TMEM195 (AGMO) (NM_001004320) Human Tagged ORF Clone

CAT#: RC211070

AGMO (Myc-DDK-tagged)-Human alkylglycerol monooxygenase (AGMO)

Product Images

Circular map for RC211070

• 

  Western blot validation of overexpression lysate (Cat# LY423724) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC211070 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM195
• Synonyms: TMEM195
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC211070 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAACCCAGAAGCCCAGCAGGATGTTTCAGTTTCCCAGGGATTTCGCATGTTGTTTTACACGATGA
  AACCCAGTGAAACTTCATTCCAAACATTAGAAGAGGTGCCTGATTATGTAAAAAAGGCAACTCCATTTTT
  CATTTCTTTGATGCTGCTTGAACTTGTTGTCAGCTGGATTCTCAAAGGAAAGCCACCAGGTCGCCTGGAT
  GATGCTTTAACGTCAATCTCAGCTGGTGTTCTGTCTCGACTTCCAAGTCTATTTTTCAGGAGCATTGAAC
  TGACCAGTTATATTTATATCTGGGAGAACTACAGGCTGTTCAATTTGCCTTGGGATTCTCCATGGACTTG
  GTATTCAGCCTTCTTAGGAGTTGACTTTGGCTACTACTGGTTCCATCGTATGGCTCATGAAGTTAATATT
  ATGTGGGCCGGACACCAAACACATCATAGTTCTGAAGACTATAACTTATCCACAGCACTGAGACAGTCTG
  TCCTCCAGATATATACTTCCTGGATTTTCTACTCTCCCCTGGCCCTCTTCATACCCCCTTCAGTATATGC
  TGTTCATCTTCAATTCAATCTTCTTTACCAATTTTGGATCCATACAGAGGTCATCAATAACCTTGGTCCT
  TTGGAACTGATTCTTAATACTCCTAGCCATCATAGGGTTCATCATGGCAGAAATCGTTATTGCATAGACA
  AAAATTATGCTGGTGTTCTTATTATTTGGGATAAAATTTTTGGGACATTTGAAGCAGAAAATGAAAAAGT
  TGTATATGGCTTAACACATCCCATTAATACATTTGAACCAATCAAAGTGCAGTTCCATCACTTATTTTCC
  ATATGGACTACATTCTGGGCCACACCTGGATTCTTCAATAAGTTTTCTGTCATATTTAAGGGACCGGGAT
  GGGGTCCAGGTAAACCAAGACTTGGTCTCAGTGAAGAAATTCCAGAGGTCACCGGCAAAGAAGTTCCCTT
  CTCATCATCTTCATCTCAGCTATTAAAGATATATACAGTTGTACAGTTTGCTCTGATGTTGGCATTTTAT
  GAAGAGACCTTTGCAGATACAGCTGCACTGTCGCAAGTTACTCTCCTTCTGAGGGTTTGCTTCATTATCC
  TGACCTTGACTTCCATTGGATTTCTTCTGGATCAAAGACCCAAGGCAGCTATTATGGAAACTCTCCGTTG
  CTTGATGTTCTTAATGCTGTACCGATTTGGTCACCTGAAGCCTCTTGTCCCTTCATTGTCATCTGCTTTT
  GAGATTGTTTTTTCCATTTGCATTGCTTTCTGGGGAGTTAGAAGCATGAAACAACTCACCTCTCACCCTT
  GGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC211070 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKNPEAQQDVSVSQGFRMLFYTMKPSETSFQTLEEVPDYVKKATPFFISLMLLELVVSWILKGKPPGRLD
  DALTSISAGVLSRLPSLFFRSIELTSYIYIWENYRLFNLPWDSPWTWYSAFLGVDFGYYWFHRMAHEVNI
  MWAGHQTHHSSEDYNLSTALRQSVLQIYTSWIFYSPLALFIPPSVYAVHLQFNLLYQFWIHTEVINNLGP
  LELILNTPSHHRVHHGRNRYCIDKNYAGVLIIWDKIFGTFEAENEKVVYGLTHPINTFEPIKVQFHHLFS
  IWTTFWATPGFFNKFSVIFKGPGWGPGKPRLGLSEEIPEVTGKEVPFSSSSSQLLKIYTVVQFALMLAFY
  EETFADTAALSQVTLLLRVCFIILTLTSIGFLLDQRPKAAIMETLRCLMFLMLYRFGHLKPLVPSLSSAF
  EIVFSICIAFWGVRSMKQLTSHPWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004320
• ORF Size: 1335 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004320.2
• RefSeq Size: 2475 bp
• RefSeq ORF: 1338 bp
• Locus ID: 392636
• UniProt ID: Q6ZNB7
• Cytogenetics: 7p21.2
• Protein Families: Transmembrane
• MW: 51.5 kDa
• Gene Summary: The protein encoded by this gene is a tetrahydrobiopterin- and iron-dependent enzyme that cleaves the ether bond of alkylglycerols. Sequence comparisons distinguish this protein as forming a third, distinct class of tetrahydrobiopterin-dependent enzymes. Variations in this gene have been associated with decreased glucose-stimulated insulin response, type 2 diabetes, and susceptibility to intracranial aneurysms. [provided by RefSeq, Aug 2012]