Cellular Apoptosis Susceptibility (CSE1L) (NM_001316) Human Tagged ORF Clone

CAT#: RC211478

CSE1L (Myc-DDK-tagged)-Human CSE1 chromosome segregation 1-like (yeast) (CSE1L)

Product Images

Circular map for RC211478

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  Western blot validation of overexpression lysate (Cat# LY420011) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211478 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CSE1L protein (Cat# TP311478). The protein was produced from HEK293T cells transfected with CSE1L cDNA clone (Cat# RC211478) using MegaTran 2.0 (Cat# TT210002).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CSE1L (Cat# RC211478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSE1L(Cat# TA812195). Positive lysates LY420011 (100ug) and LC420011 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cellular Apoptosis Susceptibility
• Synonyms: CAS; CSE1; XPO2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211478 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTCAGCGATGCAAATCTGCAAACACTAACAGAATATTTAAAGAAAACACTTGATCCTGATCCTG
  CCATCCGACGTCCAGCTGAGAAATTTCTTGAATCTGTTGAAGGAAATCAGAATTATCCACTGTTGCTTTT
  GACATTACTGGAGAAGTCCCAGGATAATGTTATCAAAGTATGTGCTTCAGTAACATTCAAAAACTATATT
  AAAAGGAACTGGAGAATTGTTGAAGATGAACCAAACAAAATTTGTGAAGCCGATCGAGTGGCCATTAAAG
  CCAACATAGTGCACTTGATGCTTAGCAGCCCAGAGCAAATTCAGAAGCAGTTAAGTGATGCAATTAGCAT
  TATTGGCAGAGAAGATTTTCCACAGAAATGGCCTGACTTGCTGACAGAAATGGTGAATCGCTTTCAGAGT
  GGAGATTTCCATGTTATTAATGGAGTCCTCCGTACAGCACATTCATTATTTAAAAGATACCGTCATGAAT
  TTAAGTCAAACGAGTTATGGACTGAAATTAAGCTTGTTCTGGATGCCTTTGCTTTGCCTTTGACTAATCT
  TTTTAAGGCCACTATTGAACTCTGCAGTACCCATGCAAATGATGCCTCTGCCCTGAGGATTCTGTTTTCT
  TCCCTGATCCTGATCTCAAAATTGTTCTATAGTTTAAACTTTCAGGATCTCCCTGAATTTTTTGAAGATA
  ATATGGAAACTTGGATGAATAATTTTCATACTCTCTTAACATTGGATAATAAGCTTTTACAAACTGATGA
  TGAAGAGGAAGCCGGCTTATTGGAGCTCTTAAAATCCCAGATTTGTGATAATGCCGCACTCTATGCACAA
  AAGTACGATGAAGAATTCCAGCGATACCTGCCTCGTTTTGTTACAGCCATCTGGAATTTACTAGTTACAA
  CGGGTCAAGAGGTTAAATATGATTTGTTGGTAAGTAATGCAATTCAATTTCTGGCTTCAGTTTGTGAGAG
  ACCTCATTATAAGAATCTATTTGAGGACCAGAACACGCTGACAAGTATCTGTGAAAAGGTTATTGTGCCT
  AACATGGAATTTAGAGCTGCTGATGAAGAAGCATTTGAAGATAATTCTGAGGAGTACATAAGGAGAGATT
  TGGAAGGATCTGATATTGATACTAGACGCAGGGCTGCTTGTGATCTGGTACGAGGATTATGCAAGTTTTT
  TGAGGGACCTGTGACAGGAATCTTCTCTGGTTATGTTAATTCCATGCTGCAGGAATACGCAAAAAATCCA
  TCTGTCAACTGGAAACACAAAGATGCAGCCATCTACCTAGTGACATCTTTGGCATCAAAAGCCCAAACAC
  AGAAGCATGGAATTACACAAGCAAATGAACTTGTAAACCTAACTGAGTTCTTTGTGAATCACATCCTCCC
  TGATTTAAAATCAGCTAATGTGAATGAATTTCCTGTCCTTAAAGCTGACGGTATCAAATATATTATGATT
  TTTAGAAATCAAGTGCCAAAAGAACATCTTTTAGTCTCGATTCCTCTCTTGATTAATCATCTTCAAGCTG
  AAAGTATTGTTGTTCATACTTACGCAGCTCATGCTCTTGAACGGCTCTTTACTATGCGAGGGCCTAACAA
  TGCCACTCTCTTTACAGCTGCAGAAATCGCACCGTTTGTTGAGATTCTGCTAACAAACCTTTTCAAAGCT
  CTCACACTTCCTGGCTCTTCAGAAAATGAATATATTATGAAAGCTATCATGAGAAGTTTTTCTCTCCTAC
  AAGAAGCCATAATCCCCTACATCCCTACTCTCATCACTCAGCTTACACAGAAGCTATTAGCTGTTAGTAA
  GAACCCAAGCAAACCTCACTTTAATCACTACATGTTTGAAGCAATATGTTTATCCATAAGAATAACTTGC
  AAAGCTAACCCTGCTGCTGTTGTAAATTTTGAGGAGGCTTTGTTTTTGGTGTTTACTGAAATCTTACAAA
  ATGATGTGCAAGAATTTATTCCATACGTCTTTCAAGTGATGTCTTTGCTTCTGGAAACACACAAAAATGA
  CATCCCGTCTTCCTATATGGCCTTATTTCCTCATCTCCTTCAGCCAGTGCTTTGGGAAAGAACAGGAAAT
  ATTCCTGCTCTAGTGAGGCTTCTTCAAGCATTCTTAGAACGCGGTTCAAACACAATAGCAAGTGCTGCAG
  CTGACAAAATTCCTGGGTTACTAGGTGTCTTTCAGAAGCTGATTGCATCCAAAGCAAATGACCACCAAGG
  TTTTTATCTTCTAAACAGTATAATAGAGCACATGCCTCCTGAATCAGTTGACCAATATAGGAAACAAATC
  TTCATTCTGCTATTCCAGAGACTTCAGAATTCCAAAACAACCAAGTTTATCAAGAGTTTTTTAGTCTTTA
  TTAATTTGTATTGCATAAAATATGGGGCACTAGCACTACAAGAAATATTTGATGGTATACAACCAAAAAT
  GTTTGGAATGGTTTTGGAAAAAATTATTATTCCTGAAATTCAGAAGGTATCTGGAAATGTAGAGAAAAAG
  ATCTGTGCGGTTGGCATAACCAAATTACTAACAGAATGTCCCCCAATGATGGACACTGAGTATACCAAAC
  TGTGGACTCCATTATTACAGTCTTTGATTGGTCTTTTTGAGTTACCCGAAGATGATACCATTCCTGATGA
  GGAACATTTTATTGACATAGAAGATACACCAGGATATCAGACTGCCTTCTCACAGTTGGCATTTGCTGGG
  AAAAAAGAGCATGATCCTGTAGGTCAAATGGTGAATAACCCCAAAATTCACCTGGCACAGTCACTTCACA
  AGTTGTCTACCGCCTGTCCAGGAAGGGTTCCATCAATGGTGAGCACCAGCCTGAATGCAGAAGCGCTCCA
  GTATCTCCAAGGGTACCTTCAGGCAGCCAGTGTGACACTGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211478 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELSDANLQTLTEYLKKTLDPDPAIRRPAEKFLESVEGNQNYPLLLLTLLEKSQDNVIKVCASVTFKNYI
  KRNWRIVEDEPNKICEADRVAIKANIVHLMLSSPEQIQKQLSDAISIIGREDFPQKWPDLLTEMVNRFQS
  GDFHVINGVLRTAHSLFKRYRHEFKSNELWTEIKLVLDAFALPLTNLFKATIELCSTHANDASALRILFS
  SLILISKLFYSLNFQDLPEFFEDNMETWMNNFHTLLTLDNKLLQTDDEEEAGLLELLKSQICDNAALYAQ
  KYDEEFQRYLPRFVTAIWNLLVTTGQEVKYDLLVSNAIQFLASVCERPHYKNLFEDQNTLTSICEKVIVP
  NMEFRAADEEAFEDNSEEYIRRDLEGSDIDTRRRAACDLVRGLCKFFEGPVTGIFSGYVNSMLQEYAKNP
  SVNWKHKDAAIYLVTSLASKAQTQKHGITQANELVNLTEFFVNHILPDLKSANVNEFPVLKADGIKYIMI
  FRNQVPKEHLLVSIPLLINHLQAESIVVHTYAAHALERLFTMRGPNNATLFTAAEIAPFVEILLTNLFKA
  LTLPGSSENEYIMKAIMRSFSLLQEAIIPYIPTLITQLTQKLLAVSKNPSKPHFNHYMFEAICLSIRITC
  KANPAAVVNFEEALFLVFTEILQNDVQEFIPYVFQVMSLLLETHKNDIPSSYMALFPHLLQPVLWERTGN
  IPALVRLLQAFLERGSNTIASAAADKIPGLLGVFQKLIASKANDHQGFYLLNSIIEHMPPESVDQYRKQI
  FILLFQRLQNSKTTKFIKSFLVFINLYCIKYGALALQEIFDGIQPKMFGMVLEKIIIPEIQKVSGNVEKK
  ICAVGITKLLTECPPMMDTEYTKLWTPLLQSLIGLFELPEDDTIPDEEHFIDIEDTPGYQTAFSQLAFAG
  KKEHDPVGQMVNNPKIHLAQSLHKLSTACPGRVPSMVSTSLNAEALQYLQGYLQAASVTLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001316
• ORF Size: 2913 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001316.4
• RefSeq Size: 3627 bp
• RefSeq ORF: 2916 bp
• Locus ID: 1434
• UniProt ID: P55060
• Cytogenetics: 20q13.13
• Domains: IBN_NT, CAS_CSE1
• Protein Families: Druggable Genome
• MW: 110.4 kDa
• Gene Summary: Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, the encoded protein may play a role both in apoptosis and in cell proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]