NLRX1 (NM_024618) Human Tagged ORF Clone

CAT#: RC211111

NLRX1 (Myc-DDK-tagged)-Human NLR family member X1 (NLRX1), transcript variant 1

Product Images

Circular map for RC211111

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NLRX1 (Cat# RC211111, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NLRX1 (Cat# TA809764)(1:2000). Positive lysates LY403011 (100ug) and LC403011 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY403011) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211111 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NLRX1
• Synonyms: CLR11.3; DLNB26; NOD5; NOD9; NOD26
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211111 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTGGGGCCACCATTTGCCCAGGGCCTCTTGGGGCTCTGGTTTTAGAAGAGCACTCCAGCGACCAG
  ATGATCGTATCCCCTTCCTGATCCACTGGAGTTGGCCCCTTCAAGGGGAGCGTCCCTTTGGGCCCCCTAG
  GGCCTTTATACGCCACCACGGAAGCTCGGTAGATAGCGCTCCCCCACCCGGGAGGCATGGACGGCTGTTC
  CCCAGCGCCTCTGCAACTGAAGCTATACAGCGGCACCGCCGGAACCTGGCTGAGTGGTTCAGCCGGCTGC
  CCAGGGAGGAGCGCCAGTTTGGCCCAACCTTTGCCCTAGACACGGTCCACGTTGACCCTGTGATCCGCGA
  GAGTACCCCTGATGAGCTACTTCGCCCACCCGCGGAGCTGGCCCTGGAGCATCAGCCACCCCAGGCCGGG
  CTCCCCCCACTGGCCTTGTCTCAGCTCTTTAACCCGGATGCCTGTGGGCGCCGGGTGCAGACAGTGGTGC
  TGTATGGGACAGTGGGCACAGGCAAGAGCACGCTGGTGCGCAAGATGGTTCTGGACTGGTGTTATGGGCG
  GCTGCCGGCCTTCGAGCTGCTCATCCCCTTCTCCTGTGAGGACCTGTCATCCCTGGGCCCTGCCCCAGCC
  TCCCTGTGCCAACTTGTGGCCCAGCGCTACACGCCCCTGAAGGAGGTTCTGCCCCTGATGGCTGCTGCTG
  GGTCCCACCTCCTCTTTGTGCTCCATGGCTTAGAGCATCTCAACCTCGACTTCCGGCTGGCAGGCACGGG
  ACTTTGTAGTGACCCGGAGGAACCGCAGGAACCAGCTGCTATCATCGTCAACCTGCTGCGCAAATACATG
  CTGCCTCAGGCCAGCATTCTGGTGACCACTCGGCCCTCTGCCATTGGCCGTATCCCCAGCAAGTACGTGG
  GCCGCTATGGTGAGATCTGCGGTTTCTCTGATACCAACCTGCAGAAGCTCTACTTCCAGCTCCGCCTCAA
  CCAGCCGTACTGCGGGTATGCCGTTGGCGGTTCAGGTGTCTCTGCCACACCAGCTCAGCGTGACCACCTG
  GTGCAGATGCTCTCCCGGAACCTGGAGGGGCACCACCAGATAGCCGCTGCCTGCTTCCTGCCGTCCTATT
  GCTGGCTCGTTTGTGCCACCTTGCACTTCCTGCATGCCCCCACGCCTGCTGGGCAGACCCTTACAAGCAT
  CTATACCAGCTTCCTGCGCCTCAACTTCAGCGGGGAAACCCTGGACAGCACTGACCCCTCCAATTTGTCC
  CTGATGGCCTATGCAGCCCGAACCATGGGCAAGTTGGCCTATGAGGGGGTGTCCTCCCGCAAGACCTACT
  TCTCTGAAGAGGATGTCTGTGGCTGCCTGGAGGCTGGCATCAGGACGGAGGAGGAGTTTCAGCTGCTGCA
  CATCTTCCGTCGGGATGCCCTGAGGTTTTTCCTGGCCCCATGTGTGGAGCCAGGGCGTGCAGGCACCTTC
  GTGTTCACCGTGCCCGCCATGCAGGAATACCTGGCTGCCCTCTACATTGTGCTGGGTTTGCGCAAGACGA
  CCCTGCAAAAGGTGGGCAAGGAAGTGGCTGAGCTCGTGGGCCGTGTTGGGGAGGACGTCAGCCTGGTACT
  GGGCATCATGGCCAAGCTGCTGCCTCTGCGGGCTCTGCCTCTGCTCTTCAACCTGATCAAGGTGGTTCCA
  CGAGTGTTTGGGCGCATGGTGGGTAAAAGCCGGGAGGCGGTGGCTCAGGCCATGGTGCTGGAGATGTTTC
  GAGAGGAGGACTACTACAACGATGATGTTCTGGACCAGATGGGCGCCAGTATCCTGGGCGTGGAGGGCCC
  CCGGCGCCACCCAGATGAGCCCCCTGAGGATGAAGTCTTCGAGCTCTTCCCCATGTTCATGGGGGGGCTT
  CTCTCTGCCCACAACCGAGCTGTGCTAGCTCAGCTTGGCTGCCCCATCAAGAACCTGGATGCCCTGGAGA
  ATGCCCAGGCCATCAAGAAGAAGCTGGGCAAGCTGGGCCGGCAGGTGCTGCCCCCATCAGAGCTCCTTGA
  CCACCTCTTCTTCCACTATGAGTTCCAGAACCAGCGCTTCTCCGCTGAGGTGCTCAGCTCCCTGCGTCAG
  CTCAACCTGGCAGGTGTGCGCATGACACCAGTCAAGTGCACAGTGGTGGCAGCTGTGCTGGGCAGCGGAA
  GGCATGCCCTGGATGAGGTGAACTTGGCCTCCTGCCAGCTAGATCCTGCTGGGCTGCGCACACTCCTGCC
  TGTCTTCCTGCGTGCCCGGAAGCTGGGCTTGCAACTCAACAGCCTGGGCCCTGAGGCCTGCAAGGACCTC
  CGAGACCTGTTGCTGCATGACCAGTGCCAAATTACCACACTGCGGCTGTCCAACAACCCGCTGACGGCGG
  CAGGTGTTGCCGTGCTAATGGAGGGGCTGGCAGGAAACACCTCAGTGACGCACCTGTCCCTGCTGCACAC
  GGGCCTTGGGGACGAAGGCCTGGAGCTGCTGGCTGCCCAGCTGGACCGCAACCGGCAGCTGCAGGAGCTG
  AACGTGGCGTACAACGGTGCTGGTGACACAGCGGCCCTGGCCCTGGCCAGAGCTGCCCGGGAGCACCCTT
  CCCTGGAACTGCTACACCTCTACTTCAATGAGCTGAGCTCAGAGGGCCGCCAGGTCTTGCGAGACTTGGG
  GGGTGCTGCTGAAGGTGGTGCCCGGGTGGTGGTGTCACTGACAGAGGGGACGGCGGTGTCAGAATACTGG
  TCAGTGATCCTCAGTGAAGTCCAGCGGAACCTCAATAGCTGGGATCGGGCCCGGGTTCAGCGACACCTTG
  AGCTCCTACTGCGGGATCTGGAAGATAGCCGGGGTGCCACCCTTAATCCTTGGCGCAAGGCCCAGCTGCT
  GCGAGTGGAGGGCGAGGTCAGGGCCCTCCTGGAGCAGCTGGGAAGCTCTGGAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211111 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRWGHHLPRASWGSGFRRALQRPDDRIPFLIHWSWPLQGERPFGPPRAFIRHHGSSVDSAPPPGRHGRLF
  PSASATEAIQRHRRNLAEWFSRLPREERQFGPTFALDTVHVDPVIRESTPDELLRPPAELALEHQPPQAG
  LPPLALSQLFNPDACGRRVQTVVLYGTVGTGKSTLVRKMVLDWCYGRLPAFELLIPFSCEDLSSLGPAPA
  SLCQLVAQRYTPLKEVLPLMAAAGSHLLFVLHGLEHLNLDFRLAGTGLCSDPEEPQEPAAIIVNLLRKYM
  LPQASILVTTRPSAIGRIPSKYVGRYGEICGFSDTNLQKLYFQLRLNQPYCGYAVGGSGVSATPAQRDHL
  VQMLSRNLEGHHQIAAACFLPSYCWLVCATLHFLHAPTPAGQTLTSIYTSFLRLNFSGETLDSTDPSNLS
  LMAYAARTMGKLAYEGVSSRKTYFSEEDVCGCLEAGIRTEEEFQLLHIFRRDALRFFLAPCVEPGRAGTF
  VFTVPAMQEYLAALYIVLGLRKTTLQKVGKEVAELVGRVGEDVSLVLGIMAKLLPLRALPLLFNLIKVVP
  RVFGRMVGKSREAVAQAMVLEMFREEDYYNDDVLDQMGASILGVEGPRRHPDEPPEDEVFELFPMFMGGL
  LSAHNRAVLAQLGCPIKNLDALENAQAIKKKLGKLGRQVLPPSELLDHLFFHYEFQNQRFSAEVLSSLRQ
  LNLAGVRMTPVKCTVVAAVLGSGRHALDEVNLASCQLDPAGLRTLLPVFLRARKLGLQLNSLGPEACKDL
  RDLLLHDQCQITTLRLSNNPLTAAGVAVLMEGLAGNTSVTHLSLLHTGLGDEGLELLAAQLDRNRQLQEL
  NVAYNGAGDTAALALARAAREHPSLELLHLYFNELSSEGRQVLRDLGGAAEGGARVVVSLTEGTAVSEYW
  SVILSEVQRNLNSWDRARVQRHLELLLRDLEDSRGATLNPWRKAQLLRVEGEVRALLEQLGSSGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024618
• ORF Size: 2925 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024618.4
• RefSeq Size: 3777 bp
• RefSeq ORF: 2928 bp
• Locus ID: 79671
• UniProt ID: Q86UT6
• Cytogenetics: 11q23.3
• Domains: LRR, LRR_RI
• Protein Pathways: RIG-I-like receptor signaling pathway
• MW: 107.6 kDa
• Gene Summary: The protein encoded by this gene is a member of the NLR family and localizes to the outer mitochondrial membrane. The encoded protein is a regulator of mitochondrial antivirus responses. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2013]