ROBO1 (NM_133631) Human Tagged ORF Clone

CAT#: RC211961

ROBO1 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 2

Product Images

Circular map for RC211961

• 

  Western blot validation of overexpression lysate (Cat# LY408772) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211961 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ROBO1
• Synonyms: DUTT1; SAX3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211961 representing NM_133631
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGCGGAGCCCGCTCACTTTTACCTGTTTGGATTAATATGTCTCTGTTCAGGCTCCCGTCTTCGTC
  AGGAAGATTTTCCACCTCGCATTGTTGAACACCCTTCAGACCTGATTGTCTCAAAAGGAGAACCTGCAAC
  TTTGAACTGCAAAGCTGAAGGCCGCCCCACACCCACTATTGAATGGTACAAAGGGGGAGAGAGAGTGGAG
  ACAGACAAAGATGACCCTCGCTCACACCGAATGTTGCTGCCGAGTGGATCTTTATTTTTCTTACGTATAG
  TACATGGACGGAAAAGTAGACCTGATGAAGGAGTCTATGTCTGTGTAGCAAGGAATTACCTTGGAGAGGC
  TGTGAGCCACAATGCATCGCTGGAAGTAGCCATACTTCGGGATGACTTCAGACAAAACCCTTCGGATGTC
  ATGGTTGCAGTAGGAGAGCCTGCAGTAATGGAATGCCAACCTCCACGAGGCCATCCTGAGCCCACCATTT
  CATGGAAGAAAGATGGCTCTCCACTGGATGATAAAGATGAAAGAATAACTATACGAGGAGGAAAGCTCAT
  GATCACTTACACCCGTAAAAGTGACGCTGGCAAATATGTTTGTGTTGGTACCAATATGGTTGGGGAACGT
  GAGAGTGAAGTAGCCGAGCTGACTGTCTTAGAGAGACCATCATTTGTGAAGAGACCCAGTAACTTGGCAG
  TAACTGTGGATGACAGTGCAGAATTTAAATGTGAGGCCCGAGGTGACCCTGTACCTACAGTACGATGGAG
  GAAAGATGATGGAGAGCTGCCCAAATCCAGATATGAAATCCGAGATGATCATACCTTGAAAATTAGGAAG
  GTGACAGCTGGTGACATGGGTTCATACACTTGTGTTGCAGAAAATATGGTGGGCAAAGCTGAAGCATCTG
  CTACTCTGACTGTTCAAGTTGGGTCTGAACCTCCACATTTTGTTGTGAAACCCCGTGACCAGGTTGTTGC
  TTTGGGACGGACTGTAACTTTTCAGTGTGAAGCAACCGGAAATCCTCAACCAGCTATTTTCTGGAGGAGA
  GAAGGGAGTCAGAATCTACTTTTCTCATATCAACCACCACAGTCATCCAGCCGATTTTCAGTCTCCCAGA
  CTGGCGACCTCACAATTACTAATGTCCAGCGATCTGATGTTGGTTATTACATCTGCCAGACTTTAAATGT
  TGCTGGAAGCATCATCACAAAGGCATATTTGGAAGTTACAGATGTGATTGCAGATCGGCCTCCCCCAGTT
  ATTCGACAAGGTCCTGTGAATCAGACTGTAGCCGTGGATGGCACTTTCGTCCTCAGCTGTGTGGCCACAG
  GCAGTCCAGTGCCCACCATTCTGTGGAGAAAGGATGGAGTCCTCGTTTCAACCCAAGACTCTCGAATCAA
  ACAGTTGGAGAATGGAGTACTGCAGATCCGATATGCTAAGCTGGGTGATACTGGTCGGTACACCTGCATT
  GCATCAACCCCCAGTGGTGAAGCAACATGGAGTGCTTACATTGAAGTTCAAGAATTTGGAGTTCCAGTTC
  AGCCTCCAAGACCTACTGACCCAAATTTAATCCCTAGTGCCCCATCAAAACCTGAAGTGACAGATGTCAG
  CAGAAATACAGTCACATTATCGTGGCAACCAAATTTGAATTCAGGAGCAACTCCAACATCTTATATTATA
  GAAGCCTTCAGCCATGCATCTGGTAGCAGCTGGCAGACCGTAGCAGAGAATGTGAAAACAGAAACATCTG
  CCATTAAAGGACTCAAACCTAATGCAATTTACCTTTTCCTTGTGAGGGCAGCTAATGCATATGGAATTAG
  TGATCCAAGCCAAATATCAGATCCAGTGAAAACACAAGATGTCCTACCAACAAGTCAGGGGGTGGACCAC
  AAGCAGGTCCAGAGAGAGCTGGGAAATGCTGTTCTGCACCTCCACAACCCCACCGTCCTTTCTTCCTCTT
  CCATCGAAGTGCACTGGACAGTAGATCAACAGTCTCAGTATATACAAGGATATAAAATTCTCTATCGGCC
  ATCTGGAGCCAACCACGGAGAATCAGACTGGTTAGTTTTTGAAGTGAGGACGCCAGCCAAAAACAGTGTG
  GTAATCCCTGATCTCAGAAAGGGAGTCAACTATGAAATTAAGGCTCGCCCTTTTTTTAATGAATTTCAAG
  GAGCAGATAGTGAAATCAAGTTTGCCAAAACCCTGGAAGAAGCACCCAGTGCCCCACCCCAAGGTGTAAC
  TGTATCCAAGAATGATGGAAACGGAACTGCAATTCTAGTTAGTTGGCAGCCACCTCCAGAAGACACTCAA
  AATGGAATGGTCCAAGAGTATAAGGTTTGGTGTCTGGGCAATGAAACTCGATACCACATCAACAAAACAG
  TGGATGGTTCCACCTTTTCCGTGGTCATTCCCTTTCTTGTTCCTGGAATCCGATACAGTGTGGAAGTGGC
  AGCCAGCACTGGGGCTGGGTCTGGGGTAAAGAGTGAGCCTCAGTTCATCCAGCTGGATGCCCATGGAAAC
  CCTGTGTCACCTGAGGACCAAGTCAGCCTCGCTCAGCAGATTTCAGATGTGGTGAAGCAGCCGGCCTTCA
  TAGCAGGTATTGGAGCAGCCTGTTGGATCATCCTCATGGTCTTCAGCATCTGGCTTTATCGACACCGCAA
  GAAGAGAAACGGACTTACTAGTACCTACGCGGGTATCAGAAAAGTCCCGTCTTTTACCTTCACACCAACA
  GTAACTTACCAGAGAGGAGGCGAAGCTGTCAGCAGTGGAGGGAGGCCTGGACTTCTCAACATCAGTGAAC
  CTGCCGCGCAGCCATGGCTGGCAGACACGTGGCCTAATACTGGCAACAACCACAATGACTGCTCCATCAG
  CTGCTGCACGGCAGGCAATGGAAACAGCGACAGCAACCTCACTACCTACAGTCGCCCAGCTGATTGTATA
  GCAAATTATAACAACCAACTGGATAACAAACAAACAAATCTGATGCTCCCTGAGTCAACTGTTTATGGTG
  ATGTGGACCTTAGTAACAAAATCAATGAGATGAAAACCTTCAATAGCCCAAATCTGAAGGATGGGCGTTT
  TGTCAATCCATCAGGGCAGCCTACTCCTTACGCCACCACTCAGCTCATCCAGTCAAACCTCAGCAACAAC
  ATGAACAATGGCAGCGGGGACTCTGGCGAGAAGCACTGGAAACCACTGGGACAGCAGAAACAAGAAGTGG
  CACCAGTTCAGTACAACATCGTGGAGCAAAACAAGCTGAACAAAGATTATCGAGCAAATGACACAGTTCC
  TCCAACTATCCCATACAACCAATCATACGACCAGAACACAGGAGGATCCTACAACAGCTCAGACCGGGGC
  AGTAGTACATCTGGGAGTCAGGGGCACAAGAAAGGGGCAAGAACACCCAAGGTACCAAAACAGGGTGGCA
  TGAACTGGGCAGACCTGCTTCCTCCTCCCCCAGCACATCCTCCTCCACACAGCAATAGCGAAGAGTACAA
  CATTTCTGTAGATGAAAGCTATGACCAAGAAATGCCATGTCCCGTGCCACCAGCAAGGATGTATTTGCAA
  CAAGATGAATTAGAAGAGGAGGAAGATGAACGAGGCCCCACTCCCCCTGTTCGGGGAGCAGCTTCTTCTC
  CAGCTGCCGTGTCCTATAGCCATCAGTCCACTGCCACTCTGACTCCCTCCCCACAGGAAGAACTCCAGCC
  CATGTTACAGGATTGTCCAGAGGAGACTGGCCACATGCAGCACCAGCCCGACAGGAGACGGCAGCCTGTG
  AGTCCTCCTCCACCACCACGGCCGATCTCCCCTCCACATACCTATGGCTACATTTCAGGACCCCTGGTCT
  CAGATATGGATACGGATGCGCCAGAAGAGGAAGAAGACGAAGCCGACATGGAGGTAGCCAAGATGCAAAC
  CAGAAGGCTTTTGTTACGTGGGCTTGAGCAGACACCTGCCTCCAGTGTTGGGGACCTGGAGAGCTCTGTC
  ACGGGGTCCATGATCAACGGCTGGGGCTCAGCCTCAGAGGAGGACAACATTTCCAGCGGACGCTCCAGTG
  TTAGTTCTTCGGACGGCTCCTTTTTCACTGATGCTGACTTTGCCCAGGCAGTCGCAGCAGCGGCAGAGTA
  TGCTGGTCTGAAAGTAGCACGACGGCAAATGCAGGATGCTGCTGGCCGTCGACATTTTCATGCGTCTCAG
  TGCCCTAGGCCCACAAGTCCCGTGTCTACAGACAGCAACATGAGTGCCGCCGTAATGCAGAAAACCAGAC
  CAGCCAAGAAACTGAAACACCAGCCAGGACATCTGCGCAGAGAAACCTACACAGATGATCTTCCACCACC
  TCCTGTGCCGCCACCTGCTATAAAGTCACCTACTGCCCAATCCAAGACACAGCTGGAAGTACGACCTGTA
  GTGGTGCCAAAACTCCCTTCTATGGATGCAAGAACAGACAGATCATCAGACAGAAAAGGAAGCAGTTACA
  AGGGGAGAGAAGTGTTGGATGGAAGACAGGTTGTTGACATGCGAACAAATCCAGGTGATCCCAGAGAAGC
  ACAGGAACAGCAAAATGACGGGAAAGGACGTGGAAACAAGGCAGCAAAACGAGACCTTCCACCAGCAAAG
  ACTCATCTCATCCAAGAGGATATTCTACCTTATTGTAGACCTACTTTTCCAACATCAAATAATCCCAGAG
  ATCCCAGTTCCTCAAGCTCAATGTCATCAAGAGGATCAGGAAGCAGACAAAGAGAACAAGCAAATGTAGG
  TCGAAGAAATATTGCAGAAATGCAGGTACTTGGAGGATATGAAAGAGGAGAAGATAATAATGAAGAATTA
  GAGGAAACTGAAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211961 representing NM_133631
  Red=Cloning site Green=Tags(s)
  
  MIAEPAHFYLFGLICLCSGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVE
  TDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDV
  MVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGER
  ESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRK
  VTAGDMGSYTCVAENMVGKAEASATLTVQVGSEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRR
  EGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPV
  IRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCI
  ASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYII
  EAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDH
  KQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSV
  VIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQ
  NGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGN
  PVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPT
  VTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCI
  ANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNN
  MNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRG
  SSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQ
  QDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPV
  SPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSV
  TGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQ
  CPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPV
  VVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAK
  THLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEEL
  EETES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133631
• ORF Size: 4845 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133631.2
• RefSeq Size: 7475 bp
• RefSeq ORF: 4821 bp
• Locus ID: 6091
• UniProt ID: Q9Y6N7
• Cytogenetics: 3p12.3
• Domains: ig, FN3
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• MW: 175.7 kDa
• Gene Summary: Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]