TEC (NM_003215) Human Tagged ORF Clone

CAT#: RC210992

TEC (Myc-DDK-tagged)-Human tec protein tyrosine kinase (TEC)

Product Images

Circular map for RC210992

• 

  Western blot validation of overexpression lysate (Cat# LY418830) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210992 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TEC
• Synonyms: PSCTK4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210992 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTTTAACACTATTTTGGAGGAGATTCTTATTAAAAGGTCACAGCAGAAAAAGAAGACATCGCCCT
  TAAACTACAAAGAGAGACTTTTTGTACTTACAAAGTCCATGCTAACCTACTATGAGGGTCGAGCAGAGAA
  GAAATACAGAAAGGGGTTTATTGATGTTTCAAAAATCAAGTGTGTGGAAATAGTGAAGAATGATGATGGT
  GTCATTCCCTGTCAAAATAAGTATCCATTTCAGGTTGTTCATGATGCTAACACACTTTACATTTTTGCAC
  CTAGTCCACAAAGCAGGGACCTGTGGGTGAAGAAGTTAAAAGAAGAAATAAAGAACAACAATAATATTAT
  GATTAAATATCATCCTAAATTCTGGACAGATGGAAGTTATCAGTGTTGTAGACAAACTGAAAAATTAGCA
  CCCGGATGTGAAAAATACAATCTTTTTGAGAGCAGTATAAGAAAAGCACTACCTCCAGCACCAGAAACAA
  AGAAGCGAAGGCCTCCCCCACCAATTCCACTAGAAGAAGAAGATAATAGTGAAGAAATCGTTGTAGCCAT
  GTATGATTTCCAAGCAGCAGAAGGACATGATCTCAGATTAGAGAGAGGCCAAGAGTATCTCATTTTAGAA
  AAGAATGATGTTCATTGGTGGAGAGCAAGAGATAAATATGGGAATGAAGGATATATCCCAAGTAATTACG
  TAACGGGAAAGAAATCAAACAACTTAGATCAATATGAATGGTATTGCAGAAATATGAATAGAAGCAAGGC
  AGAGCAACTCCTCCGCAGTGAAGATAAAGAAGGTGGTTTTATGGTAAGGGATTCCAGTCAACCAGGCTTG
  TACACAGTCTCCCTTTATACCAAGTTTGGAGGAGAAGGTTCATCGGGTTTTAGGCATTATCATATAAAGG
  AAACAACAACATCTCCAAAGAAGTATTACCTAGCTGAAAAACATGCTTTTGGCTCCATTCCTGAGATTAT
  TGAATATCATAAGCACAATGCAGCAGGACTTGTCACCAGGCTTCGGTACCCAGTTAGTGTGAAAGGGAAG
  AATGCACCCACCACTGCAGGATTCAGCTATGAGAAATGGGAGATTAACCCTTCAGAACTGACCTTTATGA
  GGGAATTGGGAAGTGGACTGTTTGGAGTGGTGAGGCTTGGCAAATGGCGAGCCCAGTACAAAGTCGCAAT
  CAAAGCTATTCGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAAAGTGATGATGAAACTG
  ACACACCCGAAGTTAGTGCAGCTTTATGGTGTGTGCACCCAGCAGAAACCAATATACATTGTTACTGAGT
  TCATGGAAAGGGGCTGCCTTCTGAATTTCCTCCGACAGAGACAAGGTCATTTCAGTAGAGACGTACTGCT
  GAGCATGTGTCAGGATGTGTGTGAAGGGATGGAGTATCTGGAGAGAAACAGCTTCATCCACAGAGATCTG
  GCTGCCAGAAATTGTCTAGTAAGTGAGGCGGGAGTTGTAAAAGTATCTGATTTTGGAATGGCCAGGTATG
  TTCTGGATGATCAGTACACAAGTTCTTCTGGTGCTAAGTTTCCTGTGAAGTGGTGTCCACCTGAAGTGTT
  TAATTACAGCCGCTTCAGCAGCAAATCAGATGTCTGGTCATTTGGTGTTTTAATGTGGGAAGTATTCACG
  GAAGGCAGAATGCCTTTTGAAAAATACACCAATTATGAAGTGGTAACCATGGTTACTCGAGGCCACCGAC
  TCTACCAGCCGAAGTTGGCGTCCAACTATGTGTATGAGGTGATGCTGAGATGTTGGCAGGAGAAACCAGA
  GGGAAGGCCTTCTTTCGAAGATCTGCTGCGCACAATAGATGAACTAGTTGAATGTGAAGAAACTTTTGGA
  AGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210992 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNFNTILEEILIKRSQQKKKTSPLNYKERLFVLTKSMLTYYEGRAEKKYRKGFIDVSKIKCVEIVKNDDG
  VIPCQNKYPFQVVHDANTLYIFAPSPQSRDLWVKKLKEEIKNNNNIMIKYHPKFWTDGSYQCCRQTEKLA
  PGCEKYNLFESSIRKALPPAPETKKRRPPPPIPLEEEDNSEEIVVAMYDFQAAEGHDLRLERGQEYLILE
  KNDVHWWRARDKYGNEGYIPSNYVTGKKSNNLDQYEWYCRNMNRSKAEQLLRSEDKEGGFMVRDSSQPGL
  YTVSLYTKFGGEGSSGFRHYHIKETTTSPKKYYLAEKHAFGSIPEIIEYHKHNAAGLVTRLRYPVSVKGK
  NAPTTAGFSYEKWEINPSELTFMRELGSGLFGVVRLGKWRAQYKVAIKAIREGAMCEEDFIEEAKVMMKL
  THPKLVQLYGVCTQQKPIYIVTEFMERGCLLNFLRQRQGHFSRDVLLSMCQDVCEGMEYLERNSFIHRDL
  AARNCLVSEAGVVKVSDFGMARYVLDDQYTSSSGAKFPVKWCPPEVFNYSRFSSKSDVWSFGVLMWEVFT
  EGRMPFEKYTNYEVVTMVTRGHRLYQPKLASNYVYEVMLRCWQEKPEGRPSFEDLLRTIDELVECEETFG
  R
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003215
• ORF Size: 1893 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003215.3
• RefSeq Size: 3620 bp
• RefSeq ORF: 1896 bp
• Locus ID: 7006
• UniProt ID: P42680
• Cytogenetics: 4p12-p11
• Domains: pkinase, SH2, TyrKc, SH3, BTK, PH, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: T cell receptor signaling pathway
• MW: 73.6 kDa
• Gene Summary: The protein encoded by this gene belongs to the Tec family of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. Tec family kinases are involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. They are also key players in the regulation of the immune functions. Tec kinase is an integral component of T cell signaling and has a distinct role in T cell activation. This gene may be associated with myelodysplastic syndrome. [provided by RefSeq, Jul 2008]