MTMR1 (NM_003828) Human Tagged ORF Clone

CAT#: RC212024

MTMR1 (Myc-DDK-tagged)-Human myotubularin related protein 1 (MTMR1)

Product Images

Circular map for RC212024

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  Western blot validation of overexpression lysate (Cat# LY418400) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212024 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MTMR1 protein (Cat# TP312024). The protein was produced from HEK293T cells transfected with MTMR1 cDNA clone (Cat# RC212024) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MTMR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212024 representing NM_003828
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGGCCGGCGGCGGCGGCGGCGGCGGGCTGCGAGGGCGGCGGGGGCCCGAACCCGGGGCCGGCGG
  GCGGCAGGAGGCCTCCTCGGGCCGCGGGGGGCGCCACCGCCGGCTCCCGGCAGCCCAGCGTGGAGACCCT
  GGACAGTCCCACAGGATCACATGTTGAATGGTGTAAACAGCTTATAGCTGCTACAATTTCTAGTCAGATT
  TCAGGTTCAGTGACATCAGAAAATGTGTCCAGAGATTACAAGGCTCTAAGGGATGGAAATAAGCTGGCAC
  AGATGGAAGAGGCTCCACTTTTCCCAGGAGAATCAATTAAAGCCATTGTGAAAGATGTCATGTATATCTG
  CCCATTTATGGGAGCAGTGAGTGGAACCCTGACAGTGACGGACTTTAAGCTGTACTTCAAAAATGTCGAG
  AGGGACCCGCATTTTATCCTTGATGTTCCCCTTGGAGTGATCAGCAGAGTGGAGAAGATTGGAGCACAGA
  GCCATGGAGACAATTCCTGTGGTATAGAGATAGTGTGCAAGGATATGAGGAACTTGCGGCTTGCTTATAA
  ACAGGAAGAACAGAGTAAACTAGGGATATTTGAAAACCTCAACAAACATGCATTTCCTCTTTCTAACGGA
  CAGGCACTATTTGCATTCAGCTATAAAGAAAAATTTCCAATTAATGGCTGGAAAGTTTATGATCCAGTAT
  CTGAATATAAGAGACAGGGCTTGCCAAATGAGAGTTGGAAAATATCCAAAATAAACAGTAATTATGAGTT
  CTGTGACACCTACCCTGCCATCATTGTTGTGCCAACTAGTGTAAAAGATGATGACCTTTCAAAAGTGGCA
  GCTTTTCGAGCAAAAGGCAGAGTCCCTGTGTTGTCATGGATTCATCCGGAAAGTCAAGCAACGATTACCC
  GTTGCAGCCAGCCACTTGTGGGTCCCAATGATAAGCGCTGCAAAGAGGATGAAAAATACTTGCAAACAAT
  AATGGATGCTAACGCACAGTCACACAAGCTTATCATCTTTGATGCTCGACAAAACAGTGTCGCTGATACC
  AACAAGACAAAGGGTGGAGGATATGAAAGTGAAAGTGCTTACCCAAATGCAGAACTTGTGTTCTTGGAGA
  TCCACAACATTCATGTCATGCGAGAGTCACTACGCAAATTAAAAGAGATTGTGTACCCTTCGATCGATGA
  GGCGCGGTGGCTCTCCAATGTGGATGGGACGCATTGGCTGGAATATATAAGGATGCTGCTTGCTGGGGCA
  GTAAGAATTGCTGATAAAATAGAATCTGGGAAAACATCTGTGGTGGTGCATTGCAGCGACGGTTGGGACC
  GAACAGCCCAGCTCACATCTCTGGCTATGCTAATGTTGGACAGTTACTACAGGACCATTAAAGGATTTGA
  AACTCTCGTAGAAAAGGAGTGGATAAGCTTTGGACACAGGTTTGCACTGCGAGTGGGCCATGGTAATGAC
  AACCATGCGGATGCTGACCGATCTCCCATATTTCTGCAGTTTGTTGATTGTGTTTGGCAAATGACAAGGC
  AGTTTCCTTCAGCATTCGAGTTTAATGAGCTATTCTTGATTACAATTTTGGATCACCTTTATAGCTGTCT
  TTTTGGGACCTTTTTGTGCAACTGTGAACAGCAGCGATTCAAAGAGGATGTATATACAAAGACGATATCT
  TTATGGTCGTATATCAATAGCCAGCTAGACGAGTTTTCTAATCCCTTCTTTGTGAATTATGAAAACCACG
  TGTTATATCCTGTTGCTAGTCTGAGTCATTTGGAATTGTGGGTAAATTATTATGTACGATGGAATCCACG
  GATGAGACCTCAGATGCCCATTCACCAGAATCTCAAGGAGCTGCTGGCCGTCAGGGCGGAGCTGCAGAAG
  CGTGTGGAGGGCCTACAGCGGGAGGTGGCCACGCGCGCCGTCTCATCCTCATCTGAGCGGGGCTCCTCGC
  CCTCCCACTCCGCCACCTCCGTCCACACCTCGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212024 representing NM_003828
  Red=Cloning site Green=Tags(s)
  
  MDRPAAAAAAGCEGGGGPNPGPAGGRRPPRAAGGATAGSRQPSVETLDSPTGSHVEWCKQLIAATISSQI
  SGSVTSENVSRDYKALRDGNKLAQMEEAPLFPGESIKAIVKDVMYICPFMGAVSGTLTVTDFKLYFKNVE
  RDPHFILDVPLGVISRVEKIGAQSHGDNSCGIEIVCKDMRNLRLAYKQEEQSKLGIFENLNKHAFPLSNG
  QALFAFSYKEKFPINGWKVYDPVSEYKRQGLPNESWKISKINSNYEFCDTYPAIIVVPTSVKDDDLSKVA
  AFRAKGRVPVLSWIHPESQATITRCSQPLVGPNDKRCKEDEKYLQTIMDANAQSHKLIIFDARQNSVADT
  NKTKGGGYESESAYPNAELVFLEIHNIHVMRESLRKLKEIVYPSIDEARWLSNVDGTHWLEYIRMLLAGA
  VRIADKIESGKTSVVVHCSDGWDRTAQLTSLAMLMLDSYYRTIKGFETLVEKEWISFGHRFALRVGHGND
  NHADADRSPIFLQFVDCVWQMTRQFPSAFEFNELFLITILDHLYSCLFGTFLCNCEQQRFKEDVYTKTIS
  LWSYINSQLDEFSNPFFVNYENHVLYPVASLSHLELWVNYYVRWNPRMRPQMPIHQNLKELLAVRAELQK
  RVEGLQREVATRAVSSSSERGSSPSHSATSVHTSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003828
• ORF Size: 1995 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003828.4
• RefSeq Size: 2754 bp
• RefSeq ORF: 1998 bp
• Locus ID: 8776
• UniProt ID: Q13613
• Cytogenetics: Xq28
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Fructose and mannose metabolism, Metabolic pathways, Riboflavin metabolism, Thiamine metabolism
• MW: 74.5 kDa
• Gene Summary: This gene encodes a member of the myotubularin related family of proteins. Members of this family contain the consensus sequence for the active site of protein tyrosine phosphatases. Alternatively spliced variants have been described but their biological validity has not been determined. [provided by RefSeq, Jul 2008]