HERC4 (NM_022079) Human Tagged ORF Clone

CAT#: RC211865

HERC4 (Myc-DDK-tagged)-Human hect domain and RLD 4 (HERC4), transcript variant 1

Product Images

Circular map for RC211865

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HERC4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211865 representing NM_022079
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTGCTGGGGAAATGCATCCTTTGGGCAGCTAGGTTTGGGTGGAATTGATGAAGAAATTGTACTAG
  AGCCCAGAAAAAGTGACTTCTTTATAAATAAAAGGGTCCGAGATGTAGGATGTGGACTCAGACATACTGT
  GTTTGTTCTGGATGATGGAACAGTGTACACATGTGGATGTAATGATCTAGGACAGCTAGGTCATGAAAAA
  TCCAGAAAGAAACCAGAGCAGGTTGTTGCCCTGGATGCCCAAAATATTGTAGCTGTTTCATGTGGAGAAG
  CTCATACGTTAGCGCTAAATGACAAAGGCCAGGTGTATGCTTGGGGTCTCGATTCTGATGGACAGCTTGG
  CCTGGTAGGATCAGAGGAATGCATCAGAGTACCCAGAAATATTAAAAGTTTGTCAGATATCCAGATTGTA
  CAGGTTGCTTGTGGTTACTATCATTCACTTGCACTTTCTAAAGCAAGTGAAGTCTTCTGTTGGGGACAGA
  ATAAATATGGCCAATTGGGTTTAGGTACTGACTGTAAAAAGCAAACTTCACCGCAGCTGCTTAAGTCTTT
  GCTTGGAATCCCTTTCATGCAAGTTGCAGCAGGAGGAGCCCATAGTTTTGTACTCACCCTTTCTGGAGCT
  ATCTTTGGATGGGGACGCAACAAGTTTGGTCAGCTAGGTCTTAATGATGAAAATGATAGGTATGTTCCTA
  ATTTACTAAAGTCACTAAGATCTCAGAAAATAGTTTATATTTGTTGTGGAGAAGATCATACTGCTGCTCT
  AACCAAGGAAGGTGGAGTGTTTACTTTTGGAGCTGGAGGGTATGGTCAGTTGGGCCATAATTCTACCAGT
  CATGAAATAAACCCAAGGAAAGTTTTTGAACTTATGGGAAGCATTGTCACTGAGATTGCTTGTGGACGGC
  AGCACACTTCTGCTTTTGTTCCTTCATCAGGACGAATTTACTCTTTTGGGCTTGGTGGTAATGGGCAGCT
  GGGAACCGGTTCAACAAGCAACAGGAAAAGCCCCTTTACTGTAAAAGGAAATTGGTACCCCTATAATGGG
  CAGTGTCTACCAGATATTGATTCTGAAGAATATTTCTGTGTAAAAAGAATTTTCTCAGGGGGAGATCAAA
  GCTTTTCACATTACTCTAGTCCCCAGAACTGTGGGCCACCAGATGACTTCAGATGTCCCAATCCGACAAA
  GCAGATCTGGACAGTGAATGAAGCTCTAATTCAGAAATGGCTGAGCTATCCTTCTGGAAGGTTTCCTGTG
  GAGATAGCCAATGAGATAGATGGAACGTTTTCTTCCTCTGGTTGCCTAAATGGAAGTTTTTTAGCTGTTA
  GCAATGATGATCACTATAGAACAGGTACCAGATTTTCAGGGGTTGATATGAATGCTGCTAGGCTTTTATT
  CCACAAACTTATACAACCTGATCATCCGCAGATATCTCAGCAGGTGGCAGCTAGTTTGGAAAAGAATCTT
  ATTCCTAAACTGACTAGCTCCTTACCTGATGTTGAAGCATTGAGGTTTTATCTTACTCTACCAGAATGTC
  CCCTGATGAGTGATTCCAACAATTTCACAACAATAGCAATTCCCTTTGGTACAGCTCTTGTGAACCTAGA
  AAAGGCACCACTGAAAGTACTTGAAAACTGGTGGTCAGTACTTGAACCTCCACTATTCCTCAAGATAGTA
  GAACTTTTTAAGGAAGTTGTGGTACATCTTTTGAAACTCTACAAGATCGGTATTCCCCCTTCTGAAAGAA
  GAATTTTCAACAGTTTTCTTCATACTGCATTAAAGGTTTTAGAAATACTACATAGGGTAAATGAGAAAAT
  GGGACAGATTATACAGTATGATAAATTTTATATACATGAAGTACAAGAATTGATAGACATAAGAAATGAT
  TATATCAACTGGGTCCAACAGCAGGCCTATGGAATGGATGTCAACCATGGATTAACTGAGTTGGCAGATA
  TCCCTGTTACAATCTGTACATATCCATTTGTATTTGATGCCCAAGCAAAAACTACTCTGTTACAGACCGA
  TGCAGTCTTACAGATGCAGATGGCTATTGATCAGGCCCACAGGCAGAATGTCTCCTCTCTTTTTCTCCCA
  GTGATTGAATCTGTGAATCCCTGCTTAATTCTAGTGGTGCGTAGAGAAAATATTGTAGGAGATGCAATGG
  AAGTCCTTAGGAAAACAAAGAACATAGATTACAAGAAGCCACTCAAGGTTATATTTGTTGGAGAAGATGC
  TGTGGATGCAGGAGGGGTGCGCAAAGAATTTTTCTTGCTCATCATGAGGGAATTATTGGATCCTAAATAC
  GGCATGTTTAGGTATTATGAAGATTCCAGGCTCATTTGGTTTTCTGATAAGACATTTGAAGACAGTGATT
  TGTTCCATTTGATTGGTGTTATCTGTGGCTTAGCAATTTATAATTGTACCATTGTGGACCTCCATTTTCC
  TTTGGCTTTATATAAGAAACTACTGAAAAAGAAGCCATCCTTGGATGATTTGAAAGAACTAATGCCTGAT
  GTTGGGAGAAGCATGCAACAGTTACTGGATTATCCAGAAGATGACATAGAGGAAACATTTTGTCTTAATT
  TTACGATCACAGTTGAAAACTTTGGTGCAACAGAAGTGAAAGAGCTGGTTCTAAATGGTGCAGACACAGC
  TGTTAACAAACAAAATCGGCAAGAGTTTGTCGATGCTTATGTGGATTACATATTCAATAAATCAGTGGCT
  TCCTTATTTGATGCTTTTCATGCGGGCTTTCATAAGGTCTGTGGAGGAAAAGTCCTTCTGCTCTTTCAGC
  CTAATGAACTACAAGCAATGGTCATTGGAAATACAAATTATGATTGGAAGGAACTGGAAAAGAATACAGA
  ATACAAAGGGGAATATTGGGCAGAACATCCTACGATAAAAATTTTTTGGGAAGTATTTCACGAATTACCA
  TTGGAAAAGAAGAAACAGTTTCTGTTATTTTTGACAGGTAGTGATCGCATTCCTATTCTTGGTATGAAGA
  GTCTGAAACTAGTCATCCAGTCCACAGGAGGTGGTGAGGAGTATCTCCCAGTTTCCCATACTTGTTTTAA
  TCTTCTGGATCTTCCAAAATATACAGAAAAAGAAACTCTACGCTCTAAACTGATCCAAGCTATTGATCAC
  AATGAAGGCTTCAGTTTAATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211865 representing NM_022079
  Red=Cloning site Green=Tags(s)
  
  MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKRVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEK
  SRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLVGSEECIRVPRNIKSLSDIQIV
  QVACGYYHSLALSKASEVFCWGQNKYGQLGLGTDCKKQTSPQLLKSLLGIPFMQVAAGGAHSFVLTLSGA
  IFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTS
  HEINPRKVFELMGSIVTEIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWYPYNG
  QCLPDIDSEEYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCPNPTKQIWTVNEALIQKWLSYPSGRFPV
  EIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNL
  IPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIV
  ELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKMGQIIQYDKFYIHEVQELIDIRND
  YINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLP
  VIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKY
  GMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNCTIVDLHFPLALYKKLLKKKPSLDDLKELMPD
  VGRSMQQLLDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVA
  SLFDAFHAGFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELP
  LEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDH
  NEGFSLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022079
• ORF Size: 3171 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022079.3
• RefSeq Size: 4445 bp
• RefSeq ORF: 3174 bp
• Locus ID: 26091
• UniProt ID: Q5GLZ8
• Cytogenetics: 10q21.3
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 118.4 kDa
• Gene Summary: HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008]