IARS2 (NM_018060) Human Tagged ORF Clone

CAT#: RC211919

IARS2 (Myc-DDK-tagged)-Human isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC211919

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IARS2
• Synonyms: CAGSSS; ILERS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211919 representing NM_018060
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTTGGGGGCTGCGCCCTCGCGGGCCGGGCGCGGCCGCCCTGGCCACTGCCCGAAGTTTGTGGGGGA
  CGCCCCGCCTTCCCTGCAGCCCGGGATGGCAAGGGGCGACGAAGAGGCTTCTGGTGCGGTCGGTCTCCGG
  GGCCAGTAACCACCAGCCGAACTCGAATAGTGGCAGATACCGGGACACGGTGCTGCTGCCGCAGACGAGC
  TTCCCCATGAAGCTGCTGGGCCGCCAGCAGCCGGACACGGAGCTGGAGATCCAGCAGAAATGTGGATTTT
  CAGAACTTTATTCATGGCAAAGAGAAAGAAAAGTAAAGACAGAATTTTGCCTTCATGATGGACCTCCTTA
  TGCAAACGGTGACCCTCATGTTGGACATGCTTTAAATAAGATTTTGAAAGACATAGCCAATCGATTCCAT
  ATGATGAATGGCTCCAAAATACATTTTGTGCCCGGCTGGGATTGTCATGGGTTGCCCATTGAAATAAAAG
  TATTATCAGAACTTGGTAGAGAAGCTCAGAATCTTTCAGCTATGGAAATTAGAAAGAAAGCTAGATCATT
  TGCTAAAGCAGCCATTGAGAAACAGAAATCAGCATTTATTCGTTGGGGAATAATGGCAGATTGGAATAAT
  TGCTACTATACATTTGATGGGAAGTATGAAGCCAAACAGTTGAGAACTTTTTACCAAATGTATGATAAGG
  GCTTGGTTTATCGATCTTACAAACCTGTGTTTTGGTCTCCGTCATCTAGGACTGCATTGGCTGAAGCAGA
  ACTTGAATATAATCCTGAGCATGTCAGTCGTTCAATATATGTAAAATTTCCTCTCTTAAAGCCTTCTCCA
  AAATTGGCATCTCTTATAGATGGTTCATCTCCTGTTAGTATTTTGGTCTGGACCACACAACCTTGGACGA
  TTCCAGCCAATGAAGCTGTTTGCTATATGCCTGAATCAAAGTATGCTGTTGTGAAATGTTCTAAGTCTGG
  AGACCTCTACGTACTGGCGGCAGATAAAGTAGCATCTGTTGCTTCTACTTTGGAAACAACATTTGAGACT
  ATTTCAACACTTTCAGGTGTAGATTTGGAAAATGGTACTTGCAGTCATCCATTAATTCCTGATAAAGCCT
  CTCCTCTTTTACCTGCAAATCATGTGACCATGGCAAAAGGAACGGGATTGGTTCACACAGCCCCAGCTCA
  TGGTATGGAAGACTACGGTGTAGCGTCTCAGCACAACCTGCCCATGGATTGTCTAGTGGACGAAGATGGA
  GTTTTCACAGATGTTGCAGGTCCTGAACTTCAAAACAAGGCTGTCCTTGAAGAGGGAACTGATGTGGTTA
  TAAAGATGCTTCAGACTGCAAAGAATTTGTTGAAAGAGGAGAAATTGGTGCATAGCTATCCGTATGACTG
  GAGGACCAAGAAACCTGTGGTTATTCGTGCCAGCAAGCAGTGGTTTATAAACATCACGGATATTAAGACT
  GCAGCCAAGGAATTGTTAAAAAAGGTGAAATTTATTCCTGGATCAGCACTGAATGGCATGGTTGAAATGA
  TGGACAGGCGGCCATATTGGTGTATATCAAGGCAAAGAGTTTGGGGTGTTCCAATTCCTGTGTTTCATCA
  TAAGACCAAGGATGAATACTTGATCAACAGCCAAACCACTGAGCATATTGTTAAACTAGTGGAACAACAC
  GGCAGTGATATCTGGTGGACTCTTCCCCCTGAACAACTTCTTCCAAAAGAAGTCTTATCTGAGGTTGGTG
  GCCCTGATGCCTTGGAATATGTGCCAGGTCAGGATATTTTGGACATCTGGTTTGATAGCGGAACTTCATG
  GTCTTATGTTCTTCCAGGTCCTGACCAAAGAGCAGATTTGTACTTGGAAGGAAAAGACCAGCTCGGGGGT
  TGGTTTCAGTCATCCTTATTAACAAGTGTGGCAGCAAGGAAGAGAGCACCTTATAAGACAGTGATTGTTC
  ATGGATTTACCCTTGGAGAAAAGGGAGAAAAGATGTCCAAGTCTCTTGGGAATGTCATTCATCCTGATGT
  TGTCGTTAATGGAGGACAAGATCAAAGCAAAGAGCCTCCGTATGGTGCTGATGTCCTTTGCTGGTGGGTA
  GCTGATTCCAATGTCTTCACCGAAGTTGCAATTGGCCCATCCGTGCTCAATGCTGCCAGAGATGATATTA
  GCAAGCTTAGGAATACACTTCGCTTTCTTTTGGGAAATGTGGCTGATTTCAACCCAGAAACAGATTCCAT
  CCCTGTAAACGATATGTATGTCATAGACCAGTACATGCTACACTTACTGCAGGATTTGGCAAACAAGATT
  ACCGAATTATACAAACAATATGATTTTGGAAAAGTTGTTCGGCTGTTACGGACGTTTTATACCAGAGAGC
  TCTCTAACTTTTATTTCAGTATAATCAAAGATAGGCTCTATTGTGAAAAGGAAAATGACCCCAAACGACG
  CTCTTGTCAGACTGCATTAGTTGAAATTTTGGATGTAATAGTTCGTTCTTTTGCTCCCATTCTTCCTCAC
  CTGGCTGAAGAGGTGTTCCAGCACATACCTTATATTAAAGAGCCCAAGAGTGTTTTCCGTACTGGGTGGA
  TTAGTACTAGTTCTATCTGGAAAAAGCCCGGGTTGGAAGAAGCTGTGGAGAGTGCGTGTGCAATGCGAGA
  CTCATTTCTTGGAAGCATCCCTGGCAAAAATGCAGCTGAGTACAAGGTTATCACTGTGATAGAACCTGGA
  CTGCTTTTTGAGATAATAGAGATGCTGCAGTCTGAAGAGACTTCCAGCACCTCTCAGTTGAATGAATTAA
  TGATGGCTTCTGAGTCAACTTTACTGGCTCAGGAACCACGAGAGATGACTGCAGATGTAATCGAGCTTAA
  AGGGAAATTCCTCATCAACTTAGAAGGTGGTGATATTCGTGAAGAGTCTTCCTATAAAGTAATTGTCATG
  CCGACTACGAAAGAAAAATGCCCCCGTTGTTGGAAGTATACAGCGGAGTCTTCAGATACACTGTGTCCTC
  GATGTGCAGAAGTTGTCAGTGGAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211919 representing NM_018060
  Red=Cloning site Green=Tags(s)
  
  MRWGLRPRGPGAAALATARSLWGTPRLPCSPGWQGATKRLLVRSVSGASNHQPNSNSGRYRDTVLLPQTS
  FPMKLLGRQQPDTELEIQQKCGFSELYSWQRERKVKTEFCLHDGPPYANGDPHVGHALNKILKDIANRFH
  MMNGSKIHFVPGWDCHGLPIEIKVLSELGREAQNLSAMEIRKKARSFAKAAIEKQKSAFIRWGIMADWNN
  CYYTFDGKYEAKQLRTFYQMYDKGLVYRSYKPVFWSPSSRTALAEAELEYNPEHVSRSIYVKFPLLKPSP
  KLASLIDGSSPVSILVWTTQPWTIPANEAVCYMPESKYAVVKCSKSGDLYVLAADKVASVASTLETTFET
  ISTLSGVDLENGTCSHPLIPDKASPLLPANHVTMAKGTGLVHTAPAHGMEDYGVASQHNLPMDCLVDEDG
  VFTDVAGPELQNKAVLEEGTDVVIKMLQTAKNLLKEEKLVHSYPYDWRTKKPVVIRASKQWFINITDIKT
  AAKELLKKVKFIPGSALNGMVEMMDRRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTTEHIVKLVEQH
  GSDIWWTLPPEQLLPKEVLSEVGGPDALEYVPGQDILDIWFDSGTSWSYVLPGPDQRADLYLEGKDQLGG
  WFQSSLLTSVAARKRAPYKTVIVHGFTLGEKGEKMSKSLGNVIHPDVVVNGGQDQSKEPPYGADVLCWWV
  ADSNVFTEVAIGPSVLNAARDDISKLRNTLRFLLGNVADFNPETDSIPVNDMYVIDQYMLHLLQDLANKI
  TELYKQYDFGKVVRLLRTFYTRELSNFYFSIIKDRLYCEKENDPKRRSCQTALVEILDVIVRSFAPILPH
  LAEEVFQHIPYIKEPKSVFRTGWISTSSIWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYKVITVIEPG
  LLFEIIEMLQSEETSSTSQLNELMMASESTLLAQEPREMTADVIELKGKFLINLEGGDIREESSYKVIVM
  PTTKEKCPRCWKYTAESSDTLCPRCAEVVSGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018060
• ORF Size: 3036 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018060.3, NP_060530.3
• RefSeq Size: 3549 bp
• RefSeq ORF: 3039 bp
• Locus ID: 55699
• UniProt ID: Q9NSE4
• Cytogenetics: 1q41
• Domains: tRNA-synt_1
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis
• MW: 113.6 kDa
• Gene Summary: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Dec 2014]