TZFP (ZBTB32) (NM_014383) Human Tagged ORF Clone

CAT#: RC212308

ZBTB32 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 32 (ZBTB32)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_014383" in other vectors (4)

Reconstitution Protocol

USD 686.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

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ZBTB32 mouse monoclonal antibody,clone OTI2H8
    • 100 ul

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Other products for "TZFP"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TZFP
Synonyms FAXF; FAZF; Rog; TZFP; ZNF538
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC212308 representing NM_014383
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCTGCCCCCCATAAGACTGCCCAGCCCCTATGGCTCTGATCGGCTGGTACAGCTAGCAGCCAGGC
TCCGGCCAGCACTCTGTGATACTCTGATCACCGTAGGGAGCCAGGAGTTCCCCGCCCACAGCCTGGTGCT
AGCAGGTGTCAGCCAGCAGCTGGGCCGCAGGGGCCAGTGGGCTCTGGGAGAAGGCATCAGCCCTTCTACC
TTTGCCCAGCTCCTGAACTTTGTGTATGGGGAGAGTGTAGAGCTGCAGCCTGGAGAGCTAAGGCCCCTTC
AGGAGGCGGCCAGGGCCTTGGGAGTGCAGTCCCTGGAAGAGGCATGCTGGAGGGCTCGAGGGGACAGGGC
TAAAAAGCCAGATCCAGGCCTGAAGAAACATCAGGAGGAGCCAGAGAAACCCTCAAGGAATCCTGAGAGA
GAACTGGGGGACCCTGGAGAGAAGCAGAAACCAGAACAGGTTTCTAGAACTGGTGGGAGAGAACAGGAGA
TGTTGCACAAGCACTCGCCACCAAGAGGCAGACCCGAGATGGCAGGAGCAACGCAGGAGGCTCAGCAGGA
ACAGACCAGGTCAAAGGAGAAACGCCTCCAAGCCCCTGTTGGCCAAAGGGGAGCAGATGGGAAGCATGGA
GTGCTCACGTGGTTGAGGGAAAATCCAGGGGGCTCTGAGGAAAGTCTGCGCAAGCTCCCTGGCCCCCTTC
CCCCAGCAGGCTCCCTGCAAACCAGCGTCACCCCTAGGCCCTCGTGGGCTGAGGCCCCTTGGTTGGTGGG
GGGCCAGCCTGCCCTGTGGAGCATCCTGCTGATGCCGCCCAGATATGGCATTCCCTTCTACCATAGCACC
CCCACCACTGGAGCCTGGCAGGAGGTCTGGCGGGAACAGAGGATCCCACTGTCCCTAAATGCCCCCAAAG
GGCTCTGGAGCCAGAACCAGTTGGCCTCCTCCAGCCCTACCCCAGGTTCCCTCCCCCAGGGCCCCGCACA
GCTCAGCCCTGGGGAGATGGAAGAGTCTGATCAGGGGCACACAGGCGCACTTGCAACCTGTGCGGGTCAT
GAGGACAAGGCAGGCTGCCCACCTCGCCCGCACCCTCCCCCGGCCCCTCCTGCTCGGTCTCGGCCCTATG
CGTGCTCTGTCTGTGGAAAGAGGTTTTCACTCAAGCATCAGATGGAGACGCACTACCGAGTCCACACAGG
AGAGAAGCCCTTCTCCTGTAGCCTTTGTCCTCAGCGCTCCCGGGACTTCTCGGCCATGACCAAGCACCTG
CGGACACACGGGGCCGCTCCGTACCGCTGCTCCCTGTGCGGGGCCGGCTGTCCCAGCCTGGCCTCCATGC
AGGCGCACATGCGCGGTCACTCGCCCAGCCAACTCCCGCCCGGATGGACCATCCGCTCCACCTTCCTCTA
CTCCTCCTCGAGGCCGTCTCGGCCCTCGACCTCTCCCTGTTGTCCTTCTTCCTCCACCACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC212308 representing NM_014383
Red=Cloning site Green=Tags(s)

MSLPPIRLPSPYGSDRLVQLAARLRPALCDTLITVGSQEFPAHSLVLAGVSQQLGRRGQWALGEGISPST
FAQLLNFVYGESVELQPGELRPLQEAARALGVQSLEEACWRARGDRAKKPDPGLKKHQEEPEKPSRNPER
ELGDPGEKQKPEQVSRTGGREQEMLHKHSPPRGRPEMAGATQEAQQEQTRSKEKRLQAPVGQRGADGKHG
VLTWLRENPGGSEESLRKLPGPLPPAGSLQTSVTPRPSWAEAPWLVGGQPALWSILLMPPRYGIPFYHST
PTTGAWQEVWREQRIPLSLNAPKGLWSQNQLASSSPTPGSLPQGPAQLSPGEMEESDQGHTGALATCAGH
EDKAGCPPRPHPPPAPPARSRPYACSVCGKRFSLKHQMETHYRVHTGEKPFSCSLCPQRSRDFSAMTKHL
RTHGAAPYRCSLCGAGCPSLASMQAHMRGHSPSQLPPGWTIRSTFLYSSSRPSRPSTSPCCPSSSTT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014383
ORF Size 1461 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014383.3
RefSeq Size 1960 bp
RefSeq ORF 1464 bp
Locus ID 27033
UniProt ID Q9Y2Y4
Cytogenetics 19q13.12
Protein Families Transcription Factors
MW 53.4 kDa
Gene Summary DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T-cells (By similarity).[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.