L1CAM (NM_000425) Human Tagged ORF Clone

CAT#: RC211601

L1CAM (Myc-DDK-tagged)-Human L1 cell adhesion molecule (L1CAM), transcript variant 1

Product Images

Circular map for RC211601

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY L1CAM (Cat# RC211601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM (Cat# TA500728). Positive lysates LY400150 (100ug) and LC400150 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400150) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211601 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified L1CAM protein (Cat# TP311601). The protein was produced from HEK293T cells transfected with L1CAM cDNA clone (Cat# RC211601) using MegaTran 2.0 (Cat# TT210002).

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  Anti-L1CAM mouse monoclonal antibody (TA500728) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM (RC211601).

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Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L1CAM
• Synonyms: CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211601 representing NM_000425
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCGTGGCGCTGCGGTACGTGTGGCCTCTCCTCCTCTGCAGCCCCTGCCTGCTTATCCAGATCCCCG
  AGGAATATGAAGGACACCATGTGATGGAGCCACCTGTCATCACGGAACAGTCTCCACGGCGCCTGGTTGT
  CTTCCCCACAGATGACATCAGCCTCAAGTGTGAGGCCAGTGGCAAGCCCGAAGTGCAGTTCCGCTGGACG
  AGGGATGGTGTCCACTTCAAACCCAAGGAAGAGCTGGGTGTGACCGTGTACCAGTCGCCCCACTCTGGCT
  CCTTCACCATCACGGGCAACAACAGCAACTTTGCTCAGAGGTTCCAGGGCATCTACCGCTGCTTTGCCAG
  CAATAAGCTGGGCACCGCCATGTCCCATGAGATCCGGCTCATGGCCGAGGGTGCCCCCAAGTGGCCAAAG
  GAGACAGTGAAGCCCGTGGAGGTGGAGGAAGGGGAGTCAGTGGTTCTGCCTTGCAACCCTCCCCCAAGTG
  CAGAGCCTCTCCGGATCTACTGGATGAACAGCAAGATCTTGCACATCAAGCAGGACGAGCGGGTGACGAT
  GGGCCAGAACGGCAACCTCTACTTTGCCAATGTGCTCACCTCCGACAACCACTCAGACTACATCTGCCAC
  GCCCACTTCCCAGGCACCAGGACCATCATTCAGAAGGAACCCATTGACCTCCGGGTCAAGGCCACCAACA
  GCATGATTGACAGGAAGCCGCGCCTGCTCTTCCCCACCAACTCCAGCAGCCACCTGGTGGCCTTGCAGGG
  GCAGCCATTGGTCCTGGAGTGCATCGCCGAGGGCTTTCCCACGCCCACCATCAAATGGCTGCGCCCCAGT
  GGCCCCATGCCAGCCGACCGTGTCACCTACCAGAACCACAACAAGACCCTGCAGCTGCTGAAAGTGGGCG
  AGGAGGATGATGGCGAGTACCGCTGCCTGGCCGAGAACTCACTGGGCAGTGCCCGGCATGCGTACTATGT
  CACCGTGGAGGCTGCCCCGTACTGGCTGCACAAGCCCCAGAGCCATCTATATGGGCCAGGAGAGACTGCC
  CGCCTGGACTGCCAAGTCCAGGGCAGGCCCCAACCAGAGGTCACCTGGAGAATCAACGGGATCCCTGTGG
  AGGAGCTGGCCAAAGACCAGAAGTACCGGATTCAGCGTGGCGCCCTGATCCTGAGCAACGTGCAGCCCAG
  TGACACAATGGTGACCCAATGTGAGGCCCGCAACCGGCACGGGCTCTTGCTGGCCAATGCCTACATCTAC
  GTTGTCCAGCTGCCAGCCAAGATCCTGACTGCGGACAATCAGACGTACATGGCTGTCCAGGGCAGCACTG
  CCTACCTTCTGTGCAAGGCCTTCGGAGCGCCTGTGCCCAGTGTTCAGTGGCTGGACGAGGATGGGACAAC
  AGTGCTTCAGGACGAACGCTTCTTCCCCTATGCCAATGGGACCCTGGGCATTCGAGACCTCCAGGCCAAT
  GACACCGGACGCTACTTCTGCCTGGCTGCCAATGACCAAAACAATGTTACCATCATGGCTAACCTGAAGG
  TTAAAGATGCAACTCAGATCACTCAGGGGCCCCGCAGCACAATCGAGAAGAAAGGTTCCAGGGTGACCTT
  CACGTGCCAGGCCTCCTTTGACCCCTCCTTGCAGCCCAGCATCACCTGGCGTGGGGACGGTCGAGACCTC
  CAGGAGCTTGGGGACAGTGACAAGTACTTCATAGAGGATGGGCGCCTGGTCATCCACAGCCTGGACTACA
  GCGACCAGGGCAACTACAGCTGCGTGGCCAGTACCGAACTGGATGTGGTGGAGAGTAGGGCACAGCTCTT
  GGTGGTGGGGAGCCCTGGGCCGGTGCCACGGCTGGTGCTGTCCGACCTGCACCTGCTGACGCAGAGCCAG
  GTGCGCGTGTCCTGGAGTCCTGCAGAAGACCACAATGCCCCCATTGAGAAATATGACATTGAATTTGAGG
  ACAAGGAAATGGCGCCTGAAAAATGGTACAGTCTGGGCAAGGTTCCAGGGAACCAGACCTCTACCACCCT
  CAAGCTGTCGCCCTATGTCCACTACACCTTTAGGGTTACTGCCATAAACAAATATGGCCCCGGGGAGCCC
  AGCCCGGTCTCTGAGACTGTGGTCACACCTGAGGCAGCCCCAGAGAAGAACCCTGTGGATGTGAAGGGGG
  AAGGAAATGAGACCACCAATATGGTCATCACGTGGAAGCCGCTCCGGTGGATGGACTGGAACGCCCCCCA
  GGTTCAGTACCGCGTGCAGTGGCGCCCTCAGGGGACACGAGGGCCCTGGCAGGAGCAGATTGTCAGCGAC
  CCCTTCCTGGTGGTGTCCAACACGTCCACCTTCGTGCCCTATGAGATCAAAGTCCAGGCCGTCAACAGCC
  AGGGCAAGGGACCAGAGCCCCAGGTCACTATCGGCTACTCTGGAGAGGACTACCCCCAGGCAATCCCTGA
  GCTGGAAGGCATTGAAATCCTCAACTCAAGTGCCGTGCTGGTCAAGTGGCGGCCGGTGGACCTGGCCCAG
  GTCAAGGGCCACCTCCGCGGATACAATGTGACGTACTGGAGGGAGGGCAGTCAGAGGAAGCACAGCAAGA
  GACATATCCACAAAGACCATGTGGTGGTGCCCGCCAACACCACCAGTGTCATCCTCAGTGGCTTGCGGCC
  CTATAGCTCCTACCACCTGGAGGTGCAGGCCTTTAACGGGCGAGGATCGGGGCCCGCCAGCGAGTTCACC
  TTCAGCACCCCAGAGGGAGTGCCTGGCCACCCCGAGGCGTTGCACCTGGAGTGCCAGTCGAACACCAGCC
  TGCTGCTGCGCTGGCAGCCCCCACTCAGCCACAACGGCGTGCTCACCGGCTACGTGCTCTCCTACCACCC
  CCTGGATGAGGGGGGCAAGGGGCAACTGTCCTTCAACCTTCGGGACCCCGAACTTCGGACACACAACCTG
  ACCGATCTCAGCCCCCACCTGCGGTACCGCTTCCAGCTTCAGGCCACCACCAAAGAGGGCCCTGGTGAAG
  CCATCGTACGGGAAGGAGGCACTATGGCCTTGTCTGGGATCTCAGATTTTGGCAACATCTCAGCCACAGC
  GGGTGAAAACTACAGTGTCGTCTCCTGGGTCCCCAAGGAGGGCCAGTGCAACTTCAGGTTCCATATCTTG
  TTCAAAGCCTTGGGAGAAGAGAAGGGTGGGGCTTCCCTTTCGCCACAGTATGTCAGCTACAACCAGAGCT
  CCTACACGCAGTGGGACCTGCAGCCTGACACTGACTACGAGATCCACTTGTTTAAGGAGAGGATGTTCCG
  GCACCAAATGGCTGTGAAGACCAATGGCACAGGCCGCGTGAGGCTCCCTCCTGCTGGCTTCGCCACTGAG
  GGCTGGTTCATCGGCTTTGTGAGTGCCATCATCCTCCTGCTCCTCGTCCTGCTCATCCTCTGCTTCATCA
  AGCGCAGCAAGGGCGGCAAATACTCAGTGAAGGATAAGGAGGACACCCAGGTGGACTCTGAGGCCCGACC
  GATGAAAGATGAGACCTTCGGCGAGTACAGGTCCCTGGAGAGTGACAACGAGGAGAAGGCCTTTGGCAGC
  AGCCAGCCATCGCTCAACGGGGACATCAAGCCCCTGGGCAGTGACGACAGCCTGGCCGATTATGGGGGCA
  GCGTGGATGTTCAGTTCAACGAGGATGGTTCGTTCATTGGCCAGTACAGTGGCAAGAAGGAGAAGGAGGC
  GGCAGGGGGCAATGACAGCTCAGGGGCCACTTCCCCCATCAACCCTGCCGTGGCCCTAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211601 representing NM_000425
  Red=Cloning site Green=Tags(s)
  
  MVVALRYVWPLLLCSPCLLIQIPEEYEGHHVMEPPVITEQSPRRLVVFPTDDISLKCEASGKPEVQFRWT
  RDGVHFKPKEELGVTVYQSPHSGSFTITGNNSNFAQRFQGIYRCFASNKLGTAMSHEIRLMAEGAPKWPK
  ETVKPVEVEEGESVVLPCNPPPSAEPLRIYWMNSKILHIKQDERVTMGQNGNLYFANVLTSDNHSDYICH
  AHFPGTRTIIQKEPIDLRVKATNSMIDRKPRLLFPTNSSSHLVALQGQPLVLECIAEGFPTPTIKWLRPS
  GPMPADRVTYQNHNKTLQLLKVGEEDDGEYRCLAENSLGSARHAYYVTVEAAPYWLHKPQSHLYGPGETA
  RLDCQVQGRPQPEVTWRINGIPVEELAKDQKYRIQRGALILSNVQPSDTMVTQCEARNRHGLLLANAYIY
  VVQLPAKILTADNQTYMAVQGSTAYLLCKAFGAPVPSVQWLDEDGTTVLQDERFFPYANGTLGIRDLQAN
  DTGRYFCLAANDQNNVTIMANLKVKDATQITQGPRSTIEKKGSRVTFTCQASFDPSLQPSITWRGDGRDL
  QELGDSDKYFIEDGRLVIHSLDYSDQGNYSCVASTELDVVESRAQLLVVGSPGPVPRLVLSDLHLLTQSQ
  VRVSWSPAEDHNAPIEKYDIEFEDKEMAPEKWYSLGKVPGNQTSTTLKLSPYVHYTFRVTAINKYGPGEP
  SPVSETVVTPEAAPEKNPVDVKGEGNETTNMVITWKPLRWMDWNAPQVQYRVQWRPQGTRGPWQEQIVSD
  PFLVVSNTSTFVPYEIKVQAVNSQGKGPEPQVTIGYSGEDYPQAIPELEGIEILNSSAVLVKWRPVDLAQ
  VKGHLRGYNVTYWREGSQRKHSKRHIHKDHVVVPANTTSVILSGLRPYSSYHLEVQAFNGRGSGPASEFT
  FSTPEGVPGHPEALHLECQSNTSLLLRWQPPLSHNGVLTGYVLSYHPLDEGGKGQLSFNLRDPELRTHNL
  TDLSPHLRYRFQLQATTKEGPGEAIVREGGTMALSGISDFGNISATAGENYSVVSWVPKEGQCNFRFHIL
  FKALGEEKGGASLSPQYVSYNQSSYTQWDLQPDTDYEIHLFKERMFRHQMAVKTNGTGRVRLPPAGFATE
  GWFIGFVSAIILLLLVLLILCFIKRSKGGKYSVKDKEDTQVDSEARPMKDETFGEYRSLESDNEEKAFGS
  SQPSLNGDIKPLGSDDSLADYGGSVDVQFNEDGSFIGQYSGKKEKEAAGGNDSSGATSPINPAVALE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000425
• ORF Size: 3771 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_000425.5
• RefSeq Size: 4525 bp
• RefSeq ORF: 3774 bp
• Locus ID: 3897
• UniProt ID: P32004
• Cytogenetics: Xq28
• Domains: ig, IGc2, IG, FN3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Axon guidance, Cell adhesion molecules (CAMs)
• MW: 140 kDa
• Gene Summary: The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]