TCEAL2 (NM_080390) Human Tagged ORF Clone

CAT#: RC211139

TCEAL2 (Myc-DDK-tagged)-Human transcription elongation factor A (SII)-like 2 (TCEAL2)

Product Images

Circular map for RC211139

• 

  Western blot validation of overexpression lysate (Cat# LY409183) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211139 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified TCEAL2 protein (Cat# TP311139). The protein was produced from HEK293T cells transfected with TCEAL2 cDNA clone (Cat# RC211139) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TCEAL2
• Synonyms: my048; MY0876G05; WEX1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211139 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAAACTCTTCAATGAAAATGAAGGAATGCCTTCGAATCAAGGAAAGATAGACAATGAAGAACAGC
  CACCGCACGAGGGAAAGCCAGAAGTAGCTTGTATTCTGGAAGACAAGAAGTTAGAAAACGAGGGAAACAC
  AGAAAACACGGGCAAGAGAGTTGAGGAACCGTTAAAGGATAAAGAAAAGCCAGAGAGTGCGGGAAAGGCA
  AAAGGAGAAGGAAAGTCAGAGAGGAAGGGAAAGTCAGAGATGCAGGGAGGATCAAAGACAGAGGGAAAGC
  CAGAGAGAGGGGGAAGGGCAGAGGGTGAAGGAGAGCCAGACAGTGAAAGAGAGCCAGAGAGTGAGGGAGA
  GCCAGAAAGTGAAACAAGGGCTGCAGGAAAGCGCCCAGCTGAGGATGATATACCCAGGAAAGCCAAAAGA
  AAAACCAACAAGGGGCTGGCTCAGTACCTCAAGCAATATAAGGAAGCCATACATGATATGAATTTCAGCA
  ATGAGGACATGATAAGAGAATTTGACAACATGGCTAGGGTGGAGGATAAAAGGAGAAAAAGCAAACAGAA
  ATTGGGGGCGTTTTTGTGGATGCAAAGAAATTTACAGGACCCCTTCTATCCTAGGGGTCCAAGGGAATTC
  AGGGGTGGCTGCAGGGCCCCACGAAGGGACACTGAAGACATTCCTTATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211139 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEKLFNENEGMPSNQGKIDNEEQPPHEGKPEVACILEDKKLENEGNTENTGKRVEEPLKDKEKPESAGKA
  KGEGKSERKGKSEMQGGSKTEGKPERGGRAEGEGEPDSEREPESEGEPESETRAAGKRPAEDDIPRKAKR
  KTNKGLAQYLKQYKEAIHDMNFSNEDMIREFDNMARVEDKRRKSKQKLGAFLWMQRNLQDPFYPRGPREF
  RGGCRAPRRDTEDIPYV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080390
• ORF Size: 681 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080390.4
• RefSeq Size: 1123 bp
• RefSeq ORF: 684 bp
• Locus ID: 140597
• UniProt ID: Q9H3H9
• Cytogenetics: Xq22.1
• MW: 25.8 kDa
• Gene Summary: This gene encodes a member of the transcription elongation factor A (SII)-like (TCEAL) gene family. Members of this family contain TFA domains and may function as nuclear phosphoproteins that modulate transcription in a promoter context-dependent manner. Multiple family members are located on the X chromosome. [provided by RefSeq, Jul 2008]