TRPV3 (NM_145068) Human Tagged ORF Clone

CAT#: RC211184

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TRPV3 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily V, member 3 (TRPV3)



  "NM_145068" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


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In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TRPV3
Synonyms FNEPPK2; OLMS; OLMS1; VRL3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211184 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGCCCACCCCAAGGAGATGGTGCCTCTCATGGGCAAGAGAGTTGCTGCCCCCAGTGGGAACCCTG
CCGTCCTGCCAGAGAAGAGGCCGGCGGAGATCACCCCCACAAAGAAGAGTGCACACTTCTTCCTGGAGAT
AGAAGGGTTTGAACCCAACCCCACAGTTGCCAAGACCTCTCCTCCTGTCTTCTCCAAGCCCATGGATTCC
AACATCCGGCAGTGCATCTCTGGTAACTGTGATGACATGGACTCCCCCCAGTCTCCTCAAGATGATGTGA
CAGAGACCCCATCCAATCCCAACAGCCCCAGTGCACAGCTGGCCAAGGAAGAGCAGAGGAGGAAAAAGGG
GCGGCTGAAGAAGCGCATCTTTGCAGCCGTGTCTGAGGGCTGCGTGGAGGAGTTGGTAGAGTTGCTGGTG
GAGCTGCAGGAGCTTTGCAGGCGGCGCCATGATGAGGATGTGCCTGACTTCCTCATGCACAAGCTGACGG
CCTCCGACACGGGGAAGACCTGCCTGATGAAGGCCTTGTTAAACATCAACCCCAACACCAAGGAGATCGT
GCGGATCCTGCTTGCCTTTGCTGAAGAGAACAACATCCTGGGCAGGTTCATCAACGCCGAGTACACAGAG
GAGGCCTATGAAGGGCAGACGGCGCTGAACATCGCCATCGAGCGGCGGCAGGGGGACATCGCAGCCCTGC
TCATCGCCGCCGGCGCCGACGTCAACGCGCACGCCAAGGGGGCCTTCTTCAACCCCAAGTACCAACACGA
AGGCTTCTACTTCGGTGAGACGCCCCTGGCCCTGGCAGCATGCACCAACCAGCCCGAGATTGTGCAGCTG
CTGATGGAGCACGAGCAGACGGACATCACCTCGCGGGACTCACGAGGCAACAACATCCTTCACGCCCTGG
TGACCGTGGCCGAGGACTTCAAGACGCAGAATGACTTTGTGAAGCGCATGTACGACATGATCCTACTGCG
GAGTGGCAACTGGGAGCTGGAGACCACTCGCAACAACGATGGCCTCACGCCGCTGCAGCTGGCCGCCAAG
ATGGGCAAGGCGGAGATCCTGAAGTACATCCTCAGTCGTGAGATCAAGGAGAAGCGGCTCCGGAGCCTGT
CCAGGAAGTTCACCGACTGGGCGTACGGACCCGTGTCATCCTCCCTCTACGACCTCACCAACGTGGACAC
CACCACGGACAACTCAGTGCTGGAAATCACTGTCTACAACACCAACATCGACAACCGGCATGAGATGCTG
ACCCTGGAGCCGCTGCACACGCTGCTGCATATGAAGTGGAAGAAGTTTGCCAAGCACATGTTCTTTCTGT
CCTTCTGCTTTTATTTCTTCTACAACATCACCCTGACCCTCGTCTCGTACTACCGCCCCCGGGAGGAGGA
GGCCATCCCGCACCCCTTGGCCCTGACGCACAAGATGGGGTGGCTGCAGCTCCTAGGGAGGATGTTTGTG
CTCATCTGGGCCATGTGCATCTCTGTGAAAGAGGGCATTGCCATCTTCCTGCTGAGACCCTCGGATCTGC
AGTCCATCCTCTCGGATGCCTGGTTCCACTTTGTCTTTTTTATCCAAGCTGTGCTTGTGATACTGTCTGT
CTTCTTGTACTTGTTTGCCTACAAAGAGTACCTCGCCTGCCTCGTGCTGGCCATGGCCCTGGGCTGGGCG
AACATGCTCTACTATACGCGGGGTTTCCAGTCCATGGGCATGTACAGCGTCATGATCCAGAAGGTCATTT
TGCATGATGTTCTGAAGTTCTTGTTTGTATATATCGTGTTTTTGCTTGGATTTGGAGTAGCCTTGGCCTC
GCTGATCGAGAAGTGTCCCAAAGACAACAAGGACTGCAGCTCCTACGGCAGCTTCAGCGACGCAGTGCTG
GAACTCTTCAAGCTCACCATAGGCCTGGGTGATCTGAACATCCAGCAGAACTCCAAGTATCCCATTCTCT
TTCTGTTCCTGCTCATCACCTATGTCATCCTCACCTTTGTTCTCCTCCTCAACATGCTCATTGCTCTGAT
GGGCGAGACTGTGGAGAACGTCTCCAAGGAGAGCGAACGCATCTGGCGCCTGCAGAGAGCCAGGACCATC
TTGGAGTTTGAGAAAATGTTACCAGAATGGCTGAGGAGCAGATTCCGGATGGGAGAGCTGTGCAAAGTGG
CCGAGGATGATTTCCGACTGTGTTTGCGGATCAATGAGGTGAAGTGGACTGAATGGAAGACGCACGTCTC
CTTCCTTAACGAAGACCCGGGGCCTGTAAGACGAACAGCAGATTTCAACAAAATCCAAGATTCTTCCAGG
AACAACAGCAAAACCACTCTCAATGCATTTGAAGAAGTCGAGGAATTCCCGGAAACCTCGGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211184 protein sequence
Red=Cloning site Green=Tags(s)

MKAHPKEMVPLMGKRVAAPSGNPAVLPEKRPAEITPTKKSAHFFLEIEGFEPNPTVAKTSPPVFSKPMDS
NIRQCISGNCDDMDSPQSPQDDVTETPSNPNSPSAQLAKEEQRRKKGRLKKRIFAAVSEGCVEELVELLV
ELQELCRRRHDEDVPDFLMHKLTASDTGKTCLMKALLNINPNTKEIVRILLAFAEENNILGRFINAEYTE
EAYEGQTALNIAIERRQGDIAALLIAAGADVNAHAKGAFFNPKYQHEGFYFGETPLALAACTNQPEIVQL
LMEHEQTDITSRDSRGNNILHALVTVAEDFKTQNDFVKRMYDMILLRSGNWELETTRNNDGLTPLQLAAK
MGKAEILKYILSREIKEKRLRSLSRKFTDWAYGPVSSSLYDLTNVDTTTDNSVLEITVYNTNIDNRHEML
TLEPLHTLLHMKWKKFAKHMFFLSFCFYFFYNITLTLVSYYRPREEEAIPHPLALTHKMGWLQLLGRMFV
LIWAMCISVKEGIAIFLLRPSDLQSILSDAWFHFVFFIQAVLVILSVFLYLFAYKEYLACLVLAMALGWA
NMLYYTRGFQSMGMYSVMIQKVILHDVLKFLFVYIVFLLGFGVALASLIEKCPKDNKDCSSYGSFSDAVL
ELFKLTIGLGDLNIQQNSKYPILFLFLLITYVILTFVLLLNMLIALMGETVENVSKESERIWRLQRARTI
LEFEKMLPEWLRSRFRMGELCKVAEDDFRLCLRINEVKWTEWKTHVSFLNEDPGPVRRTADFNKIQDSSR
NNSKTTLNAFEEVEEFPETSV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_145068
ORF Size 2373 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_145068.4
RefSeq Size 6130 bp
RefSeq ORF 2373 bp
Locus ID 162514
UniProt ID Q8NET8
Cytogenetics 17p13.2
Protein Families Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
MW 90.6 kDa
Gene Summary This gene product belongs to a family of nonselective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermosensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. This channel is activated at temperatures between 22 and 40 degrees C. This gene lies in close proximity to another family member gene on chromosome 17, and the two encoded proteins are thought to associate with each other to form heteromeric channels. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

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