PTGIS (NM_000961) Human Tagged Lenti ORF Clone

CAT#: RC211030L4

  • LentiORF®

Lenti ORF clone of Human prostaglandin I2 (prostacyclin) synthase (PTGIS), mGFP tagged


  "NM_000961" in other vectors (6)

Reconstitution Protocol

USD 757.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
Lenti-vpak packaging kit - packaging plasmids and transfection reagent
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Lenti ORF control particles of pLenti-C-mGFP-P2A-Puro, >1x10^7 TU/ml, 0.5 ml
    • 500 ul

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Forward primer to sequence targets cloned in expression vectors.
    • 1 nmol

USD 71.00


Reverse primer to sequence targets cloned in expression vectors.
    • 1 nmol

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PTGIS rabbit polyclonal antibody
    • 100 ul

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Other products for "PTGIS"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag mGFP
Symbol PTGIS
Synonyms CYP8; CYP8A1; PGIS; PTGI
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
The ORF insert of this clone is exactly the same as(RC211030).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000961
ORF Size 1500 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000961.3
RefSeq Size 5603 bp
RefSeq ORF 1503 bp
Locus ID 5740
UniProt ID Q16647
Cytogenetics 20q13.13
Protein Families Druggable Genome, P450, Transmembrane
Protein Pathways Arachidonic acid metabolism, Metabolic pathways
MW 57.1 kDa
Gene Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to prostacyclin (prostaglandin I2), a potent vasodilator and inhibitor of platelet aggregation. An imbalance of prostacyclin and its physiological antagonist thromboxane A2 contribute to the development of myocardial infarction, stroke, and atherosclerosis. [provided by RefSeq, Jul 2008]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.