PGC1 alpha (PPARGC1A) (NM_013261) Human Tagged ORF Clone

CAT#: RC212244

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PPARGC1A (Myc-DDK-tagged)-Human peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (PPARGC1A)



  "NM_013261" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 691.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PPARGC1A
Synonyms LEM6; PGC-1(alpha); PGC-1alpha; PGC-1v; PGC1; PGC1A; PPARGC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC212244 representing NM_013261
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTGGGACATGTGCAACCAGGACTCTGAGTCTGTATGGAGTGACATCGAGTGTGCTGCTCTGGTTG
GTGAAGACCAGCCTCTTTGCCCAGATCTTCCTGAACTTGATCTTTCTGAACTAGATGTGAACGACTTGGA
TACAGACAGCTTTCTGGGTGGACTCAAGTGGTGCAGTGACCAATCAGAAATAATATCCAATCAGTACAAC
AATGAGCCTTCAAACATATTTGAGAAGATAGATGAAGAGAATGAGGCAAACTTGCTAGCAGTCCTCACAG
AGACACTAGACAGTCTCCCTGTGGATGAAGACGGATTGCCCTCATTTGATGCGCTGACAGATGGAGACGT
GACCACTGACAATGAGGCTAGTCCTTCCTCCATGCCTGACGGCACCCCTCCACCCCAGGAGGCAGAAGAG
CCGTCTCTACTTAAGAAGCTCTTACTGGCACCAGCCAACACTCAGCTAAGTTATAATGAATGCAGTGGTC
TCAGTACCCAGAACCATGCAAATCACAATCACAGGATCAGAACAAACCCTGCAATTGTTAAGACTGAGAA
TTCATGGAGCAATAAAGCGAAGAGTATTTGTCAACAGCAAAAGCCACAAAGACGTCCCTGCTCGGAGCTT
CTCAAATATCTGACCACAAACGATGACCCTCCTCACACCAAACCCACAGAGAACAGAAACAGCAGCAGAG
ACAAATGCACCTCCAAAAAGAAGTCCCACACACAGTCGCAGTCACAACACTTACAAGCCAAACCAACAAC
TTTATCTCTTCCTCTGACCCCAGAGTCACCAAATGACCCCAAGGGTTCCCCATTTGAGAACAAGACTATT
GAACGCACCTTAAGTGTGGAACTCTCTGGAACTGCAGGCCTAACTCCACCCACCACTCCTCCTCATAAAG
CCAACCAAGATAACCCTTTTAGGGCTTCTCCAAAGCTGAAGTCCTCTTGCAAGACTGTGGTGCCACCACC
ATCAAAGAAGCCCAGGTACAGTGAGTCTTCTGGTACACAAGGCAATAACTCCACCAAGAAAGGGCCGGAG
CAATCCGAGTTGTATGCACAACTCAGCAAGTCCTCAGTCCTCACTGGTGGACACGAGGAAAGGAAGACCA
AGCGGCCCAGTCTGCGGCTGTTTGGTGACCATGACTATTGCCAGTCAATTAATTCCAAAACGGAAATACT
CATTAATATATCACAGGAGCTCCAAGACTCTAGACAACTAGAAAATAAAGATGTCTCCTCTGATTGGCAG
GGGCAGATTTGTTCTTCCACAGATTCAGACCAGTGCTACCTGAGAGAGACTTTGGAGGCAAGCAAGCAGG
TCTCTCCTTGCAGCACAAGAAAACAGCTCCAAGACCAGGAAATCCGAGCCGAGCTGAACAAGCACTTCGG
TCATCCCAGTCAAGCTGTTTTTGACGACGAAGCAGACAAGACCAGTGAACTGAGGGACAGTGATTTCAGT
AATGAACAATTCTCCAAACTACCTATGTTTATAAATTCAGGACTAGCCATGGATGGCCTGTTTGATGACA
GCGAAGATGAAAGTGATAAACTGAGCTACCCTTGGGATGGCACACAATCCTATTCATTGTTCAATGTGTC
TCCTTCTTGTTCTTCTTTTAACTCTCCATGTAGAGATTCTGTGTCACCACCCAAATCCTTATTTTCTCAA
AGACCCCAAAGGATGCGCTCTCGTTCAAGGTCCTTTTCTCGACACAGGTCGTGTTCCCGATCACCATATT
CCAGGTCAAGATCAAGGTCTCCAGGCAGTAGATCCTCTTCAAGATCCTGCTATTACTATGAGTCAAGCCA
CTACAGACACCGCACGCACCGAAATTCTCCCTTGTATGTGAGATCACGTTCAAGATCGCCCTACAGCCGT
CGGCCCAGGTATGACAGCTACGAGGAATATCAGCACGAGAGGCTGAAGAGGGAAGAATATCGCAGAGAGT
ATGAGAAGCGAGAGTCTGAGAGGGCCAAGCAAAGGGAGAGGCAGAGGCAGAAGGCAATTGAAGAGCGCCG
TGTGATTTATGTCGGTAAAATCAGACCTGACACAACACGGACAGAACTGAGGGACCGTTTTGAAGTTTTT
GGTGAAATTGAGGAGTGCACAGTAAATCTGCGGGATGATGGAGACAGCTATGGTTTCATTACCTACCGTT
ATACCTGTGATGCTTTTGCTGCTCTTGAAAATGGATACACTTTGCGCAGGTCAAACGAAACTGACTTTGA
GCTGTACTTTTGTGGACGCAAGCAATTTTTCAAGTCTAACTATGCAGACCTAGATTCAAACTCAGATGAC
TTTGACCCTGCTTCCACCAAGAGCAAGTATGACTCTCTGGATTTTGATAGTTTACTGAAAGAAGCTCAGA
GAAGCTTGCGCAGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC212244 representing NM_013261
Red=Cloning site Green=Tags(s)

MAWDMCNQDSESVWSDIECAALVGEDQPLCPDLPELDLSELDVNDLDTDSFLGGLKWCSDQSEIISNQYN
NEPSNIFEKIDEENEANLLAVLTETLDSLPVDEDGLPSFDALTDGDVTTDNEASPSSMPDGTPPPQEAEE
PSLLKKLLLAPANTQLSYNECSGLSTQNHANHNHRIRTNPAIVKTENSWSNKAKSICQQQKPQRRPCSEL
LKYLTTNDDPPHTKPTENRNSSRDKCTSKKKSHTQSQSQHLQAKPTTLSLPLTPESPNDPKGSPFENKTI
ERTLSVELSGTAGLTPPTTPPHKANQDNPFRASPKLKSSCKTVVPPPSKKPRYSESSGTQGNNSTKKGPE
QSELYAQLSKSSVLTGGHEERKTKRPSLRLFGDHDYCQSINSKTEILINISQELQDSRQLENKDVSSDWQ
GQICSSTDSDQCYLRETLEASKQVSPCSTRKQLQDQEIRAELNKHFGHPSQAVFDDEADKTSELRDSDFS
NEQFSKLPMFINSGLAMDGLFDDSEDESDKLSYPWDGTQSYSLFNVSPSCSSFNSPCRDSVSPPKSLFSQ
RPQRMRSRSRSFSRHRSCSRSPYSRSRSRSPGSRSSSRSCYYYESSHYRHRTHRNSPLYVRSRSRSPYSR
RPRYDSYEEYQHERLKREEYRREYEKRESERAKQRERQRQKAIEERRVIYVGKIRPDTTRTELRDRFEVF
GEIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDSNSDD
FDPASTKSKYDSLDFDSLLKEAQRSLRR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_013261
ORF Size 2394 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_013261.5
RefSeq Size 6317 bp
RefSeq ORF 2397 bp
Locus ID 10891
UniProt ID Q9UBK2
Cytogenetics 4p15.2
Domains RRM
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Adipocytokine signaling pathway, Huntington's disease, Insulin signaling pathway
MW 90.8 kDa
Gene Summary The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008]

Other Versions

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Customer Reviews 
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