EDAR (NM_022336) Human Tagged ORF Clone

CAT#: RC210862

EDAR (Myc-DDK-tagged)-Human ectodysplasin A receptor (EDAR)

Product Images

Circular map for RC210862

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  Western blot validation of overexpression lysate (Cat# LY402917) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC210862 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified EDAR protein (Cat# TP310862). The protein was produced from HEK293T cells transfected with EDAR cDNA clone (Cat# RC210862) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EDAR
• Synonyms: DL; ECTD10A; ECTD10B; ED1R; ED3; ED5; EDA-A1R; EDA1R; EDA3; HRM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210862 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCATGTGGGGGACTGCACGCAGACGCCCTGGCTCCCCGTCCTGGTGGTGTCTCTGATGTGCTCAG
  CCCGAGCGGAATACTCAAACTGCGGTGAGAACGAGTACTACAACCAGACTACGGGGCTGTGCCAGGAGTG
  CCCCCCGTGTGGGCCGGGAGAGGAGCCCTACCTGTCCTGTGGCTACGGCACCAAAGACGAGGACTACGGC
  TGCGTCCCCTGCCCGGCGGAGAAGTTTTCCAAAGGAGGCTACCAGATATGCAGGCGTCACAAAGACTGTG
  AGGGCTTCTTCCGGGCCACCGTGCTGACACCAGGGGACATGGAGAATGACGCTGAGTGTGGCCCTTGCCT
  CCCTGGCTACTACATGCTGGAGAACAGACCGAGGAACATCTATGGCATGGTCTGCTACTCCTGCCTCCTG
  GCACCCCCCAACACCAAGGAATGTGTGGGAGCCACTTCAGGAGCTTCTGCCAACTTCCCTGGCACCTCGG
  GCAGCAGCACCCTGTCTCCCTTCCAGCACGCCCACAAAGAACTCTCAGGCCAAGGACACCTGGCCACTGC
  CCTGATCATTGCAATGTCCACCATCTTCATCATGGCCATCGCCATCGTCCTCATCATCATGTTCTACATC
  CTGAAGACAAAGCCCTCTGCCCCAGCCTGTTGCACCAGCCACCCGGGGAAGAGCGTGGAGGCCCAAGTGA
  GCAAGGACGAGGAGAAGAAAGAGGCCCCAGACAACGTGGTGATGTTCTCTGAGAAGGATGAATTTGAGAA
  GCTGACAGCAACTCCAGCAAAGCCCACCAAGAGCGAGAACGATGCCTCATCCGAGAATGAGCAGCTGCTG
  AGCCGGAGCGTCGACAGTGATGAGGAGCCCGCCCCTGACAAGCAGGGCTCCCCGGAGCTGTGCCTGCTGT
  CGCTGGTTCACCTGGCCAGGGAGAAGTCTGCCACCAGCAACAAGTCAGCCGGGATTCAAAGCCGGAGGAA
  AAAGATCCTCGATGTGTATGCCAACGTGTGTGGAGTCGTGGAAGGTCTTAGCCCCACGGAGCTGCCATTT
  GATTGTCTCGAGAAGACTAGCCGAATGCTCAGCTCCACGTACAACTCTGAGAAGGCTGTTGTGAAAACGT
  GGCGCCACCTCGCCGAGAGCTTCGGCCTGAAGAGGGATGAGATTGGGGGCATGACAGACGGCATGCAACT
  CTTTGACCGCATCAGCACGGCAGGCTACAGCATCCCTGAGCTACTCACAAAACTGGTGCAGATTGAGCGG
  CTGGATGCTGTGGAGTCCTTGTGTGCAGACATACTGGAGTGGGCGGGGGTTGTGCCACCTGCCTCCCAGC
  CACATGCTGCATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210862 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAHVGDCTQTPWLPVLVVSLMCSARAEYSNCGENEYYNQTTGLCQECPPCGPGEEPYLSCGYGTKDEDYG
  CVPCPAEKFSKGGYQICRRHKDCEGFFRATVLTPGDMENDAECGPCLPGYYMLENRPRNIYGMVCYSCLL
  APPNTKECVGATSGASANFPGTSGSSTLSPFQHAHKELSGQGHLATALIIAMSTIFIMAIAIVLIIMFYI
  LKTKPSAPACCTSHPGKSVEAQVSKDEEKKEAPDNVVMFSEKDEFEKLTATPAKPTKSENDASSENEQLL
  SRSVDSDEEPAPDKQGSPELCLLSLVHLAREKSATSNKSAGIQSRRKKILDVYANVCGVVEGLSPTELPF
  DCLEKTSRMLSSTYNSEKAVVKTWRHLAESFGLKRDEIGGMTDGMQLFDRISTAGYSIPELLTKLVQIER
  LDAVESLCADILEWAGVVPPASQPHAAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022336
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022336.4
• RefSeq Size: 4226 bp
• RefSeq ORF: 1347 bp
• Locus ID: 10913
• UniProt ID: Q9UNE0
• Cytogenetics: 2q13
• Domains: DEATH
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction
• MW: 48.6 kDa
• Gene Summary: This gene encodes a member of the tumor necrosis factor receptor family. The encoded transmembrane protein is a receptor for the soluble ligand ectodysplasin A, and can activate the nuclear factor-kappaB, JNK, and caspase-independent cell death pathways. It is required for the development of hair, teeth, and other ectodermal derivatives. Mutations in this gene result in autosomal dominant and recessive forms of hypohidrotic ectodermal dysplasia. [provided by RefSeq, Jul 2008]