HIP1 (NM_005338) Human Tagged ORF Clone

CAT#: RC211418

HIP1 (Myc-DDK-tagged)-Human huntingtin interacting protein 1 (HIP1)

Product Images

Circular map for RC211418

• 

  Western blot validation of overexpression lysate (Cat# LY417370) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211418 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HIP1
• Synonyms: HIP-I; ILWEQ; SHON; SHONbeta; SHONgamma
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211418 representing NM_005338
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCGGATGGCCAGCTCCATGAAGCAGGTGCCCAACCCACTGCCCAAGGTGCTGAGCCGGCGCGGGG
  TCGGCGCTGGGCTGGAGGCGGCGGAGCGCGAGAGCTTCGAGCGGACTCAGACTGTCAGCATCAATAAGGC
  CATTAATACGCAGGAAGTGGCTGTAAAGGAAAAACACGCCAGAACGTGCATACTGGGCACCCACCATGAG
  AAAGGGGCACAGACCTTCTGGTCTGTTGTCAACCGCCTGCCTCTGTCTAGCAACGCAGTGCTCTGCTGGA
  AGTTCTGCCATGTGTTCCACAAACTCCTCCGAGATGGACACCCGAACGTCCTGAAGGACTCTCTGAGATA
  CAGAAATGAATTGAGTGACATGAGCAGGATGTGGGGCCACCTGAGCGAGGGGTATGGCCAGCTGTGCAGC
  ATCTACCTGAAACTGCTAAGAACCAAGATGGAGTACCACACCAAAAATCCCAGGTTCCCAGGCAACCTGC
  AGATGAGTGACCGCCAGCTGGACGAGGCTGGAGAAAGTGACGTGAACAACTTTTTCCAGTTAACAGTGGA
  GATGTTTGACTACCTGGAGTGTGAACTCAACCTCTTCCAAACAGTATTCAACTCCCTGGACATGTCCCGC
  TCTGTGTCCGTGACGGCAGCAGGGCAGTGCCGCCTCGCCCCGCTGATCCAGGTCATCTTGGACTGCAGCC
  ACCTTTATGACTACACTGTCAAGCTTCTCTTCAAACTCCACTCCTGCCTCCCAGCTGACACCCTGCAAGG
  CCACCGGGACCGCTTCATGGAGCAGTTTACAAAGTTGAAAGATCTGTTCTACCGCTCCAGCAACCTGCAG
  TACTTCAAGCGGCTCATTCAGATCCCCCAGCTGCCTGAGAACCCACCCAACTTCCTGCGAGCCTCAGCCC
  TGTCAGAACATATCAGCCCTGTGGTGGTGATCCCTGCAGAGGCCTCATCCCCCGACAGCGAGCCAGTCCT
  AGAGAAGGATGACCTCATGGACATGGATGCCTCTCAGCAGAATTTATTTGACAACAAGTTTGATGACATC
  TTTGGCAGTTCATTCAGCAGTGATCCCTTCAATTTCAACAGTCAAAATGGTGTGAACAAGGATGAGAAGG
  ACCACTTAATTGAGCGACTATACAGAGAGATCAGTGGATTGAAGGCACAGCTAGAAAACATGAAGACTGA
  GAGCCAGCGGGTTGTGCTGCAGCTGAAGGGCCACGTCAGCGAGCTGGAAGCAGATCTGGCCGAGCAGCAG
  CACCTGCGGCAGCAGGCGGCCGACGACTGTGAATTCCTGCGGGCAGAACTGGACGAGCTCAGGAGGCAGC
  GGGAGGACACCGAGAAGGCTCAGCGGAGCCTGTCTGAGATAGAAAGGAAAGCTCAAGCCAATGAACAGCG
  ATATAGCAAGCTAAAGGAGAAGTACAGCGAGCTGGTTCAGAACCACGCTGACCTGCTGCGGAAGAATGCA
  GAGGTGACCAAACAGGTGTCCATGGCCAGACAAGCCCAGGTAGATTTGGAACGAGAGAAAAAAGAGCTGG
  AGGATTCGTTGGAGCGCATCAGTGACCAGGGCCAGCGGAAGACTCAAGAACAGCTGGAAGTTCTAGAGAG
  CTTGAAGCAGGAACTTGCCACAAGCCAACGGGAGCTTCAGGTTCTGCAAGGCAGCCTGGAAACTTCTGCC
  CAGTCAGAAGCAAACTGGGCAGCCGAGTTCGCCGAGCTAGAGAAGGAGCGGGACAGCCTGGTGAGTGGCG
  CAGCTCATAGGGAGGAGGAATTATCTGCTCTTCGGAAAGAACTGCAGGACACTCAGCTCAAACTGGCCAG
  CACAGAGGAATCTATGTGCCAGCTTGCCAAAGACCAACGAAAAATGCTTCTGGTGGGGTCCAGGAAGGCT
  GCGGAGCAGGTGATACAAGACGCCCTGAACCAGCTTGAAGAACCTCCTCTCATCAGCTGCGCTGGGTCTG
  CAGATCACCTCCTCTCCACGGTCACATCCATTTCCAGCTGCATCGAGCAACTGGAGAAAAGCTGGAGCCA
  GTATCTGGCCTGCCCAGAAGACATCAGTGGACTTCTCCATTCCATAACCCTGCTGGCCCACTTGACCAGC
  GACGCCATTGCTCATGGTGCCACCACCTGCCTCAGAGCCCCACCTGAGCCTGCCGACTCACTGACCGAGG
  CCTGTAAGCAGTATGGCAGGGAAACCCTCGCCTACCTGGCCTCCCTGGAGGAAGAGGGAAGCCTTGAGAA
  TGCCGACAGCACAGCCATGAGGAACTGCCTGAGCAAGATCAAGGCCATCGGCGAGGAGCTCCTGCCCAGG
  GGACTGGACATCAAGCAGGAGGAGCTGGGGGACCTGGTGGACAAGGAGATGGCGGCCACTTCAGCTGCTA
  TTGAAACTGCCACGGCCAGAATAGAGGAGATGCTCAGCAAATCCCGAGCAGGAGACACAGGAGTCAAATT
  GGAGGTGAATGAAAGGATCCTTGGTTGCTGTACCAGCCTCATGCAAGCTATTCAGGTGCTCATCGTGGCC
  TCTAAGGACCTCCAGAGAGAGATTGTGGAGAGCGGCAGGGGTACAGCATCCCCTAAAGAGTTTTATGCCA
  AGAACTCTCGATGGACAGAAGGACTTATCTCAGCCTCCAAGGCTGTGGGCTGGGGAGCCACTGTCATGGT
  GGATGCAGCTGATCTGGTGGTACAAGGCAGAGGGAAATTTGAGGAGCTAATGGTGTGTTCTCATGAAATT
  GCTGCTAGCACAGCCCAGCTTGTGGCTGCATCCAAGGTGAAAGCTGATAAGGACAGCCCCAACCTAGCCC
  AGCTGCAGCAGGCCTCTCGGGGAGTGAACCAGGCCACTGCCGGCGTTGTGGCCTCAACCATTTCCGGCAA
  ATCACAGATCGAAGAGACAGACAACATGGACTTCTCAAGCATGACGCTGACACAGATCAAACGCCAAGAG
  ATGGATTCTCAGGTTAGGGTGCTAGAGCTAGAAAATGAATTGCAGAAGGAGCGTCAAAAACTGGGAGAGC
  TTCGGAAAAAGCACTACGAGCTTGCTGGTGTTGCTGAGGGCTGGGAAGAAGGAACAGAGGCATCTCCACC
  TACACTGCAAGAAGTGGTAACCGAAAAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211418 representing NM_005338
  Red=Cloning site Green=Tags(s)
  
  MDRMASSMKQVPNPLPKVLSRRGVGAGLEAAERESFERTQTVSINKAINTQEVAVKEKHARTCILGTHHE
  KGAQTFWSVVNRLPLSSNAVLCWKFCHVFHKLLRDGHPNVLKDSLRYRNELSDMSRMWGHLSEGYGQLCS
  IYLKLLRTKMEYHTKNPRFPGNLQMSDRQLDEAGESDVNNFFQLTVEMFDYLECELNLFQTVFNSLDMSR
  SVSVTAAGQCRLAPLIQVILDCSHLYDYTVKLLFKLHSCLPADTLQGHRDRFMEQFTKLKDLFYRSSNLQ
  YFKRLIQIPQLPENPPNFLRASALSEHISPVVVIPAEASSPDSEPVLEKDDLMDMDASQQNLFDNKFDDI
  FGSSFSSDPFNFNSQNGVNKDEKDHLIERLYREISGLKAQLENMKTESQRVVLQLKGHVSELEADLAEQQ
  HLRQQAADDCEFLRAELDELRRQREDTEKAQRSLSEIERKAQANEQRYSKLKEKYSELVQNHADLLRKNA
  EVTKQVSMARQAQVDLEREKKELEDSLERISDQGQRKTQEQLEVLESLKQELATSQRELQVLQGSLETSA
  QSEANWAAEFAELEKERDSLVSGAAHREEELSALRKELQDTQLKLASTEESMCQLAKDQRKMLLVGSRKA
  AEQVIQDALNQLEEPPLISCAGSADHLLSTVTSISSCIEQLEKSWSQYLACPEDISGLLHSITLLAHLTS
  DAIAHGATTCLRAPPEPADSLTEACKQYGRETLAYLASLEEEGSLENADSTAMRNCLSKIKAIGEELLPR
  GLDIKQEELGDLVDKEMAATSAAIETATARIEEMLSKSRAGDTGVKLEVNERILGCCTSLMQAIQVLIVA
  SKDLQREIVESGRGTASPKEFYAKNSRWTEGLISASKAVGWGATVMVDAADLVVQGRGKFEELMVCSHEI
  AASTAQLVAASKVKADKDSPNLAQLQQASRGVNQATAGVVASTISGKSQIEETDNMDFSSMTLTQIKRQE
  MDSQVRVLELENELQKERQKLGELRKKHYELAGVAEGWEEGTEASPPTLQEVVTEKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005338
• ORF Size: 3111 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005338.7
• RefSeq Size: 8051 bp
• RefSeq ORF: 3114 bp
• Locus ID: 3092
• UniProt ID: O00291
• Cytogenetics: 7q11.23
• Domains: I_LWEQ
• Protein Families: Druggable Genome
• Protein Pathways: Huntington's disease
• MW: 116.2 kDa
• Gene Summary: The product of this gene is a membrane-associated protein that functions in clathrin-mediated endocytosis and protein trafficking within the cell. The encoded protein binds to the huntingtin protein in the brain; this interaction is lost in Huntington's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]