PDE10A (NM_006661) Human Tagged ORF Clone

CAT#: RC211181

PDE10A (Myc-DDK-tagged)-Human phosphodiesterase 10A (PDE10A), transcript variant 2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Lane 1), human PDE10A transcript variant 2 (Cat# RC211181, Lane 2), human PDE10A transcript variant 1 (Cat# RC226169, Lane 3), and mouse PDE10A (Cat# MR225520, Lane 4) cDNA clones for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE10A (Cat# TA500625).

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  Western blot validation of overexpression lysate (Cat# LY416496) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211181 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PDE10A protein (Cat# TP311181). The protein was produced from HEK293T cells transfected with PDE10A cDNA clone (Cat# RC211181) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDE10A
• Synonyms: ADSD2; HSPDE10A; IOLOD; LINC00473; PDE10A19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211181 representing NM_006661
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGATAGAAGAGAGGAAATCCCAACATTTAACAGGTTTGACAGATGAAAAAGTGAAGGCATATCTTT
  CTCTTCACCCCCAGGTATTAGATGAATTTGTATCTGAAAGTGTTAGTGCAGAGACAGTAGAGAAATGGCT
  GAAGAGGAAGAACAACAAATCAGAAGATGAATCAGCTCCTAAGGAAGTCAGCAGGTACCAAGATACGAAT
  ATGCAGGGAGTTGTATATGAACTAAACAGCTATATAGAACAACGGTTGGACACAGGAGGAGACAACCAGC
  TACTCCTCTATGAACTGAGCAGCATCATTAAAATAGCCACAAAAGCCGATGGATTTGCACTGTATTTCCT
  TGGAGAGTGCAATAATAGCCTGTGTATATTCACGCCACCTGGGATAAAGGAAGGAAAACCCCGCCTCATC
  CCTGCTGGGCCCATCACTCAGGGCACCACCGTCTCTGCTTATGTGGCCAAGTCCAGGAAAACACTGCTAG
  TAGAAGACATCCTTGGAGATGAACGATTTCCAAGAGGTACTGGACTGGAATCAGGGACTCGTATCCAGTC
  TGTTCTTTGCTTACCAATTGTCACTGCAATTGGTGACTTGATTGGTATTCTCGAGCTGTATCGGCACTGG
  GGCAAAGAAGCCTTCTGTCTTAGTCACCAGGAGGTTGCAACAGCAAATCTTGCCTGGGCTTCAGTAGCAA
  TACATCAGGTGCAGGTATGCAGAGGCCTTGCCAAACAGACAGAATTGAATGACTTCCTACTCGACGTATC
  AAAAACATATTTTGATAACATAGTTGCAATAGATTCTCTACTTGAACACATAATGATATATGCAAAAAAC
  CTGGTGAATGCCGATCGTTGTGCACTTTTCCAGGTGGACCATAAGAACAAGGAGTTATATTCAGACCTTT
  TTGATATTGGAGAGGAAAAGGAAGGAAAACCTGTCTTCAAGAAGACCAAAGAGATAAGATTTTCAATTGA
  GAAAGGAATTGCTGGCCAAGTAGCAAGAACAGGGGAAGTCCTGAACATTCCAGATGCCTATGCAGACCCA
  CGCTTTAACAGAGAAGTAGACTTGTACACAGGCTACACCACGCGGAACATCCTGTGCATGCCCATCGTCA
  GCCGAGGCAGCGTGATAGGTGTGGTGCAGATGGTCAACAAAATCAGTGGCAGTGCCTTCTCTAAAACAGA
  TGAAAACAACTTCAAAATGTTTGCCGTCTTTTGTGCTTTAGCCTTACACTGTGCTAATATGTATCATAGA
  ATTCGCCACTCAGAGTGCATTTACCGGGTAACGATGGAAAAGCTGTCCTACCATAGCATTTGTACTTCAG
  AAGAGTGGCAAGGTCTCATGCAATTCACCCTTCCCGTGCGTCTCTGCAAAGAAATTGAATTATTCCACTT
  TGACATTGGTCCTTTTGAAAACATGTGGCCTGGAATTTTTGTCTACATGGTTCATCGGTCCTGTGGGACA
  TCCTGCTTTGAGCTTGAAAAGTTGTGTCGTTTTATTATGTCTGTGAAGAAGAACTATCGGCGGGTTCCTT
  ATCACAACTGGAAGCATGCGGTCACTGTAGCACACTGCATGTATGCCATACTTCAGAACAATCACACGCT
  TTTCACAGACCTTGAGCGCAAAGGACTGCTGATTGCGTGTCTGTGTCATGACCTGGACCACAGGGGCTTC
  AGTAACAGCTACCTGCAGAAGTTCGACCACCCTCTGGCCGCTCTCTACTCCACTTCCACCATGGAGCAGC
  ACCACTTCTCCCAGACTGTGTCCATCCTTCAGTTGGAAGGGCACAATATCTTCTCCACTCTGAGCTCCAG
  TGAATATGAGCAGGTGCTTGAGATCATCCGCAAAGCCATCATTGCCACAGACCTTGCTTTATACTTTGGA
  AACAGGAAGCAGTTGGAAGAGATGTACCAGACCGGATCACTAAACCTTAATAATCAATCACATAGAGACC
  GTGTAATTGGTTTGATGATGACTGCCTGTGACCTTTGTTCTGTGACAAAACTGTGGCCCGTTACAAAATT
  GACGGCAAATGATATATATGCAGAATTCTGGGCTGAGGGTGATGAAATGAAGAAATTGGGAATACAGCCT
  ATTCCTATGATGGACAGAGACAAGAAGGATGAAGTCCCCCAAGGCCAGCTTGGGTTCTACAATGCCGTGG
  CCATTCCCTGCTATACAACCCTTACCCAGATCCTCCCTCCCACGGAGCCTCTTCTGAAAGCATGCAGGGA
  TAATCTCAGTCAGTGGGAGAAGGTGATTCGAGGGGAGGAGACTGCAACCTGGATTTCATCCCCATCCGTG
  GCTCAGAAGGCAGCTGCATCTGAAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211181 representing NM_006661
  Red=Cloning site Green=Tags(s)
  
  MRIEERKSQHLTGLTDEKVKAYLSLHPQVLDEFVSESVSAETVEKWLKRKNNKSEDESAPKEVSRYQDTN
  MQGVVYELNSYIEQRLDTGGDNQLLLYELSSIIKIATKADGFALYFLGECNNSLCIFTPPGIKEGKPRLI
  PAGPITQGTTVSAYVAKSRKTLLVEDILGDERFPRGTGLESGTRIQSVLCLPIVTAIGDLIGILELYRHW
  GKEAFCLSHQEVATANLAWASVAIHQVQVCRGLAKQTELNDFLLDVSKTYFDNIVAIDSLLEHIMIYAKN
  LVNADRCALFQVDHKNKELYSDLFDIGEEKEGKPVFKKTKEIRFSIEKGIAGQVARTGEVLNIPDAYADP
  RFNREVDLYTGYTTRNILCMPIVSRGSVIGVVQMVNKISGSAFSKTDENNFKMFAVFCALALHCANMYHR
  IRHSECIYRVTMEKLSYHSICTSEEWQGLMQFTLPVRLCKEIELFHFDIGPFENMWPGIFVYMVHRSCGT
  SCFELEKLCRFIMSVKKNYRRVPYHNWKHAVTVAHCMYAILQNNHTLFTDLERKGLLIACLCHDLDHRGF
  SNSYLQKFDHPLAALYSTSTMEQHHFSQTVSILQLEGHNIFSTLSSSEYEQVLEIIRKAIIATDLALYFG
  NRKQLEEMYQTGSLNLNNQSHRDRVIGLMMTACDLCSVTKLWPVTKLTANDIYAEFWAEGDEMKKLGIQP
  IPMMDRDKKDEVPQGQLGFYNAVAIPCYTTLTQILPPTEPLLKACRDNLSQWEKVIRGEETATWISSPSV
  AQKAAASED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006661
• ORF Size: 2337 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006661.4
• RefSeq Size: 4576 bp
• RefSeq ORF: 2340 bp
• Locus ID: 10846
• UniProt ID: Q9Y233
• Cytogenetics: 6q27
• Domains: PDEase, GAF
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• MW: 88.2 kDa
• Gene Summary: The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase family. It plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This protein can hydrolyze both cAMP and cGMP to the corresponding nucleoside 5' monophosphate, but has higher affinity for cAMP, and is more efficient with cAMP as substrate. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Dec 2011]