TBX18 (NM_001080508) Human Tagged ORF Clone

CAT#: RC211838

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TBX18 (Myc-DDK-tagged)-Human T-box 18 (TBX18)



Reconstitution Protocol

USD 565.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TBX18
Synonyms CAKUT2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211838 representing NM_001080508
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAGAAGCGAAGGGGCTCGCCGTGCAGCATGCTAAGCCTCAAGGCGCACGCTTTCTCGGTGGAGG
CGCTGATCGGCGCCGAGAAGCAGCAACAGCTTCAGAAGAAGCGGCGAAAACTGGGCGCCGAAGAGGCGGC
GAGGGCCGTGGACGACGGAGGCTGCAGCCGCGGCGGCGGCGCGGGCGAAAAGGGTTCTTCTGAGGGAGAC
GAAGGCGCTGCGCTCCCGCCGCCGGCTGGGGCGACGTCTGGGCCGGCTCGGAGTGGCGCAGACCTGGAGC
GCGGAGCCGCGGGCGGCTGTGAGGACGGCTTCCAGCAGGGAGCTTCCCCTCTGGCGTCACCGGGAGGCTC
CCCCAAGGGGTCTCCGGCGCGCTCCCTGGCCCGGCCCGGGACCCCTCTGCCCTCGCCGCAGGCCCCGCGG
GTGGATCTGCAGGGAGCCGAGCTCTGGAAGCGCTTTCATGAGATAGGCACTGAGATGATCATCACCAAGG
CCGGCAGGCGCATGTTTCCAGCAATGAGAGTGAAGATCTCTGGATTAGATCCTCACCAGCAATATTACAT
TGCCATGGATATTGTACCAGTGGACAACAAAAGATACAGGTATGTTTACCACAGTTCGAAATGGATGGTG
GCAGGTAATGCTGACTCGCCTGTGCCACCCCGTGTGTACATTCATCCAGACTCGCCTGCCTCGGGGGAGA
CTTGGATGAGACAAGTTATCAGCTTCGACAAGCTGAAGCTCACCAACAATGAACTGGATGACCAAGGCCA
TATTATTCTTCATTCTATGCACAAATACCAACCGCGAGTGCACGTCATCCGTAAAGACTGTGGAGACGAT
CTTTCTCCCATCAAGCCTGTTCCATCCGGGGAGGGAGTAAAGGCATTCTCCTTTCCAGAAACTGTCTTCA
CAACCGTCACTGCCTATCAGAATCAGCAGATTACTCGCCTGAAGATAGATAGGAATCCATTTGCTAAAGG
CTTCCGAGACTCCGGGCGCAACAGAATGGGTTTGGAAGCCTTGGTGGAATCATATGCATTCTGGCGACCA
TCACTACGGACTCTGACCTTTGAAGATATCCCTGGAATTCCCAAGCAAGGCAATGCAAGTTCCTCCACCT
TGCTCCAAGGTACTGGGAATGGCGTTCCTGCCACTCACCCTCACCTTTTGTCTGGCTCCTCTTGCTCCTC
TCCTGCCTTCCATCTGGGGCCCAACACCAGCCAGCTGTGTAGTCTGGCCCCTGCTGACTATTCTGCCTGT
GCCCGCTCAGGCCTCACCCTCAACCGATACAGCACATCTTTGGCAGAGACCTACAACAGGCTCACCAACC
AGGCTGGTGAGACCTTTGCCCCGCCCAGGACTCCCTCCTATGTGGGCGTGAGCAGCAGCACCTCCGTGAA
CATGTCCATGGGTGGCACTGATGGGGACACCTTCAGCTGCCCACAGACCAGCTTATCCATGCAGATTTCG
GGAATGTCCCCCCAGCTCCAGTATATCATGCCATCACCCTCCAGCAATGCCTTCGCCACTAACCAGACCC
ATCAGGGTTCCTATAATACTTTTAGATTACACAGCCCCTGTGCACTATATGGATATAACTTCTCCACATC
CCCCAAACTGGCTGCCAGTCCTGAGAAAATTGTTTCTTCCCAAGGAAGTTTCTTGGGGTCCTCACCGAGT
GGGACCATGACGGATCGGCAGATGTTGCCCCCTGTGGAAGGAGTGCACCTGCTTAGCAGTGGGGGTCAGC
AGAGTTTCTTTGACTCTAGGACCCTAGGAAGCTTAACTCTGTCATCATCTCAAGTATCTGCACATATGGT
C


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211838 representing NM_001080508
Red=Cloning site Green=Tags(s)

MAEKRRGSPCSMLSLKAHAFSVEALIGAEKQQQLQKKRRKLGAEEAARAVDDGGCSRGGGAGEKGSSEGD
EGAALPPPAGATSGPARSGADLERGAAGGCEDGFQQGASPLASPGGSPKGSPARSLARPGTPLPSPQAPR
VDLQGAELWKRFHEIGTEMIITKAGRRMFPAMRVKISGLDPHQQYYIAMDIVPVDNKRYRYVYHSSKWMV
AGNADSPVPPRVYIHPDSPASGETWMRQVISFDKLKLTNNELDDQGHIILHSMHKYQPRVHVIRKDCGDD
LSPIKPVPSGEGVKAFSFPETVFTTVTAYQNQQITRLKIDRNPFAKGFRDSGRNRMGLEALVESYAFWRP
SLRTLTFEDIPGIPKQGNASSSTLLQGTGNGVPATHPHLLSGSSCSSPAFHLGPNTSQLCSLAPADYSAC
ARSGLTLNRYSTSLAETYNRLTNQAGETFAPPRTPSYVGVSSSTSVNMSMGGTDGDTFSCPQTSLSMQIS
GMSPQLQYIMPSPSSNAFATNQTHQGSYNTFRLHSPCALYGYNFSTSPKLAASPEKIVSSQGSFLGSSPS
GTMTDRQMLPPVEGVHLLSSGGQQSFFDSRTLGSLTLSSSQVSAHMV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001080508
ORF Size 1821 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001080508.3
RefSeq Size 4070 bp
RefSeq ORF 1824 bp
Locus ID 9096
UniProt ID O95935
Cytogenetics 6q14.3
MW 64.6 kDa
Gene Summary This genes codes for a member of an evolutionarily conserved family of transcription factors that plays a crucial role in embryonic development. The family is characterized by the presence of the DNA-binding T-box domain and is divided into five sub-families based on sequence conservation in this domain. The encoded protein belongs to the vertebrate specific Tbx1 sub-family. The protein acts as a transcriptional repressor by antagonizing transcriptional activators in the T-box family. The protein forms homo- or heterodimers with other transcription factors of the T-box family or other transcription factors. [provided by RefSeq, Nov 2012]
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