Annexin A11 (ANXA11) (NM_001157) Human Tagged ORF Clone

CAT#: RC212191

ANXA11 (Myc-DDK-tagged)-Human annexin A11 (ANXA11), transcript variant a

Product Images

Circular map for RC212191

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ANXA11 (Cat# RC212191, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA11 (Cat# TA500950). Positive lysates LY420097 (100ug) and LC420097 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY420097) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212191 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ANXA11 protein (Cat# TP312191). The protein was produced from HEK293T cells transfected with ANXA11 cDNA clone (Cat# RC212191) using MegaTran 2.0 (Cat# TT210002).

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  Anti-ANXA11 mouse monoclonal antibody (TA500950) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANXA11 (RC212191).

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  HEK293T cells transfected with either pCMV6-ENTRY ANXA11 (RC212191) (Red) or empty vector control plasmid (Blue) were immunostained with anti-ANXA11 mouse monoclonal (TA500950), and then analyzed by flow cytometry.

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Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Annexin A11
• Synonyms: ALS23; ANX11; CAP-50; CAP50
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212191 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTACCCTGGCTATCCCCCGCCCCCAGGTGGCTACCCACCAGCTGCACCAGGTGGTGGTCCCTGGG
  GAGGTGCTGCCTACCCTCCTCCGCCCAGCATGCCCCCCATCGGGCTGGATAACGTGGCCACCTATGCGGG
  GCAGTTCAACCAGGACTATCTCTCGGGAATGGCGGCCAACATGTCTGGGACATTTGGAGGAGCCAACATG
  CCCAACCTGTACCCTGGGGCCCCTGGGGCTGGCTACCCACCAGTGCCCCCTGGCGGCTTTGGGCAGCCCC
  CCTCTGCCCAGCAGCCTGTTCCTCCCTATGGGATGTATCCACCCCCAGGAGGAAACCCACCCTCCAGGAT
  GCCCTCATATCCGCCATACCCAGGGGCCCCTGTGCCGGGCCAGCCCATGCCACCCCCCGGACAGCAGCCC
  CCAGGGGCCTACCCTGGGCAGCCACCAGTGACCTACCCTGGTCAGCCTCCAGTGCCACTCCCTGGGCAGC
  AGCAGCCAGTGCCGAGCTACCCAGGATACCCGGGGTCTGGGACTGTCACCCCCGCTGTGCCCCCAACCCA
  GTTTGGAAGCCGAGGCACCATCACTGATGCTCCCGGCTTTGACCCCCTGCGAGATGCCGAGGTCCTGCGG
  AAGGCCATGAAAGGCTTCGGGACGGATGAGCAGGCCATCATTGACTGCCTGGGGAGTCGCTCCAACAAGC
  AGCGGCAGCAGATCCTACTTTCCTTCAAGACGGCTTACGGCAAGGATTTGATCAAAGATCTGAAATCTGA
  ACTGTCAGGAAACTTTGAGAAGACAATCTTGGCTCTGATGAAGACCCCAGTCCTCTTTGACATTTATGAG
  ATAAAGGAAGCCATCAAGGGGGTTGGCACTGATGAAGCCTGCCTGATTGAGATCCTCGCTTCCCGCAGCA
  ATGAGCACATCCGAGAATTAAACAGAGCCTACAAAGCAGAATTCAAAAAGACCCTGGAAGAGGCCATTCG
  AAGCGACACATCAGGGCACTTCCAGCGGCTCCTCATCTCTCTCTCTCAGGGAAACCGTGATGAAAGCACA
  AACGTGGACATGTCACTCGCCCAGAGAGATGCCCAGGAGCTGTATGCGGCCGGGGAGAACCGCCTGGGAA
  CAGACGAGTCCAAGTTCAATGCGGTTCTGTGCTCCCGGAGCCGGGCCCACCTGGTAGCAGTTTTCAATGA
  GTACCAGAGAATGACAGGCCGGGACATTGAGAAGAGCATCTGCCGGGAGATGTCCGGGGACCTGGAGGAG
  GGCATGCTGGCCGTGGTGAAATGTCTCAAGAATACCCCAGCCTTCTTTGCGGAGAGGCTCAACAAGGCCA
  TGAGGGGGGCAGGAACAAAGGACCGGACCCTGATTCGCATCATGGTGTCTCGCAGCGAGACCGACCTCCT
  GGACATCAGATCAGAGTATAAGCGGATGTACGGCAAGTCGCTGTACCACGACATCTCGGGAGATACTTCA
  GGGGATTACCGGAAGATTCTGCTGAAGATCTGTGGTGGCAATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212191 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSYPGYPPPPGGYPPAAPGGGPWGGAAYPPPPSMPPIGLDNVATYAGQFNQDYLSGMAANMSGTFGGANM
  PNLYPGAPGAGYPPVPPGGFGQPPSAQQPVPPYGMYPPPGGNPPSRMPSYPPYPGAPVPGQPMPPPGQQP
  PGAYPGQPPVTYPGQPPVPLPGQQQPVPSYPGYPGSGTVTPAVPPTQFGSRGTITDAPGFDPLRDAEVLR
  KAMKGFGTDEQAIIDCLGSRSNKQRQQILLSFKTAYGKDLIKDLKSELSGNFEKTILALMKTPVLFDIYE
  IKEAIKGVGTDEACLIEILASRSNEHIRELNRAYKAEFKKTLEEAIRSDTSGHFQRLLISLSQGNRDEST
  NVDMSLAQRDAQELYAAGENRLGTDESKFNAVLCSRSRAHLVAVFNEYQRMTGRDIEKSICREMSGDLEE
  GMLAVVKCLKNTPAFFAERLNKAMRGAGTKDRTLIRIMVSRSETDLLDIRSEYKRMYGKSLYHDISGDTS
  GDYRKILLKICGGND
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001157
• ORF Size: 1515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001157.3
• RefSeq Size: 2486 bp
• RefSeq ORF: 1518 bp
• Locus ID: 311
• UniProt ID: P50995
• Cytogenetics: 10q22.3
• Domains: annexin
• MW: 54.4 kDa
• Gene Summary: This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Several transcript variants encoding two different isoforms have been identified. [provided by RefSeq, Dec 2015]