GPR10 (PRLHR) (NM_004248) Human Tagged ORF Clone

CAT#: RC210917

PRLHR (Myc-DDK-tagged)-Human prolactin releasing hormone receptor (PRLHR)

Product Images

Circular map for RC210917

• 

  Western blot validation of overexpression lysate (Cat# LY418118) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210917 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPR10
• Synonyms: GPR10; GR3; PrRPR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210917 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCATCGACCACTCGGGGCCCCAGGGTTTCTGACTTATTTTCTGGGCTGCCGCCGGCGGTCACAA
  CTCCCGCCAACCAGAGCGCAGAGGCCTCGGCGGGCAACGGGTCGGTGGCTGGCGCGGACGCTCCAGCCGT
  CACGCCCTTCCAGAGCCTGCAGCTGGTGCATCAGCTGAAGGGGCTGATCGTGCTGCTCTACAGCGTCGTG
  GTGGTCGTGGGGCTGGTGGGCAACTGCCTGCTGGTGCTGGTGATCGCGCGGGTGCGCCGGCTGCACAACG
  TGACGAACTTCCTCATCGGCAACCTGGCCTTGTCCGACGTGCTCATGTGCACCGCCTGCGTGCCGCTCAC
  GCTGGCCTATGCCTTCGAGCCACGCGGCTGGGTGTTCGGCGGCGGCCTGTGCCACCTGGTCTTCTTCCTG
  CAGCCGGTCACCGTCTATGTGTCGGTGTTCACGCTCACCACCATCGCAGTGGACCGCTACGTCGTGCTGG
  TGCACCCGCTGAGGCGGCGCATCTCGCTGCGCCTCAGCGCCTACGCTGTGCTGGCCATCTGGGCGCTGTC
  CGCGGTGCTGGCGCTGCCCGCCGCCGTGCACACCTATCACGTGGAGCTCAAGCCGCACGACGTGCGCCTC
  TGCGAGGAGTTCTGGGGCTCCCAGGAGCGCCAGCGCCAGCTCTACGCCTGGGGGCTGCTGCTGGTCACCT
  ACCTGCTCCCTCTGCTGGTCATCCTCCTGTCTTACGTCCGGGTGTCAGTGAAGCTCCGCAACCGCGTGGT
  GCCGGGCTGCGTGACCCAGAGCCAGGCCGACTGGGACCGCGCTCGGCGCCGGCGCACCTTCTGCTTGCTG
  GTGGTGGTCGTGGTGGTGTTCGCCGTCTGCTGGCTGCCGCTGCACGTCTTCAACCTGCTGCGGGACCTCG
  ACCCCCACGCCATCGACCCTTACGCCTTTGGGCTGGTGCAGCTGCTCTGCCACTGGCTCGCCATGAGTTC
  GGCCTGCTACAACCCCTTCATCTACGCCTGGCTGCACGACAGCTTCCGCGAGGAGCTGCGCAAACTGTTG
  GTCGCTTGGCCCCGCAAGATAGCCCCCCATGGCCAGAATATGACCGTCAGCGTGGTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210917 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASSTTRGPRVSDLFSGLPPAVTTPANQSAEASAGNGSVAGADAPAVTPFQSLQLVHQLKGLIVLLYSVV
  VVVGLVGNCLLVLVIARVRRLHNVTNFLIGNLALSDVLMCTACVPLTLAYAFEPRGWVFGGGLCHLVFFL
  QPVTVYVSVFTLTTIAVDRYVVLVHPLRRRISLRLSAYAVLAIWALSAVLALPAAVHTYHVELKPHDVRL
  CEEFWGSQERQRQLYAWGLLLVTYLLPLLVILLSYVRVSVKLRNRVVPGCVTQSQADWDRARRRRTFCLL
  VVVVVVFAVCWLPLHVFNLLRDLDPHAIDPYAFGLVQLLCHWLAMSSACYNPFIYAWLHDSFREELRKLL
  VAWPRKIAPHGQNMTVSVVI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004248
• ORF Size: 1110 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004248.2
• RefSeq Size: 1980 bp
• RefSeq ORF: 1113 bp
• Locus ID: 2834
• UniProt ID: P49683
• Cytogenetics: 10q26.11
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 41.1 kDa
• Gene Summary: PRLHR is a 7-transmembrane domain receptor for prolactin-releasing hormone (PRLH; MIM 602663) that is highly expressed in anterior pituitary (Ozawa et al., 2002 [PubMed 11923475]).[supplied by OMIM, Mar 2008]