TRPV3 (NM_145068) Human Tagged ORF Clone

CAT#: RC211184

TRPV3 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily V, member 3 (TRPV3)

Product Images

Western blot validation of overexpression lysate (Cat# LY403422) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211184 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRPV3
• Synonyms: FNEPPK2; OLMS; OLMS1; VRL3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211184 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGCCCACCCCAAGGAGATGGTGCCTCTCATGGGCAAGAGAGTTGCTGCCCCCAGTGGGAACCCTG
  CCGTCCTGCCAGAGAAGAGGCCGGCGGAGATCACCCCCACAAAGAAGAGTGCACACTTCTTCCTGGAGAT
  AGAAGGGTTTGAACCCAACCCCACAGTTGCCAAGACCTCTCCTCCTGTCTTCTCCAAGCCCATGGATTCC
  AACATCCGGCAGTGCATCTCTGGTAACTGTGATGACATGGACTCCCCCCAGTCTCCTCAAGATGATGTGA
  CAGAGACCCCATCCAATCCCAACAGCCCCAGTGCACAGCTGGCCAAGGAAGAGCAGAGGAGGAAAAAGGG
  GCGGCTGAAGAAGCGCATCTTTGCAGCCGTGTCTGAGGGCTGCGTGGAGGAGTTGGTAGAGTTGCTGGTG
  GAGCTGCAGGAGCTTTGCAGGCGGCGCCATGATGAGGATGTGCCTGACTTCCTCATGCACAAGCTGACGG
  CCTCCGACACGGGGAAGACCTGCCTGATGAAGGCCTTGTTAAACATCAACCCCAACACCAAGGAGATCGT
  GCGGATCCTGCTTGCCTTTGCTGAAGAGAACAACATCCTGGGCAGGTTCATCAACGCCGAGTACACAGAG
  GAGGCCTATGAAGGGCAGACGGCGCTGAACATCGCCATCGAGCGGCGGCAGGGGGACATCGCAGCCCTGC
  TCATCGCCGCCGGCGCCGACGTCAACGCGCACGCCAAGGGGGCCTTCTTCAACCCCAAGTACCAACACGA
  AGGCTTCTACTTCGGTGAGACGCCCCTGGCCCTGGCAGCATGCACCAACCAGCCCGAGATTGTGCAGCTG
  CTGATGGAGCACGAGCAGACGGACATCACCTCGCGGGACTCACGAGGCAACAACATCCTTCACGCCCTGG
  TGACCGTGGCCGAGGACTTCAAGACGCAGAATGACTTTGTGAAGCGCATGTACGACATGATCCTACTGCG
  GAGTGGCAACTGGGAGCTGGAGACCACTCGCAACAACGATGGCCTCACGCCGCTGCAGCTGGCCGCCAAG
  ATGGGCAAGGCGGAGATCCTGAAGTACATCCTCAGTCGTGAGATCAAGGAGAAGCGGCTCCGGAGCCTGT
  CCAGGAAGTTCACCGACTGGGCGTACGGACCCGTGTCATCCTCCCTCTACGACCTCACCAACGTGGACAC
  CACCACGGACAACTCAGTGCTGGAAATCACTGTCTACAACACCAACATCGACAACCGGCATGAGATGCTG
  ACCCTGGAGCCGCTGCACACGCTGCTGCATATGAAGTGGAAGAAGTTTGCCAAGCACATGTTCTTTCTGT
  CCTTCTGCTTTTATTTCTTCTACAACATCACCCTGACCCTCGTCTCGTACTACCGCCCCCGGGAGGAGGA
  GGCCATCCCGCACCCCTTGGCCCTGACGCACAAGATGGGGTGGCTGCAGCTCCTAGGGAGGATGTTTGTG
  CTCATCTGGGCCATGTGCATCTCTGTGAAAGAGGGCATTGCCATCTTCCTGCTGAGACCCTCGGATCTGC
  AGTCCATCCTCTCGGATGCCTGGTTCCACTTTGTCTTTTTTATCCAAGCTGTGCTTGTGATACTGTCTGT
  CTTCTTGTACTTGTTTGCCTACAAAGAGTACCTCGCCTGCCTCGTGCTGGCCATGGCCCTGGGCTGGGCG
  AACATGCTCTACTATACGCGGGGTTTCCAGTCCATGGGCATGTACAGCGTCATGATCCAGAAGGTCATTT
  TGCATGATGTTCTGAAGTTCTTGTTTGTATATATCGTGTTTTTGCTTGGATTTGGAGTAGCCTTGGCCTC
  GCTGATCGAGAAGTGTCCCAAAGACAACAAGGACTGCAGCTCCTACGGCAGCTTCAGCGACGCAGTGCTG
  GAACTCTTCAAGCTCACCATAGGCCTGGGTGATCTGAACATCCAGCAGAACTCCAAGTATCCCATTCTCT
  TTCTGTTCCTGCTCATCACCTATGTCATCCTCACCTTTGTTCTCCTCCTCAACATGCTCATTGCTCTGAT
  GGGCGAGACTGTGGAGAACGTCTCCAAGGAGAGCGAACGCATCTGGCGCCTGCAGAGAGCCAGGACCATC
  TTGGAGTTTGAGAAAATGTTACCAGAATGGCTGAGGAGCAGATTCCGGATGGGAGAGCTGTGCAAAGTGG
  CCGAGGATGATTTCCGACTGTGTTTGCGGATCAATGAGGTGAAGTGGACTGAATGGAAGACGCACGTCTC
  CTTCCTTAACGAAGACCCGGGGCCTGTAAGACGAACAGCAGATTTCAACAAAATCCAAGATTCTTCCAGG
  AACAACAGCAAAACCACTCTCAATGCATTTGAAGAAGTCGAGGAATTCCCGGAAACCTCGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211184 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKAHPKEMVPLMGKRVAAPSGNPAVLPEKRPAEITPTKKSAHFFLEIEGFEPNPTVAKTSPPVFSKPMDS
  NIRQCISGNCDDMDSPQSPQDDVTETPSNPNSPSAQLAKEEQRRKKGRLKKRIFAAVSEGCVEELVELLV
  ELQELCRRRHDEDVPDFLMHKLTASDTGKTCLMKALLNINPNTKEIVRILLAFAEENNILGRFINAEYTE
  EAYEGQTALNIAIERRQGDIAALLIAAGADVNAHAKGAFFNPKYQHEGFYFGETPLALAACTNQPEIVQL
  LMEHEQTDITSRDSRGNNILHALVTVAEDFKTQNDFVKRMYDMILLRSGNWELETTRNNDGLTPLQLAAK
  MGKAEILKYILSREIKEKRLRSLSRKFTDWAYGPVSSSLYDLTNVDTTTDNSVLEITVYNTNIDNRHEML
  TLEPLHTLLHMKWKKFAKHMFFLSFCFYFFYNITLTLVSYYRPREEEAIPHPLALTHKMGWLQLLGRMFV
  LIWAMCISVKEGIAIFLLRPSDLQSILSDAWFHFVFFIQAVLVILSVFLYLFAYKEYLACLVLAMALGWA
  NMLYYTRGFQSMGMYSVMIQKVILHDVLKFLFVYIVFLLGFGVALASLIEKCPKDNKDCSSYGSFSDAVL
  ELFKLTIGLGDLNIQQNSKYPILFLFLLITYVILTFVLLLNMLIALMGETVENVSKESERIWRLQRARTI
  LEFEKMLPEWLRSRFRMGELCKVAEDDFRLCLRINEVKWTEWKTHVSFLNEDPGPVRRTADFNKIQDSSR
  NNSKTTLNAFEEVEEFPETSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145068
• ORF Size: 2373 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145068.4
• RefSeq Size: 6130 bp
• RefSeq ORF: 2373 bp
• Locus ID: 162514
• UniProt ID: Q8NET8
• Cytogenetics: 17p13.2
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• MW: 90.6 kDa
• Gene Summary: This gene product belongs to a family of nonselective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermosensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. This channel is activated at temperatures between 22 and 40 degrees C. This gene lies in close proximity to another family member gene on chromosome 17, and the two encoded proteins are thought to associate with each other to form heteromeric channels. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]