LI Cadherin (CDH17) (NM_004063) Human Tagged ORF Clone

CAT#: RC211298

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CDH17 (Myc-DDK-tagged)-Human cadherin 17, LI cadherin (liver-intestine) (CDH17), transcript variant 1



  "NM_004063" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 762.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CDH17
Synonyms CDH16; HPT-1; HPT1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211298 representing NM_004063
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATACTTCAGGCCCATCTTCACTCCCTGTGTCTTCTTATGCTTTATTTGGCAACTGGATATGGCCAAG
AGGGGAAGTTTAGTGGACCCCTGAAACCCATGACATTTTCTATTTATGAAGGCCAAGAACCGAGTCAAAT
TATATTCCAGTTTAAGGCCAATCCTCCTGCTGTGACTTTTGAACTAACTGGGGAGACAGACAACATATTT
GTGATAGAACGGGAGGGACTTCTGTATTACAACAGAGCCTTGGACAGGGAAACAAGATCTACTCACAATC
TCCAGGTTGCAGCCCTGGACGCTAATGGAATTATAGTGGAGGGTCCAGTCCCTATCACCATAAAAGTGAA
GGACATCAACGACAATCGACCCACGTTTCTCCAGTCAAAGTACGAAGGCTCAGTAAGGCAGAACTCTCGC
CCAGGAAAGCCCTTCTTGTATGTCAATGCCACAGACCTGGATGATCCGGCCACTCCCAATGGCCAGCTTT
ATTACCAGATTGTCATCCAGCTTCCCATGATCAACAATGTCATGTACTTTCAGATCAACAACAAAACGGG
AGCCATCTCTCTTACCCGAGAGGGATCTCAGGAATTGAATCCTGCTAAGAATCCTTCCTATAATCTGGTG
ATCTCAGTGAAGGACATGGGAGGCCAGAGTGAGAATTCCTTCAGTGATACCACATCTGTGGATATCATAG
TGACAGAGAATATTTGGAAAGCACCAAAACCTGTGGAGATGGTGGAAAACTCAACTGATCCTCACCCCAT
CAAAATCACTCAGGTGCGGTGGAATGATCCCGGTGCACAATATTCCTTAGTTGACAAAGAGAAGCTGCCA
AGATTCCCATTTTCAATTGACCAGGAAGGAGATATTTACGTGACTCAGCCCTTGGACCGAGAAGAAAAGG
ATGCATATGTTTTTTATGCAGTTGCAAAGGATGAGTACGGAAAACCACTTTCATATCCGCTGGAAATTCA
TGTAAAAGTTAAAGATATTAATGATAATCCACCTACATGTCCGTCACCAGTAACCGTATTTGAGGTCCAG
GAGAATGAACGACTGGGTAACAGTATCGGGACCCTTACTGCACATGACAGGGATGAAGAAAATACTGCCA
ACAGTTTTCTAAACTACAGGATTGTGGAGCAAACTCCCAAACTTCCCATGGATGGACTCTTCCTAATCCA
AACCTATGCTGGAATGTTACAGTTAGCTAAACAGTCCTTGAAGAAGCAAGATACTCCTCAGTACAACTTA
ACGATAGAGGTGTCTGACAAAGATTTCAAGACCCTTTGTTTTGTGCAAATCAACGTTATTGATATCAATG
ATCAGATCCCCATCTTTGAAAAATCAGATTATGGAAACCTGACTCTTGCTGAAGACACAAACATTGGGTC
CACCATCTTAACCATCCAGGCCACTGATGCTGATGAGCCATTTACTGGGAGTTCTAAAATTCTGTATCAT
ATCATAAAGGGAGACAGTGAGGGACGCCTGGGGGTTGACACAGATCCCCATACCAACACCGGATATGTCA
TAATTAAAAAGCCTCTTGATTTTGAAACAGCAGCTGTTTCCAACATTGTGTTCAAAGCAGAAAATCCTGA
GCCTCTAGTGTTTGGTGTGAAGTACAATGCAAGTTCTTTTGCCAAGTTCACGCTTATTGTGACAGATGTG
AATGAAGCACCTCAATTTTCCCAACACGTATTCCAAGCGAAAGTCAGTGAGGATGTAGCTATAGGCACTA
AAGTGGGCAATGTGACTGCCAAGGATCCAGAAGGTCTGGACATAAGCTATTCACTGAGGGGAGACACAAG
AGGTTGGCTTAAAATTGACCACGTGACTGGTGAGATCTTTAGTGTGGCTCCATTGGACAGAGAAGCCGGA
AGTCCATATCGGGTACAAGTGGTGGCCACAGAAGTAGGGGGGTCTTCCTTAAGCTCTGTGTCAGAGTTCC
ACCTGATCCTTATGGATGTGAATGACAACCCTCCCAGGCTAGCCAAGGACTACACGGGCTTGTTCTTCTG
CCATCCCCTCAGTGCACCTGGAAGTCTCATTTTCGAGGCTACTGATGATGATCAGCACTTATTTCGGGGT
CCCCATTTTACATTTTCCCTCGGCAGTGGAAGCTTACAAAACGACTGGGAAGTTTCCAAAATCAATGGTA
CTCATGCCCGACTGTCTACCAGGCACACAGACTTTGAGGAGAGGGCGTATGTCGTCTTGATCCGCATCAA
TGATGGGGGTCGGCCACCCTTGGAAGGCATTGTTTCTTTACCAGTTACATTCTGCAGTTGTGTGGAAGGA
AGTTGTTTCCGGCCAGCAGGTCACCAGACTGGGATACCCACTGTGGGCATGGCAGTTGGTATACTGCTGA
CCACCCTTCTGGTGATTGGTATAATTTTAGCAGTTGTGTTTATCCGCATAAAGAAGGATAAAGGCAAAGA
TAATGTTGAAAGTGCTCAAGCATCTGAAGTCAAACCTCTGAGAAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211298 representing NM_004063
Red=Cloning site Green=Tags(s)

MILQAHLHSLCLLMLYLATGYGQEGKFSGPLKPMTFSIYEGQEPSQIIFQFKANPPAVTFELTGETDNIF
VIEREGLLYYNRALDRETRSTHNLQVAALDANGIIVEGPVPITIKVKDINDNRPTFLQSKYEGSVRQNSR
PGKPFLYVNATDLDDPATPNGQLYYQIVIQLPMINNVMYFQINNKTGAISLTREGSQELNPAKNPSYNLV
ISVKDMGGQSENSFSDTTSVDIIVTENIWKAPKPVEMVENSTDPHPIKITQVRWNDPGAQYSLVDKEKLP
RFPFSIDQEGDIYVTQPLDREEKDAYVFYAVAKDEYGKPLSYPLEIHVKVKDINDNPPTCPSPVTVFEVQ
ENERLGNSIGTLTAHDRDEENTANSFLNYRIVEQTPKLPMDGLFLIQTYAGMLQLAKQSLKKQDTPQYNL
TIEVSDKDFKTLCFVQINVIDINDQIPIFEKSDYGNLTLAEDTNIGSTILTIQATDADEPFTGSSKILYH
IIKGDSEGRLGVDTDPHTNTGYVIIKKPLDFETAAVSNIVFKAENPEPLVFGVKYNASSFAKFTLIVTDV
NEAPQFSQHVFQAKVSEDVAIGTKVGNVTAKDPEGLDISYSLRGDTRGWLKIDHVTGEIFSVAPLDREAG
SPYRVQVVATEVGGSSLSSVSEFHLILMDVNDNPPRLAKDYTGLFFCHPLSAPGSLIFEATDDDQHLFRG
PHFTFSLGSGSLQNDWEVSKINGTHARLSTRHTDFEERAYVVLIRINDGGRPPLEGIVSLPVTFCSCVEG
SCFRPAGHQTGIPTVGMAVGILLTTLLVIGIILAVVFIRIKKDKGKDNVESAQASEVKPLRS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004063
ORF Size 2496 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004063.4
RefSeq Size 3697 bp
RefSeq ORF 2499 bp
Locus ID 1015
UniProt ID Q12864
Cytogenetics 8q22.1
Protein Families Transmembrane
MW 92.15 kDa
Gene Summary This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]

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