TEC (NM_003215) Human Tagged ORF Clone

CAT#: RC210992

Reviews ()
Write a review

TEC (Myc-DDK-tagged)-Human tec protein tyrosine kinase (TEC)



  "NM_003215" in other vectors (7)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TEC
Synonyms PSCTK4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210992 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATTTTAACACTATTTTGGAGGAGATTCTTATTAAAAGGTCACAGCAGAAAAAGAAGACATCGCCCT
TAAACTACAAAGAGAGACTTTTTGTACTTACAAAGTCCATGCTAACCTACTATGAGGGTCGAGCAGAGAA
GAAATACAGAAAGGGGTTTATTGATGTTTCAAAAATCAAGTGTGTGGAAATAGTGAAGAATGATGATGGT
GTCATTCCCTGTCAAAATAAGTATCCATTTCAGGTTGTTCATGATGCTAACACACTTTACATTTTTGCAC
CTAGTCCACAAAGCAGGGACCTGTGGGTGAAGAAGTTAAAAGAAGAAATAAAGAACAACAATAATATTAT
GATTAAATATCATCCTAAATTCTGGACAGATGGAAGTTATCAGTGTTGTAGACAAACTGAAAAATTAGCA
CCCGGATGTGAAAAATACAATCTTTTTGAGAGCAGTATAAGAAAAGCACTACCTCCAGCACCAGAAACAA
AGAAGCGAAGGCCTCCCCCACCAATTCCACTAGAAGAAGAAGATAATAGTGAAGAAATCGTTGTAGCCAT
GTATGATTTCCAAGCAGCAGAAGGACATGATCTCAGATTAGAGAGAGGCCAAGAGTATCTCATTTTAGAA
AAGAATGATGTTCATTGGTGGAGAGCAAGAGATAAATATGGGAATGAAGGATATATCCCAAGTAATTACG
TAACGGGAAAGAAATCAAACAACTTAGATCAATATGAATGGTATTGCAGAAATATGAATAGAAGCAAGGC
AGAGCAACTCCTCCGCAGTGAAGATAAAGAAGGTGGTTTTATGGTAAGGGATTCCAGTCAACCAGGCTTG
TACACAGTCTCCCTTTATACCAAGTTTGGAGGAGAAGGTTCATCGGGTTTTAGGCATTATCATATAAAGG
AAACAACAACATCTCCAAAGAAGTATTACCTAGCTGAAAAACATGCTTTTGGCTCCATTCCTGAGATTAT
TGAATATCATAAGCACAATGCAGCAGGACTTGTCACCAGGCTTCGGTACCCAGTTAGTGTGAAAGGGAAG
AATGCACCCACCACTGCAGGATTCAGCTATGAGAAATGGGAGATTAACCCTTCAGAACTGACCTTTATGA
GGGAATTGGGAAGTGGACTGTTTGGAGTGGTGAGGCTTGGCAAATGGCGAGCCCAGTACAAAGTCGCAAT
CAAAGCTATTCGGGAAGGTGCAATGTGCGAGGAGGACTTTATAGAAGAAGCTAAAGTGATGATGAAACTG
ACACACCCGAAGTTAGTGCAGCTTTATGGTGTGTGCACCCAGCAGAAACCAATATACATTGTTACTGAGT
TCATGGAAAGGGGCTGCCTTCTGAATTTCCTCCGACAGAGACAAGGTCATTTCAGTAGAGACGTACTGCT
GAGCATGTGTCAGGATGTGTGTGAAGGGATGGAGTATCTGGAGAGAAACAGCTTCATCCACAGAGATCTG
GCTGCCAGAAATTGTCTAGTAAGTGAGGCGGGAGTTGTAAAAGTATCTGATTTTGGAATGGCCAGGTATG
TTCTGGATGATCAGTACACAAGTTCTTCTGGTGCTAAGTTTCCTGTGAAGTGGTGTCCACCTGAAGTGTT
TAATTACAGCCGCTTCAGCAGCAAATCAGATGTCTGGTCATTTGGTGTTTTAATGTGGGAAGTATTCACG
GAAGGCAGAATGCCTTTTGAAAAATACACCAATTATGAAGTGGTAACCATGGTTACTCGAGGCCACCGAC
TCTACCAGCCGAAGTTGGCGTCCAACTATGTGTATGAGGTGATGCTGAGATGTTGGCAGGAGAAACCAGA
GGGAAGGCCTTCTTTCGAAGATCTGCTGCGCACAATAGATGAACTAGTTGAATGTGAAGAAACTTTTGGA
AGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210992 protein sequence
Red=Cloning site Green=Tags(s)

MNFNTILEEILIKRSQQKKKTSPLNYKERLFVLTKSMLTYYEGRAEKKYRKGFIDVSKIKCVEIVKNDDG
VIPCQNKYPFQVVHDANTLYIFAPSPQSRDLWVKKLKEEIKNNNNIMIKYHPKFWTDGSYQCCRQTEKLA
PGCEKYNLFESSIRKALPPAPETKKRRPPPPIPLEEEDNSEEIVVAMYDFQAAEGHDLRLERGQEYLILE
KNDVHWWRARDKYGNEGYIPSNYVTGKKSNNLDQYEWYCRNMNRSKAEQLLRSEDKEGGFMVRDSSQPGL
YTVSLYTKFGGEGSSGFRHYHIKETTTSPKKYYLAEKHAFGSIPEIIEYHKHNAAGLVTRLRYPVSVKGK
NAPTTAGFSYEKWEINPSELTFMRELGSGLFGVVRLGKWRAQYKVAIKAIREGAMCEEDFIEEAKVMMKL
THPKLVQLYGVCTQQKPIYIVTEFMERGCLLNFLRQRQGHFSRDVLLSMCQDVCEGMEYLERNSFIHRDL
AARNCLVSEAGVVKVSDFGMARYVLDDQYTSSSGAKFPVKWCPPEVFNYSRFSSKSDVWSFGVLMWEVFT
EGRMPFEKYTNYEVVTMVTRGHRLYQPKLASNYVYEVMLRCWQEKPEGRPSFEDLLRTIDELVECEETFG
R

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003215
ORF Size 1893 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003215.3
RefSeq Size 3620 bp
RefSeq ORF 1896 bp
Locus ID 7006
UniProt ID P42680
Cytogenetics 4p12-p11
Domains pkinase, SH2, TyrKc, SH3, BTK, PH, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways T cell receptor signaling pathway
MW 73.6 kDa
Gene Summary The protein encoded by this gene belongs to the Tec family of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. Tec family kinases are involved in the intracellular signaling mechanisms of cytokine receptors, lymphocyte surface antigens, heterotrimeric G-protein coupled receptors, and integrin molecules. They are also key players in the regulation of the immune functions. Tec kinase is an integral component of T cell signaling and has a distinct role in T cell activation. This gene may be associated with myelodysplastic syndrome. [provided by RefSeq, Jul 2008]

Other Versions

Frequently bought together (5)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 30 ul

USD 165.00


pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 627.00


Rabbit polyclonal TEC Antibody (Center)
    • 400 ul

USD 512.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 149.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 55.00

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.