AVPR V2 (AVPR2) (NM_000054) Human Tagged ORF Clone
AVPR2 (Myc-DDK-tagged)-Human arginine vasopressin receptor 2 (AVPR2), transcript variant 1
|Product Name||AVPR V2 (AVPR2) (NM_000054) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||ADHR; DI1; DIR; DIR3; NDI; V2R|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC210914 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC210914 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1113 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_000054.1, NM_000054.2, NM_000054.3, NM_000054.4, NP_000045.1|
|RefSeq Size||1682 bp|
|RefSeq ORF||1116 bp|
|Protein Families||Druggable Genome, GPCR, Transmembrane|
|Protein Pathways||Neuroactive ligand-receptor interaction|
|Gene Summary||This gene encodes the vasopressin receptor, type 2, also known as the V2 receptor, which belongs to the seven-transmembrane-domain G protein-coupled receptor (GPCR) superfamily, and couples to Gs thus stimulating adenylate cyclase. The subfamily that includes the V2 receptor, the V1a and V1b vasopressin receptors, the oxytocin receptor, and isotocin and mesotocin receptors in non-mammals, is well conserved, though several members signal via other G proteins. All bind similar cyclic nonapeptide hormones. The V2 receptor is expressed in the kidney tubule, predominantly in the distal convoluted tubule and collecting ducts, where its primary property is to respond to the pituitary hormone arginine vasopressin (AVP) by stimulating mechanisms that concentrate the urine and maintain water homeostasis in the organism. When the function of this gene is lost, the disease Nephrogenic Diabetes Insipidus (NDI) results. The V2 receptor is also expressed outside the kidney although its tissue localization is uncertain. When these 'extrarenal receptors' are stimulated by infusion of a V2 selective agonist (dDAVP), a variety of clotting factors are released into the bloodstream. The physiologic importance of this property is not known - its absence does not appear to be detrimental in NDI patients. The gene expression has also been described in fetal lung tissue and lung cancer associated with alternative splicing. [provided by RefSeq, Jul 2008]|
|The use of this cDNA Clones has been cited in the following citations:|
Synthesis, Pharmacological and Structural Characterization of Novel Conopressins from Conus miliaris
,Giribaldi, J;Ragnarsson, L;Pujante, T;Enjalbal, C;Wilson, D;Daly, NL;Lewis, RJ;Dutertre, S;,
Mar Drugs 2020
,PubMed ID 32155768
|cDNA Clone Resources|
|SC115220||AVPR2 (untagged)-Human arginine vasopressin receptor 2 (AVPR2), transcript variant 1||
|RG210914||AVPR2 (GFP-tagged) - Human arginine vasopressin receptor 2 (AVPR2), transcript variant 1||
|RC210914L1||Lenti ORF clone of Human arginine vasopressin receptor 2 (AVPR2), transcript variant 1, Myc-DDK-tagged||
|RC210914L2||Lenti ORF clone of Human arginine vasopressin receptor 2 (AVPR2), transcript variant 1, mGFP tagged||
|RC210914L3||Lenti ORF clone of Human arginine vasopressin receptor 2 (AVPR2), transcript variant 1, Myc-DDK-tagged||
|RC210914L4||Lenti ORF clone of Human arginine vasopressin receptor 2 (AVPR2), transcript variant 1, mGFP tagged||