TIF1 gamma (TRIM33) (NM_015906) Human Tagged ORF Clone

CAT#: RC212497

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TRIM33 (Myc-DDK-tagged)-Human tripartite motif containing 33 (TRIM33), transcript variant a



Reconstitution Protocol

USD 1,471.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TRIM33
Synonyms ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA; TIFGAMMA
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC212497 representing NM_015906
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAAAACAAAGGCGGCGGCGAGGCTGAGAGCGGCGGCGGGGGCAGCGGCAGCGCGCCGGTAACTG
CCGGGGCCGCCGGGCCCGCCGCGCAGGAGGCGGAGCCGCCTCTCACCGCGGTGCTGGTGGAGGAGGAGGA
GGAGGAAGGCGGCAGGGCCGGCGCTGAGGGCGGCGCGGCCGGGCCCGACGACGGGGGGGTGGCCGCGGCC
TCCTCGGGCTCGGCCCAGGCTGCTTCATCTCCTGCGGCCTCAGTGGGCACTGGAGTTGCCGGGGGCGCAG
TATCGACGCCGGCTCCAGCTCCAGCCTCGGCTCCCGCTCCGGGTCCCTCGGCAGGGCCGCCTCCTGGACC
GCCAGCCTCGCTCCTGGACACCTGCGCCGTGTGTCAGCAGAGCTTGCAGAGCCGGCGTGAGGCGGAGCCC
AAGCTGCTGCCCTGTCTTCACTCCTTCTGCCTGCGCTGCCTGCCCGAGCCGGAGCGCCAGCTCAGCGTGC
CCATCCCGGGGGGCAGCAACGGCGACATCCAGCAAGTTGGTGTAATACGGTGCCCAGTATGCCGCCAAGA
ATGCAGACAGATAGACCTTGTGGATAATTATTTTGTGAAAGACACATCTGAAGCTCCTAGCAGTTCTGAT
GAAAAATCAGAACAGGTATGTACTAGTTGTGAAGACAATGCAAGTGCAGTTGGCTTTTGTGTAGAATGTG
GAGAGTGGCTATGTAAGACATGTATCGAAGCACATCAAAGAGTAAAATTTACTAAAGATCACTTGATCAG
GAAGAAAGAAGATGTCTCAGAGTCTGTTGGAGCATCTGGTCAACGCCCTGTTTTCTGCCCTGTACACAAA
CAAGAACAGTTGAAACTTTTCTGTGAAACATGTGATAGATTGACATGTAGAGACTGTCAGCTATTGGAAC
ACAAAGAACATAGGTATCAGTTTTTGGAAGAAGCTTTTCAAAATCAGAAGGGTGCAATTGAGAATCTACT
GGCGAAACTTCTTGAGAAGAAGAATTATGTTCATTTTGCAGCTACTCAGGTGCAGAATAGGATAAAAGAA
GTAAATGAGACTAACAAACGAGTAGAACAGGAAATTAAAGTGGCCATTTTCACCCTTATCAATGAAATTA
ATAAGAAAGGAAAATCTCTCTTACAACAGCTAGAGAATGTTACAAAGGAAAGACAGATGAAGTTACTACA
GCAGCAGAATGACATCACAGGCCTTTCCCGGCAGGTGAAGCATGTTATGAACTTCACAAATTGGGCAATT
GCAAGTGGCAGCAGCACAGCACTACTATACAGCAAGCGACTGATTACTTTCCAGTTGCGTCATATTTTGA
AAGCACGGTGTGATCCTGTCCCTGCTGCTAATGGAGCAATACGTTTCCATTGTGATCCCACCTTCTGGGC
AAAGAATGTAGTCAATTTAGGTAATCTAGTAATAGAGAGTAAACCAGCTCCTGGTTATACTCCTAATGTT
GTAGTTGGGCAAGTTCCTCCAGGGACAAACCACATTAGTAAAACCCCTGGACAGATTAACTTAGCACAGC
TTCGACTCCAGCACATGCAACAACAAGTATATGCACAGAAACATCAGCAGTTGCAACAGATGAGGATGCA
GCAACCACCAGCACCTGTACCAACTACAACAACAACAACACAACAGCATCCTAGACAAGCAGCCCCTCAG
ATGTTACAACAACAGCCTCCTCGATTGATCAGTGTGCAAACAATGCAAAGAGGCAACATGAACTGTGGAG
CTTTTCAAGCCCATCAGATGAGACTGGCTCAGAATGCTGCCAGAATACCAGGGATACCCAGGCACAGCGG
CCCTCAATATTCCATGATGCAGCCACACCTCCAAAGACAACACTCAAACCCAGGGCATGCTGGACCCTTT
CCCGTAGTATCGGTACACAACACCACAATCAACCCAACGAGCCCTACTACAGCAACTATGGCAAATGCAA
ACCGAGGTCCCACCAGCCCATCTGTTACAGCAATAGAGCTAATCCCCTCAGTTACCAATCCAGAAAACCT
TCCATCGCTGCCAGATATTCCACCCATACAGTTGGAAGATGCTGGCTCAAGTAGTTTAGATAATCTACTA
AGTAGATACATCTCAGGCAGTCACCTACCCCCACAGCCTACAAGCACCATGAATCCTTCTCCAGGTCCCT
CTGCCCTTTCTCCGGGATCATCAGGTTTATCCAATTCTCACACACCTGTGAGACCCCCAAGTACTTCTAG
TACTGGCAGTCGAGGCAGCTGTGGGTCATCAGGAAGAACTGCTGAGAAGACAAGTCTTAGTTTCAAATCT
GATCAGGTGAAGGTCAAGCAAGAACCTGGGACTGAAGATGAAATATGTAGCTTTTCAGGAGGTGTAAAAC
AAGAAAAAACAGAGGATGGCAGGAGGAGTGCTTGCATGTTGAGCAGTCCTGAGAGTAGCTTGACACCACC
TCTCTCAACCAACCTGCATCTAGAAAGTGAATTGGATGCATTGGCAAGCCTGGAAAACCATGTGAAAACT
GAACCTGCAGATATGAATGAAAGCTGCAAACAGTCAGGGCTCAGCAGCCTTGTTAATGGAAAGTCCCCAA
TTCGAAGCCTCATGCACAGGTCGGCAAGGATTGGAGGAGATGGCAACAATAAAGATGATGACCCAAATGA
AGACTGGTGTGCTGTCTGCCAAAACGGAGGAGATCTCTTGTGCTGCGAAAAATGTCCAAAGGTCTTTCAT
CTAACTTGTCATGTTCCAACACTACTTAGCTTTCCAAGTGGGGACTGGATATGCACATTTTGTAGAGATA
TTGGAAAGCCAGAAGTTGAATATGATTGTGATAATTTGCAACATAGTAAGAAGGGGAAAACTGCGCAGGG
GTTAAGCCCCGTGGACCAAAGGAAATGTGAACGTCTTCTGCTTTACCTCTATTGCCATGAATTAAGTATT
GAATTCCAGGAGCCTGTTCCTGCTTCGATACCAAACTACTATAAAATTATAAAGAAACCAATGGATTTAT
CCACCGTGAAAAAGAAGCTTCAGAAAAAACATTCCCAACACTACCAAATCCCGGATGACTTTGTGGCCGA
TGTCCGTTTGATCTTCAAGAACTGTGAAAGGTTTAATGAAATGATGAAAGTTGTTCAAGTTTATGCAGAC
ACACAAGAGATTAATTTGAAGGCTGATTCAGAAGTAGCTCAGGCAGGGAAAGCAGTTGCATTGTACTTTG
AAGATAAACTCACAGAGATCTACTCAGACAGGACCTTCGCACCTTTGCCAGAGTTTGAGCAGGAAGAGGA
TGATGGTGAGGTAACTGAGGACTCTGATGAAGACTTTATACAGCCCCGCAGAAAACGCCTAAAGTCAGAT
GAGAGACCAGTACATATAAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC212497 representing NM_015906
Red=Cloning site Green=Tags(s)

MAENKGGGEAESGGGGSGSAPVTAGAAGPAAQEAEPPLTAVLVEEEEEEGGRAGAEGGAAGPDDGGVAAA
SSGSAQAASSPAASVGTGVAGGAVSTPAPAPASAPAPGPSAGPPPGPPASLLDTCAVCQQSLQSRREAEP
KLLPCLHSFCLRCLPEPERQLSVPIPGGSNGDIQQVGVIRCPVCRQECRQIDLVDNYFVKDTSEAPSSSD
EKSEQVCTSCEDNASAVGFCVECGEWLCKTCIEAHQRVKFTKDHLIRKKEDVSESVGASGQRPVFCPVHK
QEQLKLFCETCDRLTCRDCQLLEHKEHRYQFLEEAFQNQKGAIENLLAKLLEKKNYVHFAATQVQNRIKE
VNETNKRVEQEIKVAIFTLINEINKKGKSLLQQLENVTKERQMKLLQQQNDITGLSRQVKHVMNFTNWAI
ASGSSTALLYSKRLITFQLRHILKARCDPVPAANGAIRFHCDPTFWAKNVVNLGNLVIESKPAPGYTPNV
VVGQVPPGTNHISKTPGQINLAQLRLQHMQQQVYAQKHQQLQQMRMQQPPAPVPTTTTTTQQHPRQAAPQ
MLQQQPPRLISVQTMQRGNMNCGAFQAHQMRLAQNAARIPGIPRHSGPQYSMMQPHLQRQHSNPGHAGPF
PVVSVHNTTINPTSPTTATMANANRGPTSPSVTAIELIPSVTNPENLPSLPDIPPIQLEDAGSSSLDNLL
SRYISGSHLPPQPTSTMNPSPGPSALSPGSSGLSNSHTPVRPPSTSSTGSRGSCGSSGRTAEKTSLSFKS
DQVKVKQEPGTEDEICSFSGGVKQEKTEDGRRSACMLSSPESSLTPPLSTNLHLESELDALASLENHVKT
EPADMNESCKQSGLSSLVNGKSPIRSLMHRSARIGGDGNNKDDDPNEDWCAVCQNGGDLLCCEKCPKVFH
LTCHVPTLLSFPSGDWICTFCRDIGKPEVEYDCDNLQHSKKGKTAQGLSPVDQRKCERLLLYLYCHELSI
EFQEPVPASIPNYYKIIKKPMDLSTVKKKLQKKHSQHYQIPDDFVADVRLIFKNCERFNEMMKVVQVYAD
TQEINLKADSEVAQAGKAVALYFEDKLTEIYSDRTFAPLPEFEQEEDDGEVTEDSDEDFIQPRRKRLKSD
ERPVHIK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_015906
ORF Size 3381 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015906.4
RefSeq Size 8339 bp
RefSeq ORF 3384 bp
Locus ID 51592
UniProt ID Q9UPN9
Cytogenetics 1p13.2
Domains zf-B_box, BROMO, RING, PHD, BBC
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transcription Factors
MW 122.4 kDa
Gene Summary The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]
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