PGC1 alpha (PPARGC1A) (NM_013261) Human Tagged ORF Clone

CAT#: RC212244

PPARGC1A (Myc-DDK-tagged)-Human peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (PPARGC1A)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPARGC1A
• Synonyms: LEM6; PGC-1(alpha); PGC-1alpha; PGC-1v; PGC1; PGC1A; PPARGC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212244 representing NM_013261
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTGGGACATGTGCAACCAGGACTCTGAGTCTGTATGGAGTGACATCGAGTGTGCTGCTCTGGTTG
  GTGAAGACCAGCCTCTTTGCCCAGATCTTCCTGAACTTGATCTTTCTGAACTAGATGTGAACGACTTGGA
  TACAGACAGCTTTCTGGGTGGACTCAAGTGGTGCAGTGACCAATCAGAAATAATATCCAATCAGTACAAC
  AATGAGCCTTCAAACATATTTGAGAAGATAGATGAAGAGAATGAGGCAAACTTGCTAGCAGTCCTCACAG
  AGACACTAGACAGTCTCCCTGTGGATGAAGACGGATTGCCCTCATTTGATGCGCTGACAGATGGAGACGT
  GACCACTGACAATGAGGCTAGTCCTTCCTCCATGCCTGACGGCACCCCTCCACCCCAGGAGGCAGAAGAG
  CCGTCTCTACTTAAGAAGCTCTTACTGGCACCAGCCAACACTCAGCTAAGTTATAATGAATGCAGTGGTC
  TCAGTACCCAGAACCATGCAAATCACAATCACAGGATCAGAACAAACCCTGCAATTGTTAAGACTGAGAA
  TTCATGGAGCAATAAAGCGAAGAGTATTTGTCAACAGCAAAAGCCACAAAGACGTCCCTGCTCGGAGCTT
  CTCAAATATCTGACCACAAACGATGACCCTCCTCACACCAAACCCACAGAGAACAGAAACAGCAGCAGAG
  ACAAATGCACCTCCAAAAAGAAGTCCCACACACAGTCGCAGTCACAACACTTACAAGCCAAACCAACAAC
  TTTATCTCTTCCTCTGACCCCAGAGTCACCAAATGACCCCAAGGGTTCCCCATTTGAGAACAAGACTATT
  GAACGCACCTTAAGTGTGGAACTCTCTGGAACTGCAGGCCTAACTCCACCCACCACTCCTCCTCATAAAG
  CCAACCAAGATAACCCTTTTAGGGCTTCTCCAAAGCTGAAGTCCTCTTGCAAGACTGTGGTGCCACCACC
  ATCAAAGAAGCCCAGGTACAGTGAGTCTTCTGGTACACAAGGCAATAACTCCACCAAGAAAGGGCCGGAG
  CAATCCGAGTTGTATGCACAACTCAGCAAGTCCTCAGTCCTCACTGGTGGACACGAGGAAAGGAAGACCA
  AGCGGCCCAGTCTGCGGCTGTTTGGTGACCATGACTATTGCCAGTCAATTAATTCCAAAACGGAAATACT
  CATTAATATATCACAGGAGCTCCAAGACTCTAGACAACTAGAAAATAAAGATGTCTCCTCTGATTGGCAG
  GGGCAGATTTGTTCTTCCACAGATTCAGACCAGTGCTACCTGAGAGAGACTTTGGAGGCAAGCAAGCAGG
  TCTCTCCTTGCAGCACAAGAAAACAGCTCCAAGACCAGGAAATCCGAGCCGAGCTGAACAAGCACTTCGG
  TCATCCCAGTCAAGCTGTTTTTGACGACGAAGCAGACAAGACCAGTGAACTGAGGGACAGTGATTTCAGT
  AATGAACAATTCTCCAAACTACCTATGTTTATAAATTCAGGACTAGCCATGGATGGCCTGTTTGATGACA
  GCGAAGATGAAAGTGATAAACTGAGCTACCCTTGGGATGGCACACAATCCTATTCATTGTTCAATGTGTC
  TCCTTCTTGTTCTTCTTTTAACTCTCCATGTAGAGATTCTGTGTCACCACCCAAATCCTTATTTTCTCAA
  AGACCCCAAAGGATGCGCTCTCGTTCAAGGTCCTTTTCTCGACACAGGTCGTGTTCCCGATCACCATATT
  CCAGGTCAAGATCAAGGTCTCCAGGCAGTAGATCCTCTTCAAGATCCTGCTATTACTATGAGTCAAGCCA
  CTACAGACACCGCACGCACCGAAATTCTCCCTTGTATGTGAGATCACGTTCAAGATCGCCCTACAGCCGT
  CGGCCCAGGTATGACAGCTACGAGGAATATCAGCACGAGAGGCTGAAGAGGGAAGAATATCGCAGAGAGT
  ATGAGAAGCGAGAGTCTGAGAGGGCCAAGCAAAGGGAGAGGCAGAGGCAGAAGGCAATTGAAGAGCGCCG
  TGTGATTTATGTCGGTAAAATCAGACCTGACACAACACGGACAGAACTGAGGGACCGTTTTGAAGTTTTT
  GGTGAAATTGAGGAGTGCACAGTAAATCTGCGGGATGATGGAGACAGCTATGGTTTCATTACCTACCGTT
  ATACCTGTGATGCTTTTGCTGCTCTTGAAAATGGATACACTTTGCGCAGGTCAAACGAAACTGACTTTGA
  GCTGTACTTTTGTGGACGCAAGCAATTTTTCAAGTCTAACTATGCAGACCTAGATTCAAACTCAGATGAC
  TTTGACCCTGCTTCCACCAAGAGCAAGTATGACTCTCTGGATTTTGATAGTTTACTGAAAGAAGCTCAGA
  GAAGCTTGCGCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212244 representing NM_013261
  Red=Cloning site Green=Tags(s)
  
  MAWDMCNQDSESVWSDIECAALVGEDQPLCPDLPELDLSELDVNDLDTDSFLGGLKWCSDQSEIISNQYN
  NEPSNIFEKIDEENEANLLAVLTETLDSLPVDEDGLPSFDALTDGDVTTDNEASPSSMPDGTPPPQEAEE
  PSLLKKLLLAPANTQLSYNECSGLSTQNHANHNHRIRTNPAIVKTENSWSNKAKSICQQQKPQRRPCSEL
  LKYLTTNDDPPHTKPTENRNSSRDKCTSKKKSHTQSQSQHLQAKPTTLSLPLTPESPNDPKGSPFENKTI
  ERTLSVELSGTAGLTPPTTPPHKANQDNPFRASPKLKSSCKTVVPPPSKKPRYSESSGTQGNNSTKKGPE
  QSELYAQLSKSSVLTGGHEERKTKRPSLRLFGDHDYCQSINSKTEILINISQELQDSRQLENKDVSSDWQ
  GQICSSTDSDQCYLRETLEASKQVSPCSTRKQLQDQEIRAELNKHFGHPSQAVFDDEADKTSELRDSDFS
  NEQFSKLPMFINSGLAMDGLFDDSEDESDKLSYPWDGTQSYSLFNVSPSCSSFNSPCRDSVSPPKSLFSQ
  RPQRMRSRSRSFSRHRSCSRSPYSRSRSRSPGSRSSSRSCYYYESSHYRHRTHRNSPLYVRSRSRSPYSR
  RPRYDSYEEYQHERLKREEYRREYEKRESERAKQRERQRQKAIEERRVIYVGKIRPDTTRTELRDRFEVF
  GEIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDSNSDD
  FDPASTKSKYDSLDFDSLLKEAQRSLRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013261
• ORF Size: 2394 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013261.5
• RefSeq Size: 6317 bp
• RefSeq ORF: 2397 bp
• Locus ID: 10891
• UniProt ID: Q9UBK2
• Cytogenetics: 4p15.2
• Domains: RRM
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Adipocytokine signaling pathway, Huntington's disease, Insulin signaling pathway
• MW: 90.8 kDa
• Gene Summary: The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008]