TBX18 (NM_001080508) Human Tagged ORF Clone

CAT#: RC211838

TBX18 (Myc-DDK-tagged)-Human T-box 18 (TBX18)

Product Images

Circular map for RC211838

• 

  Western blot validation of overexpression lysate (Cat# LY421063) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211838 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TBX18
• Synonyms: CAKUT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211838 representing NM_001080508
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGAAGCGAAGGGGCTCGCCGTGCAGCATGCTAAGCCTCAAGGCGCACGCTTTCTCGGTGGAGG
  CGCTGATCGGCGCCGAGAAGCAGCAACAGCTTCAGAAGAAGCGGCGAAAACTGGGCGCCGAAGAGGCGGC
  GAGGGCCGTGGACGACGGAGGCTGCAGCCGCGGCGGCGGCGCGGGCGAAAAGGGTTCTTCTGAGGGAGAC
  GAAGGCGCTGCGCTCCCGCCGCCGGCTGGGGCGACGTCTGGGCCGGCTCGGAGTGGCGCAGACCTGGAGC
  GCGGAGCCGCGGGCGGCTGTGAGGACGGCTTCCAGCAGGGAGCTTCCCCTCTGGCGTCACCGGGAGGCTC
  CCCCAAGGGGTCTCCGGCGCGCTCCCTGGCCCGGCCCGGGACCCCTCTGCCCTCGCCGCAGGCCCCGCGG
  GTGGATCTGCAGGGAGCCGAGCTCTGGAAGCGCTTTCATGAGATAGGCACTGAGATGATCATCACCAAGG
  CCGGCAGGCGCATGTTTCCAGCAATGAGAGTGAAGATCTCTGGATTAGATCCTCACCAGCAATATTACAT
  TGCCATGGATATTGTACCAGTGGACAACAAAAGATACAGGTATGTTTACCACAGTTCGAAATGGATGGTG
  GCAGGTAATGCTGACTCGCCTGTGCCACCCCGTGTGTACATTCATCCAGACTCGCCTGCCTCGGGGGAGA
  CTTGGATGAGACAAGTTATCAGCTTCGACAAGCTGAAGCTCACCAACAATGAACTGGATGACCAAGGCCA
  TATTATTCTTCATTCTATGCACAAATACCAACCGCGAGTGCACGTCATCCGTAAAGACTGTGGAGACGAT
  CTTTCTCCCATCAAGCCTGTTCCATCCGGGGAGGGAGTAAAGGCATTCTCCTTTCCAGAAACTGTCTTCA
  CAACCGTCACTGCCTATCAGAATCAGCAGATTACTCGCCTGAAGATAGATAGGAATCCATTTGCTAAAGG
  CTTCCGAGACTCCGGGCGCAACAGAATGGGTTTGGAAGCCTTGGTGGAATCATATGCATTCTGGCGACCA
  TCACTACGGACTCTGACCTTTGAAGATATCCCTGGAATTCCCAAGCAAGGCAATGCAAGTTCCTCCACCT
  TGCTCCAAGGTACTGGGAATGGCGTTCCTGCCACTCACCCTCACCTTTTGTCTGGCTCCTCTTGCTCCTC
  TCCTGCCTTCCATCTGGGGCCCAACACCAGCCAGCTGTGTAGTCTGGCCCCTGCTGACTATTCTGCCTGT
  GCCCGCTCAGGCCTCACCCTCAACCGATACAGCACATCTTTGGCAGAGACCTACAACAGGCTCACCAACC
  AGGCTGGTGAGACCTTTGCCCCGCCCAGGACTCCCTCCTATGTGGGCGTGAGCAGCAGCACCTCCGTGAA
  CATGTCCATGGGTGGCACTGATGGGGACACCTTCAGCTGCCCACAGACCAGCTTATCCATGCAGATTTCG
  GGAATGTCCCCCCAGCTCCAGTATATCATGCCATCACCCTCCAGCAATGCCTTCGCCACTAACCAGACCC
  ATCAGGGTTCCTATAATACTTTTAGATTACACAGCCCCTGTGCACTATATGGATATAACTTCTCCACATC
  CCCCAAACTGGCTGCCAGTCCTGAGAAAATTGTTTCTTCCCAAGGAAGTTTCTTGGGGTCCTCACCGAGT
  GGGACCATGACGGATCGGCAGATGTTGCCCCCTGTGGAAGGAGTGCACCTGCTTAGCAGTGGGGGTCAGC
  AGAGTTTCTTTGACTCTAGGACCCTAGGAAGCTTAACTCTGTCATCATCTCAAGTATCTGCACATATGGT
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211838 representing NM_001080508
  Red=Cloning site Green=Tags(s)
  
  MAEKRRGSPCSMLSLKAHAFSVEALIGAEKQQQLQKKRRKLGAEEAARAVDDGGCSRGGGAGEKGSSEGD
  EGAALPPPAGATSGPARSGADLERGAAGGCEDGFQQGASPLASPGGSPKGSPARSLARPGTPLPSPQAPR
  VDLQGAELWKRFHEIGTEMIITKAGRRMFPAMRVKISGLDPHQQYYIAMDIVPVDNKRYRYVYHSSKWMV
  AGNADSPVPPRVYIHPDSPASGETWMRQVISFDKLKLTNNELDDQGHIILHSMHKYQPRVHVIRKDCGDD
  LSPIKPVPSGEGVKAFSFPETVFTTVTAYQNQQITRLKIDRNPFAKGFRDSGRNRMGLEALVESYAFWRP
  SLRTLTFEDIPGIPKQGNASSSTLLQGTGNGVPATHPHLLSGSSCSSPAFHLGPNTSQLCSLAPADYSAC
  ARSGLTLNRYSTSLAETYNRLTNQAGETFAPPRTPSYVGVSSSTSVNMSMGGTDGDTFSCPQTSLSMQIS
  GMSPQLQYIMPSPSSNAFATNQTHQGSYNTFRLHSPCALYGYNFSTSPKLAASPEKIVSSQGSFLGSSPS
  GTMTDRQMLPPVEGVHLLSSGGQQSFFDSRTLGSLTLSSSQVSAHMV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080508
• ORF Size: 1821 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080508.3
• RefSeq Size: 4070 bp
• RefSeq ORF: 1824 bp
• Locus ID: 9096
• UniProt ID: O95935
• Cytogenetics: 6q14.3
• MW: 64.6 kDa
• Gene Summary: This genes codes for a member of an evolutionarily conserved family of transcription factors that plays a crucial role in embryonic development. The family is characterized by the presence of the DNA-binding T-box domain and is divided into five sub-families based on sequence conservation in this domain. The encoded protein belongs to the vertebrate specific Tbx1 sub-family. The protein acts as a transcriptional repressor by antagonizing transcriptional activators in the T-box family. The protein forms homo- or heterodimers with other transcription factors of the T-box family or other transcription factors. [provided by RefSeq, Nov 2012]