AP2 alpha (AP2A1) (NM_130787) Human Tagged ORF Clone

CAT#: RC212287

AP2A1 (Myc-DDK-tagged)-Human adaptor-related protein complex 2, alpha 1 subunit (AP2A1), transcript variant 2

Product Images

Circular map for RC212287

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AP2 alpha
• Synonyms: ADTAA; AP2-ALPHA; CLAPA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212287 representing NM_130787
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCCGTGTCCAAGGGCGATGGGATGCGGGGGCTCGCGGTGTTCATCTCCGACATCCGGAACTGTA
  AGAGCAAAGAGGCGGAAATTAAGAGAATCAACAAGGAACTGGCCAACATCCGCTCCAAGTTCAAAGGAGA
  CAAAGCCTTGGATGGCTACAGTAAGAAAAAATATGTGTGTAAACTGCTTTTCATCTTCCTGCTTGGCCAT
  GACATTGACTTTGGGCACATGGAGGCTGTGAATCTGTTGAGTTCCAATAAATACACAGAGAAGCAAATAG
  GTTACCTGTTCATTTCTGTGCTGGTGAACTCGAACTCGGAGCTGATCCGCCTCATCAACAACGCCATCAA
  GAATGACCTGGCCAGCCGCAACCCCACCTTCATGTGCCTGGCCCTGCACTGCATCGCCAACGTGGGCAGC
  CGGGAGATGGGCGAGGCCTTTGCCGCTGACATCCCCCGCATCCTGGTGGCCGGGGACAGCATGGACAGTG
  TCAAGCAGAGTGCGGCCCTGTGCCTCCTTCGACTGTACAAGGCCTCGCCTGACCTGGTGCCCATGGGCGA
  GTGGACGGCGCGTGTGGTACACCTGCTCAATGACCAGCACATGGGTGTGGTCACGGCCGCCGTCAGCCTC
  ATCACCTGTCTCTGCAAGAAGAACCCAGATGACTTCAAGACGTGCGTCTCTCTGGCTGTGTCGCGCCTGA
  GCCGGATCGTCTCCTCTGCCTCCACCGACCTCCAGGACTACACCTACTACTTCGTCCCAGCACCCTGGCT
  CTCGGTGAAGCTCCTGCGGCTGCTGCAGTGCTACCCGCCTCCAGAGGATGCGGCTGTGAAGGGGCGGCTG
  GTGGAATGTCTGGAGACTGTGCTCAACAAGGCCCAGGAGCCCCCCAAATCCAAGAAGGTGCAGCATTCCA
  ACGCCAAGAACGCCATCCTCTTCGAGACCATCAGCCTCATCATCCACTATGACAGTGAGCCCAACCTCCT
  GGTTCGGGCCTGCAACCAGCTGGGCCAGTTCCTGCAGCACCGGGAGACCAACCTGCGCTACCTGGCCCTG
  GAGAGCATGTGCACGCTGGCCAGCTCCGAGTTCTCCCATGAAGCCGTCAAGACGCACATTGACACCGTCA
  TCAATGCCCTCAAGACGGAGCGGGACGTCAGCGTGCGGCAGCGGGCGGCTGACCTCCTCTACGCCATGTG
  TGACCGGAGCAATGCCAAGCAGATCGTGTCGGAGATGCTGCGGTACCTGGAGACGGCAGACTACGCCATC
  CGCGAGGAGATCGTCCTGAAGGTGGCCATCCTGGCCGAGAAGTACGCCGTGGACTACAGCTGGTACGTGG
  ACACCATCCTCAACCTCATCCGCATTGCGGGCGACTACGTGAGTGAGGAGGTGTGGTACCGTGTGCTACA
  GATCGTCACCAACCGTGATGACGTCCAGGGCTATGCCGCCAAGACCGTCTTTGAGGCGCTCCAGGCCCCT
  GCCTGTCACGAGAACATGGTGAAGGTTGGCGGCTACATCCTTGGGGAGTTTGGGAACCTGATTGCTGGGG
  ACCCCCGCTCCAGCCCCCCAGTGCAGTTCTCCCTGCTCCACTCCAAGTTCCATCTGTGCAGCGTGGCCAC
  GCGGGCGCTGCTGCTGTCCACCTACATCAAGTTCATCAACCTCTTCCCCGAGACCAAGGCCACCATCCAG
  GGCGTCCTGCGGGCCGGCTCCCAGCTGCGCAATGCTGACGTGGAGCTGCAGCAGCGAGCCGTGGAGTACC
  TCACCCTCAGCTCAGTGGCCAGCACCGACGTCCTGGCCACGGTGCTGGAGGAGATGCCGCCCTTCCCCGA
  GCGCGAGTCGTCCATCCTGGCCAAGCTGAAACGCAAGAAGGGGCCAGGGGCCGGCAGCGCCCTGGACGAT
  GGCCGGAGGGACCCCAGCAGCAACGACATCAACGGGGGCATGGAGCCCACCCCCAGCACTGTGTCGACGC
  CCTCGCCCTCCGCCGACCTCCTGGGGCTGCGGGCAGCCCCTCCCCCGGCAGCACCCCCGGCTTCTGCAGG
  AGCAGGGAACCTTCTGGTGGACGTCTTCGATGGCCCGGCCGCCCAGCCCAGCCTGGGGCCCACCCCCGAG
  GAGGCCTTCCTCAGCCCAGGTCCTGAGGACATCGGCCCTCCCATTCCGGAAGCCGATGAGTTGCTGAATA
  AGTTTGTGTGTAAGAACAACGGGGTCCTGTTCGAGAACCAGCTGCTGCAGATCGGAGTCAAGTCAGAGTT
  CCGACAGAACCTGGGCCGCATGTATCTCTTCTATGGCAACAAGACCTCGGTGCAGTTCCAGAATTTCTCA
  CCCACTGTGGTTCACCCGGGAGACCTCCAGACTCAGCTGGCTGTGCAGACCAAGCGCGTGGCGGCGCAGG
  TGGACGGCGGCGCGCAGGTGCAGCAGGTGCTCAATATCGAGTGCCTGCGGGACTTCCTGACGCCCCCGCT
  GCTGTCCGTGCGCTTCCGGTACGGTGGCGCCCCCCAGGCCCTCACCCTGAAGCTCCCAGTGACCATCAAC
  AAGTTCTTCCAGCCCACCGAGATGGCGGCCCAGGATTTCTTCCAGCGCTGGAAGCAGCTGAGCCTCCCTC
  AACAGGAGGCGCAGAAAATCTTCAAAGCCAACCACCCCATGGACGCAGAAGTTACTAAGGCCAAGCTTCT
  GGGGTTTGGCTCTGCTCTCCTGGACAATGTGGACCCCAACCCTGAGAACTTCGTGGGGGCGGGGATCATC
  CAGACTAAAGCCCTGCAGGTGGGCTGTCTGCTTCGGCTGGAGCCCAATGCCCAGGCCCAGATGTACCGGC
  TGACCCTGCGCACCAGCAAGGAGCCCGTCTCCCGTCACCTGTGTGAGCTGCTGGCACAGCAGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212287 representing NM_130787
  Red=Cloning site Green=Tags(s)
  
  MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGH
  DIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGS
  REMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSL
  ITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRL
  VECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLAL
  ESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAI
  REEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAP
  ACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQ
  GVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAGSALDD
  GRRDPSSNDINGGMEPTPSTVSTPSPSADLLGLRAAPPPAAPPASAGAGNLLVDVFDGPAAQPSLGPTPE
  EAFLSPGPEDIGPPIPEADELLNKFVCKNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFS
  PTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQALTLKLPVTIN
  KFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGII
  QTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130787
• ORF Size: 2865 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130787.3
• RefSeq Size: 3433 bp
• RefSeq ORF: 2868 bp
• Locus ID: 160
• UniProt ID: O95782
• Cytogenetics: 19q13.33
• Protein Pathways: Endocytosis, Huntington's disease
• MW: 105.2 kDa
• Gene Summary: This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]