PDGF Receptor alpha (PDGFRA) (NM_006206) Human Tagged ORF Clone
Lenti ORF clone of Human platelet-derived growth factor receptor, alpha polypeptide (PDGFRA), mGFP tagged
|Product Name||PDGF Receptor alpha (PDGFRA) (NM_006206) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||CD140A; PDGFR-2; PDGFR2|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||3267 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||6405 bp|
|RefSeq ORF||3270 bp|
|Domains||pkinase, TyrKc, S_TKc, ig, IGc2, IG|
|Protein Families||Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane|
|Protein Pathways||Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Endocytosis, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton|
|Gene Summary||This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers. [provided by RefSeq, Mar 2012]|
|cDNA Clone Resources|
|SC116256||PDGFRA (untagged)-Human platelet-derived growth factor receptor, alpha polypeptide (PDGFRA)||
|SC323694||PDGFRA (untagged)-Kinase deficient mutant (K627M) of Human platelet-derived growth factor receptor, alpha polypeptide (PDGFRA)||
|RC211740||PDGFRA (Myc-DDK-tagged)-Human platelet-derived growth factor receptor, alpha polypeptide (PDGFRA)||
|RC600016||PDGFRA (DDK-His-tagged)-Extra Cellular Domain Clone of Homo sapiens platelet-derived growth factor receptor, alpha polypeptide, Signal peptide (1-23) plus EC domain (24-528)||
|RG211740||PDGFRA (GFP-tagged) - Human platelet-derived growth factor receptor, alpha polypeptide (PDGFRA)||
|RC211740L1||Lenti ORF clone of Human platelet-derived growth factor receptor, alpha polypeptide (PDGFRA), Myc-DDK-tagged||
|RC211740L3||Lenti ORF clone of Human platelet-derived growth factor receptor, alpha polypeptide (PDGFRA), Myc-DDK-tagged||
|RC211740L4||Lenti ORF clone of Human platelet-derived growth factor receptor, alpha polypeptide (PDGFRA), mGFP tagged||