SMOC2 (NM_022138) Human Tagged ORF Clone

CAT#: RC211979

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SMOC2 (Myc-DDK-tagged)-Human SPARC related modular calcium binding 2 (SMOC2), transcript variant 1



  "NM_022138" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 488.00


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In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SMOC2
Synonyms bA37D8.1; bA270C4A.1; dJ421D16.1; DTDP1; MST117; MSTP117; MSTP140; SMAP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211979 representing NM_022138
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCTCCCCCAGCTCTGCTGGCTGCCGCTGCTCGCTGGGCTGCTCCCGCCGGTGCCCGCTCAGAAGT
TCTCGGCGCTCACGTTTTTGAGAGTGGATCAAGATAAAGACAAGGATTGTAGCTTGGACTGTGCGGGTTC
GCCCCAGAAACCTCTCTGCGCATCTGACGGAAGGACCTTCCTTTCCCGTTGTGAATTTCAACGTGCCAAG
TGCAAAGATCCCCAGCTAGAGATTGCATATCGAGGAAACTGCAAAGACGTGTCCAGGTGTGTGGCCGAAA
GGAAGTATACCCAGGAGCAAGCCCGGAAGGAGTTTCAGCAAGTGTTCATTCCTGAGTGCAATGACGACGG
CACCTACAGTCAGGTCCAGTGTCACAGCTACACGGGATACTGCTGGTGCGTCACGCCCAACGGGAGGCCC
ATCAGCGGCACTGCCGTGGCCCACAAGACGCCCCGGTGCCCGGGTTCCGTAAATGAAAAGTTACCCCAAC
GCGAAGGCACAGGAAAAACAGTCTCCTTGCAAATCTTTTCCGTTCTGAATTCAGATGATGCCGCAGCTCC
AGCGTTGGAGACTCAGCCTCAAGGAGATGAAGAAGATATTGCATCACGTTACCCTACCCTTTGGACTGAA
CAGGTTAAAAGTCGGCAGAACAAAACCAATAAGAATTCAGTGTCATCCTGTGACCAAGAGCACCAGTCTG
CCCTGGAGGAAGCCAAGCAGCCCAAGAACGACAATGTGGTGATCCCTGAGTGTGCGCACGGCGGCCTCTA
CAAGCCAGTGCAGTGCCACCCCTCCACGGGGTACTGCTGGTGCGTCCTGGTGGACACGGGGCGCCCCATT
CCCGGCACATCCACAAGGTACGAGCAGCCGAAATGTGACAACACGGCCAGGGCCCACCCAGCCAAAGCCC
GGGACCTGTACAAGGGCCGCCAGCTACAAGGTTGTCCGGGTGCCAAAAAGCATGAGTTTCTGACCAGCGT
TCTGGACGCGCTGTCCACGGACATGGTCCACGCCGCCTCCGACCCCTCCTCCTCGTCAGGCAGGCTCTCA
GAACCCGACCCCAGCCATACCCTAGAGGAGCGGGTGGTGCACTGGTACTTCAAACTACTGGATAAAAACT
CCAGTGGAGACATCGGCAAAAAGGAAATCAAACCCTTCAAGAGGTTCCTTCGCAAAAAATCAAAGCCCAA
AAAATGTGTGAAGAAGTTTGTTGAATACTGTGACGTGAATAATGACAAATCCATCTCCGTACAAGAACTG
ATGGGCTGCCTGGGCGTGGCGAAAGAGGACGGCAAAGCGGACACCAAGAAACGCCACACCCCCAGAGGTC
ATGCTGAAAGTACGTCTAATAGACAGCCAAGGAAACAAGGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211979 representing NM_022138
Red=Cloning site Green=Tags(s)

MLLPQLCWLPLLAGLLPPVPAQKFSALTFLRVDQDKDKDCSLDCAGSPQKPLCASDGRTFLSRCEFQRAK
CKDPQLEIAYRGNCKDVSRCVAERKYTQEQARKEFQQVFIPECNDDGTYSQVQCHSYTGYCWCVTPNGRP
ISGTAVAHKTPRCPGSVNEKLPQREGTGKTVSLQIFSVLNSDDAAAPALETQPQGDEEDIASRYPTLWTE
QVKSRQNKTNKNSVSSCDQEHQSALEEAKQPKNDNVVIPECAHGGLYKPVQCHPSTGYCWCVLVDTGRPI
PGTSTRYEQPKCDNTARAHPAKARDLYKGRQLQGCPGAKKHEFLTSVLDALSTDMVHAASDPSSSSGRLS
EPDPSHTLEERVVHWYFKLLDKNSSGDIGKKEIKPFKRFLRKKSKPKKCVKKFVEYCDVNNDKSISVQEL
MGCLGVAKEDGKADTKKRHTPRGHAESTSNRQPRKQG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_022138
ORF Size 1371 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_022138.3
RefSeq Size 2947 bp
RefSeq ORF 1374 bp
Locus ID 64094
UniProt ID Q9H3U7
Cytogenetics 6q27
Domains thyroglobulin_1, EFh, kazal
Protein Families Secreted Protein
MW 48.6 kDa
Gene Summary This gene encodes a member of the SPARC family (secreted protein acidic and rich in cysteine/osteonectin/BM-40), which are highly expressed during embryogenesis and wound healing. The gene product is a matricellular protein which promotes matrix assembly and can stimulate endothelial cell proliferation and migration, as well as angiogenic activity. Associated with pulmonary function, this secretory gene product contains a Kazal domain, two thymoglobulin type-1 domains, and two EF-hand calcium-binding domains. The encoded protein may serve as a target for controlling angiogenesis in tumor growth and myocardial ischemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Other Versions

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