SWI5 (NM_001040011) Human Tagged ORF Clone

CAT#: RC211457

  • TrueORF®

SWI5 (Myc-DDK-tagged)-Human chromosome 9 open reading frame 119 (C9orf119)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "NM_001040011" in other vectors (4)

Reconstitution Protocol

USD 300.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SWI5
Synonyms C9orf119; SAE3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211457 representing NM_001040011
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCGGCGTGGCCAGAGGGACCTGTGGCGTCACAACAAAAGCTGCGCACGCAACCGCTGTCCCCGCC
CACCTCGGGAGAGGGGCGGGGCCGGCTTTCCTTGGGTGCGCGCGCAGCTTTCTGTGCGCCAGTTCACACT
CCGGGTCAGAGTTCCTGGCCCGGTGCACCTGAGAGGTCGCTCTCCGACTCCCGCGCTGGACCCTCTTGCG
CCATTGAACCCCCTGATCCGGGGGCCTCGGACCCCAGGGCTCAGGAGATGGATCCAGTCCCTGGCGCTCC
TACTTCCCAACTGCTCCTCCTCCCGCATCCCCACAGTACCCCGTCCTCACAGCGGGCTGTGGGTCCAATC
AGACTTTCCCCTCGGATTCCTCTCTAGGACTGAACCAAGACTTACCCGAAGTTGCCGCGGGGCCTTCCGA
TCCCCTAGGCCATTGCCCAAGTCTGGCCAGGCTGATGGGACCAGCGAGGAGTCTCTGCACCTTGACATTC
AGAAACTGAAGGAGAAGAGGGACATGCTGGACAAGGAGATCTCCCAGTTCGTATCTGAAGGCTACAGTGT
GGATGAACTGGAGGACCACATTACCCAGCTTCACGAGTACAATGACATCAAGGATGTGGGGCAGATGCTG
ATGGGCAAACTAGCTGTGATCCGAGGTGTCACCACCAAAGAGTTGTATCCAGAGTTTGGGCTGGACATGA
ATGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211457 representing NM_001040011
Red=Cloning site Green=Tags(s)

MQRRGQRDLWRHNKSCARNRCPRPPRERGGAGFPWVRAQLSVRQFTLRVRVPGPVHLRGRSPTPALDPLA
PLNPLIRGPRTPGLRRWIQSLALLLPNCSSSRIPTVPRPHSGLWVQSDFPLGFLSRTEPRLTRSCRGAFR
SPRPLPKSGQADGTSEESLHLDIQKLKEKRDMLDKEISQFVSEGYSVDELEDHITQLHEYNDIKDVGQML
MGKLAVIRGVTTKELYPEFGLDMND

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001040011
ORF Size 705 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001040011.1, NP_001035100.1
RefSeq Size 1004 bp
RefSeq ORF 513 bp
Locus ID 375757
UniProt ID Q1ZZU3
Cytogenetics 9q34.11
MW 26.6 kDa
Gene Summary Component of the SWI5-SFR1 complex, a complex required for double-strand break repair via homologous recombination.[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.