LIF (NM_002309) Human Tagged ORF Clone
LIF (Myc-DDK-tagged)-Human leukemia inhibitory factor (cholinergic differentiation factor) (LIF)
|Type||Human Tagged ORF Clone|
|Synonyms||CDF; DIA; HILDA; MLPLI|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC210818 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC210818 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||606 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||3987 bp|
|RefSeq ORF||609 bp|
|Protein Families||Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein|
|Protein Pathways||Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway|
|Gene Summary||The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]|
|cDNA Clone Resources|
|RC210818L1||Lenti ORF clone of Human leukemia inhibitory factor (cholinergic differentiation factor) (LIF), Myc-DDK-tagged||
|RC210818L2||Lenti ORF clone of Human leukemia inhibitory factor (cholinergic differentiation factor) (LIF), mGFP tagged||
|RC210818L3||Lenti ORF clone of Human leukemia inhibitory factor (cholinergic differentiation factor) (LIF), Myc-DDK-tagged||
|RC210818L4||Lenti ORF clone of Human leukemia inhibitory factor (cholinergic differentiation factor) (LIF), mGFP tagged||
|RG210818||LIF (GFP-tagged) - Human leukemia inhibitory factor (cholinergic differentiation factor) (LIF)||
|SC303176||LIF (untagged)-Human leukemia inhibitory factor (cholinergic differentiation factor) (LIF)||