MADCAM1 (NM_130762) Human Tagged ORF Clone

CAT#: RC212381

MADCAM1 (Myc-DDK-tagged)-Human mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 2

Product Images

Circular map for RC212381

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  Coomassie blue staining of purified MADCAM1 protein (Cat# TP312381). The protein was produced from HEK293T cells transfected with MADCAM1 cDNA clone (Cat# RC212381) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MADCAM1
• Synonyms: MACAM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212381 representing NM_130762
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTCGGACTGGCCCTCCTGCTGGCGGGGCTTCTGGGGCTCCTCCTCGGCCAGTCCCTCCAGGTGA
  AGCCCCTGCAGGTGGAGCCCCCGGAGCCGGTGGTGGCCGTGGCCTTGGGCGCCTCGCGCCAGCTCACCTG
  CCGCCTGGCCTGCGCGGACCGCGGGGCCTCGGTGCAGTGGCGGGGCCTGGACACCAGCCTGGGCGCGGTG
  CAGTCGGACACGGGCCGCAGCGTCCTCACCGTGCGCAACGCCTCGCTGTCGGCGGCCGGGACCCGCGTGT
  GCGTGGGCTCCTGCGGGGGCCGCACCTTCCAGCACACCGTGCAGCTCCTTGTGTACGCCTTCCCGGACCA
  GCTGACCGTCTCCCCAGCAGCCCTGGTGCCTGGTGACCCGGAGGTGGCCTGTACGGCCCACAAAGTCACG
  CCCGTGGACCCCAACGCGCTCTCCTTCTCCCTGCTCGTCGGGGGCCAGGAACTGGAGGGGGCGCAAGCCC
  TGGGCCCGGAGGTGCAGGAGGAGGAGGAGGAGCCCCAGGGGGACGAGGACGTGCTGTTCAGGGTGACAGA
  GCGCTGGCGGCTGCCGCCCCTGGGGACCCCTGTCCCGCCCGCCCTCTACTGCCAGGCCACGATGAGGCTG
  CCTGGCTTGGAGCTCAGCCACCGCCAGGCCATCCCCGCGTCCAAACCTGCGGGTGACCAGCTGCCCGCGG
  CTCTGTGGACCAGCAGTGCGGTGCTGGGACTGCTGCTCCTGGCCTTGCCCACCTATCACCTCTGGAAACG
  CTGCCGGCACCTGGCTGAGGACGACACCCACCCACCAGCTTCTCTGAGGCTTCTGCCCCAGGTGTCGGCC
  TGGGCTGGGTTAAGGGGGACCGGCCAGGTCGGGATCAGCCCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212381 representing NM_130762
  Red=Cloning site Green=Tags(s)
  
  MDFGLALLLAGLLGLLLGQSLQVKPLQVEPPEPVVAVALGASRQLTCRLACADRGASVQWRGLDTSLGAV
  QSDTGRSVLTVRNASLSAAGTRVCVGSCGGRTFQHTVQLLVYAFPDQLTVSPAALVPGDPEVACTAHKVT
  PVDPNALSFSLLVGGQELEGAQALGPEVQEEEEEPQGDEDVLFRVTERWRLPPLGTPVPPALYCQATMRL
  PGLELSHRQAIPASKPAGDQLPAALWTSSAVLGLLLLALPTYHLWKRCRHLAEDDTHPPASLRLLPQVSA
  WAGLRGTGQVGISPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130762
• ORF Size: 885 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130762.3
• RefSeq Size: 1275 bp
• RefSeq ORF: 888 bp
• Locus ID: 8174
• UniProt ID: Q13477
• Cytogenetics: 19p13.3
• Protein Pathways: Cell adhesion molecules (CAMs)
• MW: 29.3 kDa
• Gene Summary: The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]