ORC4L (ORC4) (NM_181741) Human Tagged ORF Clone

CAT#: RC211723

ORC4 (Myc-DDK-tagged)-Human origin recognition complex, subunit 4 (ORC4), transcript variant 1

Product Images

Circular map for RC211723

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  Western blot validation of overexpression lysate (Cat# LY405645) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211723 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ORC4 protein (Cat# TP311723). The protein was produced from HEK293T cells transfected with ORC4 cDNA clone (Cat# RC211723) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ORC4L
• Synonyms: ORC4L; ORC4P
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211723 representing NM_181741
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGTCGTAAATCAAAGAGTAACAGCTTAATTCACACAGAGTGCCTTTCACAGGTACAAAGAATTT
  TACGTGAAAGATTTTGTCGTCAGAGTCCACATAGTAACCTATTTGGAGTGCAAGTACAATACAAACACTT
  AAGTGAGCTGCTGAAAAGAACTGCTCTCCATGGAGAGAGTAACTCTGTCCTTATTATCGGACCCCGAGGA
  TCAGGAAAAACTATGTTAATAAATCATGCTTTGAAAGAACTCATGGAAATAGAAGAAGTGAGTGAAAATG
  TATTACAAGTTCACTTAAATGGACTGCTGCAGATCAATGACAAAATCGCCCTAAAGGAAATCACAAGGCA
  GTTAAATCTGGAAAATGTAGTTGGAGATAAAGTTTTTGGAAGCTTTGCTGAAAACCTTTCATTTCTTCTG
  GAAGCTTTAAAAAAAGGTGACCGAACTAGCAGTTGCCCAGTGATCTTCATATTAGATGAATTTGATCTTT
  TTGCTCATCATAAAAACCAAACACTTCTCTATAATCTTTTTGACATTTCTCAGTCTGCACAGACCCCAAT
  AGCAGTTATTGGTCTTACATGTAGATTGGATATTTTGGAACTCTTAGAAAAAAGAGTGAAGTCAAGATTT
  TCTCACCGGCAGATACACTTAATGAATTCATTTGGTTTTCCACAGTATGTTAAAATATTTAAAGAACAGT
  TATCTCTACCTGCAGAGTTTCCAGACAAGGTTTTTGCTGAGAAGTGGAATGAAAATGTTCAGTATCTCTC
  AGAAGATAGAAGTGTGCAAGAAGTACTACAGAAGCATTTCAATATCAGCAAAAACCTGCGGTCATTACAC
  ATGCTATTGATGCTTGCTTTAAATCGAGTAACAGCATCGCACCCATTTATGACTGCCGTAGATCTAATGG
  AAGCAAGCCAACTGTGTAGCATGGACTCGAAAGCAAATATTGTACATGGTCTATCAGTCTTGGAAATCTG
  TCTTATAATAGCAATGAAACATTTAAATGACATCTATGAGGAAGAGCCATTTAATTTTCAAATGGTCTAT
  AATGAGTTTCAGAAGTTTGTTCAAAGGAAAGCACATTCCGTTTATAATTTTGAAAAACCTGTTGTCATGA
  AGGCTTTTGAACACTTGCAGCAATTAGAATTAATAAAGCCCATGGAAAGAACTTCAGGAAATTCACAGAG
  AGAGTACCAGCTGATGAAACTGCTTTTGGATAATACTCAAATTATGAATGCTCTGCAGAAATATCCCAAC
  TGTCCTACAGATGTGAGGCAGTGGGCAACATCCTCACTAAGCTGGTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211723 representing NM_181741
  Red=Cloning site Green=Tags(s)
  
  MSSRKSKSNSLIHTECLSQVQRILRERFCRQSPHSNLFGVQVQYKHLSELLKRTALHGESNSVLIIGPRG
  SGKTMLINHALKELMEIEEVSENVLQVHLNGLLQINDKIALKEITRQLNLENVVGDKVFGSFAENLSFLL
  EALKKGDRTSSCPVIFILDEFDLFAHHKNQTLLYNLFDISQSAQTPIAVIGLTCRLDILELLEKRVKSRF
  SHRQIHLMNSFGFPQYVKIFKEQLSLPAEFPDKVFAEKWNENVQYLSEDRSVQEVLQKHFNISKNLRSLH
  MLLMLALNRVTASHPFMTAVDLMEASQLCSMDSKANIVHGLSVLEICLIIAMKHLNDIYEEEPFNFQMVY
  NEFQKFVQRKAHSVYNFEKPVVMKAFEHLQQLELIKPMERTSGNSQREYQLMKLLLDNTQIMNALQKYPN
  CPTDVRQWATSSLSWL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181741
• ORF Size: 1308 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181741.4
• RefSeq Size: 2793 bp
• RefSeq ORF: 1311 bp
• Locus ID: 5000
• UniProt ID: O43929
• Cytogenetics: 2q23.1
• Protein Pathways: Cell cycle
• MW: 50.2 kDa
• Gene Summary: The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. This gene encodes a subunit of the ORC complex. Several alternatively spliced transcript variants, some of which encode the same protein, have been reported for this gene. [provided by RefSeq, Oct 2010]