TBL1Y (NM_134259) Human Tagged ORF Clone

CAT#: RC212240

TBL1Y (Myc-DDK-tagged)-Human transducin (beta)-like 1, Y-linked (TBL1Y), transcript variant 3

Product Images

Circular map for RC212240

• 

  Western blot validation of overexpression lysate (Cat# LY409606) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220139 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified TBL1Y protein (Cat# TP312240). The protein was produced from HEK293T cells transfected with TBL1Y cDNA clone (Cat# RC212240) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TBL1Y
• Synonyms: DFNY2; TBL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212240 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCATAACCAGTGACGAGGTGAACTTTCTGGTTTATCGGTATCTCCAGGAGTCAGGTTTTTCTCACT
  CGGCTTTCACGTTTGGGATCGAGAGCCACATCAGCCAGTCCAACATCAATGGGACACTAGTGCCACCGTC
  TGCCCTCATCTCCATTCTCCAGAAGGGACTGCAGTATGTAGAGGCTGAGATAAGCATCAACAAGGATGGC
  ACAGTGTTCGACAGCCGCCCTATAGAGTCCCTGTCCCTGATAGTTGCTGTGATTCCTGATGTGGTGCAGA
  TGCGGCAGCAGGCATTTGGAGAGAAGCTCACTCAGCAGCAAGCCAGTGCAGCAGCCACAGAGGCATCAGC
  AATGGCAAAGGCGGCAACCATGACCCCAGCTGCTATTTCCCAGCAAAATCCTCCAAAGAACCGAGAGGCC
  ACAGTGAACGGGGAAGAGAATGGAGCACATGAAATCAATAATCACTCAAAGCCAATGGAAATAGATGGGG
  ATGTTGAGATTCCACCCAACAAAGCCACAGTCCTTCGGGGCCACGAGTCTGAGGTGTTCATTTGTGCCTG
  GAACCCTGTCAGTGATTTGCTAGCCTCTGGATCTGGAGACTCAACTGCAAGGATATGGAACCTGAATGAG
  AATAGCAACGGGGGCTCCACCCAGCTCGTGTTGAGACATTGTATACGAGAAGGGGGGCACGACGTCCCAA
  GTAATAAAGATGTCACCTCACTGGACTGGAACAGTGATGGAACACTATTGGCTATGGGTTCATATGATGG
  TTTCGCAAGAATATGGACAGAAAATGGTAACCTGGCCAGCACCTTAGGCCAACATAAAGGCCCCATCTTC
  GCTCTGAAATGGAACAAAAAGGGGAATTATGTTTTGAGTGCTGGTGTAGACAAAACAACAATAATTTGGG
  ATGCTCACACAGGAGAAGCCAAACAGCAGTTTCCTTTTCATTCAGCCCCCGCCCTTGATGTGGACTGGCA
  GAACAACATGACCTTTGCCTCCTGTAGCACAGACATGTGTATCCATGTGTGCAGGCTCGGCTGTGACCAC
  CCAGTCAAAACCTTCCAGGGACACACAAACGAGGTCAATGCCATCAAATGGGATCCTTCTGGAATGTTGC
  TGGCGTCCTGCTCGGATGACATGACATTGAAGATCTGGAGTATGAAGCAGGATGCATGCGTCCACGATCT
  TCAGGCTCACAGCAAAGAGATCTACACCATCAAGTGGAGCCCCACCGGGCCTGCCACCAGCAACCCAAAC
  TCCAGCATCATGTTGGCAAGTGCTTCATTTGATTCTACAGTGCGACTGTGGGATGTGGAGCAAGGTGTCT
  GCACCCACACACTCATGAAGCATCAAGAGCCTGTCTACAGTGTAGCTTTCAGCCCCGATGGAAAGTACTT
  GGCTAGTGGATCCTTTGACAAGTATGTTCATATCTGGAATACTCAGAGTGGAAGTCTCGTCCACAGCTAC
  CAAGGCACTGGCGGTATCTTCGAGGTGTGCTGGAACGCCCGAGGAGATAAAGTGGGCGCCAGCGCGTCTG
  ATGGCTCTGTGTGTGTTCTGGATCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212240 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSITSDEVNFLVYRYLQESGFSHSAFTFGIESHISQSNINGTLVPPSALISILQKGLQYVEAEISINKDG
  TVFDSRPIESLSLIVAVIPDVVQMRQQAFGEKLTQQQASAAATEASAMAKAATMTPAAISQQNPPKNREA
  TVNGEENGAHEINNHSKPMEIDGDVEIPPNKATVLRGHESEVFICAWNPVSDLLASGSGDSTARIWNLNE
  NSNGGSTQLVLRHCIREGGHDVPSNKDVTSLDWNSDGTLLAMGSYDGFARIWTENGNLASTLGQHKGPIF
  ALKWNKKGNYVLSAGVDKTTIIWDAHTGEAKQQFPFHSAPALDVDWQNNMTFASCSTDMCIHVCRLGCDH
  PVKTFQGHTNEVNAIKWDPSGMLLASCSDDMTLKIWSMKQDACVHDLQAHSKEIYTIKWSPTGPATSNPN
  SSIMLASASFDSTVRLWDVEQGVCTHTLMKHQEPVYSVAFSPDGKYLASGSFDKYVHIWNTQSGSLVHSY
  QGTGGIFEVCWNARGDKVGASASDGSVCVLDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134259
• ORF Size: 1566 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134259.1, NP_599021.1
• RefSeq Size: 2312 bp
• RefSeq ORF: 1569 bp
• Locus ID: 90665
• UniProt ID: Q9BQ87
• Cytogenetics: Yp11.2
• Domains: WD40, LisH
• Protein Families: Transcription Factors
• Protein Pathways: Wnt signaling pathway
• MW: 56.7 kDa
• Gene Summary: The protein encoded by this gene has sequence similarity with members of the WD40 repeat-containing protein family. The WD40 group is a large family of proteins, which appear to have a regulatory function. It is believed that the WD40 repeats mediate protein-protein interactions and members of the family are involved in signal transduction, RNA processing, gene regulation, vesicular trafficking, cytoskeletal assembly and may play a role in the control of cytotypic differentiation. This gene is highly similar to TBL1X gene in nucleotide sequence and protein sequence, but the TBL1X gene is located on chromosome X and this gene is on chromosome Y. This gene has three alternatively spliced transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]