KDM4C (NM_015061) Human Tagged ORF Clone

CAT#: RC211182

KDM4C (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 4C (KDM4C), transcript variant 1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY KDM4C (Cat# RC211182, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KDM4C(Cat# TA500567). Positive lysates LY402405 (100ug) and LC402405 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY402405) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211182 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KDM4C protein (Cat# TP311182). The protein was produced from HEK293T cells transfected with KDM4C cDNA clone (Cat# RC211182) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KDM4C
• Synonyms: GASC1; JHDM3C; JMJD2C; TDRD14C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211182 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTGGCCGAGGTGGAAAGTCCTCTGAACCCCAGCTGTAAGATAATGACCTTCAGACCCTCCATGG
  AGGAGTTCCGGGAGTTCAACAAATACCTTGCATACATGGAGTCTAAAGGAGCCCATCGTGCGGGTCTTGC
  AAAGGTGATTCCTCCTAAGGAGTGGAAGCCAAGACAGTGCTATGATGACATTGATAATTTGCTCATTCCA
  GCACCAATTCAGCAGATGGTCACAGGGCAGTCAGGACTGTTCACTCAGTACAACATCCAGAAAAAAGCGA
  TGACTGTGAAGGAGTTCAGGCAGCTGGCCAACAGTGGCAAATATTGTACTCCAAGATACTTGGATTACGA
  AGATTTGGAGCGCAAGTACTGGAAGAACTTAACTTTTGTGGCACCTATCTATGGTGCAGATATTAATGGG
  AGCATATATGATGAGGGTGTGGATGAATGGAACATAGCTCGCCTCAATACAGTCTTGGATGTGGTTGAAG
  AAGAGTGTGGCATTTCTATTGAGGGTGTAAATACCCCATATCTCTATTTTGGCATGTGGAAGACCACGTT
  TGCATGGCACACCGAAGACATGGACCTCTATAGCATTAATTATCTCCACTTTGGAGAGCCCAAGTCTTGG
  TATGCTATACCTCCGGAGCATGGAAAACGACTTGAAAGACTAGCTCAAGGTTTTTTCCCAAGCAGCTCCC
  AAGGGTGTGATGCATTTCTTCGCCACAAGATGACATTGATTTCTCCATCAGTATTGAAGAAATATGGTAT
  TCCCTTTGACAAGATAACCCAGGAGGCTGGAGAATTCATGATCACTTTCCCATATGGCTACCATGCTGGT
  TTTAATCATGGTTTCAACTGTGCAGAATCTACAAATTTTGCTACTGTCAGATGGATTGACTATGGAAAAG
  TTGCCAAATTGTGCACTTGCAGGAAAGACATGGTGAAGATTTCAATGGATATCTTTGTGAGGAAATTTCA
  GCCAGACAGATATCAGCTTTGGAAACAAGGAAAGGATATATACACCATTGATCACACGAAGCCTACTCCA
  GCATCCACCCCTGAAGTAAAAGCATGGCTGCAGAGGAGGAGGAAAGTAAGAAAAGCATCCCGAAGCTTCC
  AGTGTGCTAGGTCTACCTCTAAAAGGCCTAAGGCTGATGAGGAAGAGGAAGTGTCAGATGAAGTCGATGG
  GGCAGAGGTCCCTAACCCCGACTCAGTCACAGATGACCTCAAGGTCAGTGAAAAGTCAGAAGCAGCAGTG
  AAGCTGAGGAACACAGAAGCATCTTCAGAAGAAGAGTCATCTGCTAGCAGGATGCAGGTGGAGCAGAATT
  TATCAGATCATATCAAACTCTCAGGAAACAGCTGCTTAAGTACATCTGTAACAGAAGACATAAAAACTGA
  GGATGACAAAGCTTATGCATATAGAAGTGTACCTTCTATATCCAGTGAGGCTGATGATTCCATTCCATTG
  TCTAGTGGCTATGAGAAGCCCGAGAAATCAGACCCATCCGAGCTTTCATGGCCAAAGTCACCTGAGTCAT
  GCTCATCAGTGGCAGAGAGTAATGGTGTGTTAACAGAGGGAGAAGAGAGTGATGTGGAGAGCCATGGGAA
  TGGCCTTGAACCTGGGGAAATCCCAGCGGTCCCCAGTGGAGAGAGAAATAGCTTCAAAGTCCCCAGTATA
  GCAGAGGGAGAGAACAAAACCTCTAAGAGTTGGCGCCATCCACTTAGCAGGCCTCCAGCAAGATCTCCGA
  TGACTCTTGTGAAGCAGCAGGCGCCAAGTGATGAAGAATTGCCTGAGGTTCTGTCCATTGAGGAGGAAGT
  GGAAGAAACAGAGTCTTGGGCGAAACCTCTCATCCACCTTTGGCAGACGAAGTCCCCTAACTTCGCAGCT
  GAGCAAGAGTATAATGCAACAGTGGCCAGGATGAAGCCACACTGTGCCATCTGCACTCTGCTCATGCCGT
  ACCACAAGCCAGATAGCAGCAATGAAGAAAATGATGCTAGATGGGAGACAAAATTAGATGAAGTCGTTAC
  ATCGGAGGGAAAGACTAAGCCCCTCATACCAGAGATGTGTTTTATTTATAGTGAAGAAAATATAGAATAT
  TCTCCACCCAATGCCTTCCTTGAAGAGGATGGAACAAGTCTCCTTATTTCCTGTGCAAAGTGCTGCGTAC
  GGGTTCATGCAAGTTGTTATGGTATTCCTTCTCATGAGATCTGTGATGGATGGCTGTGTGCCCGGTGCAA
  AAGAAATGCGTGGACAGCAGAATGCTGTCTCTGCAATTTGAGAGGAGGTGCTCTTAAGCAAACGAAGAAC
  AATAAGTGGGCCCATGTCATGTGCGCCGTTGCGGTCCCAGAAGTTCGATTCACTAATGTCCCAGAAAGGA
  CACAAATAGATGTAGGCAGAATACCTTTACAGAGGTTAAAATTGAAATGCATCTTCTGCAGACACCGGGT
  TAAGAGGGTCTCTGGAGCCTGCATCCAGTGTTCCTACGGTCGCTGCCCGGCCTCCTTCCATGTCACTTGT
  GCCCATGCTGCTGGGGTACTGATGGAGCCTGATGATTGGCCTTATGTGGTGAACATTACATGCTTTCGAC
  ATAAGGTCAACCCCAACGTGAAGTCCAAGGCTTGCGAGAAGGTCATTTCCGTGGGTCAAACGGTCATCAC
  GAAGCATCGGAACACCCGGTATTACAGTTGCAGAGTGATGGCTGTGACATCGCAGACCTTCTATGAGGTC
  ATGTTTGATGATGGCTCCTTTAGCAGAGACACATTTCCTGAGGATATCGTGAGCCGAGACTGTCTGAAGC
  TGGGCCCACCTGCTGAGGGAGAAGTCGTCCAAGTCAAGTGGCCCGATGGCAAACTCTATGGAGCAAAATA
  TTTTGGATCAAATATTGCCCACATGTACCAGGTTGAGTTTGAAGATGGATCCCAGATAGCAATGAAGAGA
  GAGGACATCTACACTTTAGATGAAGAGTTACCCAAGAGAGTGAAAGCTCGATTTTCCACAGCCTCTGACA
  TGCGATTTGAAGACACGTTTTATGGAGCAGACATTATCCAAGGGGAGAGAAAGAGACAAAGAGTGCTGAG
  CTCCAGGTTTAAGAATGAATATGTGGCCGACCCTATATACCGCACTTTTTTGAAGAGCTCTTTCCAGAAG
  AAGTGCCAGAAGAGACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211182 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEVAEVESPLNPSCKIMTFRPSMEEFREFNKYLAYMESKGAHRAGLAKVIPPKEWKPRQCYDDIDNLLIP
  APIQQMVTGQSGLFTQYNIQKKAMTVKEFRQLANSGKYCTPRYLDYEDLERKYWKNLTFVAPIYGADING
  SIYDEGVDEWNIARLNTVLDVVEEECGISIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYLHFGEPKSW
  YAIPPEHGKRLERLAQGFFPSSSQGCDAFLRHKMTLISPSVLKKYGIPFDKITQEAGEFMITFPYGYHAG
  FNHGFNCAESTNFATVRWIDYGKVAKLCTCRKDMVKISMDIFVRKFQPDRYQLWKQGKDIYTIDHTKPTP
  ASTPEVKAWLQRRRKVRKASRSFQCARSTSKRPKADEEEEVSDEVDGAEVPNPDSVTDDLKVSEKSEAAV
  KLRNTEASSEEESSASRMQVEQNLSDHIKLSGNSCLSTSVTEDIKTEDDKAYAYRSVPSISSEADDSIPL
  SSGYEKPEKSDPSELSWPKSPESCSSVAESNGVLTEGEESDVESHGNGLEPGEIPAVPSGERNSFKVPSI
  AEGENKTSKSWRHPLSRPPARSPMTLVKQQAPSDEELPEVLSIEEEVEETESWAKPLIHLWQTKSPNFAA
  EQEYNATVARMKPHCAICTLLMPYHKPDSSNEENDARWETKLDEVVTSEGKTKPLIPEMCFIYSEENIEY
  SPPNAFLEEDGTSLLISCAKCCVRVHASCYGIPSHEICDGWLCARCKRNAWTAECCLCNLRGGALKQTKN
  NKWAHVMCAVAVPEVRFTNVPERTQIDVGRIPLQRLKLKCIFCRHRVKRVSGACIQCSYGRCPASFHVTC
  AHAAGVLMEPDDWPYVVNITCFRHKVNPNVKSKACEKVISVGQTVITKHRNTRYYSCRVMAVTSQTFYEV
  MFDDGSFSRDTFPEDIVSRDCLKLGPPAEGEVVQVKWPDGKLYGAKYFGSNIAHMYQVEFEDGSQIAMKR
  EDIYTLDEELPKRVKARFSTASDMRFEDTFYGADIIQGERKRQRVLSSRFKNEYVADPIYRTFLKSSFQK
  KCQKRQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015061
• ORF Size: 3168 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015061.6
• RefSeq Size: 4687 bp
• RefSeq ORF: 3171 bp
• Locus ID: 23081
• UniProt ID: Q9H3R0
• Cytogenetics: 9p24.1
• Domains: PHD, TUDOR, JmjC, JmjN
• Protein Families: Druggable Genome, Transcription Factors
• MW: 120 kDa
• Gene Summary: This gene is a member of the Jumonji domain 2 (JMJD2) family. The encoded protein is a trimethylation-specific demethylase, and converts specific trimethylated histone residues to the dimethylated form. This enzymatic action regulates gene expression and chromosome segregation. Chromosomal aberrations and changes in expression of this gene may be found in tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]