FOXA2 (NM_153675) Human Tagged ORF Clone

CAT#: RC211408

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FOXA2 (Myc-DDK-tagged)-Human forkhead box A2 (FOXA2), transcript variant 2



  "NM_153675" in other vectors (6)

Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol FOXA2
Synonyms HNF-3-beta; HNF3B; TCF3B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211408 representing NM_153675
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGGAGCGGTGAAGATGGAAGGGCACGAGCCGTCCGACTGGAGCAGCTACTATGCAGAGCCCGAGG
GCTACTCCTCCGTGAGCAACATGAACGCCGGCCTGGGGATGAACGGCATGAACACGTACATGAGCATGTC
GGCGGCCGCCATGGGCAGCGGCTCGGGCAACATGAGCGCGGGCTCCATGAACATGTCGTCGTACGTGGGC
GCTGGCATGAGCCCGTCCCTGGCGGGGATGTCCCCCGGCGCGGGCGCCATGGCGGGCATGGGCGGCTCGG
CCGGGGCGGCTGGCGTGGCGGGCATGGGGCCGCACTTGAGTCCCAGCCTGAGCCCGCTCGGGGGGCAGGC
GGCCGGGGCCATGGGCGGCCTGGCCCCCTACGCCAACATGAACTCCATGAGCCCCATGTACGGGCAGGCG
GGCCTGAGCCGCGCCCGCGACCCCAAGACCTACAGGCGCAGCTACACGCACGCAAAGCCGCCCTACTCGT
ACATCTCGCTCATCACCATGGCCATCCAGCAGAGCCCCAACAAGATGCTGACGCTGAGCGAGATCTACCA
GTGGATCATGGACCTCTTCCCCTTCTACCGGCAGAACCAGCAGCGCTGGCAGAACTCCATCCGCCACTCG
CTCTCCTTCAACGACTGTTTCCTGAAGGTGCCCCGCTCGCCCGACAAGCCCGGCAAGGGCTCCTTCTGGA
CCCTGCACCCTGACTCGGGCAACATGTTCGAGAACGGCTGCTACCTGCGCCGCCAGAAGCGCTTCAAGTG
CGAGAAGCAGCTGGCGCTGAAGGAGGCCGCAGGCGCCGCCGGCAGCGGCAAGAAGGCGGCCGCCGGGGCC
CAGGCCTCACAGGCTCAACTCGGGGAGGCCGCCGGGCCGGCCTCCGAGACTCCGGCGGGCACCGAGTCGC
CTCACTCGAGCGCCTCCCCGTGCCAGGAGCACAAGCGAGGGGGCCTGGGAGAGCTGAAGGGGACGCCGGC
TGCGGCGCTGAGCCCCCCAGAGCCGGCGCCCTCTCCCGGGCAGCAGCAGCAGGCCGCGGCCCACCTGCTG
GGCCCGCCCCACCACCCGGGCCTGCCGCCTGAGGCCCACCTGAAGCCGGAACACCACTACGCCTTCAACC
ACCCGTTCTCCATCAACAACCTCATGTCCTCGGAGCAGCAGCACCACCACAGCCACCACCACCACCAGCC
CCACAAAATGGACCTCAAGGCCTACGAACAGGTGATGCACTACCCCGGCTACGGTTCCCCCATGCCTGGC
AGCTTGGCCATGGGCCCGGTCACGAACAAAACGGGCCTGGACGCCTCGCCCCTGGCCGCAGATACCTCCT
ACTACCAGGGGGTGTACTCCCGGCCCATTATGAACTCCTCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211408 representing NM_153675
Red=Cloning site Green=Tags(s)

MLGAVKMEGHEPSDWSSYYAEPEGYSSVSNMNAGLGMNGMNTYMSMSAAAMGSGSGNMSAGSMNMSSYVG
AGMSPSLAGMSPGAGAMAGMGGSAGAAGVAGMGPHLSPSLSPLGGQAAGAMGGLAPYANMNSMSPMYGQA
GLSRARDPKTYRRSYTHAKPPYSYISLITMAIQQSPNKMLTLSEIYQWIMDLFPFYRQNQQRWQNSIRHS
LSFNDCFLKVPRSPDKPGKGSFWTLHPDSGNMFENGCYLRRQKRFKCEKQLALKEAAGAAGSGKKAAAGA
QASQAQLGEAAGPASETPAGTESPHSSASPCQEHKRGGLGELKGTPAAALSPPEPAPSPGQQQQAAAHLL
GPPHHPGLPPEAHLKPEHHYAFNHPFSINNLMSSEQQHHHSHHHHQPHKMDLKAYEQVMHYPGYGSPMPG
SLAMGPVTNKTGLDASPLAADTSYYQGVYSRPIMNSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_153675
ORF Size 1371 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_153675.3
RefSeq Size 2230 bp
RefSeq ORF 1374 bp
Locus ID 3170
UniProt ID Q9Y261
Cytogenetics 20p11.21
Protein Families Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors
Protein Pathways Maturity onset diabetes of the young
MW 48.1 kDa
Gene Summary This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]

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