTGCAGGAATGCGGCGCAGACCCGCACGCGCGCGCCCACGACGGCATGACCCCGCTG
CACGCCGCGGCGCAGATGGGCCACAGCCCAGTCATCGTGTGGTTGGTGAGCTGCACCGACGTGAGCCTGT
CCGAGCAGGACAAAGACGGCGCCACCGCCATGCACTTCGCGGCGAGCCGCGGCCACACCAAGGTGCTCAG
CTGGCTGCTGCTGCACGGCGGGGAGATCTCGGCTGACCTGTGGGGCGGGACCCCGCTGCACGACGCCGCC
GAGAACGGGGAGCTAGAGTGCTGCCAGATCCTGGTAGTGAACGGCGCGGAGCTGGACGTCCGCGACCGCG
ACGGGTACACGGCCGCCGACCTGTCGGACTTCAACGGCCACAGCCACTGCACCCGCTACCTGCGCACGGT
GGAGAACCTGAGCGTGGAGCACCGCGTGCTTTCCCGGGATCCATCCGCAGAGCTGGAGGCTAAGCAGCCG
GATTCAGGCATGTCCTCACCCAATACCACGGTGTCGGTCCAGCCGCTGAACTTTGACCTCAGCTCGCCTA
CCAGCACCCTCTCCAACTACGACTCCTGCTCCTCCAGCCACTCCAGCATCAAGGGCCAGCACCCTCCATG
TGGGCTTTCCAGCGCTAGAGCTGCAGACATACAGAGCTACATGGACATGCTGAACCCGGAGCTGGGCCTG
CCTCGGGGCACGATTGGGAAGCCCACACCCCCACCACCCCCACCCAGCTTCCCCCCGCCACCCCCGCCCC
CAGGCACCCAACTGCCCCCACCCCCACCTGGCTACCCAGCTCCCAAGCCTCCTGTAGGACCACAGGCAGC
TGACATCTACATGCAGACCAAGAACAAACTCCGCCACGTGGAGACAGAGGCCCTCAAGAAGGAGCTGAGC
TCCTGTGACGGCCACGACGGGCTGCGGAGGCAGGACTCCAGCCGCAAGCCCCGCGCCTTCAGCAAGCAGC
CCAGCACGGGGGACTACTACCGGCAGCTGGGCCGCTGCCCCGGCGAGACGCTGGCCGCACGCCCGGGCAT
GGCGCACAGCGAGGAGGTGCGTGCCCGCCAGCCCGCGCGCGCCGGCTGCCCGCGCCTCGGCCCTGCCGCC
CGCGGCTCACTCGAAGGCCCCTCCGCTCCCCCGCAGGCGGCGCTGCTTCCTGGGAACCATGTTCCTAACG
GCTGCGCCGCGGACCCCAAGGCGTCCAGGGAGCTGCCACCGCCGCCCCCACCGCCGCCGCCGCCCCTGCC
GGAGGCCGCGAGTTCGCCACCGCCGGCCCCGCCTCTGCCCCTCGAGAGCGCTGGCCCTGGCTGCGGGCAG
CGCCGCTCCTCCTCGTCCACCGGCAGCACCAAGTCTTTCAACATGATGTCCCCGACGGGCGACAACTCGG
AGCTACTGGCTGAGATTAAGGCAGGCAAGAGCCTGAAGCCGACGCCCCAGAGCAAGGGGCTGACCACAGT
GTTCTCAGGCATCGGGCAGCCGGCCTTCCAGCCCGATTCGCCGCTGCCTTCTGTGTCACCTGCACTGTCA
CCAGTCCGGAGCCCCACACCGCCAGCTGCGGGGTTTCAGCCGCTGCTCAATGGAAGCTTGGTTCCCGTGC
CGCCCACTACTCCTGCGCCGGGAGTGCAGCTGGACGTGGAGGCTCTCATCCCCACGCACGATGAGCAGGG
CCGGCCCATCCCCGAGTGGAAGCGCCAGGTGATGGTGCGCAAGATGCAGCTGAAGATGCAGGAGGAGGAG
GAGCAGAGGCGGAAGGAGGAGGAGGAGGAGGCCCGGCTGGCCAGCATGCCCGCCTGGAGGCGGGACCTCC
TGCGGAAGAAGCTGGAAGAAGAGAGGGAGCAGAAGCGGAAAGAGGAGGAGCGACAGAAGCAGGAGGAGCT
GCGGCGGGAGAAGGAACAGTCAGAGAAGCTGCGGACGCTGGGCTACGATGAGAGCAAGCTGGCGCCCTGG
CAGCGACAGGTCATCCTGAAGAAGGGGGACATCGCTAAGTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC212577 representing NM_031475
Red=Cloning site Green=Tags(s)

MALEQALQAARQGELDVLRSLHAAGLLGPSLRDPLDALPVHHAARAGKLHCLRFLVEEAALPAAARARNG
ATPAHDASATGHLACLQWLLSQGGCRVQDKDNSGATVLHLAARFGHPEVVNWLLHHGGGDPTAATDMGAL
PIHYAAAKGDFPSLRLLVEHYPEGVNAQTKNGATPLYLACQEGHLEVTQYLVQECGADPHARAHDGMTPL
HAAAQMGHSPVIVWLVSCTDVSLSEQDKDGATAMHFAASRGHTKVLSWLLLHGGEISADLWGGTPLHDAA
ENGELECCQILVVNGAELDVRDRDGYTAADLSDFNGHSHCTRYLRTVENLSVEHRVLSRDPSAELEAKQP
DSGMSSPNTTVSVQPLNFDLSSPTSTLSNYDSCSSSHSSIKGQHPPCGLSSARAADIQSYMDMLNPELGL
PRGTIGKPTPPPPPPSFPPPPPPPGTQLPPPPPGYPAPKPPVGPQAADIYMQTKNKLRHVETEALKKELS
SCDGHDGLRRQDSSRKPRAFSKQPSTGDYYRQLGRCPGETLAARPGMAHSEEVRARQPARAGCPRLGPAA
RGSLEGPSAPPQAALLPGNHVPNGCAADPKASRELPPPPPPPPPPLPEAASSPPPAPPLPLESAGPGCGQ
RRSSSSTGSTKSFNMMSPTGDNSELLAEIKAGKSLKPTPQSKGLTTVFSGIGQPAFQPDSPLPSVSPALS
PVRSPTPPAAGFQPLLNGSLVPVPPTTPAPGVQLDVEALIPTHDEQGRPIPEWKRQVMVRKMQLKMQEEE
EQRRKEEEEEARLASMPAWRRDLLRKKLEEEREQKRKEEERQKQEELRREKEQSEKLRTLGYDESKLAPW
QRQVILKKGDIAKY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_031475
ORF Size 2562 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_031475.3
RefSeq Size 3542 bp
RefSeq ORF 2565 bp
Locus ID 83715
UniProt ID B1AK53
Cytogenetics 1p36.31
MW 91.6 kDa
Gene Summary This gene encodes a multifunctional actin-bundling protein. It plays a major role in regulating the organization, dimensions, dynamics, and signaling capacities of the actin filament-rich, microvillus-type specializations that mediate sensory transduction in various mechanosensory and chemosensory cells. Mutations in this gene are associated with autosomal recessive neurosensory deafness, and autosomal dominant sensorineural deafness without vestibular involvement. [provided by RefSeq, Nov 2009]

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