TAF4B (NM_005640) Human Tagged ORF Clone

CAT#: RC212361

TAF4B (Myc-DDK-tagged)-Human TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa (TAF4B)

Product Images

Circular map for RC212361

• 

  Western blot validation of overexpression lysate (Cat# LY417171) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212361 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAF4B
• Synonyms: SPGF13; TAF2C2; TAFII105
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212361 representing NM_005640
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGCCGGCCTCACCGAACCCGCCGGCGCCGCTCCCCCGGCTGCTGTGAGCGCCTCGGGGACCGTGA
  CCATGGCCCCGGCCGGGGCGCTGCCGGTGCGGGTGGAGAGCACTCCGGTGGCCCTGGGCGCCGTGACTAA
  GGCTCCTGTCAGCGTCTGCGTGGAGCCCACGGCGTCCCAGCCCCTGCGGTCCCCCGTGGGGACCCTGGTG
  ACCAAAGTGGCTCCGGTCAGCGCCCCTCCTAAAGTCAGCAGCGGCCCTAGGCTGCCTGCTCCTCAGATAG
  TCGCCGTGAAAGCCCCCAACACCACGACAATCCAGTTTCCTGCTAATTTGCAGCTTCCTCCAGGAACCGT
  TTTGATTAAAAGTAACAGTGGTCCGTTGATGTTGGTATCTCCTCAGCAAACTGTAACAAGAGCCGAGACC
  ACAAGTAACATAACCTCAAGGCCAGCAGTACCAGCGAATCCTCAAACAGTCAAAATCTGTACAGTGCCGA
  ACTCTAGCTCACAATTAATCAAGAAAGTGGCAGTGACACCTGTTAAAAAATTGGCACAAATAGGAACTAC
  TGTGGTAACCACTGTTCCGAAGCCTTCCTCAGTACAATCTGTGGCTGTGCCAACCAGTGTCGTCACAGTT
  ACTCCTGGAAAGCCATTGAATACTGTAACTACCCTGAAGCCTTCAAGTTTGGGAGCATCATCCACTCCTT
  CAAATGAGCCCAATCTTAAAGCAGAGAACTCAGCAGCTGTTCAGATTAATCTTTCTCCGACAATGCTAGA
  AAATGTGAAGAAATGCAAGAACTTCCTTGCAATGTTAATAAAACTAGCATGTAGTGGATCACAGTCCCCA
  GAAATGGGGCAAAATGTGAAGAAGCTGGTGGAACAACTTTTGGATGCAAAAATCGAAGCAGAAGAATTTA
  CTAGGAAACTGTATGTTGAACTCAAGTCTTCACCTCAGCCTCACCTGGTTCCTTTTCTTAAGAAAAGCGT
  GGTTGCCTTACGACAACTTCTGCCTAACTCCCAGAGCTTCATCCAGCAATGTGTTCAGCAGACTTCTAGT
  GACATGGTCATTGCTACCTGTACTACAACAGTAACAACTTCTCCTGTGGTGACAACTACAGTGTCCTCAA
  GCCAGTCTGAAAAGTCAATTATTGTTTCTGGAGCAACAGCACCCAGAACTGTGTCAGTGCAAACTTTGAA
  CCCACTTGCTGGTCCAGTGGGAGCAAAAGCTGGAGTTGTGACACTTCATTCTGTGGGCCCAACTGCTGCA
  ACAGGAGGAACAACAGCTGGAACTGGTTTGCTTCAGACTTCAAAACCACTTGTGACATCTGTGGCAAACA
  CAGTGACCACGGTCTCACTGCAACCTGAAAAGCCAGTTGTCTCTGGAACAGCAGTAACACTGTCCCTTCC
  AGCAGTAACTTTTGGAGAAACTTCAGGTGCAGCTATTTGTCTTCCATCTGTGAAACCTGTTGTTTCTTCT
  GCTGGGACCACATCTGACAAGCCTGTTATTGGGACTCCAGTTCAAATCAAACTTGCCCAGCCGGGCCCTG
  TCCTTTCACAACCAGCTGGGATTCCACAGGCAGTTCAAGTCAAGCAACTAGTAGTTCAGCAGCCTTCAGG
  AGGCAATGAAAAACAAGTGACCACAATTTCACATTCCTCAACATTGACCATTCAGAAATGTGGACAGAAG
  ACGATGCCAGTGAACACCATAATACCTACTAGTCAGTTTCCTCCAGCTTCCATTCTAAAGCAAATTACTC
  TGCCTGGAAATAAAATTCTGTCACTTCAAGCATCTCCTACTCAGAAAAATAGAATAAAAGAGAATGTAAC
  ATCATGCTTCCGAGATGAGGATGACATCAATGATGTGACTTCTATGGCAGGGGTCAACCTTAATGAAGAA
  AATGCCTGCATCTTAGCAACAAACTCTGAATTGGTTGGCACACTCATTCAGTCATGTAAAGATGAACCAT
  TTCTTTTTATTGGAGCTCTACAAAAGAGAATTTTAGACATTGGTAAAAAGCATGACATTACAGAACTTAA
  CTCTGATGCTGTGAACTTGATCTCCCAAGCAACACAGGAACGACTACGAGGCCTTCTAGAAAAACTGACT
  GCAATTGCTCAGCATCGAATGACTACTTACAAGGCAAGTGAAAATTACATCCTGTGTAGTGATACCAGGT
  CACAGCTCAAATTTCTTGAAAAGCTGGATCAATTGGAGAAGCAGAGAAAGGATTTGGAAGAAAGAGAAAT
  GTTACTTAAGGCAGCCAAGAGTCGTTCTAATAAAGAAGATCCAGAACAGCTGAGATTAAAGCAGAAAGCC
  AAAGAGTTACAGCAATTGGAACTTGCACAGATACAGCATAGAGACGCTAATCTCACAGCTCTTGCAGCTA
  TTGGACCAAGGAAGAAGAGACCACTAGAATCTGGAATTGAGGGCTTAAAAGACAACCTTCTTGCTTCTGG
  GACATCCAGCCTGACAGCCACCAAACAGTTGCATCGTCCAAGAATCACGAGAATCTGCCTCAGGGACTTG
  ATATTTTGTATGGAACAGGAACGGGAGATGAAGTATTCTCGAGCTCTATACCTGGCCCTTCTGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212361 representing NM_005640
  Red=Cloning site Green=Tags(s)
  
  MPAGLTEPAGAAPPAAVSASGTVTMAPAGALPVRVESTPVALGAVTKAPVSVCVEPTASQPLRSPVGTLV
  TKVAPVSAPPKVSSGPRLPAPQIVAVKAPNTTTIQFPANLQLPPGTVLIKSNSGPLMLVSPQQTVTRAET
  TSNITSRPAVPANPQTVKICTVPNSSSQLIKKVAVTPVKKLAQIGTTVVTTVPKPSSVQSVAVPTSVVTV
  TPGKPLNTVTTLKPSSLGASSTPSNEPNLKAENSAAVQINLSPTMLENVKKCKNFLAMLIKLACSGSQSP
  EMGQNVKKLVEQLLDAKIEAEEFTRKLYVELKSSPQPHLVPFLKKSVVALRQLLPNSQSFIQQCVQQTSS
  DMVIATCTTTVTTSPVVTTTVSSSQSEKSIIVSGATAPRTVSVQTLNPLAGPVGAKAGVVTLHSVGPTAA
  TGGTTAGTGLLQTSKPLVTSVANTVTTVSLQPEKPVVSGTAVTLSLPAVTFGETSGAAICLPSVKPVVSS
  AGTTSDKPVIGTPVQIKLAQPGPVLSQPAGIPQAVQVKQLVVQQPSGGNEKQVTTISHSSTLTIQKCGQK
  TMPVNTIIPTSQFPPASILKQITLPGNKILSLQASPTQKNRIKENVTSCFRDEDDINDVTSMAGVNLNEE
  NACILATNSELVGTLIQSCKDEPFLFIGALQKRILDIGKKHDITELNSDAVNLISQATQERLRGLLEKLT
  AIAQHRMTTYKASENYILCSDTRSQLKFLEKLDQLEKQRKDLEEREMLLKAAKSRSNKEDPEQLRLKQKA
  KELQQLELAQIQHRDANLTALAAIGPRKKRPLESGIEGLKDNLLASGTSSLTATKQLHRPRITRICLRDL
  IFCMEQEREMKYSRALYLALLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005640
• ORF Size: 2586 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005640.3
• RefSeq Size: 4777 bp
• RefSeq ORF: 2589 bp
• Locus ID: 6875
• UniProt ID: Q92750
• Cytogenetics: 18q11.2
• Protein Pathways: Basal transcription factors, Huntington's disease
• MW: 90.9 kDa
• Gene Summary: TATA binding protein (TBP) and TBP-associated factors (TAFs) participate in the formation of the TFIID protein complex, which is involved in initiation of transcription of genes by RNA polymerase II. This gene encodes a cell type-specific TAF that may be responsible for mediating transcription by a subset of activators in B cells. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]