KDM4C (NM_015061) Human Tagged ORF Clone

CAT#: RC211182

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KDM4C (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 4C (KDM4C), transcript variant 1



  "NM_015061" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 680.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol KDM4C
Synonyms GASC1; JHDM3C; JMJD2C; TDRD14C
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211182 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTGGCCGAGGTGGAAAGTCCTCTGAACCCCAGCTGTAAGATAATGACCTTCAGACCCTCCATGG
AGGAGTTCCGGGAGTTCAACAAATACCTTGCATACATGGAGTCTAAAGGAGCCCATCGTGCGGGTCTTGC
AAAGGTGATTCCTCCTAAGGAGTGGAAGCCAAGACAGTGCTATGATGACATTGATAATTTGCTCATTCCA
GCACCAATTCAGCAGATGGTCACAGGGCAGTCAGGACTGTTCACTCAGTACAACATCCAGAAAAAAGCGA
TGACTGTGAAGGAGTTCAGGCAGCTGGCCAACAGTGGCAAATATTGTACTCCAAGATACTTGGATTACGA
AGATTTGGAGCGCAAGTACTGGAAGAACTTAACTTTTGTGGCACCTATCTATGGTGCAGATATTAATGGG
AGCATATATGATGAGGGTGTGGATGAATGGAACATAGCTCGCCTCAATACAGTCTTGGATGTGGTTGAAG
AAGAGTGTGGCATTTCTATTGAGGGTGTAAATACCCCATATCTCTATTTTGGCATGTGGAAGACCACGTT
TGCATGGCACACCGAAGACATGGACCTCTATAGCATTAATTATCTCCACTTTGGAGAGCCCAAGTCTTGG
TATGCTATACCTCCGGAGCATGGAAAACGACTTGAAAGACTAGCTCAAGGTTTTTTCCCAAGCAGCTCCC
AAGGGTGTGATGCATTTCTTCGCCACAAGATGACATTGATTTCTCCATCAGTATTGAAGAAATATGGTAT
TCCCTTTGACAAGATAACCCAGGAGGCTGGAGAATTCATGATCACTTTCCCATATGGCTACCATGCTGGT
TTTAATCATGGTTTCAACTGTGCAGAATCTACAAATTTTGCTACTGTCAGATGGATTGACTATGGAAAAG
TTGCCAAATTGTGCACTTGCAGGAAAGACATGGTGAAGATTTCAATGGATATCTTTGTGAGGAAATTTCA
GCCAGACAGATATCAGCTTTGGAAACAAGGAAAGGATATATACACCATTGATCACACGAAGCCTACTCCA
GCATCCACCCCTGAAGTAAAAGCATGGCTGCAGAGGAGGAGGAAAGTAAGAAAAGCATCCCGAAGCTTCC
AGTGTGCTAGGTCTACCTCTAAAAGGCCTAAGGCTGATGAGGAAGAGGAAGTGTCAGATGAAGTCGATGG
GGCAGAGGTCCCTAACCCCGACTCAGTCACAGATGACCTCAAGGTCAGTGAAAAGTCAGAAGCAGCAGTG
AAGCTGAGGAACACAGAAGCATCTTCAGAAGAAGAGTCATCTGCTAGCAGGATGCAGGTGGAGCAGAATT
TATCAGATCATATCAAACTCTCAGGAAACAGCTGCTTAAGTACATCTGTAACAGAAGACATAAAAACTGA
GGATGACAAAGCTTATGCATATAGAAGTGTACCTTCTATATCCAGTGAGGCTGATGATTCCATTCCATTG
TCTAGTGGCTATGAGAAGCCCGAGAAATCAGACCCATCCGAGCTTTCATGGCCAAAGTCACCTGAGTCAT
GCTCATCAGTGGCAGAGAGTAATGGTGTGTTAACAGAGGGAGAAGAGAGTGATGTGGAGAGCCATGGGAA
TGGCCTTGAACCTGGGGAAATCCCAGCGGTCCCCAGTGGAGAGAGAAATAGCTTCAAAGTCCCCAGTATA
GCAGAGGGAGAGAACAAAACCTCTAAGAGTTGGCGCCATCCACTTAGCAGGCCTCCAGCAAGATCTCCGA
TGACTCTTGTGAAGCAGCAGGCGCCAAGTGATGAAGAATTGCCTGAGGTTCTGTCCATTGAGGAGGAAGT
GGAAGAAACAGAGTCTTGGGCGAAACCTCTCATCCACCTTTGGCAGACGAAGTCCCCTAACTTCGCAGCT
GAGCAAGAGTATAATGCAACAGTGGCCAGGATGAAGCCACACTGTGCCATCTGCACTCTGCTCATGCCGT
ACCACAAGCCAGATAGCAGCAATGAAGAAAATGATGCTAGATGGGAGACAAAATTAGATGAAGTCGTTAC
ATCGGAGGGAAAGACTAAGCCCCTCATACCAGAGATGTGTTTTATTTATAGTGAAGAAAATATAGAATAT
TCTCCACCCAATGCCTTCCTTGAAGAGGATGGAACAAGTCTCCTTATTTCCTGTGCAAAGTGCTGCGTAC
GGGTTCATGCAAGTTGTTATGGTATTCCTTCTCATGAGATCTGTGATGGATGGCTGTGTGCCCGGTGCAA
AAGAAATGCGTGGACAGCAGAATGCTGTCTCTGCAATTTGAGAGGAGGTGCTCTTAAGCAAACGAAGAAC
AATAAGTGGGCCCATGTCATGTGCGCCGTTGCGGTCCCAGAAGTTCGATTCACTAATGTCCCAGAAAGGA
CACAAATAGATGTAGGCAGAATACCTTTACAGAGGTTAAAATTGAAATGCATCTTCTGCAGACACCGGGT
TAAGAGGGTCTCTGGAGCCTGCATCCAGTGTTCCTACGGTCGCTGCCCGGCCTCCTTCCATGTCACTTGT
GCCCATGCTGCTGGGGTACTGATGGAGCCTGATGATTGGCCTTATGTGGTGAACATTACATGCTTTCGAC
ATAAGGTCAACCCCAACGTGAAGTCCAAGGCTTGCGAGAAGGTCATTTCCGTGGGTCAAACGGTCATCAC
GAAGCATCGGAACACCCGGTATTACAGTTGCAGAGTGATGGCTGTGACATCGCAGACCTTCTATGAGGTC
ATGTTTGATGATGGCTCCTTTAGCAGAGACACATTTCCTGAGGATATCGTGAGCCGAGACTGTCTGAAGC
TGGGCCCACCTGCTGAGGGAGAAGTCGTCCAAGTCAAGTGGCCCGATGGCAAACTCTATGGAGCAAAATA
TTTTGGATCAAATATTGCCCACATGTACCAGGTTGAGTTTGAAGATGGATCCCAGATAGCAATGAAGAGA
GAGGACATCTACACTTTAGATGAAGAGTTACCCAAGAGAGTGAAAGCTCGATTTTCCACAGCCTCTGACA
TGCGATTTGAAGACACGTTTTATGGAGCAGACATTATCCAAGGGGAGAGAAAGAGACAAAGAGTGCTGAG
CTCCAGGTTTAAGAATGAATATGTGGCCGACCCTATATACCGCACTTTTTTGAAGAGCTCTTTCCAGAAG
AAGTGCCAGAAGAGACAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211182 protein sequence
Red=Cloning site Green=Tags(s)

MEVAEVESPLNPSCKIMTFRPSMEEFREFNKYLAYMESKGAHRAGLAKVIPPKEWKPRQCYDDIDNLLIP
APIQQMVTGQSGLFTQYNIQKKAMTVKEFRQLANSGKYCTPRYLDYEDLERKYWKNLTFVAPIYGADING
SIYDEGVDEWNIARLNTVLDVVEEECGISIEGVNTPYLYFGMWKTTFAWHTEDMDLYSINYLHFGEPKSW
YAIPPEHGKRLERLAQGFFPSSSQGCDAFLRHKMTLISPSVLKKYGIPFDKITQEAGEFMITFPYGYHAG
FNHGFNCAESTNFATVRWIDYGKVAKLCTCRKDMVKISMDIFVRKFQPDRYQLWKQGKDIYTIDHTKPTP
ASTPEVKAWLQRRRKVRKASRSFQCARSTSKRPKADEEEEVSDEVDGAEVPNPDSVTDDLKVSEKSEAAV
KLRNTEASSEEESSASRMQVEQNLSDHIKLSGNSCLSTSVTEDIKTEDDKAYAYRSVPSISSEADDSIPL
SSGYEKPEKSDPSELSWPKSPESCSSVAESNGVLTEGEESDVESHGNGLEPGEIPAVPSGERNSFKVPSI
AEGENKTSKSWRHPLSRPPARSPMTLVKQQAPSDEELPEVLSIEEEVEETESWAKPLIHLWQTKSPNFAA
EQEYNATVARMKPHCAICTLLMPYHKPDSSNEENDARWETKLDEVVTSEGKTKPLIPEMCFIYSEENIEY
SPPNAFLEEDGTSLLISCAKCCVRVHASCYGIPSHEICDGWLCARCKRNAWTAECCLCNLRGGALKQTKN
NKWAHVMCAVAVPEVRFTNVPERTQIDVGRIPLQRLKLKCIFCRHRVKRVSGACIQCSYGRCPASFHVTC
AHAAGVLMEPDDWPYVVNITCFRHKVNPNVKSKACEKVISVGQTVITKHRNTRYYSCRVMAVTSQTFYEV
MFDDGSFSRDTFPEDIVSRDCLKLGPPAEGEVVQVKWPDGKLYGAKYFGSNIAHMYQVEFEDGSQIAMKR
EDIYTLDEELPKRVKARFSTASDMRFEDTFYGADIIQGERKRQRVLSSRFKNEYVADPIYRTFLKSSFQK
KCQKRQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_015061
ORF Size 3168 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015061.6
RefSeq Size 4687 bp
RefSeq ORF 3171 bp
Locus ID 23081
UniProt ID Q9H3R0
Cytogenetics 9p24.1
Domains PHD, TUDOR, JmjC, JmjN
Protein Families Druggable Genome, Transcription Factors
MW 120 kDa
Gene Summary This gene is a member of the Jumonji domain 2 (JMJD2) family. The encoded protein is a trimethylation-specific demethylase, and converts specific trimethylated histone residues to the dimethylated form. This enzymatic action regulates gene expression and chromosome segregation. Chromosomal aberrations and changes in expression of this gene may be found in tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Other Versions

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