CDYL (NM_170751) Human Tagged ORF Clone

CAT#: RC211110

CDYL (Myc-DDK-tagged)-Human chromodomain protein, Y-like (CDYL), transcript variant 2

Product Images

Circular map for RC211110

• 

  Western blot validation of overexpression lysate (Cat# LY406868) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211110 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDYL
• Synonyms: CDYL1, MGC131936, DKFZp586C1622
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211110 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCGAGGAGCTGTACGAGGTTGAAAGGATTGTTGACAAAAGGAAAAATAAAAAAGGGAAGACAG
  AGTATTTGGTTCGGTGGAAAGGCTATGACAGCGAGGACGACACTTGGGAGCCGGAACAGCACCTCGTGAA
  CTGTGAGGAATACATCCACGACTTCAACAGACGCCACACGGAGAAGCAGAAGGAGAGCACATTGACCAGA
  ACAAACAGGACCTCTCCCAACAATGCTAGGAAACAAATCTCCAGATCCACCAACAGCAACTTTTCTAAGA
  CCTCTCCTAAGGCACTCGTGATTGGGAAAGACCACGAATCCAAAAACAGCCAGCTGTTTGCTGCCAGCCA
  GAAGTTCAGGAAGAACACAGCTCCATCTCTCTCCAGCCGGAAGAACATGGACCTAGCGAAGTCAGGTATC
  AAGATCCTCGTGCCTAAAAGCCCCGTTAAGAGCAGGACCGCAGTGGACGGCTTTCAGAGCGAGAGCCCTG
  AGAAACTGGACCCCGTCGAGCAGGGTCAGGAGGACACAGTGGCACCCGAAGTGGCAGCGGAAAAGCCGGT
  CGGAGCTTTATTGGGCCCCGGTGCCGAGAGGGCCAGGATGGGGAGCAGGCCCAGGATACACCCACTAGTG
  CCTCAGGTGCCCGGCCCTGTGACTGCAGCCATGGCCACAGGCTTAGCTGTTAACGGGAAAGGTACATCTC
  CGTTCATGGATGCATTAACAGCCAATGGGACAACCAACATACAGACATCTGTTACAGGAGTGACTGCCAG
  CAAAAGGAAATTTATTGACGACAGAAGAGACCAGCCTTTTGACAAGCGATTGCGTTTCAGCGTGAGGCAA
  ACAGAAAGTGCCTACAGATACAGAGATATTGTGGTCAGGAAGCAGGATGGCTTCACCCACATCTTGTTAT
  CCACAAAGTCCTCAGAGAATAACTCACTAAATCCAGAGGTAATGAGAGAAGTCCAGAGTGCTCTGAGCAC
  GGCCGCTGCCGATGACAGCAAGCTGGTACTGCTCAGCGCCGTTGGCAGCGTCTTCTGTTGTGGACTTGAC
  TTTATTTATTTTATACGACGTCTGACAGATGACAGGAAAAGAGAAAGCACTAAAATGGCAGAAGCTATCA
  GAAACTTCGTGAATACTTTCATTCAATTTAAGAAGCCCATTATTGTAGCAGTCAATGGCCCAGCCATTGG
  TCTAGGAGCATCTATATTGCCTCTTTGCGATGTGGTTTGGGCTAATGAAAAGGCTTGGTTTCAAACACCC
  TATACCACCTTCGGACAGAGTCCAGATGGCTGTTCTACCGTTATGTTTCCCAAGATAATGGGAGGAGCAT
  CTGCAAACGAGATGCTGCTCAGTGGACGGAAGCTGACAGCGCAGGAGGCGTGTGGCAAGGGCCTGGTCTC
  CCAGGTGTTTTGGCCCGGGACGTTCACTCAGGAAGTGATGGTTCGCATTAAGGAGCTTGCCTCGTGCAAT
  CCAGTTGTGCTTGAGGAATCCAAAGCCCTCGTGCGCTGCAACATGAAGATGGAGCTGGAGCAGGCCAACG
  AGAGGGAGTGTGAGGTGCTGAAGAAAATCTGGGGCTCGGCCCAGGGGATGGACTCCATGTTAAAGTACTT
  GCAGAGGAAGATCGATGAGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211110 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASEELYEVERIVDKRKNKKGKTEYLVRWKGYDSEDDTWEPEQHLVNCEEYIHDFNRRHTEKQKESTLTR
  TNRTSPNNARKQISRSTNSNFSKTSPKALVIGKDHESKNSQLFAASQKFRKNTAPSLSSRKNMDLAKSGI
  KILVPKSPVKSRTAVDGFQSESPEKLDPVEQGQEDTVAPEVAAEKPVGALLGPGAERARMGSRPRIHPLV
  PQVPGPVTAAMATGLAVNGKGTSPFMDALTANGTTNIQTSVTGVTASKRKFIDDRRDQPFDKRLRFSVRQ
  TESAYRYRDIVVRKQDGFTHILLSTKSSENNSLNPEVMREVQSALSTAAADDSKLVLLSAVGSVFCCGLD
  FIYFIRRLTDDRKRESTKMAEAIRNFVNTFIQFKKPIIVAVNGPAIGLGASILPLCDVVWANEKAWFQTP
  YTTFGQSPDGCSTVMFPKIMGGASANEMLLSGRKLTAQEACGKGLVSQVFWPGTFTQEVMVRIKELASCN
  PVVLEESKALVRCNMKMELEQANERECEVLKKIWGSAQGMDSMLKYLQRKIDEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_170751
• ORF Size: 1632 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170751.1, NP_736607.1
• RefSeq Size: 3477 bp
• RefSeq ORF: 1634 bp
• Locus ID: 9425
• Cytogenetics: 6p25.1
• MW: 60.6 kDa
• Gene Summary: Chromodomain Y is a primate-specific Y-chromosomal gene family expressed exclusively in the testis and implicated in infertility. Although the Y-linked genes are testis-specific, this autosomal gene is ubiquitously expressed. The Y-linked genes arose by retrotransposition of an mRNA from this gene, followed by amplification of the retroposed gene. Proteins encoded by this gene superfamily possess a chromodomain, a motif implicated in chromatin binding and gene suppression, and a catalytic domain believed to be involved in histone acetylation. Multiple proteins are encoded by transcript variants of this gene. [provided by RefSeq, Jul 2008]