INPP4B (NM_003866) Human Tagged ORF Clone

CAT#: RC211134

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INPP4B (Myc-DDK-tagged)-Human inositol polyphosphate-4-phosphatase, type II, 105kDa (INPP4B), transcript variant 1



  "NM_003866" in other vectors (6)

Reconstitution Protocol

USD 1,269.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol INPP4B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC211134 representing NM_003866
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAATTAAAGAGGAAGGGGCATCAGAAGAAGGGCAGCACTTTCTTCCTACAGCCCAGGCCAATGATC
CCGGGGACTGTCAGTTCACAAGTATCCAGAAGACTCCAAATGAACCGCAGTTGGAATTCATCCTTGCATG
CAAGGATCTCGTGGCTCCTGTCCGTGATCGTAAACTGAATACACTGGTGCAGATCTCCGTAATCCACCCC
GTGGAGCAGAGTCTGACAAGATACTCCAGCACCGAAATTGTGGAGGGAACAAGGGACCCACTGTTTTTGA
CTGGTGTCACATTCCCATCTGAGTATCCCATCTATGAGGAGACCAAAATAAAACTAACAGTCTATGATGT
CAAGGATAAGTCTCATGACACCGTTCGAACCAGTGTCCTACCAGAACATAAGGATCCCCCGCCAGAAGTT
GGGCGAAGTTTCTTGGGCTATGCCAGTTTTAAAGTGGGAGAGCTGCTGAAGTCAAAGGAGCAATTGCTGG
TCCTGAGCCTGAGAACTTCAGATGGTGGCAAAGTGGTTGGCACCATAGAAGTCAGTGTCGTGAAGATGGG
GGAGATTGAGGATGGGGAAGCCGACCACATCACCACAGATGTACAGGGACAAAAGTGTGCCCTGGTATGT
GAATGTACAGCCCCGGAAAGTGTGAGCGGAAAAGATAACTTACCTTTTTTGAATTCAGTGTTAAAGAACC
CAGTATGTAAATTATATAGATTTCCCACATCTGACAATAAGTGGATGCGAATTCGAGAGCAGATGTCAGA
GAGCATTCTTTCCTTTCATATTCCTAAGGAATTGATTTCCCTTCACATTAAAGAAGATTTGTGCAGAAAC
CAGGAGATAAAAGAACTTGGTGAGCTTTCTCCACATTGGGACAATCTGCGAAAAAATGTCCTTACTCACT
GTGATCAAATGGTGAATATGTACCAAGACATTCTGACAGAACTTAGCAAGGAAACAGGGTCCTCTTTCAA
ATCAAGCAGCAGCAAAGGAGAGAAAACATTAGAATTTGTTCCAATAAATCTACATCTGCAAAGAATGCAG
GTACACAGCCCTCACTTGAAAGATGCTCTCTACGATGTCATCACTGTGGGAGCCCCAGCTGCCCATTTTC
AGGGATTTAAGAATGGTGGTCTTCGGAAGCTACTCCATAGATTTGAAACAGAAAGAAGAAATACCGGATA
CCAGTTTATTTACTATTCACCTGAAAACACAGCCAAAGCAAAGGAAGTTCTCAGCAACATCAATCAACTA
CAACCTCTTATAGCAACCCATGCAGACCTACTGCTTAATTCTGCAAGCCAGCATTCTCCAGACAGCTTGA
AGAATTCTTTAAAGATGCTTTCAGAAAAAACAGAGCTTTTTGTACATGCCTTCAAGGATCAACTTGTCAG
GAGTGCTCTTTTAGCACTCTACACTGCAAGGCCAGGAGGCATTCTTAAGAAGCCACCCTCTCCTAAGAGC
AGCACAGAGGAGAGCAGTCCCCAAGACCAACCCCCAGTGATGAGAGGGCAGGACTCCATACCACATCATT
CAGACTATGATGAGGAAGAGTGGGACAGGGTGTGGGCCAATGTGGGGAAGAGCCTGAACTGCATTATTGC
TATGGTGGACAAACTGATTGAAAGAGATGGTGGCAGTGAAGGCAGTGGCGGCAACAATGATGGAGAAAAG
GAACCTTCATTAACAGATGCCATTCCCTCTCACCCAAGAGAGGACTGGTATGAACAGTTGTATCCCCTCA
TCCTTACCCTGAAGGACTGCATGGGAGAAGTGGTGAACCGAGCCAAGCAGTCCCTGACATTTGTGCTCCT
TCAGGAACTTGCGTACAGCTTGCCCCAGTGTCTGATGCTGACGCTAAGAAGAGACATCGTCTTCAGCCAA
GCACTTGCTGGATTGGTTTGTGGTTTTATCATCAAATTACAGACAAGTCTGTATGACCCAGGCTTCCTAC
AGCAGCTTCACACAGTGGGGTTGATAGTACAATATGAAGGACTGCTAAGTACATACAGCGATGAAATTGG
AATGCTAGAGGACATGGCCGTTGGCATTTCCGATTTAAAGAAAGTTGCATTTAAAATAATTGAAGCCAAA
TCCAATGATGTGTTGCCAGTTATAACAGGAAGACGAGAACATTACGTGGTAGAGGTCAAGCTTCCAGCCA
GAATGTTTGAGTCACTACCTCTACAGATTAAAGAAGGACAGTTGCTTCATGTGTATCCAGTACTTTTTAA
TGTTGGAATCAATGAACAGCAAACTCTGGCTGAAAGGTTTGGAGATGTCTCTTTGCAAGAAAGTATTAAT
CAGGAAAACTTCGAACTTCTACAAGAATATTACAAGATATTTATGGAAAAGATGCCTCCTGATTATATTT
CACATTTTCAGGAACAAAATGATTTAAAAGCATTGCTAGAAAATCTCCTTCAAAATATCCAATCCAAAAA
AAGAAAGAATGTAGAAATTATGTGGCTGGCTGCAACGATTTGCCGCAAACTGAATGGTATTCGTTTCACC
TGTTGTAAAAGTGCCAAAGACAGGACATCGATGTCAGTGACACTTGAACAATGCTCAATCTTGAGAGATG
AGCACCAGTTACACAAGGACTTCTTTATCCGAGCGCTGGATTGCATGAGAAGAGAAGGATGCCGCATAGA
GAATGTACTGAAGAATATCAAATGCAGAAAGTATGCTTTCAACATGCTACAGCTGATGGCTTTCCCCAAG
TACTACAGACCTCCAGAGGGGACTTATGGAAAAGCTGACACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC211134 representing NM_003866
Red=Cloning site Green=Tags(s)

MEIKEEGASEEGQHFLPTAQANDPGDCQFTSIQKTPNEPQLEFILACKDLVAPVRDRKLNTLVQISVIHP
VEQSLTRYSSTEIVEGTRDPLFLTGVTFPSEYPIYEETKIKLTVYDVKDKSHDTVRTSVLPEHKDPPPEV
GRSFLGYASFKVGELLKSKEQLLVLSLRTSDGGKVVGTIEVSVVKMGEIEDGEADHITTDVQGQKCALVC
ECTAPESVSGKDNLPFLNSVLKNPVCKLYRFPTSDNKWMRIREQMSESILSFHIPKELISLHIKEDLCRN
QEIKELGELSPHWDNLRKNVLTHCDQMVNMYQDILTELSKETGSSFKSSSSKGEKTLEFVPINLHLQRMQ
VHSPHLKDALYDVITVGAPAAHFQGFKNGGLRKLLHRFETERRNTGYQFIYYSPENTAKAKEVLSNINQL
QPLIATHADLLLNSASQHSPDSLKNSLKMLSEKTELFVHAFKDQLVRSALLALYTARPGGILKKPPSPKS
STEESSPQDQPPVMRGQDSIPHHSDYDEEEWDRVWANVGKSLNCIIAMVDKLIERDGGSEGSGGNNDGEK
EPSLTDAIPSHPREDWYEQLYPLILTLKDCMGEVVNRAKQSLTFVLLQELAYSLPQCLMLTLRRDIVFSQ
ALAGLVCGFIIKLQTSLYDPGFLQQLHTVGLIVQYEGLLSTYSDEIGMLEDMAVGISDLKKVAFKIIEAK
SNDVLPVITGRREHYVVEVKLPARMFESLPLQIKEGQLLHVYPVLFNVGINEQQTLAERFGDVSLQESIN
QENFELLQEYYKIFMEKMPPDYISHFQEQNDLKALLENLLQNIQSKKRKNVEIMWLAATICRKLNGIRFT
CCKSAKDRTSMSVTLEQCSILRDEHQLHKDFFIRALDCMRREGCRIENVLKNIKCRKYAFNMLQLMAFPK
YYRPPEGTYGKADT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003866
ORF Size 2772 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003866.3
RefSeq Size 2937 bp
RefSeq ORF 2775 bp
Locus ID 8821
UniProt ID O15327
Cytogenetics 4q31.21
Protein Families Druggable Genome
Protein Pathways Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
MW 104.6 kDa
Gene Summary INPP4B encodes the inositol polyphosphate 4-phosphatase type II, one of the enzymes involved in phosphatidylinositol signaling pathways. This enzyme removes the phosphate group at position 4 of the inositol ring from inositol 3,4-bisphosphate. There is limited data to suggest that the human type II enzyme is subject to alternative splicing, as has been established for the type I enzyme. [provided by RefSeq, Jul 2008]

Other Versions

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