ODF4 (NM_153007) Human Tagged ORF Clone

CAT#: RC211295

ODF4 (Myc-DDK-tagged)-Human outer dense fiber of sperm tails 4 (ODF4)

Product Images

Circular map for RC211295

• 

  Western blot validation of overexpression lysate (Cat# LY407193) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211295 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ODF4 protein (Cat# TP311295). The protein was produced from HEK293T cells transfected with ODF4 cDNA clone (Cat# RC211295) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ODF4
• Synonyms: CT134; CT136; OPPO1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211295 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCAGAGTACTCTGGGAATGAGTTCCCCAGGTCAGAAGGAGAAAGAGACCAACATCAGAGACCTG
  GAAAGGAAAGGAAGAGTGGGGAGGCAGGACGGGGCACAGGTGAGCTGGGACAAGATGGGAGACTGCTGTC
  CTCCACCCTCTCCCTCAGTAGTAACAGGTCCTTGGGCCAGCGCCAGAACTCTCCGCTGCCCTTTCAATGG
  AGAATCACACACAGCTTCCGCTGGATGGCCCAGGTGTTGGCCTCTGAGCTCAGCCTGGTTGCCTTTATCC
  TACTATTGGTCATGGCCTTCTCCAAGAAATGGCTGGACCTCTCTAGGAGCCTCTTCTACCAGCGCTGGCC
  CGTGGATGTCAGCAACAGAATCCACACATCAGCCCACGTTATGTCCATGGGGCTCCTGCACTTTTGCAAA
  TCCAGGAGCTGTTCTGACTTAGAGAATGGGAAAGTCACCTTCATCTTCTCCACCCTCATGCTATTCCCCA
  TTAACATCTGGATCTTCGAGTTGGAAAGGAATGTATCCATCCCCATAGGCTGGAGCTATTTCATTGGTTG
  GCTGGTGCTTATCCTATACTTCACCTGCGCGATCCTTTGCTACTTCAACCATAAAAGTTTCTGGAGTCTG
  ATTCTGAGCCACCCCAGTGGTGCCGTGTCCTGCAGCAGCAGTTTCGGCTCAGTAGAAGAATCTCCAAGGG
  CACAGACGATCACAGACACCCCCATCACCCAGGAGGGAGTCCTGGATCCTGAGCAGAAGGATACACATGT
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211295 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDAEYSGNEFPRSEGERDQHQRPGKERKSGEAGRGTGELGQDGRLLSSTLSLSSNRSLGQRQNSPLPFQW
  RITHSFRWMAQVLASELSLVAFILLLVMAFSKKWLDLSRSLFYQRWPVDVSNRIHTSAHVMSMGLLHFCK
  SRSCSDLENGKVTFIFSTLMLFPINIWIFELERNVSIPIGWSYFIGWLVLILYFTCAILCYFNHKSFWSL
  ILSHPSGAVSCSSSFGSVEESPRAQTITDTPITQEGVLDPEQKDTHV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153007
• ORF Size: 771 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153007.5
• RefSeq Size: 1149 bp
• RefSeq ORF: 774 bp
• Locus ID: 146852
• UniProt ID: Q2M2E3
• Cytogenetics: 17p13.1
• Protein Families: Transmembrane
• MW: 29.2 kDa
• Gene Summary: This gene encodes a protein that is localized in the outer dense fibers of the tails of mature sperm. This protein is thought to have some important role in the sperm tail. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]