GAS2L1 (NM_152236) Human Tagged ORF Clone

CAT#: RC211855

GAS2L1 (Myc-DDK-tagged)-Human growth arrest-specific 2 like 1 (GAS2L1), transcript variant 2

Product Images

Circular map for RC211855

• 

  Western blot validation of overexpression lysate (Cat# LY407671) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211855 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GAS2L1
• Synonyms: GAR22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211855 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGACCCAGTGGCGGGCATCGCGGGTTCGGCGGCCAAGAGCGTGCGGCCATTTCGCTCCAGTGAGG
  CCTACGTGGAGGCCATGAAGGAGGACCTGGCCGAGTGGCTCAATGCCTTGTACGGCCTGGGTCTCCCGGG
  TGGTGGCGATGGCTTCCTGACAGGGCTGGCCACGGGCACGACCCTGTGCCAACATGCCAACGCCGTGACC
  GAGGCTGCCCGTGCATTGGCAGCCGCCCGCCCGGCCCGAGGTGTGGCCTTCCAGGCGCACAGTGTAGTGC
  CTGGCTCCTTCATGGCGCGCGACAACGTGGCCACCTTCATCGGCTGGTGCCGCGTGGAGCTGGGTGTGCC
  GGAGGTGCTCATGTTTGAGACTGAGGACCTGGTGCTGCGCAAGAACGAGAAGAGCGTGGTGCTGTGCCTG
  CTGGAGGTGGCGCGGCGTGGGGCACGCCTGGGCCTGCTGGCCCCACGCCTCGTGCAGTTTGAGCAGGAGA
  TTGAGCGGGAGCTGCGTGCTGCACCCCCAGCCCCCAACGCCCCTGCCGCTGGGGAGGACACCACTGAAAC
  CGCCCCCGCACCAGGGACTCCTGCCCGCGGCCCCCGCATGACACCCAGCGACCTGCGCAACCTCGACGAG
  CTGGTGAGGGAGATTCTGGGCCGCTGCACCTGCCCTGACCAGTTTCCCATGATCAAGGTCTCAGAGGGGA
  AGTACCGTGTGGGGGACTCGAGCCTGCTCATCTTTGTGCGGGTGCTGAGGAGCCACGTGATGGTGCGAGT
  GGGTGGTGGCTGGGACACGCTGGAGCATTACCTGGACAAGCACGACCCGTGCCGCTGCTCCTCCACTGCT
  CATCGCCCACCCCAGCCGAGGGTCTGCACCTTTTCTCCACAGAGGGTGTCGCCCACCACCAGTCCCCGCC
  CTGCTAGCCCAGTCCCTGGGAGTGAGCGCCGGGGCTCCCGGCCTGAGATGACTCCCGTTAGCTTACGAAG
  CACAAAGGAGGGGCCCGAGACCCCACCCAGGCCCCGGGATCAGCTGCCCCCCCATCCCCGCTCCCGCCGC
  TACTCCGGGGACAGTGACTCCTCAGCCTCCTCCGCCCAGAGCGGCCCCCTTGGTACCCGCAGTGATGACA
  CAGGCACTGGCCCCCGGAGGGAGCGACCCAGCCGGCGGCTGACCACAGGCACCCCGGCCTCTCCGAGACG
  GCCTCCTGCCCTGCGCAGCCAGTCCCGAGACCGGCTGGATCGCGGCCGGCCCCGGGGGGCCCCAGGAGGC
  AGGGGAGCCCAGCTGTCGGTCCCCAGCCCTGCCCGGCGGGCCCGGAGCCAGAGCCGCGAGGAGCAGGCTG
  TGCTGCTTGTGCGCAGGGATCGAGACGGGCAGCACTCATGGGTGCCAAGGGGCAGGGGCAGTGGGGGCTC
  GGGCAGGAGCACCCCCCAGACTCCCCGTGCCCGCAGCCCTGCAGCACCCCGGCTTTCCCGGGTCTCCAGC
  CCCAGTCCAGAGTTGGGCACCACACCGGCCAGCATCTTCCGCACACCCCTGCAGCTCGACCCGCAGCAGG
  AGCAGCAGCTGTTCCGGCGCCTGGAAGAGGAGTTCCTGGCCAATGCCCGGGCCCTTGAGGCTGTTGCTAG
  CGTGACCCCCACTGGACCAGCCCCTGACCCAGCTCGGGCCCCCGACCCTCCAGCTCCTGACTCTGCCTAT
  TGTTCCTCCAGTTCCTCCTCTTCGTCCCTCAGCGTCCTGGGTGGCAAATGTGGCCAACCTGGGGACTCTG
  GCCGGACGGCCAATGGGCTGCCTGGGCCCCGAAGCCAAGCCCTTTCCAGCTCCTCCGATGAAGGCAGCCC
  CTGCCCTGGCATGGGGGGGCCACTAGATGCACCTGGGAGCCCCCTGGCTTGCACTGAACCCTCGAGGACC
  TGGGCACGGGGTCGGATGGACACACAGCCAGACCGTAAACCCTCACGTATCCCCACGCCTCGGGGCCCCC
  GCCGCCCCTCCGGACCCGCAGAGCTGGGGACATGGCATGCCCTGCACTCAGTCACCCCGAGGGCTGAGCC
  AGATTCCTGGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211855 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADPVAGIAGSAAKSVRPFRSSEAYVEAMKEDLAEWLNALYGLGLPGGGDGFLTGLATGTTLCQHANAVT
  EAARALAAARPARGVAFQAHSVVPGSFMARDNVATFIGWCRVELGVPEVLMFETEDLVLRKNEKSVVLCL
  LEVARRGARLGLLAPRLVQFEQEIERELRAAPPAPNAPAAGEDTTETAPAPGTPARGPRMTPSDLRNLDE
  LVREILGRCTCPDQFPMIKVSEGKYRVGDSSLLIFVRVLRSHVMVRVGGGWDTLEHYLDKHDPCRCSSTA
  HRPPQPRVCTFSPQRVSPTTSPRPASPVPGSERRGSRPEMTPVSLRSTKEGPETPPRPRDQLPPHPRSRR
  YSGDSDSSASSAQSGPLGTRSDDTGTGPRRERPSRRLTTGTPASPRRPPALRSQSRDRLDRGRPRGAPGG
  RGAQLSVPSPARRARSQSREEQAVLLVRRDRDGQHSWVPRGRGSGGSGRSTPQTPRARSPAAPRLSRVSS
  PSPELGTTPASIFRTPLQLDPQQEQQLFRRLEEEFLANARALEAVASVTPTGPAPDPARAPDPPAPDSAY
  CSSSSSSSSLSVLGGKCGQPGDSGRTANGLPGPRSQALSSSSDEGSPCPGMGGPLDAPGSPLACTEPSRT
  WARGRMDTQPDRKPSRIPTPRGPRRPSGPAELGTWHALHSVTPRAEPDSWM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152236
• ORF Size: 2043 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152236.3
• RefSeq Size: 2549 bp
• RefSeq ORF: 2046 bp
• Locus ID: 10634
• UniProt ID: Q99501
• Cytogenetics: 22q12.2
• Domains: CH, GAS2
• MW: 72.7 kDa
• Gene Summary: This gene encodes a member of the growth arrest-specific 2 protein family. This protein binds components of the cytoskeleton and may be involved in mediating interactions between microtubules and microfilaments. This protein localizes to the proximal end of mature centrioles and links centrosomes to both microtubules and actin. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 9. [provided by RefSeq, May 2018]