EEF2 (NM_001961) Human Tagged ORF Clone

CAT#: RC212420

EEF2 (Myc-DDK-tagged)-Human eukaryotic translation elongation factor 2 (EEF2)

Product Images

Circular map for RC212420

• 

  Western blot validation of overexpression lysate (Cat# LY419622) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212420 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified EEF2 protein (Cat# TP312420). The protein was produced from HEK293T cells transfected with EEF2 cDNA clone (Cat# RC212420) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EEF2
• Synonyms: EEF-2; EF-2; EF2; SCA26
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212420 representing NM_001961
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAACTTCACGGTAGACCAGATCCGCGCCATCATGGACAAGAAGGCCAACATCCGCAACATGTCTG
  TCATCGCCCACGTGGACCATGGCAAGTCCACGCTGACAGACTCCCTGGTGTGCAAGGCGGGCATCATCGC
  CTCGGCCCGGGCCGGGGAGACACGCTTCACTGATACCCGGAAGGACGAGCAGGAGCGTTGCATCACCATC
  AAGTCAACTGCCATCTCCCTCTTCTACGAGCTCTCGGAGAATGACTTGAACTTCATCAAGCAGAGCAAGG
  ACGGTGCCGGCTTCCTCATCAACCTCATTGACTCCCCCGGGCATGTCGACTTCTCCTCGGAGGTGACTGC
  TGCCCTCCGAGTCACCGATGGCGCATTGGTGGTGGTGGACTGCGTGTCAGGCGTGTGCGTGCAGACGGAG
  ACAGTGCTGCGGCAGGCCATTGCCGAGCGCATCAAGCCTGTGCTGATGATGAACAAGATGGACCGCGCCC
  TGCTGGAGCTGCAGCTGGAGCCCGAGGAGCTCTACCAGACTTTCCAGCGCATCGTGGAGAACGTGAACGT
  CATCATCTCCACCTACGGCGAGGGCGAGAGCGGCCCCATGGGCAACATCATGATCGATCCTGTCCTCGGT
  ACCGTGGGCTTTGGGTCTGGCCTCCACGGGTGGGCCTTCACCCTGAAGCAGTTTGCCGAGATGTATGTGG
  CCAAGTTCGCCGCCAAGGGGGAGGGCCAGTTGGGGCCTGCCGAGCGGGCCAAGAAAGTAGAGGACATGAT
  GAAGAAGCTGTGGGGTGACAGGTACTTTGACCCAGCCAACGGCAAGTTCAGCAAGTCAGCCACCAGCCCC
  GAAGGGAAGAAGCTGCCACGCACCTTCTGCCAGCTGATCCTGGACCCCATCTTCAAGGTGTTTGATGCGA
  TCATGAATTTCAAGAAAGAGGAGACAGCAAAACTGATAGAGAAACTGGACATCAAACTGGACAGCGAGGA
  CAAGGACAAAGAAGGCAAACCCCTGCTGAAGGCTGTGATGCGCCGCTGGCTGCCTGCCGGAGACGCCTTG
  TTGCAGATGATCACCATCCACCTGCCCTCCCCTGTGACGGCCCAGAAGTACCGCTGCGAGCTCCTGTACG
  AGGGGCCCCCGGACGACGAGGCTGCCATGGGCATTAAAAGCTGTGACCCCAAAGGCCCTCTTATGATGTA
  TATTTCCAAAATGGTGCCAACCTCCGACAAAGGTCGGTTCTACGCCTTTGGACGAGTCTTCTCGGGGCTG
  GTCTCCACTGGCCTGAAGGTCAGGATCATGGGGCCCAACTATACCCCTGGGAAGAAGGAGGACCTCTACC
  TGAAGCCAATCCAGAGAACAATCTTGATGATGGGCCGCTACGTGGAGCCCATCGAGGATGTGCCTTGTGG
  GAACATTGTGGGCCTCGTGGGCGTGGACCAGTTCCTGGTGAAGACGGGCACCATCACCACCTTCGAGCAC
  GCGCACAACATGCGGGTGATGAAGTTCAGCGTCAGCCCTGTTGTCAGAGTGGCCGTGGAGGCCAAGAACC
  CGGCTGACCTGCCCAAGCTGGTGGAGGGGCTGAAGCGGCTGGCCAAGTCCGACCCCATGGTGCAGTGCAT
  CATCGAGGAGTCGGGAGAGCACATCATCGCGGGCGCCGGCGAGCTGCACCTGGAGATCTGCCTGAAGGAC
  CTGGAGGAGGACCACGCCTGCATCCCCATCAAGAAATCTGACCCGGTCGTCTCGTACCGCGAGACGGTCA
  GTGAAGAGTCGAACGTGCTCTGCCTCTCCAAGTCCCCCAACAAGCACAACCGGCTGTACATGAAGGCGCG
  GCCCTTCCCCGACGGCCTGGCCGAGGACATCGATAAAGGCGAGGTGTCCGCCCGTCAGGAGCTCAAGCAG
  CGGGCGCGCTACCTGGCCGAGAAGTACGAGTGGGACGTGGCTGAGGCCCGCAAGATCTGGTGCTTTGGGC
  CCGACGGCACCGGCCCCAACATCCTCACCGACATCACCAAGGGTGTGCAGTACCTCAACGAGATCAAGGA
  CAGTGTGGTGGCCGGCTTCCAGTGGGCCACCAAGGAGGGCGCACTGTGTGAGGAGAACATGCGGGGTGTG
  CGCTTCGACGTCCACGACGTCACCCTGCACGCCGACGCCATCCACCGCGGAGGGGGCCAGATCATCCCCA
  CAGCACGGCGCTGCCTCTACGCCAGTGTGCTGACCGCCCAGCCACGCCTCATGGAGCCCATCTACCTTGT
  GGAGATCCAGTGTCCAGAGCAGGTGGTCGGTGGCATCTACGGGGTTTTGAACAGGAAGCGGGGCCACGTG
  TTCGAGGAGTCCCAGGTGGCCGGCACCCCCATGTTTGTGGTCAAGGCCTATCTGCCCGTCAACGAGTCCT
  TTGGCTTCACCGCTGACCTGAGGTCCAACACGGGCGGCCAGGCGTTCCCCCAGTGTGTGTTTGACCACTG
  GCAGATCCTGCCCGGAGACCCCTTCGACAACAGCAGCCGCCCCAGCCAGGTGGTGGCGGAGACCCGCAAG
  CGCAAGGGCCTGAAAGAAGGCATCCCTGCCCTGGACAACTTCCTGGACAAATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212420 representing NM_001961
  Red=Cloning site Green=Tags(s)
  
  MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITI
  KSTAISLFYELSENDLNFIKQSKDGAGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTE
  TVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLG
  TVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLGPAERAKKVEDMMKKLWGDRYFDPANGKFSKSATSP
  EGKKLPRTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDAL
  LQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGL
  VSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEH
  AHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKD
  LEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQ
  RARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGV
  RFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHV
  FEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRK
  RKGLKEGIPALDNFLDKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001961
• ORF Size: 2574 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001961.2, NP_001952.1
• RefSeq Size: 3148 bp
• RefSeq ORF: 2577 bp
• Locus ID: 1938
• UniProt ID: P13639
• Cytogenetics: 19p13.3
• Domains: EFG_C, GTP_EFTU, GTP_EFTU_D2, EFG_IV
• MW: 95.2 kDa
• Gene Summary: This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]