MSH3 (NM_002439) Human Tagged ORF Clone

CAT#: RC212391

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MSH3 (Myc-DDK-tagged)-Human mutS homolog 3 (E. coli) (MSH3)



  "NM_002439" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 997.00


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In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MSH3
Synonyms DUP; FAP4; MRP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC212391 representing NM_002439
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCGCCGGAAGCCTGCGTCGGGCGGCCTCGCTGCCTCCAGCTCAGCCCCTGCGAGGCAAGCGGTTT
TGAGCCGATTCTTCCAGTCTACGGGAAGCCTGAAATCCACCTCCTCCTCCACAGGTGCAGCCGACCAGGT
GGACCCTGGCGCTGCAGCGGCTGCAGCGGCCGCAGCGGCCGCAGCGCCCCCAGCGCCCCCAGCTCCCGCC
TTCCCGCCCCAGCTGCCGCCGCACATAGCTACAGAAATTGACAGAAGAAAGAAGAGACCATTGGAAAATG
ATGGGCCTGTTAAAAAGAAAGTAAAGAAAGTCCAACAAAAGGAAGGAGGAAGTGATCTGGGAATGTCTGG
CAACTCTGAGCCAAAGAAATGTCTGAGGACCAGGAATGTTTCAAAGTCTCTGGAAAAATTGAAAGAATTC
TGCTGCGATTCTGCCCTTCCTCAAAGTAGAGTCCAGACAGAATCTCTGCAGGAGAGATTTGCAGTTCTGC
CAAAATGTACTGATTTTGATGATATCAGTCTTCTACACGCAAAGAATGCAGTTTCTTCTGAAGATTCGAA
ACGTCAAATTAATCAAAAGGACACAACACTTTTTGATCTCAGTCAGTTTGGATCATCAAATACAAGTCAT
GAAAATTTACAGAAAACTGCTTCCAAATCAGCTAACAAACGGTCCAAAAGCATCTATACGCCGCTAGAAT
TACAATACATAGAAATGAAGCAGCAGCACAAAGATGCAGTTTTGTGTGTGGAATGTGGATATAAGTATAG
ATTCTTTGGGGAAGATGCAGAGATTGCAGCCCGAGAGCTCAATATTTATTGCCATTTAGATCACAACTTT
ATGACAGCAAGTATACCTACTCACAGACTGTTTGTTCATGTACGCCGCCTGGTGGCAAAAGGATATAAGG
TGGGAGTTGTGAAGCAAACTGAAACTGCAGCATTAAAGGCCATTGGAGACAACAGAAGTTCACTCTTTTC
CCGGAAATTGACTGCCCTTTATACAAAATCTACACTTATTGGAGAAGATGTGAATCCCCTAATCAAGCTG
GATGATGCTGTAAATGTTGATGAGATAATGACTGATACTTCTACCAGCTATCTTCTGTGCATCTCTGAAA
ATAAGGAAAATGTTAGGGACAAAAAAAAGGGCAACATTTTTATTGGCATTGTGGGAGTGCAGCCTGCCAC
AGGCGAGGTTGTGTTTGATAGTTTCCAGGACTCTGCTTCTCGTTCAGAGCTAGAAACCCGGATGTCAAGC
CTGCAGCCAGTAGAGCTGCTGCTTCCTTCGGCCTTGTCCGAGCAAACAGAGGCGCTCATCCACAGAGCCA
CATCTGTTAGTGTGCAGGATGACAGAATTCGAGTCGAAAGGATGGATAACATTTATTTTGAATACAGCCA
TGCTTTCCAGGCAGTTACAGAGTTTTATGCAAAAGATACAGTTGACATCAAAGGTTCTCAAATTATTTCT
GGCATTGTTAACTTAGAGAAGCCTGTGATTTGCTCTTTGGCTGCCATCATAAAATACCTCAAAGAATTCA
ACTTGGAAAAGATGCTCTCCAAACCTGAGAATTTTAAACAGCTATCAAGTAAAATGGAATTTATGACAAT
TAATGGAACAACATTAAGGAATCTGGAAATCCTACAGAATCAGACTGATATGAAAACCAAAGGAAGTTTG
CTGTGGGTTTTAGACCACACTAAAACTTCATTTGGGAGACGGAAGTTAAAGAAGTGGGTGACCCAGCCAC
TCCTTAAATTAAGGGAAATAAATGCCCGGCTTGATGCTGTATCGGAAGTTCTCCATTCAGAATCTAGTGT
GTTTGGTCAGATAGAAAATCATCTACGTAAATTGCCCGACATAGAGAGGGGACTCTGTAGCATTTATCAC
AAAAAATGTTCTACCCAAGAGTTCTTCTTGATTGTCAAAACTTTATATCACCTAAAGTCAGAATTTCAAG
CAATAATACCTGCTGTTAATTCCCACATTCAGTCAGACTTGCTCCGGACCGTTATTTTAGAAATTCCTGA
ACTCCTCAGTCCAGTGGAGCATTACTTAAAGATACTCAATGAACAAGCTGCCAAAGTTGGGGATAAAACT
GAATTATTTAAAGACCTTTCTGACTTCCCTTTAATAAAAAAGAGGAAGGATGAAATTCAAGGTGTTATTG
ACGAGATCCGAATGCATTTGCAAGAAATACGAAAAATACTAAAAAATCCTTCTGCACAATATGTGACAGT
ATCAGGACAGGAGTTTATGATAGAAATAAAGAACTCTGCTGTATCTTGTATACCAACTGATTGGGTAAAG
GTTGGAAGCACAAAAGCTGTGAGCCGCTTTCACTCTCCTTTTATTGTAGAAAATTACAGACATCTGAATC
AGCTCCGGGAGCAGCTAGTCCTTGACTGCAGTGCTGAATGGCTTGATTTTCTAGAGAAATTCAGTGAACA
TTATCACTCCTTGTGTAAAGCAGTGCATCACCTAGCAACTGTTGACTGCATTTTCTCCCTGGCCAAGGTC
GCTAAGCAAGGAGATTACTGCAGACCAACTGTACAAGAAGAAAGAAAAATTGTAATAAAAAATGGAAGGC
ACCCTGTGATTGATGTGTTGCTGGGAGAACAGGATCAATATGTCCCAAATAATACAGATTTATCAGAGGA
CTCAGAGAGAGTAATGATAATTACCGGACCAAACATGGGTGGAAAGAGCTCCTACATAAAACAAGTTGCA
TTGATTACCATCATGGCTCAGATTGGCTCCTATGTTCCTGCAGAAGAAGCGACAATTGGGATTGTGGATG
GCATTTTCACAAGGATGGGTGCTGCAGACAATATATATAAAGGACAGAGTACATTTATGGAAGAACTGAC
TGACACAGCAGAAATAATCAGAAAAGCAACATCACAGTCCTTGGTTATCTTGGATGAACTAGGAAGAGGG
ACGAGCACTCATGATGGAATTGCCATTGCCTATGCTACACTTGAGTATTTCATCAGAGATGTGAAATCCT
TAACCCTGTTTGTCACCCATTATCCGCCAGTTTGTGAACTAGAAAAAAATTACTCACACCAGGTGGGGAA
TTACCACATGGGATTCTTGGTCAGTGAGGATGAAAGCAAACTGGATCCAGGCGCAGCAGAACAAGTCCCT
GATTTTGTCACCTTCCTTTACCAAATAACTAGAGGAATTGCAGCAAGGAGTTATGGATTAAATGTGGCTA
AACTAGCAGATGTTCCTGGAGAAATTTTGAAGAAAGCAGCTCACAAGTCAAAAGAGCTGGAAGGATTAAT
AAATACGAAAAGAAAGAGACTCAAGTATTTTGCAAAGTTATGGACGATGCATAATGCACAAGACCTGCAG
AAGTGGACAGAGGAGTTCAACATGGAAGAAACACAGACTTCTCTTCTTCAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC212391 representing NM_002439
Red=Cloning site Green=Tags(s)

MSRRKPASGGLAASSSAPARQAVLSRFFQSTGSLKSTSSSTGAADQVDPGAAAAAAAAAAAAPPAPPAPA
FPPQLPPHIATEIDRRKKRPLENDGPVKKKVKKVQQKEGGSDLGMSGNSEPKKCLRTRNVSKSLEKLKEF
CCDSALPQSRVQTESLQERFAVLPKCTDFDDISLLHAKNAVSSEDSKRQINQKDTTLFDLSQFGSSNTSH
ENLQKTASKSANKRSKSIYTPLELQYIEMKQQHKDAVLCVECGYKYRFFGEDAEIAARELNIYCHLDHNF
MTASIPTHRLFVHVRRLVAKGYKVGVVKQTETAALKAIGDNRSSLFSRKLTALYTKSTLIGEDVNPLIKL
DDAVNVDEIMTDTSTSYLLCISENKENVRDKKKGNIFIGIVGVQPATGEVVFDSFQDSASRSELETRMSS
LQPVELLLPSALSEQTEALIHRATSVSVQDDRIRVERMDNIYFEYSHAFQAVTEFYAKDTVDIKGSQIIS
GIVNLEKPVICSLAAIIKYLKEFNLEKMLSKPENFKQLSSKMEFMTINGTTLRNLEILQNQTDMKTKGSL
LWVLDHTKTSFGRRKLKKWVTQPLLKLREINARLDAVSEVLHSESSVFGQIENHLRKLPDIERGLCSIYH
KKCSTQEFFLIVKTLYHLKSEFQAIIPAVNSHIQSDLLRTVILEIPELLSPVEHYLKILNEQAAKVGDKT
ELFKDLSDFPLIKKRKDEIQGVIDEIRMHLQEIRKILKNPSAQYVTVSGQEFMIEIKNSAVSCIPTDWVK
VGSTKAVSRFHSPFIVENYRHLNQLREQLVLDCSAEWLDFLEKFSEHYHSLCKAVHHLATVDCIFSLAKV
AKQGDYCRPTVQEERKIVIKNGRHPVIDVLLGEQDQYVPNNTDLSEDSERVMIITGPNMGGKSSYIKQVA
LITIMAQIGSYVPAEEATIGIVDGIFTRMGAADNIYKGQSTFMEELTDTAEIIRKATSQSLVILDELGRG
TSTHDGIAIAYATLEYFIRDVKSLTLFVTHYPPVCELEKNYSHQVGNYHMGFLVSEDESKLDPGAAEQVP
DFVTFLYQITRGIAARSYGLNVAKLADVPGEILKKAAHKSKELEGLINTKRKRLKYFAKLWTMHNAQDLQ
KWTEEFNMEETQTSLLH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002439
ORF Size 3411 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002439.5
RefSeq Size 4645 bp
RefSeq ORF 3414 bp
Locus ID 4437
UniProt ID P20585
Cytogenetics 5q14.1
Domains MutS_V, MutS_I, MutS_III, MutS_II
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Colorectal cancer, Mismatch repair, Pathways in cancer
MW 127.2 kDa
Gene Summary The protein encoded by this gene forms a heterodimer with MSH2 to form MutS beta, part of the post-replicative DNA mismatch repair system. MutS beta initiates mismatch repair by binding to a mismatch and then forming a complex with MutL alpha heterodimer. This gene contains a polymorphic 9 bp tandem repeat sequence in the first exon. The repeat is present 6 times in the reference genome sequence and 3-7 repeats have been reported. Defects in this gene are a cause of susceptibility to endometrial cancer. [provided by RefSeq, Mar 2011]

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