RBPSUHL (RBPJL) (NM_014276) Human Tagged ORF Clone

CAT#: RC211868

RBPJL (Myc-DDK-tagged)-Human recombination signal binding protein for immunoglobulin kappa J region-like (RBPJL)

Product Images

Circular map for RC211868

• 

  Western blot validation of overexpression lysate (Cat# LY415401) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211868 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RBPSUHL
• Synonyms: RBPL; RBPSUHL; SUHL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211868 representing NM_014276
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCCGCAGGGGCAGCAGACCCCTCAGTGCCTCCCAATCCTTTGACTCACCTGAGCCTGCAGGACA
  GATCAGAGATGCAGCTGCAGAGCGAAGCCGACAGGCGGAGCCTCCCGGGCACTTGGACCAGGTCATCCCC
  AGAGCACACCACCATTCTGAGGGGAGGCGTGCGCAGGTGCCTGCAGCAACAGTGTGAACAGACTGTGCGG
  ATCCTGCATGCCAAGGTGGCCCAGAAATCATACGGAAATGAGAAGCGGTTCTTCTGCCCCCCGCCCTGTG
  TCTACCTCTCGGGGCCTGGCTGGAGGGTGAAGCCAGGGCAGGATCAAGCTCACCAGGCGGGGGAAACGGG
  GCCCACGGTCTGCGGTTACATGGGACTGGACAGCGCGTCCGGCAGCGCCACTGAGACGCAGAAGCTGAAT
  TTCGAGCAGCAGCCGGACTCCAGGGAATTCGGCTGCGCCAAGACCCTGTACATCTCAGATGCAGACAAGA
  GGAAGCACTTTCGGCTGGTGCTGCGGCTGGTGCTGCGCGGGGGCCGGGAGCTGGGTACCTTCCACAGCCG
  CCTTATCAAGGTCATCTCGAAGCCCTCGCAGAAGAAGCAGTCGCTGAAAAACACCGATCTGTGCATATCC
  TCCGGCTCAAAGGTCTCCCTCTTCAACCGCCTGCGCTCTCAGACGGTCTCCACACGCTACCTCTCTGTGG
  AGGATGGGGCCTTTGTGGCCAGTGCACGACAGTGGGCTGCCTTCACGCTCCACCTGGCTGATGGGCACTC
  TGCCCAAGGAGACTTCCCACCGCGAGAGGGCTACGTTCGCTATGGCTCCCTGGTGCAGCTCGTCTGCACG
  GTCACCGGCATCACACTACCTCCCATGATCATCCGTAAAGTAGCAAAACAGTGTGCGCTCCTTGATGTGG
  ATGAGCCCATCTCCCAGCTGCACAAGTGTGCATTCCAGTTTCCAGGCAGTCCCCCAGGAGGGGGTGGCAC
  CTACTTATGCCTTGCCACAGAGAAGGTGGTGCAATTTCAGGCCTCTCCCTGCCCCAAGGAGGCGAACAGG
  GCTCTGCTTAACGACAGCTCTTGCTGGACCATCATCGGCACCGAGTCGGTGGAATTTTCCTTCAGCACCA
  GCCTGGCGTGTACCCTGGAGCCGGTCACTCCGGTGCCTCTCATCAGCACCCTAGAGCTGAGCGGCGGGGG
  CGACGTGGCCACGCTGGAGCTCCACGGAGAGAACTTCCACGCGGGGCTCAAGGTGTGGTTTGGGGACGTG
  GAGGCAGAAACCATGTACAGGAGCCCGCGGTCCCTGGTGTGCGTGGTGCCGGACGTGGCGGCCTTCTGCA
  GCGACTGGCGCTGGCTGCGCGCTCCCATCACAATCCCCATGAGCCTGGTGCGCGCCGACGGGCTCTTCTA
  CCCTAGTGCCTTCTCCTTCACCTACACCCCGGAATACAGCGTGCGGCCGGGTCACCCCGGCGTCCCCGAG
  CCCGCCACCGACGCCGACGCGCTCCTGGAGAGCATCCATCAGGAGTTCACGCGCACCAACTTCCACCTCT
  TCATCCAGACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211868 representing NM_014276
  Red=Cloning site Green=Tags(s)
  
  MDPAGAADPSVPPNPLTHLSLQDRSEMQLQSEADRRSLPGTWTRSSPEHTTILRGGVRRCLQQQCEQTVR
  ILHAKVAQKSYGNEKRFFCPPPCVYLSGPGWRVKPGQDQAHQAGETGPTVCGYMGLDSASGSATETQKLN
  FEQQPDSREFGCAKTLYISDADKRKHFRLVLRLVLRGGRELGTFHSRLIKVISKPSQKKQSLKNTDLCIS
  SGSKVSLFNRLRSQTVSTRYLSVEDGAFVASARQWAAFTLHLADGHSAQGDFPPREGYVRYGSLVQLVCT
  VTGITLPPMIIRKVAKQCALLDVDEPISQLHKCAFQFPGSPPGGGGTYLCLATEKVVQFQASPCPKEANR
  ALLNDSSCWTIIGTESVEFSFSTSLACTLEPVTPVPLISTLELSGGGDVATLELHGENFHAGLKVWFGDV
  EAETMYRSPRSLVCVVPDVAAFCSDWRWLRAPITIPMSLVRADGLFYPSAFSFTYTPEYSVRPGHPGVPE
  PATDADALLESIHQEFTRTNFHLFIQT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014276
• ORF Size: 1551 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014276.4
• RefSeq Size: 2630 bp
• RefSeq ORF: 1554 bp
• Locus ID: 11317
• UniProt ID: Q9UBG7
• Cytogenetics: 20q13.12
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Notch signaling pathway
• MW: 56.6 kDa
• Gene Summary: This gene encodes a member of the suppressor of hairless protein family. A similar protein in mouse is a transcription factor that binds to DNA sequences almost identical to that bound by the Notch receptor signaling pathway transcription factor recombining binding protein J. The mouse protein has been shown to activate transcription in concert with Epstein-Barr virus nuclear antigen-2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]