DNASE2B (NM_021233) Human Tagged ORF Clone
Lenti ORF clone of Human deoxyribonuclease II beta (DNASE2B), transcript variant 1, Myc-DDK-tagged
|Product Name||DNASE2B (NM_021233) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||1071 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|Gene Summary||The protein encoded by this gene shares considerable sequence similarity to, and is structurally related to DNase II. The latter is a well characterized endonuclease that catalyzes DNA hydrolysis in the absence of divalent cations at acidic pH. Unlike DNase II which is ubiquitously expressed, expression of this gene product is restricted to the salivary gland and lungs. The gene has been localized to chromosome 1p22.3 adjacent (and in opposite orientation) to the uricase pseudogene. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|SC304928||DNASE2B (untagged)-Human deoxyribonuclease II beta (DNASE2B), transcript variant 1||
|RC212192||DNASE2B (Myc-DDK-tagged)-Human deoxyribonuclease II beta (DNASE2B), transcript variant 1||
|RG212192||DNASE2B (GFP-tagged) - Human deoxyribonuclease II beta (DNASE2B), transcript variant 1||
|RC212192L1||Lenti ORF clone of Human deoxyribonuclease II beta (DNASE2B), transcript variant 1, Myc-DDK-tagged||
|RC212192L2||Lenti ORF clone of Human deoxyribonuclease II beta (DNASE2B), transcript variant 1, mGFP tagged||
|RC212192L4||Lenti ORF clone of Human deoxyribonuclease II beta (DNASE2B), transcript variant 1, mGFP tagged||