MCM10 (NM_018518) Human Tagged ORF Clone

CAT#: RC210997

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MCM10 (Myc-DDK-tagged)-Human minichromosome maintenance complex component 10 (MCM10), transcript variant 2



  "NM_018518" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MCM10
Synonyms CNA43; DNA43; PRO2249
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210997 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGAGGAGGAAGACAATCTGTCTCTGCTGACCGCACTGCTGGAAGAAAATGAGTCAGCCTTGGATT
GTAATTCAGAAGAAAATAACTTCTTGACGCGGGAAAATGGCGAGCCCGACGCATTTGATGAGCTCTTTGA
TGCCGACGGCGACGGTGAATCTTATACAGAAGAGGCTGATGATGGAGAAACAGGAGAGACAAGAGACGAA
AAGGAAAATCTGGCCACTCTCTTTGGAGATATGGAGGACTTAACAGATGAAGAAGAAGTTCCCGCATCAC
AGTCAACTGAAAATAGGGTCCTCCCTGCTCCTGCCCCCAGGCGAGAGAAAACGAATGAAGAGTTGCAAGA
GGAATTAAGGAATTTGCAAGAGCAAATGAAGGCCTTACAAGAGCAGCTAAAAGTAACAACAATTAAACAG
ACAGCAAGCCCAGCCCGTCTGCAAAAATCCCCTGTAGAGAAGTCTCCCCGGCCACCTCTTAAGGAGAGGA
GAGTTCAGAGAATTCAGGAGTCAACATGCTTTTCTGCGGAGCTTGATGTCCCTGCGCTACCAAGAACCAA
GAGGGTGGCTCGAACACCAAAGGCTTCACCTCCAGATCCCAAAAGCTCATCTTCAAGGATGACAAGTGCA
CCCTCCCAACCCCTACAGACGATTTCTCGGAACAAACCTAGTGGGATAACTAGAGGTCAAATTGTGGGGA
CCCCAGGAAGTTCTGGGGAAACGACTCAACCCATCTGTGTGGAAGCCTTCTCTGGTCTGCGGCTCAGGCG
GCCTCGAGTATCCTCCACAGAAATGAACAAGAAAATGACCGGCCGAAAACTGATCAGACTGTCTCAGATC
AAGGAAAAGATGGCCAGAGAGAAGCTGGAAGAAATAGATTGGGTGACATTTGGGGTTATATTGAAGAAGG
TTACGCCACAGAGTGTGAATAGTGGAAAAACCTTCAGCATATGGAAACTGAATGATCTTCGTGACCTGAC
ACAATGTGTGTCCTTGTTCTTATTTGGAGAAGTTCACAAAGCGCTCTGGAAGACGGAGCAGGGGACTGTC
GTAGGGATCCTCAATGCCAACCCCATGAAGCCCAAGGATGGTTCAGAGGAGGTGTGTTTATCTATCGATC
ATCCTCAGAAGGTCTTAATTATGGGTGAAGCTCTTGACCTGGGAACCTGTAAAGCCAAGAAGAAGAATGG
AGAGCCGTGCACGCAGACTGTGAATTTGCGTGACTGTGAGTACTGTCAGTACCATGTCCAGGCTCAGTAC
AAGAAGCTCAGCGCAAAGCGTGCGGATCTGCAGTCCACCTTCTCTGGAGGACGAATTCCAAAGAAGTTTG
CCCGCAGAGGCACCAGCCTCAAAGAACGGCTGTGCCAAGATGGCTTTTACTACGGAGGGGTTTCTTCTGC
CTCGTATGCAGCTTCAATTGCAGCAGCTGTGGCTCCTAAGAAGAAGATTCAAACCACTCTGAGTAATCTG
GTTGTTAAGGGCACAAACTTGATCATCCAGGAAACACGGCAAAAACTCGGAATACCCCAGAAGAGCCTGT
CTTGCTCTGAGGAGTTCAAGGAACTGATGGACCTGCCGACGTGTGGAGCCAGGAACTTAAAACAACATTT
AGCCAAAGCCACAGCTTCAGGGATTATGGGGAGCCCAAAACCAGCCATCAAGTCCATCTCGGCCTCAGCA
CTCTTGAAGCAACAGAAGCAGCGGATGTTGGAGATGAGGAGAAGGAAATCAGAAGAAATACAGAAGCGAT
TTCTGCAGAGCTCAAGTGAAGTTGAGAGCCCAGCTGTGCCATCTTCATCAAGACAGCCCCCTGCTCAGCC
TCCACGGACAGGATCCGAGTTCCCCAGGCTGGAGGGAGCCCCGGCCACAATGACGCCCAAGCTGGGGCGA
GGTGTCTTGGAAGGAGATGATGTTCTCTTTTATGATGAGTCACCACCACCAAGACCAAAACTGAGTGCTT
TAGCAGAAGCCAAAAAGTTAGCTGCTATCACCAAATTAAGGGCAAGAGGCCAGGTTCTTACAAAAACAAA
CCCAAACAGCATTAAGAAGAAACAAAAGGACCCTCAGGACATCCTGGAGGTGAAGGAACGTGTAGAAAAA
AACACCATGTTTTCTTCTCAAGCTGAGGATGAATTGGAGCCTGCCAGGAAAAAAAGGAGAGAACAACTTG
CCTATCTGGAATCTGAGGAATTTCAGAAAATCCTAAAAGCAAAATCAAAACACACAGGCATCCTGAAAGA
GGCCGAGGCTGAGATGCAGGAGCGCTACTTTGAGCCACTGGTGAAAAAAGAACAAATGGAAGAAAAGATG
AGAAACATCAGAGAAGTGAAGTGCCGTGTCGTGACATGCAAGACGTGCGCCTATACCCACTTCAAGCTGC
TGGAGACCTGCGTCAGTGAGCAGCATGAATACCACTGGCATGATGGTGTGAAGAGGTTTTTCAAATGTCC
CTGTGGAAACAGAAGCATCTCCTTGGACAGACTCCCGAACAAGCACTGCAGTAACTGTGGCCTCTACAAA
TGGGAACGGGACGGAATGCTAAAGGAAAAGACTGGTCCAAAGATAGGAGGAGAAACTCTGTTACCAAGAG
GAGAAGAACATGCTAAATTTCTGAACAGCCTTAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210997 protein sequence
Red=Cloning site Green=Tags(s)

MDEEEDNLSLLTALLEENESALDCNSEENNFLTRENGEPDAFDELFDADGDGESYTEEADDGETGETRDE
KENLATLFGDMEDLTDEEEVPASQSTENRVLPAPAPRREKTNEELQEELRNLQEQMKALQEQLKVTTIKQ
TASPARLQKSPVEKSPRPPLKERRVQRIQESTCFSAELDVPALPRTKRVARTPKASPPDPKSSSSRMTSA
PSQPLQTISRNKPSGITRGQIVGTPGSSGETTQPICVEAFSGLRLRRPRVSSTEMNKKMTGRKLIRLSQI
KEKMAREKLEEIDWVTFGVILKKVTPQSVNSGKTFSIWKLNDLRDLTQCVSLFLFGEVHKALWKTEQGTV
VGILNANPMKPKDGSEEVCLSIDHPQKVLIMGEALDLGTCKAKKKNGEPCTQTVNLRDCEYCQYHVQAQY
KKLSAKRADLQSTFSGGRIPKKFARRGTSLKERLCQDGFYYGGVSSASYAASIAAAVAPKKKIQTTLSNL
VVKGTNLIIQETRQKLGIPQKSLSCSEEFKELMDLPTCGARNLKQHLAKATASGIMGSPKPAIKSISASA
LLKQQKQRMLEMRRRKSEEIQKRFLQSSSEVESPAVPSSSRQPPAQPPRTGSEFPRLEGAPATMTPKLGR
GVLEGDDVLFYDESPPPRPKLSALAEAKKLAAITKLRARGQVLTKTNPNSIKKKQKDPQDILEVKERVEK
NTMFSSQAEDELEPARKKRREQLAYLESEEFQKILKAKSKHTGILKEAEAEMQERYFEPLVKKEQMEEKM
RNIREVKCRVVTCKTCAYTHFKLLETCVSEQHEYHWHDGVKRFFKCPCGNRSISLDRLPNKHCSNCGLYK
WERDGMLKEKTGPKIGGETLLPRGEEHAKFLNSLK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018518
ORF Size 2625 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018518.5
RefSeq Size 4559 bp
RefSeq ORF 2625 bp
Locus ID 55388
UniProt ID Q7L590
Cytogenetics 10p13
Protein Families Stem cell - Pluripotency
MW 98.2 kDa
Gene Summary The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Other Versions

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