TIF1 gamma (TRIM33) (NM_015906) Human Tagged ORF Clone

CAT#: RC212497

TRIM33 (Myc-DDK-tagged)-Human tripartite motif containing 33 (TRIM33), transcript variant a

Product Images

Circular map for RC212497

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY Trim33 (Cat# RC212497, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Trim33(Cat# TA500119). Positive lysates LY414337 (100ug) and LC414337 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY414337) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212497 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified TRIM33 protein (Cat# TP312497). The protein was produced from HEK293T cells transfected with TRIM33 cDNA clone (Cat# RC212497) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TIF1 gamma
• Synonyms: ECTO; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA; TIFGAMMA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212497 representing NM_015906
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAAACAAAGGCGGCGGCGAGGCTGAGAGCGGCGGCGGGGGCAGCGGCAGCGCGCCGGTAACTG
  CCGGGGCCGCCGGGCCCGCCGCGCAGGAGGCGGAGCCGCCTCTCACCGCGGTGCTGGTGGAGGAGGAGGA
  GGAGGAAGGCGGCAGGGCCGGCGCTGAGGGCGGCGCGGCCGGGCCCGACGACGGGGGGGTGGCCGCGGCC
  TCCTCGGGCTCGGCCCAGGCTGCTTCATCTCCTGCGGCCTCAGTGGGCACTGGAGTTGCCGGGGGCGCAG
  TATCGACGCCGGCTCCAGCTCCAGCCTCGGCTCCCGCTCCGGGTCCCTCGGCAGGGCCGCCTCCTGGACC
  GCCAGCCTCGCTCCTGGACACCTGCGCCGTGTGTCAGCAGAGCTTGCAGAGCCGGCGTGAGGCGGAGCCC
  AAGCTGCTGCCCTGTCTTCACTCCTTCTGCCTGCGCTGCCTGCCCGAGCCGGAGCGCCAGCTCAGCGTGC
  CCATCCCGGGGGGCAGCAACGGCGACATCCAGCAAGTTGGTGTAATACGGTGCCCAGTATGCCGCCAAGA
  ATGCAGACAGATAGACCTTGTGGATAATTATTTTGTGAAAGACACATCTGAAGCTCCTAGCAGTTCTGAT
  GAAAAATCAGAACAGGTATGTACTAGTTGTGAAGACAATGCAAGTGCAGTTGGCTTTTGTGTAGAATGTG
  GAGAGTGGCTATGTAAGACATGTATCGAAGCACATCAAAGAGTAAAATTTACTAAAGATCACTTGATCAG
  GAAGAAAGAAGATGTCTCAGAGTCTGTTGGAGCATCTGGTCAACGCCCTGTTTTCTGCCCTGTACACAAA
  CAAGAACAGTTGAAACTTTTCTGTGAAACATGTGATAGATTGACATGTAGAGACTGTCAGCTATTGGAAC
  ACAAAGAACATAGGTATCAGTTTTTGGAAGAAGCTTTTCAAAATCAGAAGGGTGCAATTGAGAATCTACT
  GGCGAAACTTCTTGAGAAGAAGAATTATGTTCATTTTGCAGCTACTCAGGTGCAGAATAGGATAAAAGAA
  GTAAATGAGACTAACAAACGAGTAGAACAGGAAATTAAAGTGGCCATTTTCACCCTTATCAATGAAATTA
  ATAAGAAAGGAAAATCTCTCTTACAACAGCTAGAGAATGTTACAAAGGAAAGACAGATGAAGTTACTACA
  GCAGCAGAATGACATCACAGGCCTTTCCCGGCAGGTGAAGCATGTTATGAACTTCACAAATTGGGCAATT
  GCAAGTGGCAGCAGCACAGCACTACTATACAGCAAGCGACTGATTACTTTCCAGTTGCGTCATATTTTGA
  AAGCACGGTGTGATCCTGTCCCTGCTGCTAATGGAGCAATACGTTTCCATTGTGATCCCACCTTCTGGGC
  AAAGAATGTAGTCAATTTAGGTAATCTAGTAATAGAGAGTAAACCAGCTCCTGGTTATACTCCTAATGTT
  GTAGTTGGGCAAGTTCCTCCAGGGACAAACCACATTAGTAAAACCCCTGGACAGATTAACTTAGCACAGC
  TTCGACTCCAGCACATGCAACAACAAGTATATGCACAGAAACATCAGCAGTTGCAACAGATGAGGATGCA
  GCAACCACCAGCACCTGTACCAACTACAACAACAACAACACAACAGCATCCTAGACAAGCAGCCCCTCAG
  ATGTTACAACAACAGCCTCCTCGATTGATCAGTGTGCAAACAATGCAAAGAGGCAACATGAACTGTGGAG
  CTTTTCAAGCCCATCAGATGAGACTGGCTCAGAATGCTGCCAGAATACCAGGGATACCCAGGCACAGCGG
  CCCTCAATATTCCATGATGCAGCCACACCTCCAAAGACAACACTCAAACCCAGGGCATGCTGGACCCTTT
  CCCGTAGTATCGGTACACAACACCACAATCAACCCAACGAGCCCTACTACAGCAACTATGGCAAATGCAA
  ACCGAGGTCCCACCAGCCCATCTGTTACAGCAATAGAGCTAATCCCCTCAGTTACCAATCCAGAAAACCT
  TCCATCGCTGCCAGATATTCCACCCATACAGTTGGAAGATGCTGGCTCAAGTAGTTTAGATAATCTACTA
  AGTAGATACATCTCAGGCAGTCACCTACCCCCACAGCCTACAAGCACCATGAATCCTTCTCCAGGTCCCT
  CTGCCCTTTCTCCGGGATCATCAGGTTTATCCAATTCTCACACACCTGTGAGACCCCCAAGTACTTCTAG
  TACTGGCAGTCGAGGCAGCTGTGGGTCATCAGGAAGAACTGCTGAGAAGACAAGTCTTAGTTTCAAATCT
  GATCAGGTGAAGGTCAAGCAAGAACCTGGGACTGAAGATGAAATATGTAGCTTTTCAGGAGGTGTAAAAC
  AAGAAAAAACAGAGGATGGCAGGAGGAGTGCTTGCATGTTGAGCAGTCCTGAGAGTAGCTTGACACCACC
  TCTCTCAACCAACCTGCATCTAGAAAGTGAATTGGATGCATTGGCAAGCCTGGAAAACCATGTGAAAACT
  GAACCTGCAGATATGAATGAAAGCTGCAAACAGTCAGGGCTCAGCAGCCTTGTTAATGGAAAGTCCCCAA
  TTCGAAGCCTCATGCACAGGTCGGCAAGGATTGGAGGAGATGGCAACAATAAAGATGATGACCCAAATGA
  AGACTGGTGTGCTGTCTGCCAAAACGGAGGAGATCTCTTGTGCTGCGAAAAATGTCCAAAGGTCTTTCAT
  CTAACTTGTCATGTTCCAACACTACTTAGCTTTCCAAGTGGGGACTGGATATGCACATTTTGTAGAGATA
  TTGGAAAGCCAGAAGTTGAATATGATTGTGATAATTTGCAACATAGTAAGAAGGGGAAAACTGCGCAGGG
  GTTAAGCCCCGTGGACCAAAGGAAATGTGAACGTCTTCTGCTTTACCTCTATTGCCATGAATTAAGTATT
  GAATTCCAGGAGCCTGTTCCTGCTTCGATACCAAACTACTATAAAATTATAAAGAAACCAATGGATTTAT
  CCACCGTGAAAAAGAAGCTTCAGAAAAAACATTCCCAACACTACCAAATCCCGGATGACTTTGTGGCCGA
  TGTCCGTTTGATCTTCAAGAACTGTGAAAGGTTTAATGAAATGATGAAAGTTGTTCAAGTTTATGCAGAC
  ACACAAGAGATTAATTTGAAGGCTGATTCAGAAGTAGCTCAGGCAGGGAAAGCAGTTGCATTGTACTTTG
  AAGATAAACTCACAGAGATCTACTCAGACAGGACCTTCGCACCTTTGCCAGAGTTTGAGCAGGAAGAGGA
  TGATGGTGAGGTAACTGAGGACTCTGATGAAGACTTTATACAGCCCCGCAGAAAACGCCTAAAGTCAGAT
  GAGAGACCAGTACATATAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212497 representing NM_015906
  Red=Cloning site Green=Tags(s)
  
  MAENKGGGEAESGGGGSGSAPVTAGAAGPAAQEAEPPLTAVLVEEEEEEGGRAGAEGGAAGPDDGGVAAA
  SSGSAQAASSPAASVGTGVAGGAVSTPAPAPASAPAPGPSAGPPPGPPASLLDTCAVCQQSLQSRREAEP
  KLLPCLHSFCLRCLPEPERQLSVPIPGGSNGDIQQVGVIRCPVCRQECRQIDLVDNYFVKDTSEAPSSSD
  EKSEQVCTSCEDNASAVGFCVECGEWLCKTCIEAHQRVKFTKDHLIRKKEDVSESVGASGQRPVFCPVHK
  QEQLKLFCETCDRLTCRDCQLLEHKEHRYQFLEEAFQNQKGAIENLLAKLLEKKNYVHFAATQVQNRIKE
  VNETNKRVEQEIKVAIFTLINEINKKGKSLLQQLENVTKERQMKLLQQQNDITGLSRQVKHVMNFTNWAI
  ASGSSTALLYSKRLITFQLRHILKARCDPVPAANGAIRFHCDPTFWAKNVVNLGNLVIESKPAPGYTPNV
  VVGQVPPGTNHISKTPGQINLAQLRLQHMQQQVYAQKHQQLQQMRMQQPPAPVPTTTTTTQQHPRQAAPQ
  MLQQQPPRLISVQTMQRGNMNCGAFQAHQMRLAQNAARIPGIPRHSGPQYSMMQPHLQRQHSNPGHAGPF
  PVVSVHNTTINPTSPTTATMANANRGPTSPSVTAIELIPSVTNPENLPSLPDIPPIQLEDAGSSSLDNLL
  SRYISGSHLPPQPTSTMNPSPGPSALSPGSSGLSNSHTPVRPPSTSSTGSRGSCGSSGRTAEKTSLSFKS
  DQVKVKQEPGTEDEICSFSGGVKQEKTEDGRRSACMLSSPESSLTPPLSTNLHLESELDALASLENHVKT
  EPADMNESCKQSGLSSLVNGKSPIRSLMHRSARIGGDGNNKDDDPNEDWCAVCQNGGDLLCCEKCPKVFH
  LTCHVPTLLSFPSGDWICTFCRDIGKPEVEYDCDNLQHSKKGKTAQGLSPVDQRKCERLLLYLYCHELSI
  EFQEPVPASIPNYYKIIKKPMDLSTVKKKLQKKHSQHYQIPDDFVADVRLIFKNCERFNEMMKVVQVYAD
  TQEINLKADSEVAQAGKAVALYFEDKLTEIYSDRTFAPLPEFEQEEDDGEVTEDSDEDFIQPRRKRLKSD
  ERPVHIK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015906
• ORF Size: 3381 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015906.4
• RefSeq Size: 8339 bp
• RefSeq ORF: 3384 bp
• Locus ID: 51592
• UniProt ID: Q9UPN9
• Cytogenetics: 1p13.2
• Domains: zf-B_box, BROMO, RING, PHD, BBC
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transcription Factors
• MW: 122.4 kDa
• Gene Summary: The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]