ROBO1 (NM_133631) Human Tagged ORF Clone

CAT#: RC211961

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ROBO1 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 2


 Cited in 1 publication.

  View "NM_133631" in other vectors (10)

USD 1,700.00


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    • 10 ug


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Specifications

Product Data
Product Name ROBO1 (NM_133631) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ROBO1
Synonyms DUTT1; SAX3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC211961 representing NM_133631
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATTGCGGAGCCCGCTCACTTTTACCTGTTTGGATTAATATGTCTCTGTTCAGGCTCCCGTCTTCGTC
AGGAAGATTTTCCACCTCGCATTGTTGAACACCCTTCAGACCTGATTGTCTCAAAAGGAGAACCTGCAAC
TTTGAACTGCAAAGCTGAAGGCCGCCCCACACCCACTATTGAATGGTACAAAGGGGGAGAGAGAGTGGAG
ACAGACAAAGATGACCCTCGCTCACACCGAATGTTGCTGCCGAGTGGATCTTTATTTTTCTTACGTATAG
TACATGGACGGAAAAGTAGACCTGATGAAGGAGTCTATGTCTGTGTAGCAAGGAATTACCTTGGAGAGGC
TGTGAGCCACAATGCATCGCTGGAAGTAGCCATACTTCGGGATGACTTCAGACAAAACCCTTCGGATGTC
ATGGTTGCAGTAGGAGAGCCTGCAGTAATGGAATGCCAACCTCCACGAGGCCATCCTGAGCCCACCATTT
CATGGAAGAAAGATGGCTCTCCACTGGATGATAAAGATGAAAGAATAACTATACGAGGAGGAAAGCTCAT
GATCACTTACACCCGTAAAAGTGACGCTGGCAAATATGTTTGTGTTGGTACCAATATGGTTGGGGAACGT
GAGAGTGAAGTAGCCGAGCTGACTGTCTTAGAGAGACCATCATTTGTGAAGAGACCCAGTAACTTGGCAG
TAACTGTGGATGACAGTGCAGAATTTAAATGTGAGGCCCGAGGTGACCCTGTACCTACAGTACGATGGAG
GAAAGATGATGGAGAGCTGCCCAAATCCAGATATGAAATCCGAGATGATCATACCTTGAAAATTAGGAAG
GTGACAGCTGGTGACATGGGTTCATACACTTGTGTTGCAGAAAATATGGTGGGCAAAGCTGAAGCATCTG
CTACTCTGACTGTTCAAGTTGGGTCTGAACCTCCACATTTTGTTGTGAAACCCCGTGACCAGGTTGTTGC
TTTGGGACGGACTGTAACTTTTCAGTGTGAAGCAACCGGAAATCCTCAACCAGCTATTTTCTGGAGGAGA
GAAGGGAGTCAGAATCTACTTTTCTCATATCAACCACCACAGTCATCCAGCCGATTTTCAGTCTCCCAGA
CTGGCGACCTCACAATTACTAATGTCCAGCGATCTGATGTTGGTTATTACATCTGCCAGACTTTAAATGT
TGCTGGAAGCATCATCACAAAGGCATATTTGGAAGTTACAGATGTGATTGCAGATCGGCCTCCCCCAGTT
ATTCGACAAGGTCCTGTGAATCAGACTGTAGCCGTGGATGGCACTTTCGTCCTCAGCTGTGTGGCCACAG
GCAGTCCAGTGCCCACCATTCTGTGGAGAAAGGATGGAGTCCTCGTTTCAACCCAAGACTCTCGAATCAA
ACAGTTGGAGAATGGAGTACTGCAGATCCGATATGCTAAGCTGGGTGATACTGGTCGGTACACCTGCATT
GCATCAACCCCCAGTGGTGAAGCAACATGGAGTGCTTACATTGAAGTTCAAGAATTTGGAGTTCCAGTTC
AGCCTCCAAGACCTACTGACCCAAATTTAATCCCTAGTGCCCCATCAAAACCTGAAGTGACAGATGTCAG
CAGAAATACAGTCACATTATCGTGGCAACCAAATTTGAATTCAGGAGCAACTCCAACATCTTATATTATA
GAAGCCTTCAGCCATGCATCTGGTAGCAGCTGGCAGACCGTAGCAGAGAATGTGAAAACAGAAACATCTG
CCATTAAAGGACTCAAACCTAATGCAATTTACCTTTTCCTTGTGAGGGCAGCTAATGCATATGGAATTAG
TGATCCAAGCCAAATATCAGATCCAGTGAAAACACAAGATGTCCTACCAACAAGTCAGGGGGTGGACCAC
AAGCAGGTCCAGAGAGAGCTGGGAAATGCTGTTCTGCACCTCCACAACCCCACCGTCCTTTCTTCCTCTT
CCATCGAAGTGCACTGGACAGTAGATCAACAGTCTCAGTATATACAAGGATATAAAATTCTCTATCGGCC
ATCTGGAGCCAACCACGGAGAATCAGACTGGTTAGTTTTTGAAGTGAGGACGCCAGCCAAAAACAGTGTG
GTAATCCCTGATCTCAGAAAGGGAGTCAACTATGAAATTAAGGCTCGCCCTTTTTTTAATGAATTTCAAG
GAGCAGATAGTGAAATCAAGTTTGCCAAAACCCTGGAAGAAGCACCCAGTGCCCCACCCCAAGGTGTAAC
TGTATCCAAGAATGATGGAAACGGAACTGCAATTCTAGTTAGTTGGCAGCCACCTCCAGAAGACACTCAA
AATGGAATGGTCCAAGAGTATAAGGTTTGGTGTCTGGGCAATGAAACTCGATACCACATCAACAAAACAG
TGGATGGTTCCACCTTTTCCGTGGTCATTCCCTTTCTTGTTCCTGGAATCCGATACAGTGTGGAAGTGGC
AGCCAGCACTGGGGCTGGGTCTGGGGTAAAGAGTGAGCCTCAGTTCATCCAGCTGGATGCCCATGGAAAC
CCTGTGTCACCTGAGGACCAAGTCAGCCTCGCTCAGCAGATTTCAGATGTGGTGAAGCAGCCGGCCTTCA
TAGCAGGTATTGGAGCAGCCTGTTGGATCATCCTCATGGTCTTCAGCATCTGGCTTTATCGACACCGCAA
GAAGAGAAACGGACTTACTAGTACCTACGCGGGTATCAGAAAAGTCCCGTCTTTTACCTTCACACCAACA
GTAACTTACCAGAGAGGAGGCGAAGCTGTCAGCAGTGGAGGGAGGCCTGGACTTCTCAACATCAGTGAAC
CTGCCGCGCAGCCATGGCTGGCAGACACGTGGCCTAATACTGGCAACAACCACAATGACTGCTCCATCAG
CTGCTGCACGGCAGGCAATGGAAACAGCGACAGCAACCTCACTACCTACAGTCGCCCAGCTGATTGTATA
GCAAATTATAACAACCAACTGGATAACAAACAAACAAATCTGATGCTCCCTGAGTCAACTGTTTATGGTG
ATGTGGACCTTAGTAACAAAATCAATGAGATGAAAACCTTCAATAGCCCAAATCTGAAGGATGGGCGTTT
TGTCAATCCATCAGGGCAGCCTACTCCTTACGCCACCACTCAGCTCATCCAGTCAAACCTCAGCAACAAC
ATGAACAATGGCAGCGGGGACTCTGGCGAGAAGCACTGGAAACCACTGGGACAGCAGAAACAAGAAGTGG
CACCAGTTCAGTACAACATCGTGGAGCAAAACAAGCTGAACAAAGATTATCGAGCAAATGACACAGTTCC
TCCAACTATCCCATACAACCAATCATACGACCAGAACACAGGAGGATCCTACAACAGCTCAGACCGGGGC
AGTAGTACATCTGGGAGTCAGGGGCACAAGAAAGGGGCAAGAACACCCAAGGTACCAAAACAGGGTGGCA
TGAACTGGGCAGACCTGCTTCCTCCTCCCCCAGCACATCCTCCTCCACACAGCAATAGCGAAGAGTACAA
CATTTCTGTAGATGAAAGCTATGACCAAGAAATGCCATGTCCCGTGCCACCAGCAAGGATGTATTTGCAA
CAAGATGAATTAGAAGAGGAGGAAGATGAACGAGGCCCCACTCCCCCTGTTCGGGGAGCAGCTTCTTCTC
CAGCTGCCGTGTCCTATAGCCATCAGTCCACTGCCACTCTGACTCCCTCCCCACAGGAAGAACTCCAGCC
CATGTTACAGGATTGTCCAGAGGAGACTGGCCACATGCAGCACCAGCCCGACAGGAGACGGCAGCCTGTG
AGTCCTCCTCCACCACCACGGCCGATCTCCCCTCCACATACCTATGGCTACATTTCAGGACCCCTGGTCT
CAGATATGGATACGGATGCGCCAGAAGAGGAAGAAGACGAAGCCGACATGGAGGTAGCCAAGATGCAAAC
CAGAAGGCTTTTGTTACGTGGGCTTGAGCAGACACCTGCCTCCAGTGTTGGGGACCTGGAGAGCTCTGTC
ACGGGGTCCATGATCAACGGCTGGGGCTCAGCCTCAGAGGAGGACAACATTTCCAGCGGACGCTCCAGTG
TTAGTTCTTCGGACGGCTCCTTTTTCACTGATGCTGACTTTGCCCAGGCAGTCGCAGCAGCGGCAGAGTA
TGCTGGTCTGAAAGTAGCACGACGGCAAATGCAGGATGCTGCTGGCCGTCGACATTTTCATGCGTCTCAG
TGCCCTAGGCCCACAAGTCCCGTGTCTACAGACAGCAACATGAGTGCCGCCGTAATGCAGAAAACCAGAC
CAGCCAAGAAACTGAAACACCAGCCAGGACATCTGCGCAGAGAAACCTACACAGATGATCTTCCACCACC
TCCTGTGCCGCCACCTGCTATAAAGTCACCTACTGCCCAATCCAAGACACAGCTGGAAGTACGACCTGTA
GTGGTGCCAAAACTCCCTTCTATGGATGCAAGAACAGACAGATCATCAGACAGAAAAGGAAGCAGTTACA
AGGGGAGAGAAGTGTTGGATGGAAGACAGGTTGTTGACATGCGAACAAATCCAGGTGATCCCAGAGAAGC
ACAGGAACAGCAAAATGACGGGAAAGGACGTGGAAACAAGGCAGCAAAACGAGACCTTCCACCAGCAAAG
ACTCATCTCATCCAAGAGGATATTCTACCTTATTGTAGACCTACTTTTCCAACATCAAATAATCCCAGAG
ATCCCAGTTCCTCAAGCTCAATGTCATCAAGAGGATCAGGAAGCAGACAAAGAGAACAAGCAAATGTAGG
TCGAAGAAATATTGCAGAAATGCAGGTACTTGGAGGATATGAAAGAGGAGAAGATAATAATGAAGAATTA
GAGGAAACTGAAAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211961 representing NM_133631
Red=Cloning site Green=Tags(s)

MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVE
TDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDV
MVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGER
ESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRK
VTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRR
EGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPV
IRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCI
ASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYII
EAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDH
KQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSV
VIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQ
NGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGN
PVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPT
VTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCI
ANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNN
MNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRG
SSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQ
QDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPV
SPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSV
TGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQ
CPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPV
VVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAK
THLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEEL
EETES

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_133631
ORF Size 4845 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_133631.1, NM_133631.2, NP_598334.1
RefSeq Size 7475
RefSeq ORF 4821
Locus ID 6091
Cytogenetics 3p12.3
Domains ig, FN3
Protein Families Druggable Genome
Protein Pathways Axon guidance
MW 175.7 kDa
Gene Summary Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Citations (1)

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68 Mouse Clones