ARID5B (NM_032199) Human Tagged ORF Clone

CAT#: RC211454

ARID5B (Myc-DDK-tagged)-Human AT rich interactive domain 5B (MRF1-like) (ARID5B)

Product Images

Western blot validation of overexpression lysate (Cat# LY410288) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211454 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARID5B
• Synonyms: DESRT; MRF-2; MRF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211454 representing NM_032199
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCAACTCACTCCAGTGGGTCGGCTCACCGTGTGGCTTGCACGGACCTTACATTTTCTACAAGG
  CTTTTCAATTCCACCTTGAAGGCAAACCAAGAATTTTGTCCCTTGGCGACTTTTTCTTTGTAAGATGTAC
  GCCAAAGGATCCGATTTGCATAGCGGAGCTCCAGCTGTTGTGGGAAGAGAGGACCAGCCGGCAACTTTTA
  TCCAGCTCTAAACTTTATTTCCTCCCAGAAGACACTCCCCAGGGCAGAAATAGCGACCATGGCGAGGATG
  AAGTCATTGCTGTTTCCGAAAAGGTGATTGTGAAGCTTGAAGACCTGGTCAAGTGGGTACATTCTGATTT
  CTCCAAGTGGAGATGTGGCTTCCACGCTGGACCAGTGAAAACTGAGGCCTTGGGAAGGAATGGACAGAAG
  GAAGCTCTGCTGAAGTACAGGCAGTCAACCCTAAACAGTGGACTCAACTTCAAAGACGTTCTCAAGGAGA
  AGGCAGACCTGGGGGAGGACGAGGAAGAAACGAACGTGATAGTTCTCAGCTACCCCCAGTACTGCCGGTA
  CCGCTCGATGCTGAAACGCATCCAGGATAAGCCATCTTCCATTCTAACGGACCAGTTTGCATTGGCCCTG
  GGGGGCATTGCAGTGGTCAGCAGGAACCCTCAGATCCTGTACTGTCGGGACACCTTTGACCACCCGACTC
  TCATAGAAAACGAGAGTATATGCGATGAGTTTGCGCCAAATCTTAAAGGCAGACCACGCAAAAAGAAACC
  ATGCCCACAAAGAAGAGATTCATTCAGTGGTGTTAAGGATTCCAACAACAATTCCGATGGCAAAGCCGTT
  GCCAAGGTGAAATGTGAGGCCAGGTCAGCCTTGACCAAGCCGAAGAATAACCATAACTGTAAAAAAGTCT
  CAAATGAAGAAAAACCAAAGGTTGCCATTGGTGAAGAGTGCAGGGCAGATGAACAAGCCTTCTTGGTGGC
  ACTTTATAAATACATGAAAGAAAGGAAAACGCCGATAGAACGAATACCCTATTTAGGTTTTAAACAGATT
  AACCTTTGGACTATGTTTCAAGCTGCTCAAAAACTGGGAGGATATGAAACAATAACAGCCCGCCGTCAGT
  GGAAACATATTTATGATGAATTAGGCGGTAATCCTGGGAGCACCAGCGCTGCCACTTGTACCCGCAGACA
  TTATGAAAGATTAATCCTACCATATGAAAGATTTATTAAAGGAGAAGAAGATAAGCCCCTGCCTCCAATC
  AAACCTCGGAAACAGGAGAACAGTTCACAGGAAAATGAGAACAAAACAAAAGTATCTGGAACCAAACGCA
  TCAAACATGAAATACCTAAAAGCAAGAAAGAAAAAGAAAATGCCCCAAAGCCCCAGGATGCAGCAGAGGT
  TTCATCAGAGCAAGAAAAAGAACAAGAGACTTTAATAAGCCAGAAAAGCATCCCTGAGCCTCTCCCAGCA
  GCAGACATGAAGAAAAAAATAGAAGGGTATCAGGAATTTTCAGCGAAGCCCCTGGCATCCAGAGTAGACC
  CAGAGAAGGACAACGAAACAGACCAAGGTTCCAACAGTGAGAAGGTGGCAGAGGAGGCGGGAGAGAAGGG
  GCCCACACCTCCACTCCCAAGTGCTCCTCTGGCCCCAGAAAAAGATTCAGCCTTGGTCCCTGGGGCCAGC
  AAACAGCCACTCACCTCTCCTAGTGCCCTGGTGGACTCAAAACAAGAATCCAAACTGTGCTGTTTTACAG
  AGAGCCCTGAAAGTGAACCCCAAGAAGCATCCTTCCCCAGCTTCCCCACCACACAGCCACCGCTGGCAAA
  CCAGAATGAGACGGAGGATGACAAACTGCCCGCCATGGCAGATTACATTGCCAACTGCACCGTGAAGGTG
  GACCAGCTGGGCAGTGACGACATCCACAATGCGCTCAAGCAGACCCCAAAGGTCCTTGTGGTCCAGTCGT
  TTGACATGTTCAAAGACAAAGACCTGACTGGGCCCATGAACGAGAACCATGGACTTAATTACACGCCCCT
  GCTCTACTCTAGGGGCAACCCAGGCATCATGTCCCCACTGGCCAAGAAAAAGCTTTTGTCCCAAGTGAGT
  GGGGCCAGCCTCTCCAGCAGCTACCCTTATGGCTCCCCACCCCCTTTGATCAGCAAAAAGAAACTGATTG
  CTAGGGATGACTTGTGTTCCAGTTTGTCCCAGACCCACCATGGCCAAAGCACTGACCATATGGCGGTCAG
  CCGGCCATCAGTGATTCAGCACGTCCAGAGTTTCAGAAGCAAGCCCTCGGAAGAGAGAAAGACCATCAAT
  GACATCTTTAAGCATGAGAAACTGAGTCGATCAGATCCCCACCGCTGCAGCTTCTCCAAGCATCACCTTA
  ACCCCCTTGCTGACTCCTACGTCCTGAAGCAAGAAATTCAGGAGGGCAAGGATAAACTCTTAGAGAAAAG
  GGCCCTCCCCCATTCCCACATGCCTAGCTTCCTGGCTGACTTCTACTCGTCCCCTCATCTCCATAGCCTC
  TACAGACACACCGAGCACCATCTTCATAATGAACAGACATCCAAATACCCTTCCAGGGACATGTACAGGG
  AATCGGAAAACAGTTCTTTTCCTTCCCACAGACACCAAGAAAAGCTCCATGTAAATTATCTCACGTCCCT
  GCACCTGCAAGACAAAAAGTCGGCGGCAGCAGAAGCCCCTACGGATGATCAGCCTACAGATCTGAGCCTT
  CCCAAGAACCCGCACAAACCTACCGGCAAGGTCCTGGGCCTGGCTCATTCCACCACAGGGCCCCAGGAGA
  GCAAAGGCATCTCCCAGTTCCAGGTCTTAGGCAGCCAGAGTCGAGACTGTCACCCCAAAGCCTGTCGGGT
  ATCACCCATGACCATGTCAGGCCCTAAAAAATACCCTGAATCGCTTTCAAGATCAGGAAAACCTCACCAT
  GTGAGACTGGAGAATTTCAGGAAGATGGAAGGCATGGTCCACCCAATCCTGCACCGGAAAATGAGCCCGC
  AGAACATTGGGGCGGCGCGGCCGATCAAGCGCAGCCTGGAGGATTTGGACCTTGTGATTGCAGGGAAAAA
  GGCCCGGGCAGTGTCTCCCTTAGACCCATCCAAGGAGGTCTCTGGGAAGGAGAAGGCCTCTGAGCAGGAG
  AGTGAAGGCAGCAAAGCAGCGCACGGTGGGCATTCCGGGGGCGGATCAGAAGGCCACAAGCTTCCCCTCT
  CCTCCCCTATCTTCCCAGGTCTGTATTCCGGGAGCCTGTGTAACTCGGGCCTCAACTCCAGGCTCCCGGC
  TGGGTATTCTCATTCTCTGCAGTACTTGAAAAACCAGACTGTGCTTTCTCCACTCATGCAGCCCCTGGCT
  TTCCACTCGCTTGTGATGCAAAGAGGAATTTTTACATCACCGACAAATTCTCAGCAGCTGTACAGACACT
  TGGCTGCGGCTACACCTGTAGGAAGTTCATATGGGGACCTTTTGCATAACAGCATTTACCCTTTAGCTGC
  TATAAATCCTCAAGCTGCCTTTCCATCTTCCCAGCTGTCATCCGTGCACCCCAGTACAAAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211454 representing NM_032199
  Red=Cloning site Green=Tags(s)
  
  MEPNSLQWVGSPCGLHGPYIFYKAFQFHLEGKPRILSLGDFFFVRCTPKDPICIAELQLLWEERTSRQLL
  SSSKLYFLPEDTPQGRNSDHGEDEVIAVSEKVIVKLEDLVKWVHSDFSKWRCGFHAGPVKTEALGRNGQK
  EALLKYRQSTLNSGLNFKDVLKEKADLGEDEEETNVIVLSYPQYCRYRSMLKRIQDKPSSILTDQFALAL
  GGIAVVSRNPQILYCRDTFDHPTLIENESICDEFAPNLKGRPRKKKPCPQRRDSFSGVKDSNNNSDGKAV
  AKVKCEARSALTKPKNNHNCKKVSNEEKPKVAIGEECRADEQAFLVALYKYMKERKTPIERIPYLGFKQI
  NLWTMFQAAQKLGGYETITARRQWKHIYDELGGNPGSTSAATCTRRHYERLILPYERFIKGEEDKPLPPI
  KPRKQENSSQENENKTKVSGTKRIKHEIPKSKKEKENAPKPQDAAEVSSEQEKEQETLISQKSIPEPLPA
  ADMKKKIEGYQEFSAKPLASRVDPEKDNETDQGSNSEKVAEEAGEKGPTPPLPSAPLAPEKDSALVPGAS
  KQPLTSPSALVDSKQESKLCCFTESPESEPQEASFPSFPTTQPPLANQNETEDDKLPAMADYIANCTVKV
  DQLGSDDIHNALKQTPKVLVVQSFDMFKDKDLTGPMNENHGLNYTPLLYSRGNPGIMSPLAKKKLLSQVS
  GASLSSSYPYGSPPPLISKKKLIARDDLCSSLSQTHHGQSTDHMAVSRPSVIQHVQSFRSKPSEERKTIN
  DIFKHEKLSRSDPHRCSFSKHHLNPLADSYVLKQEIQEGKDKLLEKRALPHSHMPSFLADFYSSPHLHSL
  YRHTEHHLHNEQTSKYPSRDMYRESENSSFPSHRHQEKLHVNYLTSLHLQDKKSAAAEAPTDDQPTDLSL
  PKNPHKPTGKVLGLAHSTTGPQESKGISQFQVLGSQSRDCHPKACRVSPMTMSGPKKYPESLSRSGKPHH
  VRLENFRKMEGMVHPILHRKMSPQNIGAARPIKRSLEDLDLVIAGKKARAVSPLDPSKEVSGKEKASEQE
  SEGSKAAHGGHSGGGSEGHKLPLSSPIFPGLYSGSLCNSGLNSRLPAGYSHSLQYLKNQTVLSPLMQPLA
  FHSLVMQRGIFTSPTNSQQLYRHLAAATPVGSSYGDLLHNSIYPLAAINPQAAFPSSQLSSVHPSTKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032199
• ORF Size: 3564 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032199.3
• RefSeq Size: 7507 bp
• RefSeq ORF: 3567 bp
• Locus ID: 84159
• UniProt ID: Q14865
• Cytogenetics: 10q21.2
• MW: 132.2 kDa
• Gene Summary: This gene encodes a member of the AT-rich interaction domain (ARID) family of DNA binding proteins. The encoded protein forms a histone H3K9Me2 demethylase complex with PHD finger protein 2 and regulates the transcription of target genes involved in adipogenesis and liver development. This gene also plays a role in cell growth and differentiation of B-lymphocyte progenitors, and single nucleotide polymorphisms in this gene are associated with acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]