VSIG8 (NM_001013661) Human Tagged ORF Clone

CAT#: RC212118

VSIG8 (Myc-DDK-tagged)-Human V-set and immunoglobulin domain containing 8 (VSIG8)

Product Images

Circular map for RC212118

• 

  Western blot validation of overexpression lysate (Cat# LY423010) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212118 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified VSIG8 protein (Cat# TP312118). The protein was produced from HEK293T cells transfected with VSIG8 cDNA clone (Cat# RC212118) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VSIG8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212118 representing NM_001013661
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGTTGGAGGAGCATTCCACCTTCTACTCGTGTGCCTGAGCCCAGCACTGCTGTCTGCTGTGCGGA
  TCAACGGGGATGGACAGGAGGTCCTGTACCTGGCAGAAGGTGATAATGTGAGGCTGGGCTGCCCCTACGT
  CCTGGACCCTGAGGACTATGGTCCCAATGGGCTGGACATCGAGTGGATGCAGGTCAACTCAGACCCCGCC
  CACCACCGAGAGAACGTGTTCCTTAGTTACCAGGACAAGAGGATCAACCATGGCAGCCTTCCCCATCTGC
  AGCAGAGGGTCCGCTTTGCAGCCTCAGACCCAAGCCAGTACGATGCCTCCATCAACCTCATGAACCTGCA
  GGTATCTGATACAGCCACTTATGAGTGCCGGGTGAAGAAGACCACCATGGCCACCCGGAAGGTCATTGTC
  ACTGTCCAAGCACGACCTGCAGTGCCCATGTGCTGGACAGAGGGCCACATGACATATGGCAACGATGTGG
  TGCTGAAGTGCTATGCCAGTGGGGGCTCCCAGCCCCTCTCCTACAAGTGGGCCAAGATCAGTGGGCACCA
  TTACCCCTATCGAGCTGGGTCTTACACCTCCCAGCACAGCTACCACTCAGAGCTGTCCTACCAGGAGTCC
  TTCCACAGCTCCATAAACCAAGGCCTGAACAATGGGGACCTGGTGTTGAAGGATATCTCCAGAGCAGATG
  ATGGGCTGTATCAGTGCACAGTGGCCAACAACGTGGGCTACAGTGTTTGTGTGGTGGAGGTGAAGGTCTC
  AGACTCCCGGCGTATAGGCGTGATCATCGGCATCGTCCTGGGCTCTCTGCTCGCGCTGGGCTGCCTGGCC
  GTAGGCATCTGGGGGCTCGTCTGCTGCTGCTGCGGGGGCTCCGGGGCTGGCGGCGCCCGCGGTGCCTTCG
  GCTACGGCAACGGCGGCGGGGTCGGCGGAGGGGCCTGCGGCGACTTGGCTAGTGAGATCAGAGAGGACGC
  CGTGGCGCCCGGGTGCAAGGCCAGCGGGCGCGGCAGCCGCGTCACCCACCTCCTGGGGTACCCGACGCAG
  AACGTCAGCCGCTCCCTGCGCCGCAAGTACGCGCCTCCCCCCTGCGGCGGCCCCGAGGACGTGGCCCTGG
  CGCCCTGCACCGCCGCCGCCGCCTGCGAAGCGGGCCCCTCCCCGGTCTACGTCAAGGTCAAGAGCGCGGA
  GCCGGCTGACTGCGCCGAGGGGCCGGTGCAGTGCAAGAACGGCCTCTTGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212118 representing NM_001013661
  Red=Cloning site Green=Tags(s)
  
  MRVGGAFHLLLVCLSPALLSAVRINGDGQEVLYLAEGDNVRLGCPYVLDPEDYGPNGLDIEWMQVNSDPA
  HHRENVFLSYQDKRINHGSLPHLQQRVRFAASDPSQYDASINLMNLQVSDTATYECRVKKTTMATRKVIV
  TVQARPAVPMCWTEGHMTYGNDVVLKCYASGGSQPLSYKWAKISGHHYPYRAGSYTSQHSYHSELSYQES
  FHSSINQGLNNGDLVLKDISRADDGLYQCTVANNVGYSVCVVEVKVSDSRRIGVIIGIVLGSLLALGCLA
  VGIWGLVCCCCGGSGAGGARGAFGYGNGGGVGGGACGDLASEIREDAVAPGCKASGRGSRVTHLLGYPTQ
  NVSRSLRRKYAPPPCGGPEDVALAPCTAAAACEAGPSPVYVKVKSAEPADCAEGPVQCKNGLLV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013661
• ORF Size: 1242 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013661.1, NP_001013683.1
• RefSeq Size: 1818 bp
• RefSeq ORF: 1245 bp
• Locus ID: 391123
• UniProt ID: Q5VU13
• Cytogenetics: 1q23.2
• Protein Families: Transmembrane
• MW: 43.7 kDa