CACNA1F (NM_005183) Human Tagged ORF Clone

CAT#: RC211587

CACNA1F (Myc-DDK-tagged)-Human calcium channel, voltage-dependent, L type, alpha 1F subunit (CACNA1F)

Product Images

Circular map for RC211587

• 

  Western blot validation of overexpression lysate (Cat# LY417463) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211587 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CACNA1F
• Synonyms: AIED; Cav1.4; Cav1.4alpha1; COD3; COD4; CORDX; CORDX3; CSNB2; CSNB2A; CSNBX2; JM8; JMC8; OA2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211587 representing NM_005183
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGAATCTGAAGGCGGGAAAGACACCACCCCAGAGCCCAGTCCAGCCAATGGGGCAGGCCCTGGTC
  CCGAATGGGGGCTGTGCCCCGGGCCCCCAGCTGTGGAAGGTGAAAGCAGTGGGGCATCAGGCCTAGGGAC
  CCCTAAGCGAAGAAACCAGCACAGCAAGCACAAGACAGTGGCAGTGGCCAGTGCCCAGCGGTCACCTCGG
  GCACTCTTCTGCCTCACCCTGGCCAATCCTCTGCGACGGTCCTGCATCAGCATCGTGGAGTGGAAGCCCT
  TCGACATCCTCATCCTGCTGACCATCTTTGCCAACTGCGTGGCCCTGGGAGTTTACATCCCCTTCCCTGA
  GGACGACTCCAACACTGCCAACCACAACCTGGAGCAGGTGGAGTACGTATTCCTGGTGATTTTCACTGTG
  GAGACGGTGCTCAAGATCGTGGCCTACGGGCTGGTGCTCCACCCCAGCGCCTACATCCGCAATGGCTGGA
  ACCTACTCGACTTCATCATCGTCGTGGTCGGGCTGTTCAGCGTTCTGCTGGAGCAGGGCCCCGGACGGCC
  AGGCGACGCCCCGCACACCGGGGGAAAGCCAGGAGGCTTCGATGTGAAGGCATTGAGGGCGTTTCGGGTG
  CTGCGGCCACTGAGGCTGGTGTCTGGGGTCCCGAGCCTGCACATAGTGCTCAATTCCATCATGAAGGCTC
  TGGTGCCGCTGCTGCACATTGCACTGCTCGTGCTCTTCGTCATCATCATTTATGCCATCATTGGGCTCGA
  GCTGTTCCTTGGACGAATGCACAAGACGTGCTACTTCCTGGGATCCGACATGGAAGCGGAGGAGGACCCA
  TCGCCCTGTGCGTCTTCGGGATCAGGGCGTGCGTGCACGCTGAACCAGACTGAGTGCCGCGGGCGCTGGC
  CAGGGCCCAATGGAGGCATCACCAACTTTGACAACTTCTTCTTCGCCATGCTGACAGTCTTCCAGTGTGT
  CACCATGGAAGGCTGGACCGATGTGCTCTACTGGATGCAAGATGCCATGGGGTATGAACTGCCCTGGGTG
  TACTTTGTGAGCCTTGTCATCTTTGGGTCCTTCTTCGTCCTCAACCTTGTGCTTGGCGTCCTGAGTGGGG
  AGTTCTCCAAGGAGAGAGAGAAAGCGAAAGCTCGCGGGGACTTCCAGAAGCAGCGGGAGAAGCAGCAGAT
  GGAGGAAGACCTGCGGGGCTACCTGGACTGGATCACTCAAGCCGAAGAGCTGGACATGGAGGACCCCTCC
  GCCGATGACAACCTTGGTTCTATGGCTGAAGAGGGCCGGGCGGGCCATCGGCCACAGCTGGCCGAGCTGA
  CCAATAGGAGGCGTGGACGTCTGCGCTGGTTCAGTCATTCTACTCGCTCCACACACTCCACCAGCAGCCA
  TGCCAGCCTCCCAGCCAGTGACACCGGTTCCATGACAGAGACCCAAGGCGATGAGGATGAGGAGGAGGGG
  GCTCTGGCCAGCTGTACACGCTGCCTAAACAAGATCATGAAAACCAGAGTCTGCCGCCGCCTCCGCCGAG
  CCAACCGGGTCCTTCGGGCACGCTGCCGTCGGGCAGTGAAGTCCAATGCCTGCTACTGGGCTGTGCTGTT
  GCTCGTCTTCCTCAACACGTTGACCATCGCCTCTGAGCACCACGGGCAGCCTGTGTGGCTCACCCAGATC
  CAGGAGTATGCCAACAAAGTGTTGCTCTGTCTGTTCACGGTGGAGATGCTTCTCAAATTGTACGGTCTGG
  GCCCCTCTGCCTATGTGTCTTCCTTCTTCAACCGCTTTGACTGCTTTGTGGTCTGTGGGGGCATCCTAGA
  GACCACCTTGGTGGAGGTGGGCGCCATGCAGCCCTTGGGCATCTCAGTGCTCCGATGTGTGCGCCTCCTC
  AGGATCTTTAAGGTCACCAGACACTGGGCTTCTCTGAGCAATCTGGTGGCATCCCTGCTCAATTCAATGA
  AATCCATCGCATCCTTGCTGCTTCTCCTCTTCCTCTTCATCATTATCTTCTCCCTGCTTGGCATGCAGCT
  GTTTGGGGGCAAGTTCAACTTTGACCAGACCCACACCAAGCGAAGCACCTTTGACACGTTCCCCCAGGCC
  CTCCTCACTGTCTTTCAGATCCTGACAGGTGAGGACTGGAACGTGGTCATGTATGATGGTATCATGGCAT
  ATGGTGGCCCCTTCTTCCCAGGAATGTTGGTGTGCATCTATTTCATCATTCTCTTCATCTGTGGCAACTA
  CATCCTGTTGAACGTGTTTCTTGCCATTGCTGTGGACAACCTGGCCAGTGGAGATGCAGGCACTGCCAAG
  GACAAGGGCGGGGAGAAGAGCAATGAGAAGGATCTCCCACAGGAGAATGAAGGCCTGGTGCCTGGTGTGG
  AGAAAGAGGAAGAGGAGGGTGCAAGGAGGGAAGGAGCAGACATGGAGGAGGAGGAGGAGGAGGAAGAAGA
  GGAAGAAGAGGAAGAAGAGGAAGAGGGTGCAGGGGGTGTGGAACTCCTGCAGGAAGTTGTACCCAAGGAG
  AAGGTGGTACCCATCCCTGAGGGCAGCGCCTTCTTCTGCCTCAGCCAAACCAACCCGCTGAGGAAGGGCT
  GCCACACCCTCATCCACCATCATGTCTTCACCAATCTTATCCTGGTGTTCATCATCCTCAGCAGTGTGTC
  CCTGGCCGCTGAGGACCCCATCCGAGCCCACTCCTTCCGCAACCATATTCTGGGTTACTTCGATTATGCC
  TTCACCTCCATTTTCACTGTGGAGATTCTACTAAAGATGACAGTGTTTGGGGCCTTCCTGCACCGCGGCT
  CCTTCTGCCGTAGCTGGTTTAATATGTTGGATCTGCTGGTGGTCAGTGTGTCCCTCATCTCCTTTGGCAT
  CCACTCCAGCGCCATCTCGGTGGTGAAGATTCTGCGAGTACTCCGAGTACTGCGGCCCCTCCGAGCCATC
  AACAGGGCCAAGGGACTCAAGCATGTGGTGCAGTGTGTATTTGTGGCCATCCGGACCATCGGAAACATCA
  TGATTGTCACCACACTTCTGCAATTTATGTTCGCCTGCATCGGGGTGCAGCTCTTCAAGGGGAAATTCTA
  CACCTGCACGGACGAGGCCAAACACACCCCTCAAGAATGCAAGGGCTCCTTCCTGGTATACCCAGATGGA
  GACGTGTCACGGCCCCTGGTCCGGGAGCGGCTCTGGGTCAACAGTGATTTCAACTTTGACAATGTCCTTT
  CAGCCATGATGGCCCTGTTCACTGTCTCCACCTTTGAAGGCTGGCCTGCACTGCTATACAAGGCCATCGA
  TGCATATGCAGAGGACCATGGCCCCATCTATAATTACCGTGTGGAGATCTCAGTGTTCTTCATTGTCTAC
  ATCATCATCATTGCGTTCTTCATGATGAACATCTTCGTGGGCTTCGTCATCATCACTTTCCGTGCCCAGG
  GCGAGCAGGAGTACCAAAACTGTGAGCTGGACAAGAACCAGCGTCAATGTGTGGAATATGCCCTCAAGGC
  CCAGCCACTCCGCCGTTACATCCCCAAGAACCCGCATCAGTATCGTGTGTGGGCCACTGTGAACTCTGCT
  GCCTTTGAGTACCTGATGTTCCTGCTCATCCTGCTCAACACAGTTGCCCTAGCCATGCAGCACTATGAGC
  AGACTGCTCCCTTCAACTATGCCATGGACATCCTCAACATGGTCTTCACTGGCCTCTTCACTATTGAGAT
  GGTGCTCAAAATCATCGCCTTCAAGCCCAAGCATTACTTCACTGATGCCTGGAACACGTTTGACGCTCTT
  ATTGTGGTGGGCAGCATAGTGGATATTGCCGTCACTGAAGTCAATAATGGTGGCCACCTTGGCGAGAGCT
  CTGAGGACAGCTCCCGCATTTCCATTACCTTCTTTCGCCTCTTCCGAGTTATGCGGCTGGTCAAGCTTCT
  CAGTAAGGGTGAAGGGATCCGCACATTGCTCTGGACATTCATCAAGTCCTTCCAGGCCTTGCCCTATGTG
  GCTCTTCTCATCGCAATGATATTCTTCATCTATGCCGTCATTGGCATGCAGATGTTCGGCAAGGTGGCTC
  TTCAGGATGGCACACAGATAAACCGAAACAACAACTTCCAGACCTTTCCACAGGCTGTGCTGCTTCTGTT
  CAGGTGTGCCACTGGTGAGGCATGGCAGGAGATAATGCTTGCCAGCCTTCCCGGAAATCGGTGTGATCCT
  GAGTCTGACTTCGGCCCTGGTGAAGAGTTTACCTGTGGTAGCAATTTTGCCATCGCCTATTTCATCAGCT
  TCTTCATGCTCTGTGCCTTCCTGATCATAAATCTCTTTGTGGCTGTGATCATGGACAACTTTGATTATCT
  CACCAGAGATTGGTCCATCCTGGGCCCCCATCACCTTGATGAATTCAAGAGGATCTGGTCTGAATATGAC
  CCTGGGGCCAAGGGCCGCATCAAACACTTGGATGTGGTTGCCCTGCTGAGACGTATCCAGCCCCCTCTGG
  GATTTGGGAAGCTGTGCCCACACCGAGTGGCCTGCAAGAGACTTGTGGCAATGAACATGCCCCTCAACTC
  AGATGGGACGGTGACATTCAACGCCACACTCTTTGCCCTGGTCCGGACATCCCTGAAGATCAAAACAGAA
  GGGAACCTGGAGCAAGCCAACCAGGAGCTGCGGATTGTCATCAAAAAGATCTGGAAGCGGATGAAACAGA
  AGCTGCTAGATGAGGTCATCCCCCCACCAGACGAGGAGGAGGTCACCGTGGGCAAATTCTACGCCACATT
  TCTGATCCAGGACTATTTCCGCAAATTCCGGCGGAGGAAAGAAAAAGGGCTACTAGGCAACGACGCCGCC
  CCTAGCACCTCTTCCGCCCTTCAGGCTGGTCTGCGGAGCCTGCAGGACTTGGGTCCTGAGATGCGGCAGG
  CCCTCACCTGTGACACAGAGGAGGAGGAAGAAGAGGGGCAGGAGGGAGTGGAGGAGGAAGATGAAAAGGA
  CTTGGAAACTAACAAAGCCACGATGGTCTCCCAGCCCTCAGCTCGCCGGGGCTCCGGGATTTCTGTGTCT
  CTGCCTGTCGGGGACAGACTTCCAGATTCACTCTCCTTTGGGCCCAGTGATGATGACAGGGGGACTCCCA
  CCTCCAGTCAGCCCAGTGTGCCCCAGGCTGGATCCAACACCCACAGGAGAGGCTCTGGGGCTCTCATTTT
  CACCATCCCAGAAGAAGGAAATTCTCAGCCCAAGGGAACCAAAGGGCAAAACAAGCAAGATGAGGATGAG
  GAAGTCCCTGATCGGCTTTCCTACCTAGATGAGCAGGCAGGGACTCCCCCGTGCTCAGTCCTTTTGCCAC
  CTCACAGAGCTCAGAGATACATGGATGGGCACCTGGTACCACGCCGCCGTCTGCTGCCCCCCACACCTGC
  AGGTCGGAAGCCCTCCTTCACCATCCAGTGTCTGCAGCGCCAGGGCAGTTGTGAGGATTTACCCATCCCA
  GGCACCTATCATCGTGGGCGAAATTCAGGGCCCAATAGGGCTCAGGGTTCCTGGGCAACACCACCTCAGC
  GGGGTCGGCTCCTGTATGCCCCGCTGTTGTTGGTGGAAGAGGGCGCAGCGGGGGAGGGGTACCTCGGCAG
  ATCCAGTGGCCCACTGCGCACCTTCACCTGTCTGCACGTGCCTGGAACCCACTCGGACCCCAGCCATGGG
  AAGAGGGGCAGTGCCGACAGCTTGGTGGAGGCTGTGCTTATCTCAGAGGGTCTGGGCCTCTTTGCTCGAG
  ACCCACGTTTCGTGGCCCTGGCCAAGCAGGAGATTGCAGATGCGTGTCGCCTGACGCTGGATGAGATGGA
  CAATGCTGCCAGTGACCTGCTGGCACAGGGAACCAGCTCTCTCTATAGCGACGAGGAGTCCATCCTCTCC
  CGCTTCGATGAGGAGGACTTGGGAGACGAGATGGCCTGCGTCCACGCCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211587 representing NM_005183
  Red=Cloning site Green=Tags(s)
  
  MSESEGGKDTTPEPSPANGAGPGPEWGLCPGPPAVEGESSGASGLGTPKRRNQHSKHKTVAVASAQRSPR
  ALFCLTLANPLRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTV
  ETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRV
  LRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDMEAEEDP
  SPCASSGSGRACTLNQTECRGRWPGPNGGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMGYELPWV
  YFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKQREKQQMEEDLRGYLDWITQAEELDMEDPS
  ADDNLGSMAEEGRAGHRPQLAELTNRRRGRLRWFSHSTRSTHSTSSHASLPASDTGSMTETQGDEDEEEG
  ALASCTRCLNKIMKTRVCRRLRRANRVLRARCRRAVKSNACYWAVLLLVFLNTLTIASEHHGQPVWLTQI
  QEYANKVLLCLFTVEMLLKLYGLGPSAYVSSFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLL
  RIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQA
  LLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLASGDAGTAK
  DKGGEKSNEKDLPQENEGLVPGVEKEEEEGARREGADMEEEEEEEEEEEEEEEEEGAGGVELLQEVVPKE
  KVVPIPEGSAFFCLSQTNPLRKGCHTLIHHHVFTNLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYA
  FTSIFTVEILLKMTVFGAFLHRGSFCRSWFNMLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAI
  NRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYTCTDEAKHTPQECKGSFLVYPDG
  DVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAYAEDHGPIYNYRVEISVFFIVY
  IIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSA
  AFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTIEMVLKIIAFKPKHYFTDAWNTFDAL
  IVVGSIVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYV
  ALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGNRCDP
  ESDFGPGEEFTCGSNFAIAYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYD
  PGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSLKIKTE
  GNLEQANQELRIVIKKIWKRMKQKLLDEVIPPPDEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGNDAA
  PSTSSALQAGLRSLQDLGPEMRQALTCDTEEEEEEGQEGVEEEDEKDLETNKATMVSQPSARRGSGISVS
  LPVGDRLPDSLSFGPSDDDRGTPTSSQPSVPQAGSNTHRRGSGALIFTIPEEGNSQPKGTKGQNKQDEDE
  EVPDRLSYLDEQAGTPPCSVLLPPHRAQRYMDGHLVPRRRLLPPTPAGRKPSFTIQCLQRQGSCEDLPIP
  GTYHRGRNSGPNRAQGSWATPPQRGRLLYAPLLLVEEGAAGEGYLGRSSGPLRTFTCLHVPGTHSDPSHG
  KRGSADSLVEAVLISEGLGLFARDPRFVALAKQEIADACRLTLDEMDNAASDLLAQGTSSLYSDEESILS
  RFDEEDLGDEMACVHAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005183
• ORF Size: 5931 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005183.2, NP_005174.2
• RefSeq Size: 6080 bp
• RefSeq ORF: 5934 bp
• Locus ID: 778
• UniProt ID: O60840
• Cytogenetics: Xp11.23
• Protein Families: Druggable Genome, Ion Channels: Calcium, Transmembrane
• Protein Pathways: Alzheimer's disease, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Calcium signaling pathway, Cardiac muscle contraction, Dilated cardiomyopathy, GnRH signaling pathway, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Vascular smooth muscle contraction
• MW: 220.5 kDa
• Gene Summary: This gene encodes a multipass transmembrane protein that functions as an alpha-1 subunit of the voltage-dependent calcium channel, which mediates the influx of calcium ions into the cell. The encoded protein forms a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Mutations in this gene can cause X-linked eye disorders, including congenital stationary night blindness type 2A, cone-rod dystropy, and Aland Island eye disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Aug 2013]