Pumilio 2 (PUM2) (NM_015317) Human Tagged ORF Clone

CAT#: RC211307

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PUM2 (Myc-DDK-tagged)-Human pumilio homolog 2 (Drosophila) (PUM2)



  "NM_015317" in other vectors (6)

Reconstitution Protocol

USD 926.00


Availability*
In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PUM2
Synonyms PUMH2; PUML2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211307 representing NM_015317
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCATGATTTTCAAGCTCTTGCATTAGAATCTCGGGGAATGGGAGAGCTTTTGCCTACCAAAAAGT
TTTGGGAACCTGATGATTCAACAAAAGATGGACAAAAAGGCATATTTCTTGGGGATGATGAATGGAGAGA
GACTGCATGGGGAGCTTCTCACCATTCAATGTCCCAGCCTATTATGGTACAGAGAAGATCTGGACAGGGT
TTTCATGGAAACAGTGAAGTAAATGCAATACTGTCTCCGCGATCAGAAAGTGGAGGCCTTGGTGTGAGCA
TGGTAGAATATGTATTAAGTTCTTCTCCTGCTGATAAATTGGATTCTCGATTTAGGAAGGGAAATTTTGG
CACTAGAGATGCTGAAACAGATGGACCTGAGAAAGGAGATCAAAAAGGCAAGGCTTCTCCATTTGAGGAG
GACCAAAACAGAGATCTTAAACAAGGAGATGATGATGATTCTAAAATAAATGGCAGAGGTTTGCCAAATG
GAATGGATGCCGATTGCAAAGATTTTAATCGTACTCCTGGAAGTCGTCAAGCCTCTCCAACTGAAGTAGT
TGAGCGCTTGGGCCCCAATACTAATCCCTCAGAAGGACTGGGGCCTCTTCCTAATCCTACAGCTAATAAA
CCACTTGTTGAAGAATTTTCAAATCCTGAAACTCAGAATCTGGATGCCATGGAACAAGTTGGTCTGGAAT
CCTTACAGTTTGACTATCCTGGTAATCAGGTACCAATGGACTCTTCAGGAGCTACTGTAGGCCTTTTTGA
CTACAATTCCCAGCAGCAGCTCTTTCAGAGGACTAATGCACTAACAGTTCAACAGTTAACTGCAGCTCAA
CAGCAGCAATATGCATTAGCAGCAGCTCAGCAGCCACATATAGCTGGTGTATTCTCAGCAGGCCTTGCTC
CAGCTGCATTTGTGCCAAATCCATACATTATTAGTGCTGCTCCTCCAGGGACCGATCCGTATACTGCAGC
AGGATTGGCTGCAGCAGCTACATTAGCAGGTCCAGCAGTGGTTCCACCTCAGTATTACGGCGTTCCATGG
GGGGTGTATCCAGCCAACTTATTTCAGCAGCAAGCTGCAGCTGCGGCAAATAACACAGCCAGTCAGCAAG
CAGCATCACAAGCTCAGCCTGGACAGCAACAGGTTCTCCGTGCTGGAGCAGGTCAGCGTCCTCTTACTCC
CAATCAGGGTCAGCAAGGGCAGCAAGCAGAATCACTTGCGGCAGCTGCAGCAGCAAATCCAACATTGGCT
TTTGGTCAGGGTCTTGCTACTGGCATGCCAGGCTATCAAGTACTAGCTCCAACTGCCTATTATGATCAGA
CTGGTGCCTTAGTGGTTGGCCCTGGAGCAAGGACTGGCCTTGGAGCTCCAGTTCGGTTAATGGCTCCAAC
ACCTGTTTTAATTAGTTCAGCAGCAGCACAAGCTGCAGCAGCAGCAGCAGCTGGAGGAACTGCAAGTAGC
CTTACAGGCAGCACAAATGGTCTGTTTCGGCCAATTGGCACTCAGCCACCACAGCAGCAGCAACAGCAGC
CAAGCACTAATCTGCAATCTAATTCATTTTATGGAAGCAGTTCTTTGACTAATAGCTCCCAGAGTAGTTC
TTTATTTTCTCATGGACCTGGTCAACCTGGAAGTACATCTCTTGGCTTTGGAAGTGGTAACTCTTTGGGT
GCTGCTATAGGCTCAGCCCTCAGTGGATTTGGTTCATCAGTTGGCAGTTCTGCAAGTAGTAGTGCCACAA
GGAGAGAGTCTCTATCTACTAGCTCTGACTTGTACAAAAGATCTAGTAGCAGCCTAGCACCCATAGGGCA
ACCATTTTACAATAGTCTGGGATTTTCCTCCTCTCCAAGTCCAATAGGCATGCCTCTGCCAAGCCAAACT
CCAGGACATTCACTTACGCCACCGCCATCACTTTCATCACATGGATCCTCATCCAGTTTGCATTTAGGAG
GACTGACAAATGGTAGTGGTCGATATATCTCTGCAGCACCTGGAGCAGAAGCAAAATATCGAAGTGCTTC
AAGCACTTCCAGTCTATTTAGCTCCAGCAGCCAGCTCTTTCCTCCTTCCCGGCTTCGGTATAATAGGTCT
GATATTATGCCTTCTGGCCGCAGTAGATTATTGGAAGATTTCAGAAACAACCGCTTCCCAAACCTTCAGC
TTAGAGACTTGATTGGACATATAGTTGAGTTTTCTCAAGACCAGCATGGTTCTAGATTCATACAGCAAAA
ACTAGAGAGAGCTACTCCAGCTGAGCGACAGATGGTATTTAATGAAATTCTGCAAGCAGCCTATCAATTA
ATGACTGATGTTTTTGGCAACTATGTTATACAGAAGTTTTTTGAGTTTGGGAGTCTGGATCAAAAATTAG
CCCTGGCTACTCGTATTCGTGGTCATGTTCTACCCTTAGCCTTGCAGATGTATGGCTGCCGCGTTATTCA
GAAAGCATTAGAATCTATTTCTTCTGACCAGCAGAGTGAAATGGTAAAGGAGCTGGATGGTCATGTGCTC
AAATGTGTGAAAGATCAGAATGGAAACCATGTTGTACAAAAATGTATCGAATGTGTTCAGCCACAGTCAC
TACAGTTCATCATTGATGCTTTCAAGGGACAAGTATTTGTGCTTTCAACTCATCCTTATGGCTGCAGAGT
AATTCAGCGCATCCTAGAGCATTGCACTGCAGAACAGACCTTACCTATCTTAGAAGAACTCCACCAACAT
ACAGAGCAGTTGGTACAGGATCAGTATGGCAATTATGTTATTCAGCATGTACTGGAACACGGTCGACCTG
AAGACAAGAGCAAAATTGTTTCCGAAATCAGGGGAAAGGTTTTAGCCCTGAGTCAACACAAATTTGCCAG
CAATGTAGTAGAAAAGTGTGTTACTCATGCCTCCCGTGCTGAGAGAGCTTTACTGATTGACGAGGTTTGC
TGCCAGAATGATGGTCCTCACAGTGCCTTATACACCATGATGAAGGACCAGTATGCCAATTACGTGGTTC
AAAAGATGATTGATATGGCTGAACCTGCTCAGAGAAAGATAATCATGCACAAGATTCGACCTCACATTAC
TACTTTGCGCAAATACACATACGGGAAGCATATACTGGCCAAGTTGGAAAAGTATTATTTGAAGAATAGC
CCGGACCTAGGACCTATTGGAGGACCACCAAATGGAATGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211307 representing NM_015317
Red=Cloning site Green=Tags(s)

MNHDFQALALESRGMGELLPTKKFWEPDDSTKDGQKGIFLGDDEWRETAWGASHHSMSQPIMVQRRSGQG
FHGNSEVNAILSPRSESGGLGVSMVEYVLSSSPADKLDSRFRKGNFGTRDAETDGPEKGDQKGKASPFEE
DQNRDLKQGDDDDSKINGRGLPNGMDADCKDFNRTPGSRQASPTEVVERLGPNTNPSEGLGPLPNPTANK
PLVEEFSNPETQNLDAMEQVGLESLQFDYPGNQVPMDSSGATVGLFDYNSQQQLFQRTNALTVQQLTAAQ
QQQYALAAAQQPHIAGVFSAGLAPAAFVPNPYIISAAPPGTDPYTAAGLAAAATLAGPAVVPPQYYGVPW
GVYPANLFQQQAAAAANNTASQQAASQAQPGQQQVLRAGAGQRPLTPNQGQQGQQAESLAAAAAANPTLA
FGQGLATGMPGYQVLAPTAYYDQTGALVVGPGARTGLGAPVRLMAPTPVLISSAAAQAAAAAAAGGTASS
LTGSTNGLFRPIGTQPPQQQQQQPSTNLQSNSFYGSSSLTNSSQSSSLFSHGPGQPGSTSLGFGSGNSLG
AAIGSALSGFGSSVGSSASSSATRRESLSTSSDLYKRSSSSLAPIGQPFYNSLGFSSSPSPIGMPLPSQT
PGHSLTPPPSLSSHGSSSSLHLGGLTNGSGRYISAAPGAEAKYRSASSTSSLFSSSSQLFPPSRLRYNRS
DIMPSGRSRLLEDFRNNRFPNLQLRDLIGHIVEFSQDQHGSRFIQQKLERATPAERQMVFNEILQAAYQL
MTDVFGNYVIQKFFEFGSLDQKLALATRIRGHVLPLALQMYGCRVIQKALESISSDQQSEMVKELDGHVL
KCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKGQVFVLSTHPYGCRVIQRILEHCTAEQTLPILEELHQH
TEQLVQDQYGNYVIQHVLEHGRPEDKSKIVSEIRGKVLALSQHKFASNVVEKCVTHASRAERALLIDEVC
CQNDGPHSALYTMMKDQYANYVVQKMIDMAEPAQRKIIMHKIRPHITTLRKYTYGKHILAKLEKYYLKNS
PDLGPIGGPPNGML

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_015317
ORF Size 3192 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015317.5
RefSeq Size 6111 bp
RefSeq ORF 3195 bp
Locus ID 23369
UniProt ID Q8TB72
Cytogenetics 2p24.1
Domains PUF
MW 113.8 kDa
Gene Summary This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

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