NRG1 (NM_013962) Human Tagged ORF Clone

CAT#: RC211372

NRG1 (Myc-DDK-tagged)-Human neuregulin 1 (NRG1), transcript variant GGF2

Product Images

Circular map for RC211372

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NRG1
• Synonyms: ARIA; GGF; GGF2; HGL; HRG; HRG1; HRGA; MST131; MSTP131; NDF; NRG1-IT2; SMDF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211372 representing NM_013962
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGAGATGGCGACGCGCCCCGCGCCGCTCCGGGCGTCCCGGCCCCCGGGCCCAGCGCCCCGGCTCCGCCG
  CCCGCTCGTCGCCGCCGCTGCCGCTGCTGCCACTACTGCTGCTGCTGGGGACCGCGGCCCTGGCGCCGGG
  GGCGGCGGCCGGCAACGAGGCGGCTCCCGCGGGGGCCTCGGTGTGCTACTCGTCCCCGCCCAGCGTGGGA
  TCGGTGCAGGAGCTAGCTCAGCGCGCCGCGGTGGTGATCGAGGGAAAGGTGCACCCGCAGCGGCGGCAGC
  AGGGGGCACTCGACAGGAAGGCGGCGGCGGCGGCGGGCGAGGCAGGGGCGTGGGGCGGCGATCGCGAGCC
  GCCAGCCGCGGGCCCACGGGCGCTGGGGCCGCCCGCCGAGGAGCCGCTGCTCGCCGCCAACGGGACCGTG
  CCCTCTTGGCCCACCGCCCCGGTGCCCAGCGCCGGCGAGCCCGGGGAGGAGGCGCCCTATCTGGTGAAGG
  TGCACCAGGTGTGGGCGGTGAAAGCCGGGGGCTTGAAGAAGGACTCGCTGCTCACCGTGCGCCTGGGGAC
  CTGGGGCCACCCCGCCTTCCCCTCCTGCGGGAGGCTCAAGGAGGACAGCAGGTACATCTTCTTCATGGAG
  CCCGACGCCAACAGCACCAGCCGCGCGCCGGCCGCCTTCCGAGCCTCTTTCCCCCCTCTGGAGACGGGCC
  GGAACCTCAAGAAGGAGGTCAGCCGGGTGCTGTGCAAGCGGTGCGCCTTGCCTCCCCAATTGAAAGAGAT
  GAAAAGCCAGGAATCGGCTGCAGGTTCCAAACTAGTCCTTCGGTGTGAAACCAGTTCTGAATACTCCTCT
  CTCAGATTCAAGTGGTTCAAGAATGGGAATGAATTGAATCGAAAAAACAAACCACAAAATATCAAGATAC
  AAAAAAAGCCAGGGAAGTCAGAACTTCGCATTAACAAAGCATCACTGGCTGATTCTGGAGAGTATATGTG
  CAAAGTGATCAGCAAATTAGGAAATGACAGTGCCTCTGCCAATATCACCATCGTGGAATCAAACGCTACA
  TCTACATCCACCACTGGGACAAGCCATCTTGTAAAATGTGCGGAGAAGGAGAAAACTTTCTGTGTGAATG
  GAGGGGAGTGCTTCATGGTGAAAGACCTTTCAAACCCCTCGAGATACTTGTGCAAGTGCCCAAATGAGTT
  TACTGGTGATCGCTGCCAAAACTACGTAATGGCCAGCTTCTACAGTACGTCCACTCCCTTTCTGTCTCTG
  CCTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211372 representing NM_013962
  Red=Cloning site Green=Tags(s)
  
  MRWRRAPRRSGRPGPRAQRPGSAARSSPPLPLLPLLLLLGTAALAPGAAAGNEAAPAGASVCYSSPPSVG
  SVQELAQRAAVVIEGKVHPQRRQQGALDRKAAAAAGEAGAWGGDREPPAAGPRALGPPAEEPLLAANGTV
  PSWPTAPVPSAGEPGEEAPYLVKVHQVWAVKAGGLKKDSLLTVRLGTWGHPAFPSCGRLKEDSRYIFFME
  PDANSTSRAPAAFRASFPPLETGRNLKKEVSRVLCKRCALPPQLKEMKSQESAAGSKLVLRCETSSEYSS
  LRFKWFKNGNELNRKNKPQNIKIQKKPGKSELRINKASLADSGEYMCKVISKLGNDSASANITIVESNAT
  STSTTGTSHLVKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCPNEFTGDRCQNYVMASFYSTSTPFLSL
  PE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013962
• ORF Size: 1266 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013962.1, NP_039256.1
• RefSeq Size: 1986 bp
• RefSeq ORF: 1269 bp
• Locus ID: 3084
• UniProt ID: Q02297
• Cytogenetics: 8p12
• Protein Families: Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
• Protein Pathways: ErbB signaling pathway
• MW: 45 kDa
• Gene Summary: The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. An extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are expressed in a tissue-specific manner and differ significantly in their structure, and are classified as types I, II, III, IV, V and VI. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). [provided by RefSeq, Apr 2016]