MSH3 (NM_002439) Human Tagged ORF Clone

CAT#: RC212391

MSH3 (Myc-DDK-tagged)-Human mutS homolog 3 (E. coli) (MSH3)

Product Images

Circular map for RC212391

• 

  Western blot validation of overexpression lysate (Cat# LY419328) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212391 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MSH3
• Synonyms: DUP; FAP4; MRP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212391 representing NM_002439
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCGCCGGAAGCCTGCGTCGGGCGGCCTCGCTGCCTCCAGCTCAGCCCCTGCGAGGCAAGCGGTTT
  TGAGCCGATTCTTCCAGTCTACGGGAAGCCTGAAATCCACCTCCTCCTCCACAGGTGCAGCCGACCAGGT
  GGACCCTGGCGCTGCAGCGGCTGCAGCGGCCGCAGCGGCCGCAGCGCCCCCAGCGCCCCCAGCTCCCGCC
  TTCCCGCCCCAGCTGCCGCCGCACATAGCTACAGAAATTGACAGAAGAAAGAAGAGACCATTGGAAAATG
  ATGGGCCTGTTAAAAAGAAAGTAAAGAAAGTCCAACAAAAGGAAGGAGGAAGTGATCTGGGAATGTCTGG
  CAACTCTGAGCCAAAGAAATGTCTGAGGACCAGGAATGTTTCAAAGTCTCTGGAAAAATTGAAAGAATTC
  TGCTGCGATTCTGCCCTTCCTCAAAGTAGAGTCCAGACAGAATCTCTGCAGGAGAGATTTGCAGTTCTGC
  CAAAATGTACTGATTTTGATGATATCAGTCTTCTACACGCAAAGAATGCAGTTTCTTCTGAAGATTCGAA
  ACGTCAAATTAATCAAAAGGACACAACACTTTTTGATCTCAGTCAGTTTGGATCATCAAATACAAGTCAT
  GAAAATTTACAGAAAACTGCTTCCAAATCAGCTAACAAACGGTCCAAAAGCATCTATACGCCGCTAGAAT
  TACAATACATAGAAATGAAGCAGCAGCACAAAGATGCAGTTTTGTGTGTGGAATGTGGATATAAGTATAG
  ATTCTTTGGGGAAGATGCAGAGATTGCAGCCCGAGAGCTCAATATTTATTGCCATTTAGATCACAACTTT
  ATGACAGCAAGTATACCTACTCACAGACTGTTTGTTCATGTACGCCGCCTGGTGGCAAAAGGATATAAGG
  TGGGAGTTGTGAAGCAAACTGAAACTGCAGCATTAAAGGCCATTGGAGACAACAGAAGTTCACTCTTTTC
  CCGGAAATTGACTGCCCTTTATACAAAATCTACACTTATTGGAGAAGATGTGAATCCCCTAATCAAGCTG
  GATGATGCTGTAAATGTTGATGAGATAATGACTGATACTTCTACCAGCTATCTTCTGTGCATCTCTGAAA
  ATAAGGAAAATGTTAGGGACAAAAAAAAGGGCAACATTTTTATTGGCATTGTGGGAGTGCAGCCTGCCAC
  AGGCGAGGTTGTGTTTGATAGTTTCCAGGACTCTGCTTCTCGTTCAGAGCTAGAAACCCGGATGTCAAGC
  CTGCAGCCAGTAGAGCTGCTGCTTCCTTCGGCCTTGTCCGAGCAAACAGAGGCGCTCATCCACAGAGCCA
  CATCTGTTAGTGTGCAGGATGACAGAATTCGAGTCGAAAGGATGGATAACATTTATTTTGAATACAGCCA
  TGCTTTCCAGGCAGTTACAGAGTTTTATGCAAAAGATACAGTTGACATCAAAGGTTCTCAAATTATTTCT
  GGCATTGTTAACTTAGAGAAGCCTGTGATTTGCTCTTTGGCTGCCATCATAAAATACCTCAAAGAATTCA
  ACTTGGAAAAGATGCTCTCCAAACCTGAGAATTTTAAACAGCTATCAAGTAAAATGGAATTTATGACAAT
  TAATGGAACAACATTAAGGAATCTGGAAATCCTACAGAATCAGACTGATATGAAAACCAAAGGAAGTTTG
  CTGTGGGTTTTAGACCACACTAAAACTTCATTTGGGAGACGGAAGTTAAAGAAGTGGGTGACCCAGCCAC
  TCCTTAAATTAAGGGAAATAAATGCCCGGCTTGATGCTGTATCGGAAGTTCTCCATTCAGAATCTAGTGT
  GTTTGGTCAGATAGAAAATCATCTACGTAAATTGCCCGACATAGAGAGGGGACTCTGTAGCATTTATCAC
  AAAAAATGTTCTACCCAAGAGTTCTTCTTGATTGTCAAAACTTTATATCACCTAAAGTCAGAATTTCAAG
  CAATAATACCTGCTGTTAATTCCCACATTCAGTCAGACTTGCTCCGGACCGTTATTTTAGAAATTCCTGA
  ACTCCTCAGTCCAGTGGAGCATTACTTAAAGATACTCAATGAACAAGCTGCCAAAGTTGGGGATAAAACT
  GAATTATTTAAAGACCTTTCTGACTTCCCTTTAATAAAAAAGAGGAAGGATGAAATTCAAGGTGTTATTG
  ACGAGATCCGAATGCATTTGCAAGAAATACGAAAAATACTAAAAAATCCTTCTGCACAATATGTGACAGT
  ATCAGGACAGGAGTTTATGATAGAAATAAAGAACTCTGCTGTATCTTGTATACCAACTGATTGGGTAAAG
  GTTGGAAGCACAAAAGCTGTGAGCCGCTTTCACTCTCCTTTTATTGTAGAAAATTACAGACATCTGAATC
  AGCTCCGGGAGCAGCTAGTCCTTGACTGCAGTGCTGAATGGCTTGATTTTCTAGAGAAATTCAGTGAACA
  TTATCACTCCTTGTGTAAAGCAGTGCATCACCTAGCAACTGTTGACTGCATTTTCTCCCTGGCCAAGGTC
  GCTAAGCAAGGAGATTACTGCAGACCAACTGTACAAGAAGAAAGAAAAATTGTAATAAAAAATGGAAGGC
  ACCCTGTGATTGATGTGTTGCTGGGAGAACAGGATCAATATGTCCCAAATAATACAGATTTATCAGAGGA
  CTCAGAGAGAGTAATGATAATTACCGGACCAAACATGGGTGGAAAGAGCTCCTACATAAAACAAGTTGCA
  TTGATTACCATCATGGCTCAGATTGGCTCCTATGTTCCTGCAGAAGAAGCGACAATTGGGATTGTGGATG
  GCATTTTCACAAGGATGGGTGCTGCAGACAATATATATAAAGGACAGAGTACATTTATGGAAGAACTGAC
  TGACACAGCAGAAATAATCAGAAAAGCAACATCACAGTCCTTGGTTATCTTGGATGAACTAGGAAGAGGG
  ACGAGCACTCATGATGGAATTGCCATTGCCTATGCTACACTTGAGTATTTCATCAGAGATGTGAAATCCT
  TAACCCTGTTTGTCACCCATTATCCGCCAGTTTGTGAACTAGAAAAAAATTACTCACACCAGGTGGGGAA
  TTACCACATGGGATTCTTGGTCAGTGAGGATGAAAGCAAACTGGATCCAGGCGCAGCAGAACAAGTCCCT
  GATTTTGTCACCTTCCTTTACCAAATAACTAGAGGAATTGCAGCAAGGAGTTATGGATTAAATGTGGCTA
  AACTAGCAGATGTTCCTGGAGAAATTTTGAAGAAAGCAGCTCACAAGTCAAAAGAGCTGGAAGGATTAAT
  AAATACGAAAAGAAAGAGACTCAAGTATTTTGCAAAGTTATGGACGATGCATAATGCACAAGACCTGCAG
  AAGTGGACAGAGGAGTTCAACATGGAAGAAACACAGACTTCTCTTCTTCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212391 representing NM_002439
  Red=Cloning site Green=Tags(s)
  
  MSRRKPASGGLAASSSAPARQAVLSRFFQSTGSLKSTSSSTGAADQVDPGAAAAAAAAAAAAPPAPPAPA
  FPPQLPPHIATEIDRRKKRPLENDGPVKKKVKKVQQKEGGSDLGMSGNSEPKKCLRTRNVSKSLEKLKEF
  CCDSALPQSRVQTESLQERFAVLPKCTDFDDISLLHAKNAVSSEDSKRQINQKDTTLFDLSQFGSSNTSH
  ENLQKTASKSANKRSKSIYTPLELQYIEMKQQHKDAVLCVECGYKYRFFGEDAEIAARELNIYCHLDHNF
  MTASIPTHRLFVHVRRLVAKGYKVGVVKQTETAALKAIGDNRSSLFSRKLTALYTKSTLIGEDVNPLIKL
  DDAVNVDEIMTDTSTSYLLCISENKENVRDKKKGNIFIGIVGVQPATGEVVFDSFQDSASRSELETRMSS
  LQPVELLLPSALSEQTEALIHRATSVSVQDDRIRVERMDNIYFEYSHAFQAVTEFYAKDTVDIKGSQIIS
  GIVNLEKPVICSLAAIIKYLKEFNLEKMLSKPENFKQLSSKMEFMTINGTTLRNLEILQNQTDMKTKGSL
  LWVLDHTKTSFGRRKLKKWVTQPLLKLREINARLDAVSEVLHSESSVFGQIENHLRKLPDIERGLCSIYH
  KKCSTQEFFLIVKTLYHLKSEFQAIIPAVNSHIQSDLLRTVILEIPELLSPVEHYLKILNEQAAKVGDKT
  ELFKDLSDFPLIKKRKDEIQGVIDEIRMHLQEIRKILKNPSAQYVTVSGQEFMIEIKNSAVSCIPTDWVK
  VGSTKAVSRFHSPFIVENYRHLNQLREQLVLDCSAEWLDFLEKFSEHYHSLCKAVHHLATVDCIFSLAKV
  AKQGDYCRPTVQEERKIVIKNGRHPVIDVLLGEQDQYVPNNTDLSEDSERVMIITGPNMGGKSSYIKQVA
  LITIMAQIGSYVPAEEATIGIVDGIFTRMGAADNIYKGQSTFMEELTDTAEIIRKATSQSLVILDELGRG
  TSTHDGIAIAYATLEYFIRDVKSLTLFVTHYPPVCELEKNYSHQVGNYHMGFLVSEDESKLDPGAAEQVP
  DFVTFLYQITRGIAARSYGLNVAKLADVPGEILKKAAHKSKELEGLINTKRKRLKYFAKLWTMHNAQDLQ
  KWTEEFNMEETQTSLLH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002439
• ORF Size: 3411 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002439.5
• RefSeq Size: 4645 bp
• RefSeq ORF: 3414 bp
• Locus ID: 4437
• UniProt ID: P20585
• Cytogenetics: 5q14.1
• Domains: MutS_V, MutS_I, MutS_III, MutS_II
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Colorectal cancer, Mismatch repair, Pathways in cancer
• MW: 127.2 kDa
• Gene Summary: The protein encoded by this gene forms a heterodimer with MSH2 to form MutS beta, part of the post-replicative DNA mismatch repair system. MutS beta initiates mismatch repair by binding to a mismatch and then forming a complex with MutL alpha heterodimer. This gene contains a polymorphic 9 bp tandem repeat sequence in the first exon. The repeat is present 6 times in the reference genome sequence and 3-7 repeats have been reported. Defects in this gene are a cause of susceptibility to endometrial cancer. [provided by RefSeq, Mar 2011]